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Citations for Qiagen :
1 - 50 of 10000+ citations for Glutathione Fluorescent Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Condensin I was bound to Glutathione Sepharose 4B beads (Cytiva) in a 5 mL polypropylene column (Qiagen) using GST- tagged mSMC4 and eluted by PreScission Protease (Cytiva ...
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bioRxiv - Genomics 2021Quote: ... The scramble-miR miRCURY LNA Detection probe (5’-GTGTAACACGTCTATACGCCCA-3’, YD00699004, QIAGEN) was used as a negative control ...
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bioRxiv - Genetics 2020Quote: ... The 3’ and 5’ – DIG labeled miRCURY LNA miRNA Detection probes (Qiagen, negative control Scramble-miR cat ...
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bioRxiv - Immunology 2021Quote: ... Plates contained 5 µl of TCL lysis buffer (Qiagen) supplemented with 1%β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... NSP1 fraction was loaded on Glutathione HiCap resin (Qiagen) equilibrated with the dialysis buffer and proteins were eluted by the same buffer supplemented with 50 mM glutathione ...
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bioRxiv - Genetics 2021Quote: ... 5 mM Multiplex-Kit (Qiagen) and HPLC water to a total volume of 10 μL per sample ...
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bioRxiv - Genetics 2019Quote: ... in 384 well plates in a total volume of 5 μl with 2.5 μl Qiagen Multiplex PCR Plus Kit (Qiagen, Hilden, Germany) and 1 μl Qiagen Q-solution using a Yakima Yellow-labelled primer ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were immobilized on a glutathione agarose gravity flow column (Qiagen) washed 5 times with PBS solution and eluted in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2020Quote: Cells seeded in 6 well plates were grown in 5% FBS to a confluency of 70% before RNA extraction using the RNEasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and sequential further passage through glutathione-Sepharose 4B and Ni-NTA (QIAGEN) resins ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of targets was performed with miRCURY LNA SYBR® Green PCR Kits (Qiagen) according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... using SYBR-Green based detection system with QuantiNova SYBR green PCR Kit (Qiagen, 208052). Relative expression of mRNA was calculated through ΔΔCt method using the ribosomal protein RP49 RNA expression as internal control for each sample ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of targets was performed with miRCURY LNA SYBR® Green PCR Kits (Qiagen) according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescent cones and rods were sorted directly into RLT-Buffer from the RNeasy kit (Qiagen, #74104) for subsequent RNA extraction.
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bioRxiv - Systems Biology 2019Quote: ... The SWIM plates were purified (Qiagen, PCR Purification Kit) and processed to make an Illumina sequencing library ...
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bioRxiv - Plant Biology 2020Quote: ... and the GST fusion proteins were purified by Glutathione HiCap Matrix columns (Qiagen) and eluted using 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Briefly proteins were purified by glutathione-Sepharose (Cytiva) or Ni-NTA agarose (Qiagen) affinity chromatography ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions were eluted using 10 mM reduced glutathione through a polypropylene column (Qiagen), and those with the highest concentrations combined ...
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bioRxiv - Cell Biology 2022Quote: ... single cells were sorted into PCR plates containing 5 µl Buffer RLT Plus (Qiagen) with 1% BME and immediately frozen at -80°C for G&T sequencing.
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bioRxiv - Cancer Biology 2020Quote: ... for candidate mRNA detection by use of the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen), following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... for candidate mRNA detection by use of the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen), following manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... all libraries were quantified using the highly sensitive fluorescent dye-based Qubit ds DNA Assay Kit (Qiagen). Whole RNA library preparation was performed using the TruSeq stranded mRNA HT technology (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... or the DNeasy® 96 Blood & Tissue plate kit (Qiagen) according to supplier’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA were labeled by nick translation to generate green fluorescent probes using Atto488 Nick Translation Labeling Kit (Jenabioscience) and purified by silica-gel membrane adsorption (Qiagen PCR Purification Kit), according to the kit protocols ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified with glutathione Sepharose beads (GE) or Ni-NTA spin columns (Qiagen), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified with glutathione Sepharose beads (GE) or Ni-NTA spin columns (Qiagen), respectively ...
