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Citations for Qiagen :
1 - 50 of 397 citations for Human Differentiation Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and human induced astrocytes (>day 30 of differentiation) were harvested in RLTplus Lysis buffer (Qiagen, 1053393). Total RNA was isolated using the RNeasy micro/mini plus kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... and ImmunoCult™ Human Treg Differentiation Supplement (StemCell TechnoRNA was isolated at set time points after activation using the RNeasy Mini (Qiagen). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (ThermoFisher) ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was isolated from approximately 100 EBs (differentiation day 2 and 3) or 25 EBs (differentiation day 4 and 5) using RNeasy Mini kit (Qiagen). 0.5 μg RNA was transcribed to cDNA using QuantiTect Reverse Transcription kit (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... 8 and 10 of differentiation as recommended (RNAeasy Minikit. Qiagen 74106). Mineralized nodule formation was measured by staining cultures with Alizarin Red (40 mM ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: Cell samples from two independent differentiations were collected in Buffer RLT (QIAGEN) at the the time points described above (Figure 1A) ...
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bioRxiv - Cell Biology 2022Quote: ... MZ and MM iHep differentiations were isolated in triplicate using the miRNeasy kit (Qiagen) per manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: Total RNA was extracted once a day during differentiation using the RNeasy kit (QIAGEN). RNA was converted to cDNA using the SmartSeq2 protocol (Picelli et al ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339, human METTL3: QIAGEN SI05020414 and human DDX6 ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339 ...
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bioRxiv - Genetics 2022Quote: iPSC-CMs were harvested at day 30 of differentiation following the RNeasy Minikit protocol (Qiagen). RT-PCR was performed as above with variant-specific primers for each step (Table S1) ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757 ...
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bioRxiv - Neuroscience 2019Quote: RNA was extracted from cells on day 37 of neuronal differentiation using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA from approximately 25 EBs at day 5 of differentiation was isolated using RNeasy Mini kit (Qiagen) and quantified by NanoDrop (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2023Quote: RNA was extracted at different time points during EpiSC differentiation to PPs using the RNeasy extraction kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was harvested at 70% confluency or after differentiation using the RNEasy Mini kit (Qiagen #74104, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated from ∼100,000 MSNs from 4 independent differentiations per experimental group according to the manufacturer’s protocol and purified using RNeasy columns (Qiagen). A total of 72 mRNA libraries for Illumina sequencing were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: Quantitative real-time PCR for stemness and differentiation genes was performed using primers purchased from Qiagen (QuantiTect primer assay). RNA was isolated from cells according to the manufacturer’s instructions using Trizol (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from approximately 25-50 EBs at day 5 of differentiation was isolated using RNeasy Mini kit (Qiagen) and quantified by NanoDrop (Thermo Fisher) ...
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bioRxiv - Genetics 2022Quote: ... human SETD2 siRNA (Qiagen # 1027416 ...
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bioRxiv - Genetics 2022Quote: ... human CRY2 siRNA (Qiagen # 1027416 ...
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bioRxiv - Genetics 2022Quote: ... human CPSF6 siRNA (Qiagen # 1027416 ...
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bioRxiv - Biophysics 2019Quote: ... Total RNA was isolated from all generated cell lines (Table S1) at day 9 of differentiation using either the RNeasy Mini kit or the AllPrep DNA/RNA Mini kit (Qiagen). ATAC-seq in four cell lines (KO ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was extracted from cells on fibers on days 14 and 28 of CM differentiation using a RNeasy Mini Kit (Qiagen), according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: 3×106 cells from the day 8 of CD34+ HSPC and day 6 of HUDEP-2 erythroid differentiation were used for total RNA using RNeasy Mini Kit (Qiagen). For reverse transcription using Primescript RT reagent kit (Takara Bio Inc.) ...
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bioRxiv - Cell Biology 2020Quote: Human DUOX1: GPH1004464(-)02A (Qiagen)
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bioRxiv - Cell Biology 2020Quote: Human DUOX2: GPH1018346(-)08A (Qiagen)
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bioRxiv - Cell Biology 2020Quote: ... Human RPLP0 (primers from QIAGEN) was used as reference gene for cDNA from Caco-2 samples ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715 ...
