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Citations for Qiagen :
1 - 50 of 10000+ citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from sorted CMP/GMP using the mRNeasy kit (Qiagen, Valencia CA) following manufacturer instructions and quality assessed using Agilent 2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2022Quote: ... hCD45+Lin-CD34+CD38- CD45RA+CD90- and GMP: hCD45+Lin-CD34+CD38+CD45RA+CD123+) was extracted using QIAamp DNA Mini Kit (Qiagen). SNP-CGH array hybridization was performed using the Affymetrix Cytoscan® HD (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... hCD45+Lin-CD34+CD38- CD45RA+CD90- and GMP: hCD45+Lin-CD34+CD38+CD45RA+CD123+) was extracted using QIAamp DNA Mini Kit (Qiagen). Sanger sequencing was performed with forward or reverse primers (TableS6a ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from FACS-sorted L-GMPs from BL/6 and Il21-/- AML mice using the RNeasy Micro Kit (Qiagen). RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen) ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was isolated using the Oligotex direct mRNA kit (Qiagen) according to the manufacturer recommendations ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using the Direct-zol RNA MiniPrep Plus Kit (Qiagen) following manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... we used a direct on-plate lysis of cells with 300 μL of Buffer RLT Plus (Qiagen) supplemented with 1% beta-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Plates contained 5 µl of TCL lysis buffer (Qiagen) supplemented with 1%β-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... direct lysis was performed by adding lysis buffer of the RNeasy Mini Kit (Qiagen) to individual wells ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was isolated using the SampleIN Direct PCR Kit (highQu) or the AllPrep DNA/RNA/Protein Mini Kit (Qiagen), and amplified with ALLin HS Red Taq Mastermix (highQu ...
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bioRxiv - Genomics 2020Quote: ... We extracted mRNA from total RNA with the Oligotex Direct mRNA mini kit (Qiagen 72022).
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bioRxiv - Genetics 2021Quote: ... 5 mM Multiplex-Kit (Qiagen) and HPLC water to a total volume of 10 μL per sample ...
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bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
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bioRxiv - Genetics 2021Quote: Multi-array ELISA was performed using the Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... in 384 well plates in a total volume of 5 μl with 2.5 μl Qiagen Multiplex PCR Plus Kit (Qiagen, Hilden, Germany) and 1 μl Qiagen Q-solution using a Yakima Yellow-labelled primer ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Biochemistry 2020Quote: Cells seeded in 6 well plates were grown in 5% FBS to a confluency of 70% before RNA extraction using the RNEasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... around 25 mg plant powder was used for direct RNA isolation by RNeasy Plant Mini Kit (Qiagen). Polysome-bound RNA or total RNA was subjected to RNA-seq library generation by BGI Genomics following the manufacturer’s BGISEQ-500 protocol.
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 whole-genome sequencing was performed by using QIAseq DIRECT SARS-CoV-2 Kit (QIAGEN). The quality of paired-end reads obtained from MiSeq sequencing was analyzed by using Qiagen CLC Genomics Workbench 22.0.1 and the Identify ARTIC V3 SARS-CoV-2 Low Frequency and Shared Variants (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted by direct cell lysis using the RNeasy Plus Mini Kit with QiaShredder (Qiagen #47134). RNA samples with an RNA integrity number (RIN ...
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bioRxiv - Bioengineering 2023Quote: ... direct RT-qPCRs were performed on heat-inactivated samples without extraction using the QuantiTect Reverse Transcription Kit (Qiagen). The reactions were run in an Applied Biosystems QuantStudio 7 Flex System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: Pathway analysis of differentially expressed genes in direct and indirect or direct only interactions were investigated using Ingenuity Pathway Analysis (IPA) (QIAGEN) (Shannon et al. ...
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bioRxiv - Systems Biology 2019Quote: ... The SWIM plates were purified (Qiagen, PCR Purification Kit) and processed to make an Illumina sequencing library ...
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Hepatic FGF21 mediates tissue tolerance during bacterial inflammation by preserving cardiac functionbioRxiv - Immunology 2020Quote: ... RNA was extracted using the RNeasy or Direct-Zol Kits according to manufacturer’s protocol (Qiagen or Zymo Research, respectively). cDNA synthesis was performed using MMLV reverse transcriptase (Clontech ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were harvested 3 days later by direct application of buffer RLT from the RNeasy mini kit (Qiagen 74104).
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bioRxiv - Cancer Biology 2023Quote: ... organoids were harvested by direct incubation in 350 μl RLT plus lysis buffer (Qiagen RNeasy® Plus Micro kit) in each well for 5 minutes and their RNA was extracted using the Qiagen RNeasy® Plus Micro kit according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2022Quote: ... single cells were sorted into PCR plates containing 5 µl Buffer RLT Plus (Qiagen) with 1% BME and immediately frozen at -80°C for G&T sequencing.
