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Citations for Qiagen :
1 - 50 of 478 citations for Human Coronavirus NL63 Nuceloprotein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... or pQE30 vectors (expressed in M15 E. coli cells (Qiagen)) ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µg C19orf43 (purified from E. coli BL21 through Qiagen Ni-TA agarose using standard procedures ...
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bioRxiv - Biochemistry 2019Quote: Mutant Chinese hamster BiP proteins with an N-terminal His6-tag were purified as described before with modifications (Preissler et al, 2017b). Proteins were expressed in M15 Escherichia coli (E. coli) cells (Qiagen). The bacterial cultures were grown in LB medium supplemented with 100 µg/ml ampicillin and 50 µg/ml kanamycin at 37 ° C to an OD600nm of 0.8 and expression was induced with 1 mM IPTG ...
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bioRxiv - Microbiology 2024Quote: ... Verified plasmids were extracted from Escherichia coli (E. coli) using the QIAprep Spin Miniprep Kit or HiSpeed Plasmid Midi Kit (Qiagen) and dialyzed (0.025µM MCE Membrane ...
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bioRxiv - Microbiology 2020Quote: Coronavirus nsp12 sequence alignment was generated using CLC Workbench (Qiagen) from sequences downloaded from the NCBI website (Accession numbers MN985325.1 and MT123290.1 ...
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bioRxiv - Microbiology 2022Quote: ... 1mg of bacterial genomic DNA (isolated from E. coli cells using genomic DNA kit, Qiagen), or tRNA (from E ...
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bioRxiv - Microbiology 2021Quote: 3-5 x 105 cells infected or not with SARS-CoV-2 or HCoV-NL63 were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
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bioRxiv - Cell Biology 2021Quote: The GST fusion proteins were expressed in bacteria (E. coli, BL21) and purified by glutathione-Sepharose (Cat No. 27-4574-01, Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Bacterial clones on selection plates were scraped and pooled to extract recombinant plasmids containing the recipient barcodes and DNA blocks (donor barcodes, oligonucleotides, and E. coli ORFs) using Plasmid Plus Mini Kit (QIAGEN). The pooling capacity is determined by the number of unique positioning barcodes ...
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bioRxiv - Biochemistry 2024Quote: ... coli (QIAGEN) or phage T4 SSB (gp32 ...
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bioRxiv - Molecular Biology 2021Quote: Beta-coronavirus RNA was extracted from the viral samples on each petri dish using the QiAmp Viral RNA mini kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (Qiagen) to generate the protein expression strains ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Qiagen). The bacterial cultures were grown in LB medium with 100 μg/ml ampicillin and 50 μg/ml kanamycin at 37°C to an OD600 nm of 0.8 and expression was induced with 1 mM isopropylthio β-D-1-galactopyranoside (IPTG) ...
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bioRxiv - Biophysics 2021Quote: ... coli M15 (Qiagen) was transformed with ApsAC (1-577):pQE70-2Z.4 and grown in LB media to OD600 ≈0.7 ...
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bioRxiv - Microbiology 2023Quote: The amino acid sequences of coronavirus spike orthologues were subjected to multiple alignment using CLC Workbench 7 (CLC Bio, Qiagen, Aarhus, Denmark). Protein sequence NCBI reference sequences for the indicated viruses are as follows ...
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bioRxiv - Cell Biology 2022Quote: ... coli M15 cells (Qiagen) with pRAREII (Novagen).
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bioRxiv - Biochemistry 2020Quote: ... coli M15 (pREP4) (Qiagen), fresh transformants were inoculated into LB liquid culture (1000 mL ...
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bioRxiv - Molecular Biology 2021Quote: ... coli M15 cells (Qiagen) using H2O-based M9 medium supplemented with 15NH4Cl and D-glucose-13C6-d7 ...
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bioRxiv - Microbiology 2022Quote: ... coli by miniprep (Qiagen), and the internal 504 bp of pilT was removed by digestion with AccI (New England Biolabs) ...
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bioRxiv - Immunology 2023Quote: ... coli strain M15 (Qiagen), and recombinant proteins were produced from the E ...
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bioRxiv - Biophysics 2020Quote: ... coli expression vector pQE80 (Qiagen). Expression of TEV protease and I86-HaloTag-TEV-I87 was induced in BLR (DE3 ...
