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Citations for Qiagen :
1 - 7 of 7 citations for Clostridium Difficile Toxin A Ribotype 027 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... difficile cultures using DNeasy Blood and Tissue Kit (Qiagen). PCR reactions were carried out using gene specific primers (Table S2) ...
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bioRxiv - Microbiology 2020Quote: ... difficile were performed using the Rotor-Gene system 6200 (Qiagen, Tokyo, Japan), as previously described [29] ...
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bioRxiv - Microbiology 2021Quote: ... difficile strain 630 genomic DNA and cloned into BamHI and PstI sites of pQE30 expression vector (Qiagen). All pQE30-derived plasmids were initially transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... Toxins were then purified from bacterial cell lysates through affinity chromatography on a Nickel-resin (Qiagen), ion exchange ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the polyhistidine-tagged toxin was loaded onto a Ni-NTA agarose column (Qiagen). The column was then washed with a 20–120 mM gradient of imidazole to remove additional contaminating proteins ...
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bioRxiv - Microbiology 2020Quote: RNA was harvested from AKI toxin-inducing culture pellets preserved in 1mL Trizol using an RNEasy kit (Qiagen) using both on-column DNase digestion (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... pH was adjusted to 8 after resuspension to allow the binding of 6x His-tagged toxins to Ni-NTA agarose resin (reference: 30210; Qiagen). Resulting samples were passed through chromatography columns containing the Ni-NTA agarose resin and were eluted with denaturing buffer containing increasing concentrations of Imidazole (10 mM ...