Labshake search
Citations for Qiagen :
1 - 50 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... NSP1 fraction was loaded on Glutathione HiCap resin (Qiagen) equilibrated with the dialysis buffer and proteins were eluted by the same buffer supplemented with 50 mM glutathione ...
-
bioRxiv - Cell Biology 2022Quote: ... and passed through 1 mL of glutathione–Sepharose 4B and then onto a Ni-NTA column (QIAGEN) to capture the residual GST- and His-tag fragments ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were immobilized on a glutathione agarose gravity flow column (Qiagen) washed 5 times with PBS solution and eluted in 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and sequential further passage through glutathione-Sepharose 4B and Ni-NTA (QIAGEN) resins ...
-
bioRxiv - Cancer Biology 2021Quote: ... Detection of targets was performed with miRCURY LNA SYBR® Green PCR Kits (Qiagen) according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Neuroscience 2021Quote: ... using SYBR-Green based detection system with QuantiNova SYBR green PCR Kit (Qiagen, 208052). Relative expression of mRNA was calculated through ΔΔCt method using the ribosomal protein RP49 RNA expression as internal control for each sample ...
-
bioRxiv - Cancer Biology 2021Quote: ... Detection of targets was performed with miRCURY LNA SYBR® Green PCR Kits (Qiagen) according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Neuroscience 2023Quote: ... Fluorescent cones and rods were sorted directly into RLT-Buffer from the RNeasy kit (Qiagen, #74104) for subsequent RNA extraction.
-
bioRxiv - Systems Biology 2019Quote: ... The SWIM plates were purified (Qiagen, PCR Purification Kit) and processed to make an Illumina sequencing library ...
-
bioRxiv - Plant Biology 2020Quote: ... and the GST fusion proteins were purified by Glutathione HiCap Matrix columns (Qiagen) and eluted using 50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... Briefly proteins were purified by glutathione-Sepharose (Cytiva) or Ni-NTA agarose (Qiagen) affinity chromatography ...
-
bioRxiv - Cell Biology 2021Quote: ... Fractions were eluted using 10 mM reduced glutathione through a polypropylene column (Qiagen), and those with the highest concentrations combined ...
-
bioRxiv - Cancer Biology 2020Quote: ... for candidate mRNA detection by use of the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen), following manufacturer’s protocols ...
-
bioRxiv - Cancer Biology 2022Quote: ... for candidate mRNA detection by use of the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen), following manufacturer’s protocols ...
-
bioRxiv - Neuroscience 2021Quote: ... all libraries were quantified using the highly sensitive fluorescent dye-based Qubit ds DNA Assay Kit (Qiagen). Whole RNA library preparation was performed using the TruSeq stranded mRNA HT technology (Illumina ...
-
bioRxiv - Microbiology 2021Quote: ... or the DNeasy® 96 Blood & Tissue plate kit (Qiagen) according to supplier’s protocol.
-
bioRxiv - Cell Biology 2020Quote: ... genomic DNA were labeled by nick translation to generate green fluorescent probes using Atto488 Nick Translation Labeling Kit (Jenabioscience) and purified by silica-gel membrane adsorption (Qiagen PCR Purification Kit), according to the kit protocols ...
-
bioRxiv - Cell Biology 2021Quote: ... coli and purified with glutathione Sepharose beads (GE) or Ni-NTA spin columns (Qiagen), respectively ...
-
bioRxiv - Cell Biology 2021Quote: ... coli and purified with glutathione Sepharose beads (GE) or Ni-NTA spin columns (Qiagen), respectively ...
-
bioRxiv - Genetics 2021Quote: ... The western blot analysis was performed according to the QIAexpress detection kit and the protocol (Qiagen) with the following modifications ...
-
bioRxiv - Molecular Biology 2021Quote: ... The QuantiTect SYBR® Green PCR Kit was used for this biomolecular detection (Qiagen, Hilden, Germany).
-
bioRxiv - Microbiology 2024Quote: ... Amplification and detection were performed using the QIAcuityTM OneStep Advanced Probe Kit (Qiagen, Cat. No. 250132), with primers and probe targeting the influenza A virus M gene segment (specific amplicon length is 110 bp) ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were washed 3 times with sterile 1x PBS and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed four times.
-
bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
-
bioRxiv - Molecular Biology 2023Quote: ... the mixture was incubated with Glutathione Sepharose™ 4B (Cytiva) and Ni-NTA Superflow (Qiagen). The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
-
bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from cells (1-2 wells, 6 well plate) using an RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), including a DNase step to remove residual DNA ...
-
bioRxiv - Systems Biology 2023Quote: ... RNA was extracted using the RNeasy 96-well plate kit (Qiagen, #74181) according to manufacturer’s instructions including the DNase step ...