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bioRxiv - Microbiology 2021Quote: ... coli strain M15 [pREP4] (Qiagen) containing a pQE-30 derivative encoding His6-PA1048 ...
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bioRxiv - Cell Biology 2021Quote: ... coli BLR(pREP4) cells (Qiagen) cultured in DYT medium (1% tryptone ...
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bioRxiv - Biochemistry 2021Quote: ... coli strain M15 [pREP4] (Qiagen) containing pAJD2948 was grown in 500 mL of LB broth at 37°C to an OD600 of 0.6-1.0 ...
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bioRxiv - Microbiology 2023Quote: ... coli strain M15 [pREP4] (Qiagen) containing plasmid pAJD3179 encoding His6-PA3978 was grown in LB broth to mid-log phase at 37°C with aeration ...
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bioRxiv - Microbiology 2023Quote: ... coli strain M15 [pREP4] (Qiagen) containing a plasmid pQE-30 protein-encoding derivative was grown in LB broth to mid-log phase at 37°C with aeration ...
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bioRxiv - Biochemistry 2023Quote: ... coli from pQE80L-Navi (Qiagen) as N-terminally His-tagged and biotinylated proteins for SPR ...
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bioRxiv - Microbiology 2020Quote: ... coli with QIAprep Spin Miniprep Kit (Qiagen) and were confirmed by sequencing ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... coli and purified with NiNTA matrix (Qiagen). Immunization of the rabbits was performed by Absea (Beijing ...
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bioRxiv - Microbiology 2019Quote: ... coli using the QiaPrep Spin kit (Qiagen). DNA samples were analysed by agarose gel electrophoresis and visualised by staining with SYBR Safe (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... coli and purified by Ni-NTA (QIAGEN). Then protein samples were desalted through a PD-10 column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... coli using QIAprep Spin Miniprep kits (Qiagen) as outlined by the manufacturer ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli DH5α cells and isolated by Qiagen miniprep kit (Qiagen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli DH5α using Plasmid Miniprep Kit (Qiagen) for transformation of B ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339, human METTL3: QIAGEN SI05020414 and human DDX6 ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339 ...
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bioRxiv - Developmental Biology 2021Quote: ... coli and purified using a standard protocol (QIAGEN) at a temperature lower or equal to 12 °C to obtain a near 100 % supercoiled DNA ...
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bioRxiv - Microbiology 2022Quote: ... coli strains containing expression plasmid pQE-9 (Qiagen), pQE9-ftsA ...
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bioRxiv - Genetics 2022Quote: ... coli and was purified using Ni-NTA (Qiagen) before injection ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells using an RNeasy mini kit (Qiagen) after lysis with lysozyme (FujiFilm Wako ...
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bioRxiv - Biochemistry 2021Quote: ... Escherichia coli SSB (EcoSSB) was purchased from Qiagen.
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bioRxiv - Immunology 2022Quote: ... coli lysate by binding to Ni-NTA (QIAGEN) and gravity chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified using the MiniPrep Kit (Qiagen), and sequences were confirmed by Sanger sequencing (Quintara Biosciences Service) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli and purified using the MiniPrep Kit (Qiagen), and sequences were confirmed by Sanger sequencing (Quintara Biosciences Service) ...
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bioRxiv - Biochemistry 2023Quote: ... coli and plasmids were recovered by miniprep (Qiagen). Plasmids were checked by Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2024Quote: ... coli using the QIAprep Spin Miniprep Kit (Qiagen). All plasmids made by PCR cloning were sequenced by Azenta ...
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bioRxiv - Microbiology 2021Quote: ... coli using the QIAprep Spin Miniprep kit (Qiagen, #27104). Genomic DNA (gDNA ...
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bioRxiv - Microbiology 2020Quote: ... coli by affinity chromatography using Ni-NTA agarose (Qiagen) according to the manufacturer’s instructions (Ruc-His6 was purified under urea denaturing conditions) ...
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bioRxiv - Microbiology 2020Quote: ... coli using the QIAprep Miniprep kit (QIAGEN, Germantown, MD) and confirmed by PCR to contain the construct again using the CjNC110F2 and CjNC110R2 primers.
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bioRxiv - Microbiology 2022Quote: ... coli via QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) prior to restriction enzyme digestion ...