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Citations for Qiagen :
1 - 50 of 90 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Single colonies for each point mutant were Miniprepped (Qiagen) and sequence-verified using either 5’-GCCATGAACAAGTCCGAACGCGTGGAA −3’ or 5’-GCGATATTTGTTCAACGGGTGTTGAGCAAT −3’.
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: Alanine mutants were generated by site-directed mutagenesis (Qiagen) of Fc-fused IL-1RAcP and the procurement of the alanine library from Günther et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli C mutants was extracted using DNeasy Blood & Tissue kit (Qiagen). Quality of extracted DNA was confirmed before sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Toxins were then purified from bacterial cell lysates through affinity chromatography on a Nickel-resin (Qiagen), ion exchange ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the polyhistidine-tagged toxin was loaded onto a Ni-NTA agarose column (Qiagen). The column was then washed with a 20–120 mM gradient of imidazole to remove additional contaminating proteins ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmid DNA was isolated from mutant clones using a standard protocol (QIAGEN). After paired-end Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... or its mutants with 0.2 µg of constructs using the Effectene reagent (Qiagen) according manufacturer’s protocol and were used in experiments 48-72 hours later ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was extracted from homozygous mutant leaves using the RNAeasy Mini Kit (Qiagen). The extracted RNA was treated with DNase (RQ1 RNase-Free DNase ...
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bioRxiv - Genetics 2020Quote: ... DNA sample of mutants were extracted using DNeasy Plant Mini Kit (Qiagen, Germany). The workflow of data collection and processing was summarised in Fig ...
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bioRxiv - Microbiology 2020Quote: RNA was harvested from AKI toxin-inducing culture pellets preserved in 1mL Trizol using an RNEasy kit (Qiagen) using both on-column DNase digestion (Qiagen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmid mutant libraries were purified from the bacteria colonies using Plasmid Midi Kit (Qiagen).
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bioRxiv - Neuroscience 2020Quote: ... or its mutants (200 ng) and GCaMP6 (1 μg) using the Effectene reagent (Qiagen). The human orthologue of the mouse splice variant TRPM3α2 in the pCDNA3.1(+ ...
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bioRxiv - Molecular Biology 2020Quote: ... The Exposed Cys mutant plasmid library was purified using commercially available miniprep columns (Qiagen) using growth conditions of 30°C and 130 rpm to support the propagation of large plasmids.
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bioRxiv - Microbiology 2020Quote: ... His6-tagged Lem27 or its truncation mutants was purified using Ni2+-NTA columns (Qiagen). After washing with a buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid DNA was isolated from the mutant pool by Plasmid Maxi Kit (QIAGEN) according to the manufacturer's specifications.
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bioRxiv - Biochemistry 2019Quote: ... The plasmid DNA was isolated from the mutant pool by Plasmid Maxi Kit (QIAGEN) according to the manufacturer's specifications.
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bioRxiv - Developmental Biology 2022Quote: ... Mutant genotypes were identified by collecting genomic DNA (Qiagen DNeasy Blood & Tissue kit, 69504), which was amplified using PCR (DreamTaq Master Mix (2X) ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA of mutant cells was isolated using DNA Blood Mini Kit (QIAGEN, 51106) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... pH was adjusted to 8 after resuspension to allow the binding of 6x His-tagged toxins to Ni-NTA agarose resin (reference: 30210; Qiagen). Resulting samples were passed through chromatography columns containing the Ni-NTA agarose resin and were eluted with denaturing buffer containing increasing concentrations of Imidazole (10 mM ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were transfected with hTRPM3α2-GFP or its mutants using the Effectene reagent (Qiagen). Cells were loaded with 1 μM fura-2 AM (Invitrogen ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... All transformants were pooled and mutant plasmid library was extracted by plasmid Midi prep (Qiagen) and transformed by electroporation into ΔproQ cells.
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bioRxiv - Plant Biology 2020Quote: ... For genotyping genomic DNA was isolated from the mutant plants using DNeasy plant minikit (Qiagen). Homozygous lines of the mutant plants were confirmed by running a PCR reaction on genomic DNA isolated from mutant as well as wild type plant leaves using gene specific and T-DNA left boarder primers (Supplementary table) ...
