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Citations for Qiagen :
1 - 50 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... SHP1 and SAP were purchased from QIAGEN (Hilden, Germany) (siSLAMF6(1 ...
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bioRxiv - Plant Biology 2023Quote: ... Mesophyll sap was rapidly collected and deposited into RLT lysis buffer for RNA extraction (RNeasy Plant Mini Kit, Qiagen). Excess moisture was removed of the purified bundle sheath strands on a bed of paper towel ...
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bioRxiv - Immunology 2019Quote: ... Primary mouse CD4+ T cells were purified from lymph nodes and spleens by negative selection using anti-MHCII and anti-CD8 hybridoma supernatants (M5/114 and 2.43, respectively) and anti-rat Ig magnetic beads (Qiagen BioMag). The resulting CD4+ T cells were then immediately activated on 24-well plates coated with anti-CD3 and anti-CD28 (1 ug/ml each ...
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bioRxiv - Immunology 2024Quote: ... Immature (CD24high) thymocytes from both B6 and B6.SAP-/- mice were sorted directly into 350 microliters of RLT lysis buffer (Qiagen). RNA was extracted using the RNeasy Micro kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... and 6×His-Flag-Ub was conducted using Ni-NTA agarose (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used.
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bioRxiv - Biochemistry 2022Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... S2 cells were transiently transfected with pAc-brm-FLAG-6xHIS using Effectene (Qiagen, Valencia, CA). Cells were harvested 48 hours after transfection and proteins were extracted using EB2 buffer (20mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... UAS-CG9253(porthos)::FLAG::HA and srpHemo-GAL4 constructs using Effectene Tranfection Reagent (Qiagen, Hilden, Germany) following the manufacturer’s protocol (Table S3) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting pMT-ball-FLAG vector was transfected into S2 cells using Effectene transfection reagent (Qiagen) and stable cell line was generated by following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Released affinity tags containing Flag-EGFP were removed from the mixture by a Ni-NTA resin (QIAGEN). The non-absorbed fractions were concentrated and subjected to a size-exclusion column chromatograph using a Superose6 Increase column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... or Renilla-HA-NOD2 (750 ng/well) and His-FLAG-CAD (250 ng/well) using Effectene Transfection Reagent (Qiagen). The following day ...
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bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
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bioRxiv - Biochemistry 2021Quote: The plasmids pIB-spike-V5 with or without pIB-GALNTs-FLAG were transfected to S2R+ cells (DGRC) using Effectene transfection reagent (Qiagen) according to the instructions ...
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bioRxiv - Microbiology 2020Quote: ... construct pcDNA6B-nCoV-NSP12-FLAG encoding the RDRP gene (gift from Dr. George Stark, Cleveland Clinic) was digested with Xho1 and purified by Qiagen QIAquick PCR Purification Kit to be used as a standard in the RT-qPCR reaction ...
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bioRxiv - Molecular Biology 2022Quote: Drosophila S2 cells were transiently transfected with pAc-brm-FLAG-6xHIS in combination with either pAc-clk-V5-HIS or pAc-V5-HIS empty plasmid using Effectene (Qiagen). For cycloheximide experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transiently transfected with pAc-brm-FLAG-6xHIS in combination with pAc-clk-V5-HIS or pAc-V5-HIS empty plasmid using Effectene (Qiagen). Cells were harvested 48 hours after transfection for processing ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with FLAG-TRAF7WD40 and Tri1-Strep using Continuum transfection reagent (Gemini Bio) or Effectene transfection reagent (Qiagen). For control conditions ...
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bioRxiv - Microbiology 2023Quote: ... 293A cells were transfected with the plasmid of pCDNA3.1-ACE2 (human)-3×FLAG (MiaoLing Plasmid Platform, China) using polyfect (QIAGEN, West Sussex, UK) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Expression levels were validated from the pWB plasmid by western blot using an anti-His HRP conjugate kit (Qiagen) (Fig ...
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bioRxiv - Neuroscience 2022Quote: ... anti-sense DIG-labeled RNA probes were generated by in vitro transcription and purified with the RNeasy kit (Qiagen). At 55-62°C ...