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bioRxiv - Genetics 2021Quote: ... The western blot analysis was performed according to the QIAexpress detection kit and the protocol (Qiagen) with the following modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... The QuantiTect SYBR® Green PCR Kit was used for this biomolecular detection (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2024Quote: ... Amplification and detection were performed using the QIAcuityTM OneStep Advanced Probe Kit (Qiagen, Cat. No. 250132), with primers and probe targeting the influenza A virus M gene segment (specific amplicon length is 110 bp) ...
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bioRxiv - Genomics 2019Quote: ... phagocytosed by sorting cells into a 96 well plate containing 5 ul of RLT (Qiagen) + 1%β-Mercaptoethanol (Sigma).
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bioRxiv - Immunology 2022Quote: ... single-cells were sorted in 96-well plates containing 5 µL of TCL buffer (Qiagen) with 1% β-mercaptoethanol according to the gating strategy shown in Fig ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of Taq PCR Master Mix kit (Qiagen), 0.4 μl of each primer (100 pmol/μl ...
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bioRxiv - Microbiology 2021Quote: ... NG1 (5’ACCGACCACAGGGGG-3’) and NG2 (5’-GGTTGTAAACCTCTTTCGA-3’) and HotStarTaq® Master Mix Kit (Qiagen) up to a final volume of 25 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... the mixture was incubated with Glutathione Sepharose™ 4B (Cytiva) and Ni-NTA Superflow (Qiagen). The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
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bioRxiv - Immunology 2023Quote: Total mRNA was isolated from 5×105 cells using a kit (RNeasy kit, QIAGEN) and reverse-transcribed using oligo(dT)20 primer (SuperscriptIII ...
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bioRxiv - Microbiology 2020Quote: ... 5) Powersoil® Isolation kit (MO Bio Laboratories/Qiagen, Canada) with modifications ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was extracted using the RNeasy 96-well plate kit (Qiagen, #74181) according to manufacturer’s instructions including the DNase step ...
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bioRxiv - Molecular Biology 2019Quote: ... The desired ~3 kb product was separated on a 1x TBE agarose gel and recovered by electroelution and ethanol precipitation (fluorescent DNA substrates) or with the QIAquick gel extraction kit (Qiagen).
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bioRxiv - Developmental Biology 2023Quote: ... at 300 cells per well into 96 well LoBind plates containing 5 μL of EB buffer (Qiagen). After sorting ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was isolated from Hep3B cell spheroids on the pillar plate and in the ULA plate using a RNeasy Plus kit (Qiagen,74134). One μg of RNA was converted into cDNA using the high-capacity cDNA reverse transcription kit according to the manufacturer instructions (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: Cytokine- or vehicle-stimulated primary CD1 astrocyte cultures in 6-well plates and neuron cultures in 24-well plates were lysed and their total RNA extracted using RNeasy Mini Kits (Qiagen 74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA from 10 adult flies (5 males/5 females) was extracted using DNeasy Blood & Tissue Kit (Qiagen). DNA was resuspended and sheared in 1X TE (0.1 mM EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... and passed through 1 mL of glutathione–Sepharose 4B and then onto a Ni-NTA column (QIAGEN) to capture the residual GST- and His-tag fragments ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was incubated under gentle shaking overnight with glutathione matrix (Qiagen; 1ml per 1000ml cell culture) at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and 5 cells per well were sorted into a 96-well plate containing TCL buffer (Qiagen, cat. 1031576) with 1 % beta- Mercaptethanol and snap frozen on dry ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Plates were then placed at 4 0C before adding the reverse transcription mix containing 5’-biotinylated TSO (Qiagen). PCR products were cleaned up using 0.8:1 Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: RNA was extracted from 6-well plates using miRNeasy Mini Kits (QIAGEN 217004) with the inclusion of an on-column DNase digestion (QIAGEN 79254) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted directly from the plates using RNeasy Mini Kit (Qiagen, 74104). 2μg of RNA were used for cDNA synthesis using High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Free GST and thrombin were removed with a second glutathione column and a Ni-NTA column (Qiagen 30210) to remove thrombin ...
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bioRxiv - Molecular Biology 2021Quote: ... Oligonucleotides containing shRNA inserts were PCR-amplified with primers 5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’ and 5’-TGAACTCGAGAAGGTATATTGCTGTTGACAGTGAGCG-3’ and purified with QIAquick PCR Purification Kit (Qiagen). shRNA inserts and miRE18_LT3GEPIR_Ren714 backbone (inducible via Tet-On system ...