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Citations for Qiagen :
1 - 50 of 139 citations for CD8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-His6 (34650; IF: 1/500) from Qiagen, mAb anti-GAPDH (GTX28245 ...
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bioRxiv - Immunology 2021Quote: ... and an undisclosed peptide pool inducing CD8+ T lymphocyte stimulation (Qiagen, 2017); rmsHBHA which tubes contain recombinant M ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from isolated CD8+ T cells (RNeasy kit, Qiagen) and 300 ng of RNA were used for cDNA synthesis (First-Strand Synthesis kit ...
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bioRxiv - Immunology 2022Quote: ... Recombinant plasmids were then isolated (MiniPrep, Qiagen) and sequenced (DNA Sequencing Facility ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 647-conjugated Penta-His mAb was purchased from Qiagen, Germantown ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was treated with Penta-His MAb (Qiagen, Germantown, MD) followed by HRP-conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... total RNA of CD8+ human T cells was extracted by miRNeasy Mini Kit (Qiagen), following the manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... DNA for library preparation was extracted from CD8+ T-cells using FlexiGene DNA Kit (Qiagen). 150 ng aliquots of obtained DNA were used as input to prepare each out of four TRA libraries ...
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bioRxiv - Immunology 2022Quote: ... CD4+/CD8+ T cells and Tfh from indicated organs using RNeasy Mini Kit (Qiagen Inc.). cDNA synthesis was performed using Superscript III First Strand Synthesis System (Invitrogen).
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bioRxiv - Biophysics 2019Quote: ... Anti-tetra-His-tag mouse monoclonal antibody (mAb) IgG1 was purchased from Qiagen Com ...
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bioRxiv - Plant Biology 2021Quote: ... recombinant protein and purified using Ni-NTA resin (Qiagen). Polyclonal antibodies were raised in mice as described in 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant protein was purified using Ni-NTA agarose (Qiagen). Rabbit polyclonal antibodies were generated by Covance ...
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bioRxiv - Immunology 2020Quote: DNA and RNA were extracted simultaneously from isolated CD8+ T-cell samples using AllPrep MiniKit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from naïve CD8 T cells using the RNeasy Micro Kit (cat# 74004, Qiagen). Naïve T cells were isolated from four female control (Ptbp1fl/fl ...
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bioRxiv - Immunology 2023Quote: Total RNA was purified from isolated CD8+ T cell subsets using the RNeasy Mini Kit (Qiagen), spectrometrically quantified (DeNovix) ...
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bioRxiv - Developmental Biology 2020Quote: ... recombinant proteins were purified using Ni-NTA Agarose (Qiagen 30210). Buffer was exchanged by dialysis to PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen) according to standard protocols (21) ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant proteins were then purified by Ni-NTA-agarose (Qiagen) affinity chromatography ...
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bioRxiv - Biophysics 2023Quote: ... The recombinant proteins were purified using nickel affinity chromatography (Qiagen). As the final purification step ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA from human primary CD4+/CD8+ T cells was isolated using the Gentra Puregene Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: CD4+ and CD8+ T cells were collected on the FACS Aria Fusion into RNAse free eppendorfs (Qiagen), transported on ice and cell pellets were resuspended and lysed in RLT buffer (Qiagen ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from FACS/MACS-isolated CD8+ T cells and reverse-transcribed using RNeasy Micro Kit (Qiagen) and SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... CD3+CD8+CD45RA- and CD3+CD4+CD45RA-cell populations were bulk sorted then frozen into RLT buffer (Qiagen).
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from 0.5×106 naïve and activated CD8+ T cells using a using an RNeasy Mini kit (Qiagen) and additionally purified using an RNase Free DNase Set (Qiagen ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... and non-MAIT T cells (mMR1-tet-CD4+CD8+TCRβ+ cells) were sort-purified from thymocytes of Vα19 and Vβ8 mice and stored into RNAlater (Qiagen). Total RNA was isolated using RNeasy Mini kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified with Ni-NTA chromatography (Ni2+-nitrilotriacetate, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant plasmids were purified with EndoFree Plasmid Maxi Kit (12362, QIAGEN). Oligonucleotide sequences and further details are provided in Supplementary Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... recombinant protein was initially purified by nickel affinity chromatography (Qiagen, Holland) and subsequent size exclusion using Superdex 200 increase (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant 6xHis-Uhrf1 was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 40,920 cm-1 M-1) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant 6xHis-Swi6 was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 45,330 cm-1 M-1) ...
