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Citations for Qiagen :
1 - 42 of 42 citations for Elabela 19 32 TFA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 32 μl proteinase K (Qiagen) [21] ...
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bioRxiv - Neuroscience 2020Quote: ... DIV17-19 neurons were transfected with Attractene (Qiagen). For each Mattek dish ...
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bioRxiv - Neuroscience 2022Quote: ... 19 or by using the ExoEasy Maxi Kit (Qiagen)
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bioRxiv - Cancer Biology 2021Quote: ... a 19-mer SPRY2 target sequence (5′-AACACCAATGAGTACACAGAG-3) (QIAGEN) was used and for the control a 19-mer NSi control sequence (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 32 μL of elution buffer (Qiagen). 3’-adenylation reactions were conducted by adding 5 μL of NEB buffer 2 (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... 19/304 was extracted using the Viral RNA Mini Kit (Qiagen) and eluted into 50 µL of buffer AVE ...
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bioRxiv - Cancer Biology 2021Quote: ... was used and for the control a 19-mer NSi control sequence (QIAGEN) was used ...
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bioRxiv - Genetics 2021Quote: ... was then added to 19 µl of purified (QIAquick PCR Purification Kit, QIAGEN) and hybridised PCR product containing approximately 200 ng of the PCR product ...
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bioRxiv - Neuroscience 2020Quote: ... 19 or 29 days using RNeasy Mini kit (QIAGEN, Redwood City, CA, USA), following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or human ABCB1-transfected MDR-19 cells using the RNeasy Mini extraction kit (Qiagen). First strand cDNA synthesis was performed on 500 ng of template RNA using MMLV reverse transcriptase (fc 10 units/µl) ...
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bioRxiv - Physiology 2019Quote: ... [19] Total RNA was extracted according to the manufacturer’s instructions (Qiagen, RNeasy Lipid Tissue Mini). RNA quality was ensured using an Agilent Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were eluted from the magnetic beads with 32 μl of Buffer EB (Qiagen) and 30 μl of the eluate were transferred to a fresh 96-well plate ...
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bioRxiv - Immunology 2020Quote: ... 2 μL of cDNA was added to 19 μl PCR reaction mix so that the final reaction contained 0.2 μL (0.5 U) HotStarTaq Polymerase (Qiagen), 0.075 μL of 50 μM 3′ reverse primers ...
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bioRxiv - Molecular Biology 2023Quote: ... ARPE-19 cells were transfected with a HIF-luciferase reporter gene construct (Cignal Lenti HIF Reporter, Qiagen, Netherlands). The HIF-luciferase construct encodes a firefly luciferase gene under the control of the hypoxia response element ...
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bioRxiv - Systems Biology 2020Quote: ... 19 motifs and 4 differential motifs were analyzed to predict upstream regulators using Ingenuity Pathway Analysis (IPA, QIAGEN Inc.). Gene expression signatures indicative of HMO and motif abundance were defined as genes differentially expressed with abundance in the previous limma analysis(FDR q<0.05 and |Fold Change|>1.5).
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bioRxiv - Genomics 2020Quote: ... PCR based COVID-19 viral genome enrichment was performed using QIAseq SARS-CoV-2 Primer Panel Kit (Qiagen, Germany). A total of 98 primer pairs specific to COVID-19 were used for enrichment where primers pairs were distributed in two pools (Pool-1 and Pool-2) ...
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bioRxiv - Immunology 2022Quote: ... 2 μL of cDNA was added to 19 μL first round PCR reaction mix containing 0.5 U HotStarTaq Polymerase (Qiagen), 0.18 μM of each 3’ reverse primer ...
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bioRxiv - Microbiology 2023Quote: ... amniotic fluid) and fetal tissues as previously described (32) or manually using the QIAmp DNA minikit (Qiagen). RhCMV DNA copies were determined by QPCR targeting gB (gB QPCR ...
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bioRxiv - Immunology 2021Quote: ... Twenty consecutive 10 μm sections were placed on glass slides for subsequent IHC (sections 1-2, 7-8, 13-14, & 19-20) or placed in 600 μl buffer RLT (Qiagen) containing 1% 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA and RNA from 19 tumor and adjacent normal samples was extracted using the AllPrep DNA/RNA FFPE Kit (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 2 µL of cDNA was added to 19 µl PCR reaction mix so that the final reaction contained 0.2 µL (0.5 U) HotStarTaq Polymerase (Qiagen, cat#203607), 0.075 µL of 50 µM 3′ reverse primers ...
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bioRxiv - Genomics 2022Quote: RNA-seq data and integrated ChIP-seq/RNA-seq data were uploaded to Metascape website [19] or Ingenuity Pathway Analysis software (Qiagen) as specified ...
