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Citations for Qiagen :
1 - 50 of 10000+ citations for Human Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Total RNA from eukaryotic cells was isolated using the RNeasy mini kit (QIAGEN). RNA was transcribed using the iScript reverse transcriptase (BioRad ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml binding buffer (provided in Qiagen PCR purification kit, #28106) and 20 µl of 3 M sodium acetate pH 5.5 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... and His6-4E-BP1 was purified using Ni-NTA agarose (#1018244, QIAGEN) and eluted with 250 mM imidazole (#A1073 ...
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bioRxiv - Cell Biology 2023Quote: ... and His6-4E-BP1 was purified using Ni-NTA agarose (#1018244, QIAGEN) and eluted with 250 mM imidazole (#A1073 ...
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bioRxiv - Cell Biology 2022Quote: The heatmaps relative to the ERK-dependent AP-1 target genes were generated by interrogating the Transcription Factor Target (TFT) collection (MSigDB) and TF binding site database by QIAGEN with the top DEGs between control and PD-treated Acomys ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The mei-P26[1] and OreR samples were processed to cDNA using random hexamers and ribosomal sequences were depleted with sequential eukaryotic and bacterial rRNA depletion kits (Qiagen FastSelect). Illumina dual-indexed libraries were made from these cDNAs and sequenced as 2x150bp reads.
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bioRxiv - Cancer Biology 2023Quote: Total RNA samples were extracted from D2A1-M1 tumor samples collected at the end of the 2-week drug treatment experiment (Fig. 4e) using a powered tissue homogenizer (OMNI Tissue Master 125) and RNeasy Plus Mini Kits (Qiagen, 74134) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and binding of sample to an RNeasy mini kit column (Qiagen). RNA was washed and eluted according to the manufacturer’s instructions and the concentration was measured using a NanoDrop 2000 (Thermo Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... RNA binding and purification was performed using RNeasy Mini Kit (Qiagen). Total RNA was extracted with RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA were labeled by nick translation to generate green fluorescent probes using Atto488 Nick Translation Labeling Kit (Jenabioscience) and purified by silica-gel membrane adsorption (Qiagen PCR Purification Kit), according to the kit protocols ...
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bioRxiv - Physiology 2019Quote: ... in 40 μl Binding Buffer (Pyromark binding buffer, Qiagen, Manchester UK) and 25 μl Milli-Q-water ...
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bioRxiv - Microbiology 2024Quote: Eukaryotic RNA was isolated from mouse tissue samples (stomach and colon) stored in RNAprotect Tissue Reagent (Qiagen) at ™70 °C before extraction using a GeneJET RNA Purification Kit (Cat ...
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bioRxiv - Molecular Biology 2024Quote: DNA-FISH probes were obtained after nick translation of BAC constructs (RP11-45IJN, RP5-915N17) purified using the Large Construct kit (Qiagen): 1 μg of purified DNA was labelled for 3 h at 16° C with fluorescent dUTPs (SEEBRIGHT Orange 552 dUTP and SEEBRIGHT Green 496 dUTP ...
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bioRxiv - Molecular Biology 2024Quote: ... PyroMark binding buffer (Qiagen), and 1 μL Streptavidin sepharose TM high performance beads (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... and microbial Eukaryotic abundance with a QIAcuity One 5-plex digital PCR (dPCR) instrument (Qiagen Inc. USA, Germantown, MD). dPCR samples represented both the 416 Fire —across SBS ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat. Nr. 85300). Lysates were span down at 20.000 rcf for 20 minutes at 4°C to remove debris ...
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bioRxiv - Physiology 2020Quote: ... or the QuantiTect PCR Kit with self-designed primers for neuronal factors (Qiagen, Germany) (Supplementary Table 3) ...
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bioRxiv - Genomics 2023Quote: ... Reactions were stopped by adding the appropriate volume of Binding Buffer (Qiagen MinElute PCR Kit) and the DNA was purified using the Qiagen MinElute PCR Kit according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and H2A.Z (fused with a maltose-binding protein -MBP) purification were performed by affinity chromatography using Ni-NTA agarose (Qiagen) and amylose resin (NEB) ...
