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Citations for Qiagen :
1 - 50 of 10000+ citations for Alkaline Phosphatase Colorimetric Activity Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and the GFP expression vector pEGFP-N1 were linearized with DraIII and treated with alkaline phosphatase before purifying with a PCR purification kit (Qiagen). Then 500 ng each of circular or linearized pGEM-Dik-LT/O and pEGFP-N1 were co-transfected into 293 cells with Lipofectamine 2000 reagent according to the manufacturer’s instruction and the medium was replaced with fresh DMEM four hours after transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... we midi-prepped these cultures via alkaline lysis (Qiagen Plasmid Plus Midi-Prep Kit). This first cloning step enabled generation of intermediate products ...
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bioRxiv - Microbiology 2021Quote: ... lysed using alkaline lysis in buffer P2 and neutralized with buffer N3 (All reagents from QIAprep kits, QIAGEN). Following addition of buffer N3 ...
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bioRxiv - Microbiology 2020Quote: ... or 7-recrudescent populations as well as PruQ tachyzoites and alkaline-induced PruQ bradyzoites (pH8.2 media for 72 h) were purified using the RNeasay kit (Qiagen) according to manufacturer protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... Isolated colonies were inoculated into 5 mL of LB with appropriate antibiotics and plasmids were purified using the alkaline lysis protocol and Qiaquick miniprep kit (Qiagen). Plasmids were verified by sequencing (GATC ...
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bioRxiv - Genetics 2019Quote: ... IPA Pathways Activity Analysis module (QIAGEN) was used to identify canonical pathways effected by the genes involved in baseline refractive eye development or regulating susceptibility to myopia ...
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bioRxiv - Bioengineering 2019Quote: ... The bacterial pellet was processed to purify all containing DNA by alkaline lysis and column purification (Qiagen miniprep spin kit, Qiagen, Inc., Germany).
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bioRxiv - Neuroscience 2020Quote: ... The IPA Pathways Activity Analysis module (QIAGEN) was used to identify canonical pathways encoded by the genes involved in baseline refractive eye development ...
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bioRxiv - Microbiology 2019Quote: ... protease and phosphatase inhibitors) or RLT buffer supplemented with “- mercaptoethanol (RNeasy mini kit, Qiagen, Hilden, Germany) for western blot analysis and total RNA extraction ...
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bioRxiv - Cancer Biology 2019Quote: Transcription activity of EGR1 was measured using the dual luciferase assay with the Cignal EGR1 Reporter Assay Kit (Qiagen). MIR2052HG knocked-down MCF7/AC1 and CAMA-1 cells were transfected with either EGR1 reporter (EGR1-responsive GFP reporter) ...
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bioRxiv - Biochemistry 2020Quote: ... The relative TCF/LEF reporter activity was measured by the Cignal™ TCF/LEF Reporter Assay Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Bacmid DNA was isolated via alkaline lysis (Qiagen buffers P1, P2 and P3) and isopropanol precipitation ...
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bioRxiv - Immunology 2023Quote: ... BAC DNA was purified from 200ml bacterial cultures by alkaline lysis (Qiagen buffers), and circular DNA was separated by CsCl ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... The effect of IscR-C92A on σ70-dependent activity was determined by incubating IscR-C92A with 2 nM supercoiled pPK12778 [purified with the QIAfilter Maxi kit (Qiagen)] ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 U HotStar Taq (Qiagen; based on polymerization activity), and 32 U FEN1 (New England Biolabs) ...
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bioRxiv - Genomics 2019Quote: ... the cells were incubated in an alkaline solution (buffer DLB and DTT, Qiagen, Germany) for 5 min at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... The IPA Pathways Activity Analysis module (Ingenuity Pathway Analysis, QIAGEN) was used to identify canonical pathways encoded by the genes involved in baseline refractive eye development ...
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bioRxiv - Neuroscience 2022Quote: ... The transcribed RNA was treated with Turbo DNase I and antarctic phosphatase to remove template DNA and purified by RNeasy MinElute Cleanup Kit (QIAGEN). The concentration was determined by Quibit microRNA Assay Kit ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were subject to alkaline lysis by adding 250ul of P2 lysis buffer (Qiagen, 19052) for 5 mins and then neutralized by addition of Qiagen N3 neutralization buffer (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... RNA was removed by alkaline hydrolysis and cDNA was purified with PCR cleanup columns (Qiagen 28115). Next ...
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bioRxiv - Bioengineering 2019Quote: ... The bacterial pellet was processed to purify all containing DNA by alkaline lysis and column purification (Qiagen miniprep spin kit ...
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bioRxiv - Microbiology 2021Quote: ... RNA was removed by alkaline hydrolysis and cDNA was purified with PCR clean-up columns (Qiagen; #28115). Next ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were amplified in Escherichia coli (XL-Blue) extracted by alkaline lysis and purified by column separation (Qiagen). The integrity was confirmed by gel electrophoresis in 0.8% agarose ...
