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Citations for Qiagen :
1 - 50 of 58 citations for Dehydroepiandrosterone sulfate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... 0.005% sodium dodecyl sulfate) supplemented with 5LμL of Proteinase K (Qiagen). The cells were lysed by incubation at 55L°C for 1Lh ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... 0.005% sodium dodecyl sulfate) supplemented with 5LμL of Proteinase K (Qiagen) after the growth medium was removed ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Anions and Ammonium sulfate suites (Qiagen). Diffraction quality crystals of OXA-14 apo-form (PDB code 7L5R ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Genomic DNA was extracted and purified from mouse tails by a sodium hydroxide method or by proteinase K digestion followed by high-salt precipitation (Gentra/Qiagen, Valencia, CA, USA). In total ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We extracted and purified genomic DNA from tail tissue either using a sodium hydroxide method (Truett, Heeger et al. 2000) or by proteinase K digestion followed by high-salt precipitation (Gentra/Qiagen, Valencia, CA, USA). We measured the concentration and purity of DNA using a small-volume spectrophotometer (Nanodrop ...
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bioRxiv - Immunology 2020Quote: HPLC purified and salt-exchanged GapmeR (Exiqon/Qiagen) antisense oligonucleotides were incubated with T-cells at the indicated concentration for the indicated time ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA was eluted in high-salt buffer (Buffer QF) (QIAGEN). Using isopropanol precipitation ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA was eluted with a high-salt buffer (Buffer QF) (QIAGEN), desalted and concentrated using isopropanol precipitation and resuspended in 10 mM Tris-HCl (pH 8.5) ...
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bioRxiv - Microbiology 2021Quote: ... then sodium bisulfite-converted (EpiTect Bisulfite kit, Qiagen). The 5’LTR or the rev regions were amplified by (semi)nested PCR (primer sequences are available upon request) ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted with a salt-based method and an RNAse A (Qiagen) treatment was applied following the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% N-Lauroylsarcosine salt and 0.2% Triton-X100 and passed through Miniprep (QIAGEN) silica-fibre membrane spin columns in order to bind only the Top2cc’s and not DNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and treated the pools with sodium bisulfite (EpiTect, Qiagen) following a protocol for formalin-fixed paraffin-embedded samples (Gu et al ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 2 mL salts mixed with 4 mL of RNAprotect Bacteria Reagent (Qiagen), and then incubated for 5 min at room temperature for stabilization before centrifugation at 4000 rpm for 10 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted with a salt-based method (Aljanabi & Martinez 1997) and an RNAse A (Qiagen) treatment was applied following the manufacturer’s recommendation ...
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bioRxiv - Plant Biology 2022Quote: ... and sodium bisulfite treated using the Epitect Bisulfite Conversion kit (QIAGEN). The region of interest within the MeSWEET10a promoter was amplified using the primers in Extended Data Table 3 ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were collected continuously into 50 mL conicals containing 20 mL RNAlater (saturated ammonium sulfate, 20mM EDTA, pH 5.2; QIAGEN #76106) and a stirring magnetic stir disc (V&P Scientific #772DP-N42-5-2) ...
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bioRxiv - Cancer Biology 2019Quote: All tissues were dissociated in 100 mM sodium carbonate using TissueLyzer II (QIAGEN) for 0.5 min at a rate of 0.30 repetitions three times and stored on ice for 30 sec between the dissociation cycles ...
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bioRxiv - Physiology 2020Quote: ... with 0.1% (w/v) sodium dodecyl sulphate (SDS) with a Tissue Ruptor (Qiagen). Samples were centrifuged for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % (w/v) sodium deoxycholate) and once with 500 µl EB buffer (Qiagen). Beads were treated with 300 µg/ml RNase A ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted using a salt extraction protocol (Aljanabi and Martinez 1997) with a RNase A treatment (Qiagen). A total of 1438 flies were successfully genotyped at the Cf-Inv(1 ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitates (IPs) were washed in high salt buffer and RNA from inputs and IPs extracted using the RNeasy micro kit (Qiagen).
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bioRxiv - Bioengineering 2022Quote: ... A high salt solution in combination with isopropanol was utilized for precipitation of RNA before purification with the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
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bioRxiv - Biochemistry 2022Quote: ... complexes were either concentrated using a 50 kDa MWCO concentrator and further purified on a Superose 6 Increase 10/300 column (Cytiva) equilibrated in low-salt CSFxB or loaded directly onto Ni2+ agarose (Qiagen), eluted using 300 mM imidazole ...
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bioRxiv - Immunology 2024Quote: ... The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson, Bioscience, Salt Lake City, UT, USA) using the QuantiTect SYBR Green (Qiagen) and LightCycler® 480 system (Roche Diagnostics ...
