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Citations for Qiagen :
1 - 38 of 38 citations for DSPE PEG 2000 Amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 25 %(w/v) PEG 2000 MME from PEGs I suite screening plate (Qiagen). Then ...
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bioRxiv - Microbiology 2019Quote: ... PEG’s and PEG’s II Suites (Qiagen). Diffraction quality crystals of Crp grew from PACT Suite condition H12.
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bioRxiv - Biochemistry 2021Quote: ... 25% PEG 8000 from the PEG suite screen (Qiagen) after 48 hours at 18°C ...
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bioRxiv - Microbiology 2022Quote: ... 20% (w/v) PEG 10000 (Qiagen PEG I, D5). Before harvesting the crystal ...
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bioRxiv - Biophysics 2021Quote: ... PEG suite (Qiagen), PEG Rx (Hampton Research) ...
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bioRxiv - Microbiology 2020Quote: ... 30% PEG 6000 (QIAGEN) at 20 °C using the hanging drop vapor diffusion method ...
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bioRxiv - Microbiology 2020Quote: ... 30% PEG 6000 (QIAGEN) at 20 °C using the hanging drop vapor diffusion method ...
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bioRxiv - Microbiology 2020Quote: ... 30% PEG 3000 (QIAGEN) at 20 °C using the hanging drop vapor diffusion method ...
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bioRxiv - Biochemistry 2023Quote: ... PEG Suite I-II (Qiagen), and Additive Screen kits (Hampton Research ...
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bioRxiv - Immunology 2020Quote: Crystallization conditions were screened using the PEGs II suite (Qiagen) at 20°C ...
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bioRxiv - Microbiology 2022Quote: ... 25% (w/v) PEG 1500 (QIAGEN PACT screen, condition 42).
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bioRxiv - Biochemistry 2020Quote: ... Morpheus (Molecular Dimension, Newmarket, UK) and PEG Suite (Qiagen, Hilden, Germany). Diffracting crystals of the MALX-E-NirF fusion protein were obtained with 30 mg/ml protein and a precipitant containing 20% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Initial conditions were obtained with the PEGs II Suite screen (Qiagen) by vapor diffusion in sitting drops at 18°C using a Mosquito robot (TTP Labtech) ...
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bioRxiv - Biochemistry 2024Quote: ... Initial crystals were obtained via sitting drop vapor diffusion from the PEGs Suite (Qiagen) in 96 well plates and optimized with hanging drop vapor diffusion in 24 well plates ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Biophysics 2019Quote: ... the complete DNA P1P2P3P4P5A was PEG purified and subsequently purified using Qiaquick PCR purification spin columns (Qiagen), the concentration was determined by UV spectrophotometer (Supplementary Fig ...
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bioRxiv - Immunology 2020Quote: ... MuSK1A crystals were grown at 18 °C by vapor diffusion and after ≈ 4 days grew from drops consisting of 100 nL protein plus 100 nL of a reservoir solution consisting of 20% w/v PEG 6K (Precipitant) and 0.1 M BICINE 8.5 pH (Buffer) from the JCSG Core I (Qiagen) screen ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mM GDPNP-Mg2+) were obtained at 4°C using 0.2 M Lithium nitrate and 20% PEG3350 (PEG Suite I, Qiagen) as precipitating agent ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-His tag (1:2000; QIAGEN) and rabbit anti-GST (1:2000 ...
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bioRxiv - Biochemistry 2023Quote: ... anti-RGS-His (Qiagen, 1:2000, 34610), anti-Myc (ChromoTek ...
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bioRxiv - Biochemistry 2022Quote: ... the best diffracting crystal for the 14-3-3σ Clu3/pS197 complex grew in condition G2 (0.2 M NaBr, 100 mM BisTris propane pH 7.5, 20% PEG 3350) of PACT screen (Qiagen, Hilden, Germany). Crystals were mounted in nylon loops directly from crystallization drops and flash frozen in liquid nitrogen without additional cryoprotection ...
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bioRxiv - Immunology 2020Quote: ... Phage particles were purified from the culture media using PEG/NaCl and uridine containing single-stranded DNA (dU-ssDNA) extracted using a QIAprep spin M13 kit (Qiagen). Mutagenesis was carried out by annealing degenerated oligonucleotides to the dU-ssDNA ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into neuronal cultures using Lipofectamine 2000 (Qiagen). Pharmacological LTP was induced in neuronal cell cultures using a 4-hr treatment with 4BF ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with primary antibody (Penta·His Antibody, QIAGEN®, 1:2000 dilution) in 1% bovine serum albumin in PBST for 2h at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... The sample was subsequently homogenized for two cycles at 2000 rpm (PowerLyzer 24, Qiagen). The steel beads were removed ...
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bioRxiv - Biochemistry 2022Quote: ... a 1:2000 dilution of a mouse anti-Penta-His Alexa Fluor 647 conjugate (Qiagen) was used.
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bioRxiv - Developmental Biology 2023Quote: ... the precipitates were subjected to Western blot analysis with anti-penta-His (1:2000, Qiagen) and anti-FLAG M2 (1:4000 ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was extracted from around 2000 worms using Trizol extraction combined with column purification (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Immunology 2020Quote: ... antibodies for 24 hrs before 2000 live cells were FACS sorted directly into lysis buffer (RLT buffer (Qiagen)) ...
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bioRxiv - Physiology 2022Quote: ... RNA (2000 ng) was reverse transcribed to cDNA using the QuantiTect Reverse Transcription Kit (Cat No. 205311, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA (2000 ng) was transcribed into cDNA in a volume of 20 μl using 4 U Omniscript reverse transcriptase (Qiagen). To determine the relative abundance of the mRNAs of interest ...
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bioRxiv - Microbiology 2020Quote: ... the PCR products were loaded on 2% agarose gel and cut from the gel with Tanon UV-2000 gel imaging system and extracted from the agarose using the QIAquick Gel Extraction kit (Qiagen). DNA concentrations were determined using the Qubit™ dsDNA HS Assay Kit on Qubit®2.0.
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was isolated from 2000 cells per replicate and 3 replicates per treatment by the micro RNeasy kit (QIAGEN) for both mouse muscle satellite cells and ZeMPCs ...
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bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was quantitated using Nanodrop ND-2000 and cDNA was synthesized from 500 ng of total RNA using a Quantitect® reverse transcription kit (Qiagen, USA). Gene expression analysis of TET 1 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We extracted and purified genomic DNA from tail tissue either using a sodium hydroxide method (Truett, Heeger et al. 2000) or by proteinase K digestion followed by high-salt precipitation (Gentra/Qiagen, Valencia, CA, USA). We measured the concentration and purity of DNA using a small-volume spectrophotometer (Nanodrop ...