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Citations for Qiagen :
1 - 50 of 1937 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
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bioRxiv - Biochemistry 2020Quote: ... Released affinity tags containing Flag-EGFP were removed from the mixture by a Ni-NTA resin (QIAGEN). The non-absorbed fractions were concentrated and subjected to a size-exclusion column chromatograph using a Superose6 Increase column (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... The affinity purification was performed using Ni-NTA affinity column (Qiagen, Venlo, Netherlands), and the purified CyaY protein was then placed in the dialysis buffer and treated by Ulp1 to remove the N-terminal hexahistidine-Sumo tag ...
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bioRxiv - Genomics 2022Quote: ... the supernatants were mixed with Sigma HIS-Select Nickel Affinity Gel (at a ratio of 3:1) equilibrated in lysis buffer before being added into a polypropylene column (Qiagen). After extensive washing with a cold lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... the proteins were purified by affinity chromatography with Ni-NTA superflow affinity column (Qiagen) as previously described (33).
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bioRxiv - Microbiology 2020Quote: ... using an affinity column (Qiagen). The protein purity was monitored by SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... Following Ni-NTA affinity chromatography (Qiagen), the NELF containing protein fractions were pooled ...
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bioRxiv - Biochemistry 2020Quote: ... Nickel affinity chromatography resin (NiNTA, Qiagen) was used to purify HNL1 containing a 6-His tag expressed in Escherichia coli ...
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Structural Basis for SARS-CoV-2 Envelope Protein in Recognition of Human Cell Junction Protein PALS1bioRxiv - Biochemistry 2021Quote: ... the supernatant was collected for affinity purification by nickel-nitrilotriacetic acid affinity chromatography (Ni-NTA, Superflow, Qiagen). The eluate was concentrated and buffer exchanged for tag removal by incubation with TEV protease overnight at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Ni-NTA affinity columns (Qiagen, Hilden, Germany) were used in purification of Nbs ...
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bioRxiv - Cell Biology 2021Quote: ... and nickel affinity resin (Qiagen, Valencia, CA), respectively ...
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bioRxiv - Plant Biology 2023Quote: ... or Ni-NTA affinity agarose beads (QIAGEN). Purified proteins were dissolved in a buffer containing 25 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression and affinity purification using immobilized metal affinity chromatography (IMAC) were carried out according to manufacture’s instruction (Ni-NTA agarose, Qiagen). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Flag-SHOC2 was cleaved from 6X-His-MBP by incubating 37 mg purified protein with 0.65 mg TEV protease at 4°C overnight followed by affinity purification using Nickel affinity resin (Qiagen) where cleaved Flag-SHOC2 was collected in the flow-through.
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 20,000 rpm for 1 hour at 4°C and the soluble fraction was affinity purified by gravity column with Ni-NTA affinity resin (QIAGEN). The protein was eluted by 50 mM Tris pH 8 ...
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bioRxiv - Immunology 2021Quote: ... and Ni-NTA affinity columns were from Qiagen, Hilden ...
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bioRxiv - Molecular Biology 2022Quote: ... A combination of nickel affinity chromatography (Qiagen 30210) and cation exchange (GE Healthcare HiTrap-SP ...
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bioRxiv - Immunology 2022Quote: ... Affinity chromatography with Ni-NTA agarose beads (Qiagen) was used to purify the proteins ...
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bioRxiv - Biochemistry 2023Quote: ... ATG9A was purified by Nickel affinity resin (QIAGEN) followed by Strep-Tactin and gel filtration with Superdex 200 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with NiNTA agarose (Qiagen). The eluate was further purified over a Source 15 Q column (Cytiva) ...
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bioRxiv - Bioengineering 2024Quote: ... before purification by Ni-NTA affinity chromatography (Qiagen) as previously described (Fierer et al. ...