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bioRxiv - Microbiology 2022Quote: ... aureus USA300 LAC isolate and butyrate-resistant mutants using the PowerSoil Pro kit (Qiagen 47014). Overnight cultures were pelleted and suspended in 200 μL of an enzymatic lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2022Quote: Plasmids of selected mutants were extracted and purified using the QIAprep Spin Miniprep Kit (Qiagen) according to the manufactures protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA from CRISPR/Cas9 mutants was extracted with RNeasy® Plant Mini Kit (Qiagen, US) following manufacturer’s instruction ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was extracted from mutants of interest using the Puregene Cell and Tissue Kit (Qiagen), as previously described (Lehrbach et al. ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: Mutant libraries of early variants were generated in the pQE-31 expression vector (Qiagen, Hilden, Germany) and expressed in SURE E ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was extracted from VSV-SARS- CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: Subdued expression in knockout mutant and RNAi expressing larvae was measured using RNeasy Mini Kit (Qiagen 74104) isolation from 3-4 larvae per genotype ...
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bioRxiv - Plant Biology 2019Quote: ... DNA was isolated from the candidate mutants using a Qiagen plant genomic DNA kit (Qiagen, Valencia, CA). DNA was subjected to WGS using an Illumina HiSeq 2500 sequencer (2×125 bp) ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from WCS417 and the 10E10 EMS mutant with the Puregene Core Kit (Qiagen). A PE150 short insert library was prepared and sequenced on an Illumina HiSeq 2500 (Novogene) ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae D39 WT and all FQ-resistant mutants using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s recommendations for Gram-positive bacteria ...
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bioRxiv - Microbiology 2020Quote: ... we isolated the genomic DNA from cell pellets of the mutant libraries with the DNeasy kit (Qiagen) and followed published protocol to generate Illumina compatible sequencing libraries [63 ...
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bioRxiv - Microbiology 2021Quote: ... we isolated the genomic DNA from cell pellets of the mutant libraries with the DNeasy kit (Qiagen) and followed published protocol to generate Illumina compatible sequencing libraries(Wetmore et al. ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 S mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... total RNA was extracted from wild-type and mutant nodules using the RNeasy Mini kit (QIAGEN, Germany) and treated with DNase (Turbo DNase kit ...
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bioRxiv - Plant Biology 2019Quote: RNA was extracted from pollen from approximately 300 flowers of WT and dgk4-1 mutants (Qiagen, USA) and cDNA synthesized using SuperScript III reverse transcriptase according to manufacturer’s instructions (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 S mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: Total RNA extraction was performed from the wild-type and mutant clones using RNeasy plus mini kit (QIAGEN). RNA was quantified by qubit 4 ...
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bioRxiv - Developmental Biology 2020Quote: mRNA was isolated from sinaEMS mutant larvae using RNeasy Mini Kit according to manufacturer’s instructions (Qiagen. Germantown, MD). cDNA synthesis was carried out using AMV First Strand cDNA Synthesis Kit following manufacturer’s instructions (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... FasRΔ33 or FasR point mutants were separated from whole-cell lysates by Ni-NTA agarose chromatography (Qiagen, Inc). After three washing steps with lysis buffer ...
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bioRxiv - Genomics 2023Quote: Genome DNA was extracted from the young leaves of Micro-Tom mutants using DNeasy Plant Mini Kit (Qiagen), and subjected to library preparation with the SPRIworks System I for the Illumina sequencer (Beckman Coulter ...
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bioRxiv - Biophysics 2019Quote: ... The cells were transiently transfected with cDNA encoding the rat TRPV2 and its mutants using the Effectene reagent (Qiagen) 48-72 hours before experiments ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated from selected putative mutants and the parent strains using the DNeasy Blood & Tissue kit (Qiagen). Library preparation and 2×150 bp paired-end Illumina sequencing was performed by the Microbial Genome Sequencing Center (Pittsburgh ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from adult WT and homozygous CatSpert-134del mutant male testes using RNeasy Mini kit (QIAGEN). 500 ng of the extracted RNA was used for cDNA synthesis using iScriptTM cDNA Synthesis kit (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from 3 dpf and 6 dpf cdipt mutant zebrafish and their wildtype siblings using RNAeasy (Qiagen). RNA samples were reverse transcribed into cDNA using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... Selected transposon mutants were grown overnight and the genomic DNA was harvested using DNeasy blood and tissue kit (Qiagen). The genomic DNA was then sent for whole genome sequencing at the Mutualized platform for Microbiology of Institut Pasteur.