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Citations for Qiagen :
1 - 50 of 5264 citations for African Swine Fever Virus P30 Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: RNA from Cebpap30/p30 cell lines was isolated using RNeasy Plus Mini Kit (#74134 Qiagen) according to the manufacturer’s instructions and converted to cDNA with RevertAid First Strand cDNA Synthesis Kit (K1622) ...
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bioRxiv - Cell Biology 2020Quote: ... Spike-in Cel-miR-39 was added to cell conditioned media (CCM) and explanted swine aortic valves for normalization and quality control (219610, QIAGEN, Germany). All reactions were performed in technical triplicates ...
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bioRxiv - Genomics 2019Quote: ... Insects were immediately dissected in RNAlater (Qiagen) to stabilize expression profiles and kept deeply frozen until RNA extractions ...
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bioRxiv - Microbiology 2020Quote: ... Virus RNA virus was extracted by Qiamp viral RNA (Qiagen) and quantified using Qbit 3 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... virus media was removed and cells were harvested using RNAprotect Cell Reagent (Qiagen). RNA was extracted using RNeasy columns (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Insect cells were transfected with SMS/pMT/V5-HisB constructs using Effectene (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: Proteins were purified from Sf21 insect cells by affinity purification with Ni-NTA agarose (Qiagen, 30230) and ...
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bioRxiv - Immunology 2020Quote: ... HIS-tagged DR protein monomers were produced by Hi5 insect cells and purified using Ni-NTA (Qiagen) and size exclusion columns (AKTA ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was supplemented with Ni-NTA agarose (Qiagen, 1.3 ml resin/ 1 l insect cell pellet). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... swine DNA was extracted from nasal turbinates and lungs using DNeasy Blood & Tissue kit (Qiagen, Germantown, MD, USA) and subjected to PCR with primers (Forward primer ...
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bioRxiv - Microbiology 2020Quote: ... virus medium was aspirated and cells were harvested using 400 µL of RNAprotect Cell Reagent (Qiagen). RNA was extracted using the Qiagen RNAeasy Mini kit ...
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bioRxiv - Microbiology 2023Quote: ... total RNA from virus-infected cells was extracted using the RNeasy kit (Qiagen) or Isolate II RNA mini kit (Bioline) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet, Qiagen) and incubated for 30 mins at 4 °C on a rotating wheel ...
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bioRxiv - Immunology 2024Quote: ... T157I mutations (23) were produced by Hi5 insect cells and extracted from culture supernatant using Ni-NTA (Qiagen). HLA-DR monomers were biotinylated overnight at 4°C using BirA biotin ligase (Avidity ...
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bioRxiv - Microbiology 2020Quote: ... Whole insects were homogenised using a TissueLyser® (Qiagen, UK) with one or two steel beads of 3 mm (Dejay Distribution ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was extracted from virus-infected cells using an RNeasy Mini Kit (Qiagen). For PB2 ...
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bioRxiv - Microbiology 2020Quote: ... nasal swab samples or cell cultured virus isolates by RNeasy Mini Kit (QIAGEN, Denmark) automated on the QIAcube (QIAGEN ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The insect material was homogenised using a TissueLyser (Qiagen, Haan, Germany) with a metallic bead ...
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bioRxiv - Evolutionary Biology 2019Quote: ... QIAsymphony DSP virus/pathogen Kit (Qiagen) for blood samples ...
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bioRxiv - Microbiology 2023Quote: ... with EZ1 DSP Virus kit (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... using Virus Extraction Mini Kit (Qiagen). The RT-qPCR assay were performed with a SuperScript III Platinum One-Step RT-qPCR Kit with ROX (Invitrogen - Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from frozen retina of 30 day-old mice (P30) mice with RNeasy lipid tissue mini kit (Qiagen). cDNA was generated with the Quantitect Reverse transcription kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from virus supernatants using QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). qPCR was then performed using AgPath RT-PCR (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted from SIV and SIVΔNef virus stocks with the QIAamp UltraSens Virus kit (Qiagen, Germantown, MD), reverse transcribed with the Superscript VILO kit (Thermofisher ...
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bioRxiv - Microbiology 2019Quote: ... with the Virus Mini Kit v2.0 (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... ZIKA Viral RNA was isolated from Zika virus stock using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) to create a standard curve using serial 10-fold dilutions of ZIKV RNA ...
