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Citations for Qiagen :
1 - 50 of 826 citations for Charged Multivesicular Body Protein 2b CHMP2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and the recombinant protein was purified by gravity-flow chromatography using a nickel-charged resin Ni-NTA Agarose (QIAgen) equilibrated with 10 mM imidazole in the lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL of nickel-charged resin (Qiagen, Ni-NTA Agarose) was added to a column (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified lysate was incubated on charged Nickel beads (Qiagen) that have been pre-equilibrated in lysis buffer containing 1% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Body parts were collected in microtubes (Qiagen) containing 5 mm diameter stainless steel beads (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... melanogaster whole body tissue (Qiagen RNeasy Mini Kit).
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bioRxiv - Immunology 2022Quote: ... BEAS-2B and MRC-5 (2×106 cells/well) using the RNeasy Mini Kit (Qiagen) with DNase I treatment to eliminate DNA contaminants as previously described18 ...
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bioRxiv - Physiology 2019Quote: ... the inclusion bodies loaded onto Ni-NTA Superflow (Qiagen, Valencia, CA, USA) were subjected to a protein sequencer (Procise 491HT ...
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bioRxiv - Biochemistry 2022Quote: ... coli as described in (Péli-Gulli et al., 2015) using Ni-charged agarose beads (QIAGEN, product number 30210). Proteins were eluted in elution buffer (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Synthetic Biology 2022Quote: ... the supernatant was purified by Ni2+ immobilized metal affinity chromatography (IMAC) with Ni-NTA charged magnetic beads (Qiagen). Magnetic beads with bound cell lysate were washed with 200 μL of washing buffer for three times (150 mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... abdomen carcasses with adhering fat body were homogenized in ATL buffer (QIAGEN 19076) containing 0.4 mg Proteinase K (Sigma Aldrich P8044) ...
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bioRxiv - Physiology 2023Quote: ... or from 10 dissected fat bodies using the QIAGEN RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was filtered with a 0.45 μm filter and loaded onto a nickel-charged immobilized metal affinity chromatography column (Qiagen). After washing with ten column volumes of buffer A ...
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bioRxiv - Immunology 2022Quote: Total RNA from whole-body killifish samples was isolated using QIAzol lysis reagent (QIAGEN, 1 ml of reagent per 0.1 g of homogenized tissue ...
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bioRxiv - Microbiology 2022Quote: ... then leg and body samples were homogenized using a TissueLyser II (Qiagen, Hilden, Germany) at 30 Hz for 2 min ...
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bioRxiv - Genetics 2019Quote: Genomic DNA of the preserved bodies was extracted using DNeasy Blood & Tissue Kit (Qiagen), with an additional RNase digestion for removing RNA from the extracted nucleic acid samples ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was isolated from preserved bodies using the DNeasy Blood and Tissue Kit (Qiagen). Separate Illumina paired-end libraries were generated according to the manufacturer’s protocol (Illumina Inc.) ...
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bioRxiv - Molecular Biology 2023Quote: ... pooled insects whole bodies or tissues were homogenized using the Tissue Lyser II (Qiagen) in a 1.7 ml microcentrifuge tube containing 500 µl of ice-cold trizol and five three-millimeter glass beads (Pyrex) ...
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bioRxiv - Molecular Biology 2021Quote: ... recombinant CLEC16A and CLEC16A ΔC were generated following expression in 293T cells and purified with nickel-charged resin (Ni-NTA agarose; Qiagen) per the manufacturer’s protocols.
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bioRxiv - Evolutionary Biology 2022Quote: ... 2022 for sampling) was extracted from fat body using DNeasy Blood and Tissue kit (Qiagen), including RNase A treatment (Qiagen) ...
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bioRxiv - Genetics 2023Quote: ... Mosquito heads or bodies were grinded in a 96 wells plate using a TissueLyser (Qiagen) for 2 min at 30 oscillation/s ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified supernatants containing TtgR and SmtB were purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The eluted fractions from the FPLC (for TetR ...
