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Citations for Qiagen :
1 - 50 of 10000+ citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... DNA extraction from the liver or fin and pectoral fin muscles of the fish from which sperm was collected were performed using DNeasy Blood & Tissue Kit (QIAGEN).
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bioRxiv - Microbiology 2020Quote: ... Total bacterial DNA was extracted from fish homogenate sample using QIAmp DNA Microbiome Kit (Qiagen) following manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: Sperm genomic DNA (gDNA) was extracted using the Gentra Puregene Cell kit (QIAGEN). In short ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: High molecular-weight sperm DNA collected from F0 males was obtained using MagAttract HMW DNA Kit (QIAGEN, Cat # 67563), following a modified version of the manufacturer’s instructions which consisted of replacing the digestion step with Proteinase K with 40 µL of the reducing agent TCEP (Tris(2-carboxyethyl)phosphine hydrochloride ...
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bioRxiv - Molecular Biology 2023Quote: Sperm DNA was extracted from ∼ 10 million spermatozoa/sample by lysing cells with RLT+ buffer (Qiagen) with 1% Beta-mercaptoethanol for 15 min. ...
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bioRxiv - Genomics 2021Quote: Total DNA was extracted from muscle tissue of one male fish using a QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Genomic DNA from both sperm and saliva samples was extracted using the Puregene Core Kit A (QIAGEN, #1042601) according to the manufacturer’s instructions with minor modifications previously described in (Arbeithuber et al. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted DNA from stored sperm using the Qiagen DNeasy Blood and Tissue Extraction kit (Qiagen, Hilden, Germany), and added 12μL DTT to each sample to improve lysis of sperm cells ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was extracted from the caudal fin of each challenged fish using the DNeasy Blood & Kit tissue kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was extracted from fin clip samples (n=768 fish) using the commercial kit DNeasy Blood & Tissue Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and 15-30 μg of DNA was extracted from ethanol-fixed fish tissues using a DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s guidelines.
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bioRxiv - Genomics 2023Quote: ... SINEB1 and two orthogonal sets of MERVL RNA FISH probes were generated using genomic DNA of E14 mESCs extracted with DNeasy Blood and Tissue Kits (Qiagen 69504) as a template for PCR ...
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bioRxiv - Immunology 2023Quote: Total DNA and mRNA were isolated from the intestine samples collected during fish necropsy with the AllPrep DNA/RNA/Protein Kit (Qiagen, Hilden, Germany), following manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: RNA was extracted from fish liver using RNeasy Plus Mini Prep Kits (QIAGEN) following manufacturer protocols ...
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bioRxiv - Genomics 2021Quote: ... FISH was performed using the miRCURY LNA miRNA ISH kit (FFPE, 339450, QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... sperm lysis buffer was prepared by combining (for each sample) 497.5 μL of Qiagen Buffer RLT (Qiagen) without beta-mercaptoethanol ...
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bioRxiv - Developmental Biology 2021Quote: 5ng/sample RNA isolated from sperm was reverse transcribed (RT) using the miCURY LNA RT kit (Qiagen #339340). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and six control fish (all females) using a standard Trizol reagent protocol (miRNeasy Micro kit, Qiagen) and stored at −80°C after verifying concentration and quality by spectrophotometer (Nanodrop ...
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was isolated from one retina per fish using RNeasy Lipid/Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 3-month-old zebrafish (1 fish/sample) using the RNeasy Mini Kit (Cat. No. 74104; Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from liver of individual fish by using RNeasy Plus Universal Mini kit (Qiagen), according to manufacturer’s instruction ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Total RNA from 57 fish was isolated from the excised tissues using the RNeasy Micro Kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from liver of each individual fish using RNeasy Plus Universal Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Single sperm or oocytes were transferred into single PCR tubes for amplification with the REPLI-g Single Cell kit (QIAGEN). RADseq libraries and whole genome libraries were prepared and sequenced on the HiSeq 3000 or NovaSeq S2 sequencing system (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... and sperm using the Bio Basic EZ-10 Spin Column Genomic Minipreps kit for animal sample according to the manufacturer’s protocol except for sperm samples for which the kit ACL lysis solution was replaced by 300 μl of RTL lysis buffer (Qiagen) and 3 μl of mercaptoethanol ...