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bioRxiv - Bioengineering 2021Quote: ... supernatant was collected and Factor Xa was removed with Factor Xa Removal Resin (QIAGEN). Endotoxin was removed using endotoxin removal resin and Atsttrin purity was determined by SDS-PAGE and reverse phase high performance liquid chromatography (HPLC).
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bioRxiv - Genetics 2021Quote: Multi-array ELISA was performed using the Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The assessment of ligand binding was performed via shifts in protein thermostability upon binding using thermal denaturation on a rotary quantitative PCR (qPCR) instrument (Qiagen Rotor-Gene Q 2plex HRM ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using a 10:1 ratio of binding buffer (a modified version of Qiagen’s PB buffer) to digestive mixture ...
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bioRxiv - Biochemistry 2020Quote: ... After binding to Ni-NTA Superflow (Qiagen) resin ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from INS-1 and human islets 48 hours post transfection using RNeasy cleanup kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Protein extraction was conducted using a Qproteome Bacterial Protein Prep Kit (Qiagen)‡ ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein isolation with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Genomics 2019Quote: ... The mixture was then passed through the RNA binding column of RNeasy Mini Kit (74104, Qiagen, Germantown, MD), followed by washing and elution steps described in the manufacture’s handbook.
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bioRxiv - Immunology 2023Quote: ... RNA from human PBMCs was isolated using RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: AllPrep DNA/RNA/Protein Mini Kits (Qiagen) were used to extract total proteins from UVA-exposed and non-exposed HTEpC from all experiments ...
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bioRxiv - Evolutionary Biology 2020Quote: ... purification step with 10x binding buffer PB (Qiagen) was carried out between the first and second steps.
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bioRxiv - Genetics 2020Quote: ... 440 μl of DNA binding buffer beads (Qiagen) were added to the sample and mixed by pipetting ...
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bioRxiv - Immunology 2022Quote: ... coli lysate by binding to Ni-NTA (QIAGEN) and gravity chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... 440 μl of DNA binding buffer beads (Qiagen) were added to the sample and mixed by pipetting ...
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bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein were extracted using the AllPrep DNA/RNA/Protein kit (Qiagen, #47054). Sample concentrations were measured with Qubit high sensitivity dsDNA and RNA platform ...
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bioRxiv - Microbiology 2021Quote: ... Frozen lung sections were homogenized in 1 ml of Lysis/Binding solution in a TissueLyser II (Qiagen) with three cycles of at 30 Hz for 30 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... human THP-1 macrophages (MACs) and acutely isolated mouse microglia was extracted using the RNeasy Plus Mini kit (Qiagen, 74136) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... astrocytes and human brain tissue using the RNeasy Mini kit (Qiagen) and reverse transcribed using the High-Capacity RNA-to-cDNA kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein was extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen #80004) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was purified by the AllPrep DNA/RNA/Protein Mini Kit (Qiagen: # 80004). Corresponding protein expression levels in the cells of different groups were detected by western blot using the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested for total protein using a Qproteome Mammalian Protein Prep Kit (Qiagen). Total protein was quantified using a PierceTM BCA Protein Assay Kit (Cat ...
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bioRxiv - Cancer Biology 2020Quote: The All Prep DNA/RNA/Protein kit (QIAGEN) was used to total RNA from cell lines ...
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bioRxiv - Neuroscience 2023Quote: RNA from human microglia differentiated in vitro and human microglia extracted from human-mouse chimeras was extracted using the RNeasy Plus Micro Kit (Qiagen). The RNA from 3 independent in vitro microglia differentiations per cell line were pooled at equal weight to obtain six samples ...
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bioRxiv - Neuroscience 2019Quote: ... 200 μl of ethanol was added to the samples prior to binding to Qlamp spin column (QIAamp DNA Blood mini kit; Qiagen) and samples were centrifuged at 8000 rpm for 1 minute ...
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bioRxiv - Microbiology 2020Quote: ... VSG2-antibody negative clones (positive transfectants were identified by lack of binding) were sequenced: RNA was isolated using the RNeasy Mini Kit including an on-column DNase treatment (Qiagen). Complementary DNA was synthesized with SuperScript IV Reverse Transcriptase (Invitrogen ...