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bioRxiv - Synthetic Biology 2023Quote: ... Clarified supernatant containing mRFP1 was then purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The elution from the gravity column was concentrated and buffer exchanged (25 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from whole mosquito bodies using the DNeasy Blood and Tissue kit (Qiagen, UK). PCR was performed for each individual mosquito ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from 10 homogenized bodies using the RNeasy Minikit with DNase treatment (Qiagen) and reverse transcription was performed using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: DNA extraction of mosquito bodies was performed using the QIAmp DNA Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was analyzed by immunoblotting using monoclonal anti-RGSH4 antibodies (Qiagen) to detect ABCG5 and polyclonal anti-hABCG8 antibodies (Novus Biologicals ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The fat bodies then were disrupted using a TissueLyser LT small beads mill (QIAGEN K.K., Tokyo, Japan). These disrupted samples were preserved at −80°C until used ...
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bioRxiv - Molecular Biology 2019Quote: Body tissue of medaka at 3 dph was minced and then processed with a RNeasy Minikit (Qiagen) for extraction of total RNA ...
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bioRxiv - Microbiology 2020Quote: ... or head and bodies separately for blood-fed mosquitoes) using the RNeasy 96 QIAcube HT kit (Qiagen). DENV-3 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The fat bodies then were disrupted using a TissueLyser LT small beads mill (QIAGEN K.K., Tokyo, Japan). These disrupted samples were preserved at −80°C until used ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were blotted onto PVDF membrane and examined with an RGS-His4-antibody (Qiagen) and a 1:2500 dilution of the fluorophore-conjugated secondary antibody Cy3 (ECL Plex goat-α-mouse IgG-Cy3 ...
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bioRxiv - Microbiology 2021Quote: ... tularensis IM protein SecY (Huntley, 2007) or the Penta-His HRP conjugate antibody (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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bioRxiv - Genetics 2019Quote: ... and then subjected to caudal fin biopsy for genotyping and the body stored in Qiazol (Qiagen. Cat#: 79306) at 7 dpf ...
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bioRxiv - Genomics 2019Quote: ... DNA extraction was performed using the entire body of several workers using DNeasy Blood and Tissue kits (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: We extracted total RNA from either gut or body tissues using the RNeasy RNA mini extraction kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... was expressed in Rosetta2(DE3)pLysS E.coli competent cells and purified from inclusion bodies by affinity chromatography using Ni2+-nitrilotriacetic acid Superflow resin (QIAGEN). Recombinant hamster PrP (HaPrP ...
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bioRxiv - Bioengineering 2023Quote: Each body part (legs and thorax) was homogenized individually for 3 × 1 minute at 30 Hertz using TissueLyser (Qiagen) and glass beads (#11079110 ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted from homogenized whole bodies with a Qiagen DNeasy kit after overnight treatment with 10% proteinase K (Qiagen) at 56 °C ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from dissociated DRG neurons or cell body/axon compartments collected from insert cultures using RNeasy micro isolation kit (Qiagen). Purified RNAs were quantified with Ribogreen (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from dissociated DRG neurons or cell body/axon compartments collected from insert cultures using RNeasy Microisolation kit (Qiagen). Sciatic nerve was cut in small pieces and digested with collagenase at 37°C for 30 min with intermittent trituration ...
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bioRxiv - Biochemistry 2022Quote: ... Animals were washed three times with M9 buffer and total RNA from their bodies (excluding embryos) was extracted using the RNeasy kit (Qiagen), with an additional step of on-column DNase I (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... day 10 embryoid bodies (EBs) and D45-47 cortical organoids (3 pooled EBs or COs per sample) using RNeasy Mini kit (Qiagen). Approximately 0.5-1ug of RNA was used as input to generate cDNA using Superscript IV Reverse Transcriptase (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... These individuals were at different larval stages and total RNA was extracted from whole bodies using an ALLPrep DNA/RNA Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA was extracted from whole bodies of 20-25 individuals using Trizol (Signma) followed by clean-up and on-column DNAse digestion using RNeasy (Qiagen) according to the manufactures’ protocols ...
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bioRxiv - Microbiology 2022Quote: ... was extracted from isolated adult bee guts or whole-body for larva using a QIAamp PowerFecal Pro DNA Kit (Qiagen). Gut tract from each surface-sterilized adult bee was dissected in sterilized phosphate buffered solution (PBS ...
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bioRxiv - Genomics 2023Quote: For bisulfite treatment of a single polar body, we followed the Wei protocol (Wei et al., 2019) and used the EpiTec Fast LyseAll Bisulfite Kit (Qiagen) with all volumes quartered ...
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bioRxiv - Evolutionary Biology 2023Quote: The abdomen of each adult moth was separated from the body and used for DNA extraction with the Qiagen DNeasy Blood & Tissue kit (Qiagen, Germantown ...