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bioRxiv - Cancer Biology 2023Quote: ... with Quantitect human primers (Qiagen) for genes ID1 ...
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bioRxiv - Developmental Biology 2023Quote: ... BeWo conditioned media and MEHP (10μM) + BeWo conditioned media) using the RNeasy PLUS Mini Kit from Qiagen (Germantown, MD). RNA extraction followed the manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: hESCs were collected from various stages of directed differentiation and subjected to RNA extraction using the RNeasy Mini Kit (QIAGEN 74106). Reverse transcription was performed with the Clontech RT-PCR kit ...
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bioRxiv - Cell Biology 2022Quote: ... and organoids at the end of passage one subjected to two weeks of differentiation (n=3) using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: RNA was collected from iPSCs at d0 and iPSC-derived neurons at d32 and d60 of differentiation (Qiagen RNeasy Kit, QIAshredder) and processed with on-column DNase treatment to remove genomic DNA ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: Total RNA was extracted from day 0 to day 7 of myotube differentiation using an RNeasy Mini Kit (Qiagen, Cat.# 74104) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: RNA extraction was performed on cells from individual wells of a 24-well tissue-culture dish at each differentiation time-point using an RNEasy mini kit (Qiagen 74104). cDNA was generated from RNA using an Applied Biosystems High Capacity cDNA Reverse Transcription kit (ThermoFisher 4368814) ...
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bioRxiv - Neuroscience 2022Quote: ... for the isogenic pair (APOE3 and APOE4) of iMGLs exposed to spheroid conditioned media or unspent neuronal media was achieved with RNeasy Plus Mini Kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Immunology 2023Quote: ... the media was aspirated and RLT buffer (Qiagen) was added to the wells ...
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bioRxiv - Cell Biology 2020Quote: ... RNA from three independent biological experiments were collected at different time points before and after induction of differentiation including (d0-d6) the extraction was completed using RNeasy Mini Kit (QIAGEN, Cat. 74104). RNA quality of all samples (n=7 time points and n=3 experiments from independent passages ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from hiPSCs at given time points of hepatocyte differentiation using the QIAshredder and RNeasy mini kits (Qiagen, Germantown, MD). Complementary DNA (cDNA ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from all samples (5 CTRL and 5 SCZ subjects at six timepoints across astrocyte differentiation) using either the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) or the MagMax mirVana Total RNA Isolation Kit (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... human myosin-18A (synthesized by Qiagen)
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bioRxiv - Microbiology 2021Quote: ... 40 μl of media from each of 6 plant wells or media from 3 minimal media wells were pooled and stabilized in RNAprotect® Bacteria Reagent (QIAGEN) before performing RNA extraction using RNeasy Mini Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA for ANXA2 (Human, Qiagen, Hs_ANXA2_8, SI02632385) or siRNA for Anxa2 (Mouse ...
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bioRxiv - Neuroscience 2023Quote: RNA from human microglia differentiated in vitro and human microglia extracted from human-mouse chimeras was extracted using the RNeasy Plus Micro Kit (Qiagen). The RNA from 3 independent in vitro microglia differentiations per cell line were pooled at equal weight to obtain six samples ...
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bioRxiv - Microbiology 2022Quote: The human Hh signaling target PCR array (Qiagen) profiled the expression of 84 key genes responsive to Hh signal transduction ...
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bioRxiv - Cell Biology 2020Quote: ... Human cell motility RT2 profiler PCR Array (Qiagen) for control-MPC and Givi-MPC was performed ...
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bioRxiv - Microbiology 2021Quote: Human colon resections were placed in RNAlater (Qiagen) at the time of resection ...
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bioRxiv - Microbiology 2023Quote: The human Hippo signaling target PCR array (Qiagen) was used to determine expression of 84 key Hippo target genes ...
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bioRxiv - Microbiology 2023Quote: Human Inflammatory Response & Autoimmunity Focus) (Qiagen, YAHS-205Z) were used for each of the different samples ...
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bioRxiv - Physiology 2024Quote: ... and Qiagen human Primer Assays (Qiagen, Hilden, Germany) specific for FoxO1 and FoxO3 (the latter only in NHBEs) ...