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bioRxiv - Cell Biology 2021Quote: ... Sodium bisulfite conversion of unmethylated cytosines in DNA was based on EpiTect Bisulfite Handbook (Qiagen). We designed primers (Table S3 ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNAs were extracted from samples collected at same times from salt-treated or control plant batches using the RNeasy plus mini kit with gDNA eliminator (Qiagen, Germany). First-strand cDNAs were synthesized from 3 μg of RNAs using SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Then the magnetic beads were washed in high salt buffer and RNA was extracted with the RNeasy Micro kit (Qiagen: 74004). For RNA sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... and salt treated samples (100 mM, 250 mM and 500 mM NaCl) was extracted using RNeasy Plant Mini Kit (Qiagen, Germany). RNA quality and quantity assessment ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-bead complexes were washed in high and low salt buffer and RNA was eluted from the RNA-bead complexes using the Qiagen RNeasy Plus Micro Kit (Qiagen 74034). Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230) ...
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bioRxiv - Cell Biology 2021Quote: ... Two μg of DNA was sodium bisulphite modified using the Eptech Kit (Qiagen GmbH, Hilden, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cell pellets were resuspended in 50 mM sodium citrate buffer and treated with RNase A (QIAGEN) for 2 hours at 50°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.1 mM sodium orthovanadate and 0.2 mM phenylmethylsulfonyl fluoride (PMSF)) with a Tissue RuptorTM (Qiagen, Netherlands) homogenizer ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted from tissue and blood in salt solution using a DNeasy Blood and Tissue kit (QIAGEN Pty Ltd, VIC, Australia) or a Gentra Puregene Blood Kit (QIAGEN Pty Ltd ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from the distal half of a walking leg of each lobster using salt extraction (Aljanabi & Martinez, 1997) with an additional RNase treatment following the manufacturer’s instructions (Qiagen Inc., Toronto, Ontario, Canada). DNA quality was assessed using 1% agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μg of RNA was resuspended in 10 μl of RNAse free water and mixed with sodium bisulfite pH 5.0 (42.5 μl) and DNA protection buffer (17.5 μL) (EpiTect Bisulfite Kit, Qiagen). The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... Adapter-ligated DNA was treated with sodium bisulfite using the EpiTect Plus Bisulfite kit (QIAGEN, Hilden, Germany) and amplified using the Kapa HiFi Uracil + ReadyMix (Roche ...
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bioRxiv - Paleontology 2019Quote: ... DNA was then exposed to two rounds of sodium bisulfite treatment using the EpiTect Bisulfite kit (QIAGEN), and paired-end DNA sequencing was performed using the Illumina Hi-Seq 2000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The complex was initially crystallized in a buffer 0.1 M MES pH 6.5 and 0.6 M sodium fluoride from Anions Suite crystallization screen (Qiagen). After optimization of crystallization conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... resuspended in 50 mM sodium citrate buffer containing 10 µl of RNase A (20 mg/ml, Qiagen 76254) and incubated for 2 hours at 50°C ...
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bioRxiv - Neuroscience 2021Quote: Five hundred ng of DNA was treated with sodium bisulfite using the EpiTect Fast DNA Bisulfite kit (Qiagen). Primers for nested PCR (2 fragments for HCRT ...
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bioRxiv - Microbiology 2021Quote: ... 1mM PMSF and 0.25% sodium deoxycholate and the clarified lysate was loaded on a Ni-NTA column (Qiagen). Non-specifically bound proteins were removed by washing with lysis buffer containing 20mM imidazole and the proteins eluted with buffer containing 150mM imidazole ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng of each native library were treated by sodium bisulfite using the EpiTect Bisulfite kit (Qiagen, Valencia, CA) and purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Genomics 2019Quote: ... De-crosslinked DNA was added with 10μl 3M sodium acetate pH 5.2 and purified with QIAquick PCR Purification kit (QIAGEN 28106) according to manufacture instruction ...
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bioRxiv - Plant Biology 2023Quote: ... Adaptor-ligated libraries were subjected to two successive treatments of sodium bisulfite conversion using the EpiTect Bisulfite kit (Qiagen) as outlined in the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Adaptor-ligated libraries were subjected to two successive treatments of sodium bisulfite conversion using the EpiTect Bisulfite kit (Qiagen) as outlined in the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Total cell lysates were generated in RIPA buffer (1% Nonidet P40, 0.5% sodium deoxycholate, 0.1% SDS in PBS) by homogenization with a Tissuelyser TM (QIAGEN, Venlo, Netherlands) at a frequency of 30 Hz for 2 minutes at 4°C ...