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Citations for Qiagen :
1 - 14 of 14 citations for Bordetella Pertussis Toxin Glycerol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... pertussis isolates were extracted using QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany). Whole genome sequencing (WGS ...
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bioRxiv - Immunology 2020Quote: ... and 15% Glycerol from the JCSG Core II (Qiagen) screen ...
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bioRxiv - Cell Biology 2021Quote: ... 10% glycerol) and batch bound to Ni-NTA beads (#30230, Qiagen) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Toxins were then purified from bacterial cell lysates through affinity chromatography on a Nickel-resin (Qiagen), ion exchange ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the polyhistidine-tagged toxin was loaded onto a Ni-NTA agarose column (Qiagen). The column was then washed with a 20–120 mM gradient of imidazole to remove additional contaminating proteins ...
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bioRxiv - Cancer Biology 2021Quote: ... bacterial glycerol stocks were grown and purified using EndoFree Plasmid Kits (Qiagen). Lentiviral production was performed co-transfecting 293-FT packaging cells with individual pLKO_shETS plasmids ...
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bioRxiv - Microbiology 2020Quote: RNA was harvested from AKI toxin-inducing culture pellets preserved in 1mL Trizol using an RNEasy kit (Qiagen) using both on-column DNase digestion (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... pH was adjusted to 8 after resuspension to allow the binding of 6x His-tagged toxins to Ni-NTA agarose resin (reference: 30210; Qiagen). Resulting samples were passed through chromatography columns containing the Ni-NTA agarose resin and were eluted with denaturing buffer containing increasing concentrations of Imidazole (10 mM ...
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bioRxiv - Microbiology 2020Quote: ... crystals were flash frozen (100 °K) in the above reservoir solution supplemented with 10% glycerol (QIAGEN). The diffraction data set for complexes of Brd4(BD1 ...
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bioRxiv - Microbiology 2021Quote: ... 300mM NaCl and 5% glycerol and the clarified lysate was loaded on a Ni-NTA column (Qiagen). Non-specifically bound proteins were removed by washing with lysis buffer containing 40mM imidazole and σA eluted with 150mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1 mM DTT) and recombinant TrcP was purified from clarified lysates using Ni-NTA resin (Qiagen) and gravity chromatography ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 % (v/v) glycerol) for 30 s at a frequency of 15 s-1 using a TissueLyser II (QIAGEN). As the tissue homogenates were to be used for both native and SDS PAGE ...
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bioRxiv - Genetics 2023Quote: ... and the collected pellets were cultured for an additional 12 hours in LB with carbenicillin before preparing glycerol stocks and maxi preps (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... Resuspend the cells in 10 ml of suspension buffer (10 mM EDTA pH8.0, 150 mM NaCl, 1% glycerol, Lysis blue (1×, from QIAGEN Plasmid Plus Midi Kit), RNase A (0.55 mg/ml) ...