-
bioRxiv - Molecular Biology 2019Quote: ... The desired ~3 kb product was separated on a 1x TBE agarose gel and recovered by electroelution and ethanol precipitation (fluorescent DNA substrates) or with the QIAquick gel extraction kit (Qiagen).
-
bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
-
bioRxiv - Bioengineering 2023Quote: ... total RNA was isolated from Hep3B cell spheroids on the pillar plate and in the ULA plate using a RNeasy Plus kit (Qiagen,74134). One μg of RNA was converted into cDNA using the high-capacity cDNA reverse transcription kit according to the manufacturer instructions (Applied Biosystems ...
-
bioRxiv - Neuroscience 2023Quote: Cytokine- or vehicle-stimulated primary CD1 astrocyte cultures in 6-well plates and neuron cultures in 24-well plates were lysed and their total RNA extracted using RNeasy Mini Kits (Qiagen 74104) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Condensin I was bound to Glutathione Sepharose 4B beads (Cytiva) in a 5 mL polypropylene column (Qiagen) using GST- tagged mSMC4 and eluted by PreScission Protease (Cytiva ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant was incubated under gentle shaking overnight with glutathione matrix (Qiagen; 1ml per 1000ml cell culture) at 4°C ...
-
bioRxiv - Genetics 2023Quote: RNA was extracted from 6-well plates using miRNeasy Mini Kits (QIAGEN 217004) with the inclusion of an on-column DNase digestion (QIAGEN 79254) ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted directly from the plates using RNeasy Mini Kit (Qiagen, 74104). 2μg of RNA were used for cDNA synthesis using High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... Free GST and thrombin were removed with a second glutathione column and a Ni-NTA column (Qiagen 30210) to remove thrombin ...
-
bioRxiv - Plant Biology 2020Quote: The real-time quantification of cDNA corresponding to 2µg of total RNA was performed in the ABI PRISM 7900HT sequence detection system using the QuantiTech SYBR Green RT-PCR kit (Qiagen). The Gene-specific primers used are listed in Table S1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Viral RNA was extracted one from (Used in detection limit evaluation) using viral RNeasy® Mini-kit (Qiagen, Hildan, Germany). Extracted RNA was stored at −70°C until further use.
-
bioRxiv - Systems Biology 2019Quote: ... Plates were prepared using the miRCURY SYBR Green PCR Kit (Qiagen, cat. no. 339346) with custom miRCURY LNA PCR primers (Qiagen cat ...
-
bioRxiv - Cell Biology 2023Quote: ... Complexes were pulled down using Glutathione-Sepharose 4B resin (Cytiva; 17-0756-01) or Ni-NTA resin (QIAGEN; 30210) for 2 h at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... The soluble lysates obtained after two rounds of centrifugation at 18,000g and 4°C were incubated with glutathione agarose beads (Pierce) or Ni2+-NTA beads (QIAGEN). Recombinant proteins were eluted from the beads using 10 mM reduced glutathione in a Tris (50 mM Tris-HCl ...
-
bioRxiv - Genomics 2019Quote: ... Plates grown in parallel were used for genomic DNA extraction (DNeasy Blood & Tissue kit, Qiagen), and RNA extraction (TRIzol ...
-
bioRxiv - Biophysics 2022Quote: ... Supernatants were incubated with glutathione Sepharose (GSH) fast flow beads (GE-Healthcare) for GST-tagged proteins or nickel– nitriloacetic acid (Ni-NTA) agarose (Qiagen) for His-tagged proteins for 2 h at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: The GST fusion proteins were expressed in bacteria (E. coli, BL21) and purified by glutathione-Sepharose (Cat No. 27-4574-01, Qiagen) as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was added to a 500 μl equilibrated Glutathione Sepharose 4 Fast Flow affinity medium (GE) or Ni-NTA agarose (Qiagen). The lysate was incubated with an affinity matrix for 1 hour at 4 ⁰C ...
-
bioRxiv - Microbiology 2021Quote: ... Resultant cDNAs were used as template for qPCR and qPCR was performed on an Applied Biosystems 7900HT Sequence Detection system using a Sybr Green Quantitect kit (QIAGEN, Hildesheim, Germany). Quantification cycle (Cq ...
-
bioRxiv - Biochemistry 2022Quote: ... as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000, Qiagen) for one hour ...
-
bioRxiv - Microbiology 2019Quote: ... Cignal EGR-1 reporter kit (Qiagen) was transfected in HEK293T following manufacturer protocol ...
-
bioRxiv - Genomics 2022Quote: ... The 12 wells from individual plate rows were pooled and cleaned (QIAquick PCR Purification Kit, QIAGEN), and the 30μl elutions resulting from every prep were combined and vortexed thoroughly ...
-
bioRxiv - Biochemistry 2023Quote: ... The cells were transfected using Effectene transfection kit for individual dishes or 24 well plates (Qiagen) or Linear PEI (Sigma Aldrich ...