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bioRxiv - Genomics 2019Quote: The RNA extraction kit (RNeasy Mini Kit, Qiagen) was used for the total RNA isolation ...
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bioRxiv - Bioengineering 2020Quote: ... An RNA extraction kit (RNeasy extraction Kit, Qiagen) was used to purify RNA ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription kits (RT2 First Strand Kit; Qiagen) were used for cDNA synthesis ...
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bioRxiv - Microbiology 2019Quote: ... kit (Qiagen). Phylogenetic groups were determined as described in (Clermont ...
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bioRxiv - Microbiology 2019Quote: ... Kit (QIAGEN) followed with 16S-ITS PCR as previously described (64 ...
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bioRxiv - Genetics 2020Quote: ... Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Kit (Qiagen). Whole-genome sequencing was performed using the Illumina Nextera XT library protocols and sequenced at 2 x 300bp read length on the MiSeq platform (Ramaciotti Centre for Functional Genomics ...
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bioRxiv - Microbiology 2024Quote: ... Kit (Qiagen) using 5 mL culture of V_227 which was grown in MMT-YE medium (20°C ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked for 1 hour at room temperature either with a commercial blocking buffer (QIAGEN, anti RGS HIS6 HRP conjugate kit) or with 5% milk in 0.05% tween TBS buffer ...
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bioRxiv - Bioengineering 2020Quote: ... the membrane was incubated with freshly prepared primary antibody solution (1:1,000 dilution for anti-Alix [ab117600, Abcam], anti-Tsg101 [ab30871, Abcam], anti-Calnexin [ab22595, Abcam], anti-His [34660, Qiagen] ...
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bioRxiv - Molecular Biology 2021Quote: ... The kits for RNA extraction were from Qiagen (RNeasy Mini Kit and RNeasy Micro Kit). The reverse transcription kit ...
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bioRxiv - Neuroscience 2021Quote: ... Using a commercial kit (QIAGEN DNeasy isolation kit #69506), DNA was isolated from a tissue punch taken from the external ear ...
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bioRxiv - Microbiology 2023Quote: ... using a commercial kit (RT2 First Strand Kit, Qiagen). Quantification of transduction of EBECs and HBECs with the SARS-CoV-2 pseudovirus was determined by real-time qPCR (rt-PCR ...
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bioRxiv - Microbiology 2023Quote: ... and the RNeasy kit plus Mini kit (#74134, Qiagen) or using NucleoSpin RNA Plus XS ...
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bioRxiv - Genetics 2019Quote: ... DNA maxiprep kit was from Qiagen (EndoFree Plasmid Maxi Kit). All plasmids were cloned with Gibson assembly (42 ...
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bioRxiv - Cell Biology 2021Quote: ... QIAquick PCR purification kit or QIAquick Gel Extraction kit (Qiagen). Cloning strategies for each construct are described in Table S1 ...
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bioRxiv - Genetics 2022Quote: ... we used the QIAGEN kit (DNeasy Blood & Tissue Kit; Qiagen, https://www.qiagen.com/ ...
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bioRxiv - Neuroscience 2024Quote: ... QIAfilter Plasmid kits (Midi prep kit; Qiagen; catalog no.: 12243) were utilized to purify plasmid DNAs following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Kit (Qiagen, Germany) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Transcription kit (Qiagen) and prepped for RT-PCR using PIPETMAX (Gilson ...
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bioRxiv - Bioengineering 2022Quote: ... Transcription Kit (QIAGEN). qPCR was performed on the StepOne™ system (Applied Biosystems ...
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bioRxiv - Genomics 2019Quote: ... Transcription kit (Qiagen). PCR reactions were done with the DyNAzyme II DNA Polymerase (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli DH5α cells and isolated by Qiagen miniprep kit (Qiagen), quantified using a nanodrop spectrophotometer ...
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bioRxiv - Molecular Biology 2021Quote: ... RNeasy kit (Qiagen) was used purify the RNA for whole cell steady state mRNA sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... Transcription Kit (QIAGEN), quantitative PCR was performed on the StepOne™ system (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Tissue kit (Qiagen) following instructions from the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... Transcription Kit (Qiagen), followed by qPCR with QuantiNova SYBR Green PCR Kit (Qiagen) ...