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bioRxiv - Immunology 2020Quote: ... Recombinant HCMVs were reconstituted from HCMV BAC DNA by Superfect (Qiagen) transfection into permissive MRC-5 cells.
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Ni2+-nitrilotriacetic acid agarose beads (Qiagen) and desalted with Bio-Gel P-6DG gel (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells from PBMCs and colon were FACS sorted directly into 50 μL of RLT Lysis Buffer (QIAGEN) supplemented with 1% v/v 2-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was purified by Ni-NTA affinity chromatography column (QIAGEN).
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bioRxiv - Microbiology 2019Quote: ... Recombinant protein in the supernatant was purified via Ni2+-NTA (Qiagen, UK) column [20].
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were purified from lysates on Ni-NTA columns (Qiagen).
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were purified over nickel-nitrilotriacetic acid (Ni-NTA) Agarose (Qiagen). Therefore ...
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bioRxiv - Bioengineering 2023Quote: ... Recombinant plasmids were prepared by using the QIAprep Spin Miniprep Kit (QIAGEN) and confirmed by sequencing analysis ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plates were coated with 100 μL of 4 μg/mL murine anti-His mAb (Qiagen, #34660), and after blocking ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from circulating PD-1+ and PD-1− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing.
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bioRxiv - Immunology 2021Quote: ... DNA was isolated from sorted IFNγ+ and IFNγ− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from CD8+-depleted PBMCs ex vivo culture supernatants using the QIA amp Viral RNA Mini kit (Qiagen). HIV-1 RNA levels were quantified using the Generic HIV Viral Charge kit (Biocentric ...
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bioRxiv - Microbiology 2021Quote: Total cellular DNA was extracted from patient CD8+-depleted PBMCs ex vivo cultures using the QIAamp DNA Mini kit (Qiagen). The total cell-associated HIV-1 DNA was then quantified by ultra- sensitive real-time PCR (Generic HIV DNA cell kit ...
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bioRxiv - Immunology 2019Quote: ... genomic DNA purified from CD8 T cells and bisulfite converted using the EpiTect Bisulfite Kit as per the manufacturer’s instructions (Qiagen, Inc.). Bisulfite-converted DNA was PCR amplified and cloned with the TOPO TA cloning kit (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: ... RNA was extracted from CD117− CD25− linneg (DN4) and CD4+CD8+ (DP) thymocytes using the RNeasy Plus Mini Kit (Qiagen) and SuperScript lll reverse transcriptase was used to make cDNA using the protocol supplied by Invitrogen (cat # 18080-093) ...
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bioRxiv - Immunology 2019Quote: ... Primary mouse CD4+ T cells were purified from lymph nodes and spleens by negative selection using anti-MHCII and anti-CD8 hybridoma supernatants (M5/114 and 2.43, respectively) and anti-rat Ig magnetic beads (Qiagen BioMag). The resulting CD4+ T cells were then immediately activated on 24-well plates coated with anti-CD3 and anti-CD28 (1 ug/ml each ...
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bioRxiv - Cancer Biology 2023Quote: ... deep sequencing genomic DNA was isolated from PBMC or CD4+ and CD8+ sorted T cell populations following the DNeasy Blood and Tissue Kit (Qiagen) protocol ...
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bioRxiv - Immunology 2023Quote: Genomic DNA from ex vivo isolated CD8+ T cell subsets or in vitro cultured cells was extracted using the DNeasy Blood & Tissue Kits (Qiagen) and subsequently bisulfite-converted by using the EZ DNA Methylation-Lightning Kit (Zymo Research ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) and elution with imidazole ...