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bioRxiv - Microbiology 2021Quote: Two conventional genomic DNA extraction methods were used in this study for comparison with the direct PCR methods: DNeasy blood and tissue kit (Qiagen) and DNeasy Powersoil kits (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA from cultured cells was isolated with Direct-zol RNA miniprep (Zymogen) and from tissues using miRNeasy Mini kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... or the DNeasy® 96 Blood & Tissue plate kit (Qiagen) according to supplier’s protocol.
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bioRxiv - Genomics 2019Quote: ... phagocytosed by sorting cells into a 96 well plate containing 5 ul of RLT (Qiagen) + 1%β-Mercaptoethanol (Sigma).
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bioRxiv - Immunology 2022Quote: ... single-cells were sorted in 96-well plates containing 5 µL of TCL buffer (Qiagen) with 1% β-mercaptoethanol according to the gating strategy shown in Fig ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of Taq PCR Master Mix kit (Qiagen), 0.4 μl of each primer (100 pmol/μl ...
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bioRxiv - Microbiology 2021Quote: ... NG1 (5’ACCGACCACAGGGGG-3’) and NG2 (5’-GGTTGTAAACCTCTTTCGA-3’) and HotStarTaq® Master Mix Kit (Qiagen) up to a final volume of 25 μL ...
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bioRxiv - Immunology 2023Quote: Total mRNA was isolated from 5×105 cells using a kit (RNeasy kit, QIAGEN) and reverse-transcribed using oligo(dT)20 primer (SuperscriptIII ...
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bioRxiv - Microbiology 2021Quote: ... After 24 hours the media was removed for ELISA and the cells were lysed and RNA extracted (RNeasy Mini Kit, Qiagen).
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bioRxiv - Microbiology 2020Quote: ... 5) Powersoil® Isolation kit (MO Bio Laboratories/Qiagen, Canada) with modifications ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was extracted using the RNeasy 96-well plate kit (Qiagen, #74181) according to manufacturer’s instructions including the DNase step ...
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bioRxiv - Developmental Biology 2023Quote: ... at 300 cells per well into 96 well LoBind plates containing 5 μL of EB buffer (Qiagen). After sorting ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was isolated from Hep3B cell spheroids on the pillar plate and in the ULA plate using a RNeasy Plus kit (Qiagen,74134). One μg of RNA was converted into cDNA using the high-capacity cDNA reverse transcription kit according to the manufacturer instructions (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: Cytokine- or vehicle-stimulated primary CD1 astrocyte cultures in 6-well plates and neuron cultures in 24-well plates were lysed and their total RNA extracted using RNeasy Mini Kits (Qiagen 74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA from 10 adult flies (5 males/5 females) was extracted using DNeasy Blood & Tissue Kit (Qiagen). DNA was resuspended and sheared in 1X TE (0.1 mM EDTA ...
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bioRxiv - Immunology 2020Quote: ... A total of 78 serum samples (positive for HEV IgM in ELISA) were subjected to RNA extraction using QIAamp Viral RNA Mini Kit (Qiagen, Germany). Using SuperScript™ III One-Step RT-PCR System with Platinum™ Taq (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... and 5 cells per well were sorted into a 96-well plate containing TCL buffer (Qiagen, cat. 1031576) with 1 % beta- Mercaptethanol and snap frozen on dry ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Plates were then placed at 4 0C before adding the reverse transcription mix containing 5’-biotinylated TSO (Qiagen). PCR products were cleaned up using 0.8:1 Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: RNA was extracted from 6-well plates using miRNeasy Mini Kits (QIAGEN 217004) with the inclusion of an on-column DNase digestion (QIAGEN 79254) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted directly from the plates using RNeasy Mini Kit (Qiagen, 74104). 2μg of RNA were used for cDNA synthesis using High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Direct quantification data were acquired with the QIAcuity Software Suite 2.2.0.26 (Qiagen) and chlamydial 16S rRNA copy numbers per mL were calculated based on these data ...
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bioRxiv - Systems Biology 2020Quote: ... IL-8 ELISA was performed according to manufacturer instructions (Qiagen).
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bioRxiv - Molecular Biology 2021Quote: ... Oligonucleotides containing shRNA inserts were PCR-amplified with primers 5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’ and 5’-TGAACTCGAGAAGGTATATTGCTGTTGACAGTGAGCG-3’ and purified with QIAquick PCR Purification Kit (Qiagen). shRNA inserts and miRE18_LT3GEPIR_Ren714 backbone (inducible via Tet-On system ...