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bioRxiv - Developmental Biology 2022Quote: RNA isolation from fish tissues was done according to the manufacturer’s protocol of the RNeasy Mini Kit (Qiagen). Tissue homogenization using ceramic beats was performed with the TissueLyser II (2 min at 30 Hz) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from 3 dpf zebrafish AB larvae (50 fish) using RNAeasy Plus Kit (Qiagen 74134). cDNA was synthesized using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: ... A total of 44 single-sperm samples were selected to undergo whole genome amplification (WGA) with the REPLI-g Single Cell Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from pools of 6-10 dissected hearts of 4-OHT-treated cmcl2/RFP and cmlc2/GFP-HRAS juvenile fish that were homogenized in Qiazol (Qiagen) with a TissueLyser (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: 40-60 fish embryos or larvae were placed into trizol reagent and total RNA was extracted using the RNeasy Mini Kit (Qiagen). 1 μg of total RNA was treated with RQ1 Rnase-free DNase (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA was extracted from zebrafish melanoma tumors (Tg[mitfa:BRAFV600E];p53-/- 17,40) and from a normal region of the same fish using the Qiagen DNeasy Blood and Tissue kit (Qiagen, Germantown, MD, #69504). The DNA was fragmented and hybridized to the array ...
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bioRxiv - Neuroscience 2019Quote: Total RNA was extracted from ten pooled larval fish or one dissected adult brain per sample (RNeasy mini Kit; Qiagen, Valencia, CA, USA) for quantitative RT-PCR (qRT-PCR) ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were then euthanized and brains removed (as above) for total RNA was extracted using the RNeasy Mini Kit (QIAGEN, Venlo, Netherlands, 74104). cDNA was synthesised from brain RNAs using the SuperScript™ III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... EVs DNA (exoDNA) and cellular DNA were extracted with QIAamp DNA Micro Kit (QIAGEN) or DNeasy tissue and blood kit (QIAGEN ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted using a DNA Kit (Qiagen). Purity and concentration were determined using a Bioanalyzer (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA Extraction buffer (Qiagen GenePure DNA Isolation Kit) was subsequently added to the tubes ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted using QIAamp DNA minikit (Qiagen) according to the manufacturer specifications ...
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The heterogeneity of the DNA damage response landscape determines patient outcomes in ovarian cancerbioRxiv - Cell Biology 2021Quote: ... Purified explant DNA (QIAamp DNA Mini, 51304; Qiagen) was assessed for purity and yield (NanoDrop ND-1000) ...
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bioRxiv - Immunology 2022Quote: ... DNA isolated by QIAamp DNA Mini Kit (Qiagen) was used as the template for qPCR ...
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bioRxiv - Molecular Biology 2024Quote: DNA extraction with EZ1 DNA Investigator kit (Qiagen) was performed according to the manufacturer’s instructions and the large volume protocol [31] ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated using QiaAmp DNA Blood Maxi or QiaAmp DNA mini kits (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... we extracted DNA with Qiagen high molecular weight DNA kit (MagAttract HMW DNA Kit - Qiagen). For the toe pads ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted by using commercial DNA extraction kit (QIAamp DNA-Mini Kit; (Qiagen®). The Qubit™ dsDNA BR Assay kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Total DNA (including integrated HIV-1 DNA and episomal HIV-1 DNA) was extracted using the QIAmp blood DNA minikit (Qiagen, Courtaboeuf, France) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... DNA was purified using QIAamp DNA Mini Kit (QIAGEN).
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bioRxiv - Molecular Biology 2019Quote: ... DNA purified using the QIAamp DNA mini kit (QIAGEN), and products analyzed using SYBR green qPCR or a 30-cycle end point PCR ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA was prepared with Endo-free DNA kit (Qiagen) and was injected at 1.5 µg/µl into the telencephalic vesicles of embryos in time-staged pregnant females using a Femtojet 4i microinjector (Eppendorf ...
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bioRxiv - Genomics 2021Quote: ... We extracted DNA using QIAamp DNA Investigator Kit (Qiagen) for Gomphillus and DNeasy Plant Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: Bacteroides DNA was extracted (QIAamp DNA Mini Kit, Qiagen).
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bioRxiv - Immunology 2019Quote: ... DNA was extracted using Qiagen DNA isolation kit (Qiagen) and collected in 50 μl TE buffer ...