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bioRxiv - Microbiology 2023Quote: A 388 bp region surrounding NS4A position 19 was amplified from total RNA extracted from a subset of brains using OneStep RT-PCR (Qiagen) using the following primers ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was isolated from whole blood (or in 19 cases directly from the fibroblasts) using DNEasy Blood & Tissue Kit (Qiagen), quantified ...
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bioRxiv - Plant Biology 2019Quote: ... the pellet was dissolved at 50 °C for 15 minutes in 19 ml of Buffer G2 (Qiagen, Germantown, MD, Cat #1014636).
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bioRxiv - Genomics 2020Quote: Viral RNA was extracted from nasopharyngeal swabs of patients with COVID-19 using the EZ1 DSP Virus Kit (Qiagen, Hilden, Germany), optimized for viral and bacterial nucleic acids extractions from human specimens using magnetic bead technology ...
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bioRxiv - Plant Biology 2022Quote: ... The tissue samples were disrupted and homogenized by adding 2 stainless steel beads (SSB 32) using the TissueLyserII (Qiagen) at 20 Hz for 5 mins ...
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bioRxiv - Microbiology 2022Quote: ... salivas were collected from COVID-19 patients during onset and RNA was extracted using a QIAamp viral RNA mini kit (Qiagen, Cat# 52906) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Genomic DNA was extracted from frozen young leaves of the 199 SSD lines grown in greenhouse and directly from seeds of the remaining 19 accessions using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). It was sheared using a Covaris E220 device to fragments of ∼550 bp and PCR-free libraries were constructed using the KAPA HyperPrep Kit ...
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bioRxiv - Microbiology 2020Quote: ... and Marine Benthic Group – D in the Thermoplasmata (with the latter for core 32 only) using the Quantifast SYBRGreen kit (Qiagen) on a BioRad Opticon2 thermocycler ...
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bioRxiv - Genomics 2022Quote: ... and overlaps samples described in Williams et al.32 DNA was extracted using the DNeasy Blood & Tissue Kit from Qiagen. Nucleic acid purity was inspected with a NanoDrop spectrophotometer ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with 500 ng of pGL3- DARPP-32 plasmids and 50 ng of pRL-SV40 plasmids (renilla; internal reference control) using Polyfect transfection reagent (Qiagen). Lastly ...
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bioRxiv - Immunology 2023Quote: ... The first round of PCR was carried out using the ImmunoSEQ proprietary PCR primer mix (32 μL per sample containing 25 uL of QIAGEN 2× Multiplex PCR Master Mix ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed with 2 µL (∼16 ng) or 4 µL (∼32 ng) of BT-converted DNA with HotStar Taq Polymerase (Qiagen), depending on the genomic region ...
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bioRxiv - Developmental Biology 2024Quote: ... total RNA was extracted from newly acquired human embryonic/fetal brain samples (n=32) using the AllPrep DNA/RNA Mini Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: RNA was isolated from the mouse RPE following the methodology previously described.26 Total RNA was purified from ARPE-19 cells using the RNeasy® Mini Kit (Qiagen, Germantown, MD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Whole livers were harvested at indicated timepoints and placed into a 7 ml homogenizer tube (Omni International Cat. No. 19-651) containing 3 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Spleens and single granulomas were harvested at indicated timepoints and placed into a 1 ml homogenizer tube (Fisher Brand Cat ...
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bioRxiv - Immunology 2022Quote: ... Downstream pathway analysis was performed using the gene set enrichment analysis (GSEA) tools provided by the Broad Institute 32 or ingenuity pathway analysis (IPA) (QIAGEN Inc.) 35.
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bioRxiv - Molecular Biology 2023Quote: ... as described previously (32) except that the tissue was homogenized for 120 seconds at 30 Hz in a Tissue Lyser (TissueLyser II, Qiagen, CA, USA). Superscript II RNase H- system and Oligo dT (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: Total RNA was extracted from hypothalamus and hippocampus (n = 4 per dietary group per tissue; total 32 samples) using an All-Prep DNA/RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Sample size was based on previous RNA-Seq studies in which findings were validated using qPCR and gene perturbation experiments [6 ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from dolphin oral samples and 32 negative (PBS) controls using the DNeasy UltraClean 96 Microbial Kit (Qiagen Cat. #10196-4) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The first round of PCR was carried out using the ImmunoSEQ proprietary PCR primer mix (32 μL per sample containing 25 uL of QIAGEN 2× Multiplex PCR Master Mix, 5μL of QIAGEN 5x Q-solution and 2 μL of primer mix). A positive control reaction ...