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bioRxiv - Plant Biology 2020Quote: ... metal chelate affinity chromatography on Ni-NTA Agarose (Qiagen) was performed according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... Crude lysates were affinity purified using Nickel resin (Qiagen), followed by cation exchange chromatography using a Mono S column (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... and subjected to Ni-NTA based affinity chromatography (QIAGEN). Eluted protein was further purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... followed by affinity chromatography using Ni-NTA resin (QIAGEN) and size exclusion chromatography using a HiLoad Superdex 200 pg column (Cytiva) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were purified with Ni-NTA affinity resin (Qiagen). The aminoacylation assay protocol from Jiongming Lu was then followed (Lu et al. ...
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bioRxiv - Microbiology 2020Quote: ... coli by affinity chromatography using Ni-NTA agarose (Qiagen) according to the manufacturer’s instructions (Ruc-His6 was purified under urea denaturing conditions) ...
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bioRxiv - Microbiology 2022Quote: ... and purified by metal-affinity chromatography (Ni-NTA, Qiagen). The protein expression was induced by adding 0.4 mM IPTG in log-phase cultures at O.D600 of 0.4 ...
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bioRxiv - Plant Biology 2019Quote: ... and Ni-NTA affinity agarose beads (QIAGEN, Venla, Netherlands) respectively ...
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bioRxiv - Biochemistry 2020Quote: ... and purified by Ni-NTA– agarose affinity chromatography (Qiagen) as described previously (15) ...
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bioRxiv - Microbiology 2021Quote: ... followed by affinity chromatography using Ni-NTA resin (QIAGEN) and size exclusion chromatography using a HiLoad Superdex 200 pg column (Cytiva) ...
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bioRxiv - Synthetic Biology 2023Quote: ... purified by non-denaturing Ni-NTA affinity chromatography (Qiagen), and buffer exchanged into PBS by overnight dialysis through regenerated cellulose tubing (6-8 kDa MWCO ...
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bioRxiv - Biochemistry 2023Quote: ... protein was captured using Ni-NTA affinity chromatography (Qiagen). Protein was further purified using ion exchange chromatography preceding size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... TAT-SAPIP was purified by Nickel affinity chromatography (Qiagen) following the protocols from the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... the lysate was subjected to Ni2+-NTA affinity chromatography (Qiagen) followed by size exclusion chromatography using a Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were purified by Ni2+-affinity (Ni-NTA agarose, Qiagen) then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Biochemistry 2020Quote: ... Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen), the 6 x His-tag was removed via incubation with thrombin protease overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Protein was affinity-purified using Ni-NTA agarose (Qiagen #301210), and exchanged into severing buffer I (SBI ...
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bioRxiv - Biochemistry 2020Quote: ... NT-hFMRP was purified using Ni-NTA affinity chromatography (Qiagen). GST-hFMRP RGG ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified using Ni-NTA affinity columns (Qiagen) and subsequently purified by gel filtration using a HiLoad Superdex 75 pg column (GE) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The supernatants were purified using an affinity Ni2+ column (Qiagen). All proteins were eluted by imidazole of 8 mM ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen) according to standard protocols (21) ...
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bioRxiv - Microbiology 2022Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Biochemistry 2022Quote: ... It was then purified by Ni-NTA affinity chromatography (Qiagen)(50mM Tris pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... and further purified with affinity column (RNeasy Mini Kit, Qiagen) following the manufacturer’s protocols ...
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bioRxiv - Immunology 2019Quote: ... Proteins were purified by affinity chromatography on NiNTA agarose (Qiagen), followed by FPLC on MonoQ (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni-NTA affinity chromatography (Qiagen) and eluted with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Bioengineering 2019Quote: ... Immobilized metal affinity chromatography (IMAC) using Ni-NTA matrix (Qiagen) was employed for purification purposes ...
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bioRxiv - Cell Biology 2020Quote: ... 2ml of packed Ni-NTA affinity chromatography resin (Qiagen, USA) was calibrated with 10 volumes of lysis buffer ...