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bioRxiv - Microbiology 2022Quote: ... Nuclease activity was immediately inactivated by adding guanidium-thiocyanate containing lysis buffer (Qiagen), followed by total nucleic acid extraction of 400 ul of pretreated stool using the EZ1 Virus Mini Kit v2.0 (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... phosphatase inhibitors 2 and 3) and then passing through Qiashredder columns (79656, Qiagen). Tissue samples were weighted ...
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bioRxiv - Neuroscience 2023Quote: ... Pathway (biological functions and diseases) enrichment and activity analyses of overlapping genes were performed using Ingenuity Pathway Analysis (Qiagen Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... Gene set enrichment analysis overlap and activity z-scores p values have been calculated using the Ingenuity Pathway Analysis Software (Qiagen) using the previously described method (76) ...
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bioRxiv - Cell Biology 2020Quote: ... the CREB pathway reporter and the NF-κB pathway reporter that measure the activity of the corresponding pathway were purchased from Qiagen. The primers used for vector construction are listed in Supplementary table 3.
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bioRxiv - Cancer Biology 2022Quote: ... Tissue was homogenized in 150uL of RIPA buffer with protease and phosphatase inhibitors by bead homogenizer (Tissue LyserII, Qiagen) for 3 mins at a frequency of 30 hz/s and immediately cooled for 5 mins on ice ...
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bioRxiv - Pathology 2020Quote: 200 microgram of kidney tissue were homogenized and lysed by 8M urea in 20mM HEPES (pH=8.0) buffer with protease and phosphatase inhibitors using Tissue Lyser II (QIAGEN). Samples were reduced and alkylated followed by MS-grade trypsin digestion ...
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bioRxiv - Physiology 2021Quote: Lyophilized mouse hearts were homogenized in RIPA buffer supplemented with protease and phosphatase inhibitors (Pierce) using a TissueLyser II apparatus (Qiagen), followed by sonication and centrifugation (10 min at 12,000 rpm at 4°C) ...
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bioRxiv - Cell Biology 2021Quote: ... Muscle samples were next lysed in ice-cold radioimmunoprecipitation assay (RIPA) buffer (400 μL/muscle) with protease and phosphatase inhibitor cocktail (Cell Signalling) using TissueLyser II (QIAGEN). Half of the lysate was mixed with scintillation cocktail for scintillation counting using Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Cell Biology 2022Quote: ... protease and phosphatase inhibitors (PMSF, Aprotinin, Pepstatin, Leupeptin) and homogenised with autoclaved metal beads in a TissueLyser II instrument (Qiagen, Hilden, Germany), followed by ultrasonication (8 sec ...
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bioRxiv - Genomics 2019Quote: The RNA extraction kit (RNeasy Mini Kit, Qiagen) was used for the total RNA isolation ...
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bioRxiv - Bioengineering 2020Quote: ... An RNA extraction kit (RNeasy extraction Kit, Qiagen) was used to purify RNA ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription kits (RT2 First Strand Kit; Qiagen) were used for cDNA synthesis ...
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bioRxiv - Microbiology 2019Quote: ... kit (Qiagen). Phylogenetic groups were determined as described in (Clermont ...
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bioRxiv - Microbiology 2019Quote: ... Kit (QIAGEN) followed with 16S-ITS PCR as previously described (64 ...
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bioRxiv - Genetics 2020Quote: ... Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Kit (Qiagen). Whole-genome sequencing was performed using the Illumina Nextera XT library protocols and sequenced at 2 x 300bp read length on the MiSeq platform (Ramaciotti Centre for Functional Genomics ...
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bioRxiv - Microbiology 2024Quote: ... Kit (Qiagen) using 5 mL culture of V_227 which was grown in MMT-YE medium (20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The kits for RNA extraction were from Qiagen (RNeasy Mini Kit and RNeasy Micro Kit). The reverse transcription kit ...
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bioRxiv - Neuroscience 2021Quote: ... Using a commercial kit (QIAGEN DNeasy isolation kit #69506), DNA was isolated from a tissue punch taken from the external ear ...
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bioRxiv - Microbiology 2023Quote: ... using a commercial kit (RT2 First Strand Kit, Qiagen). Quantification of transduction of EBECs and HBECs with the SARS-CoV-2 pseudovirus was determined by real-time qPCR (rt-PCR ...
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bioRxiv - Microbiology 2023Quote: ... and the RNeasy kit plus Mini kit (#74134, Qiagen) or using NucleoSpin RNA Plus XS ...
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bioRxiv - Genetics 2019Quote: ... DNA maxiprep kit was from Qiagen (EndoFree Plasmid Maxi Kit). All plasmids were cloned with Gibson assembly (42 ...
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bioRxiv - Cell Biology 2021Quote: ... QIAquick PCR purification kit or QIAquick Gel Extraction kit (Qiagen). Cloning strategies for each construct are described in Table S1 ...
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bioRxiv - Genetics 2022Quote: ... we used the QIAGEN kit (DNeasy Blood & Tissue Kit; Qiagen, https://www.qiagen.com/ ...