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bioRxiv - Microbiology 2022Quote: ... copies were extracted from plasma virus using the Qiagen BioRobot EZ1 Workstation with EZ1 Virus Mini Kit v2.0 (Qiagen). Eluted vRNA was subsequently used as a template for cDNA synthesis and reverse-transcribed using the reverse primer SHIV.Env.R1 (5’-TACCCCTACCAAGTCATCA-3’ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Insect DNA was extracted using a DNeasy Blood and Tissue Kit (Qiagen, Germany). After extraction ...
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bioRxiv - Genetics 2024Quote: ... Insects were homogenized for 3 min at 30 Hz with TissueLyser II (Qiagen), using three 3 mm steel beads (TIS GmbH ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA was extracted from 10 ul of the purified virus using the MinElute Virus Spin Kit (Qiagen Cat#57704), and the viral genome titer was determined by qPCR using an AAV2 rep gene specific primer probe set (repF ...
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bioRxiv - Genetics 2020Quote: ... Total viral RNA was extracted from patient specimens and tested for the presence of SARS-CoV-2 with an EZ1 virus extraction kit and EZ1 Advanced XL instrument with the Virus card (Qiagen), or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... For next generation sequencing RNA from virus-containing samples were extracted using the QIAsymphony DSP Virus/Pathogen mini kit (Qiagen). RNA was DNase-treated using the TURBO-free Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Whole genome sequencing of the isolated virus was done isolating viral RNA using the QIAamp MinElute Virus Spine kit (Qiagen) and performing Illumina sequencing ...
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bioRxiv - Genetics 2022Quote: ... The QIAamp MinElute Virus Spin Kit (QIAGEN, Germany) was used for viral nucleic acid extraction ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from mock and virus infected Vero cells using the RNeasy mini kit (Qiagen, Germany). Next ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from cell-free virus-containing supernatants using the viral RNA extraction mini kit (Qiagen). cDNA synthesis was conducted using Superscript IV and the uni12-FluG primer (AGCGAAAGCAGG) ...
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bioRxiv - Microbiology 2022Quote: Total virus-RNAs were extracted from SK.N.SH infected cells at MOI 0.5 using RNeasy minikit (Qiagen, #74104). ISRE reporter assay was performed in STING37 cells plated in 24 wells plate (2×105 cells/well ...
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bioRxiv - Molecular Biology 2023Quote: ... pooled insects whole bodies or tissues were homogenized using the Tissue Lyser II (Qiagen) in a 1.7 ml microcentrifuge tube containing 500 µl of ice-cold trizol and five three-millimeter glass beads (Pyrex) ...
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bioRxiv - Microbiology 2022Quote: Viral RNA from cell culture supernatants was isolated as the manufacturer’s instructions using QIAamp MinElute Virus kit (Qiagen). Cells were suspended in TRI Reagent (Sigma Aldrich) ...
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bioRxiv - Genetics 2020Quote: ... or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... QIAamp MinElute virus spin kit (Qiagen Sciences, Maryland, MD) was used to extract total nucleic acids from each wastewater sample as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and a dedicated kit (QIAsymphony DSP Virus/Pathogen, Qiagen). All the extracts were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... Equine arteritis virus (EAV) in AVL lysis buffer (Qiagen) was spiked into the reagent as internal control for extracellular RNA samples ...
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bioRxiv - Microbiology 2021Quote: ... Virus RNA was extracted by Qiamp viral RNA (Qiagen) and quantified using Qbit 3 Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... using the “QIAamp Min ELUTE Virus Spin” Kit (QIAGEN), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... using the QIAamp 96 Virus kit QIAcube HT (Qiagen). To detect SARS-CoV-2 RNA ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was extracted from the challenge virus inoculum and virus-positive clinical samples using the QIAamp viral RNA mini kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... using the EZ1 Virus Mini Kit v2.0 (Qiagen Hilden, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... and QiaAmp 96 Virus QiaCube HT kit (Qiagen, Cat. 57731). RT-PCR reaction was setup using SensiFast Probe No-ROX One- Step Kit (Bioline ...
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bioRxiv - Genomics 2021Quote: ... the insect tissue was homogenized and the genomic DNA was isolated using Qiagen Genomic-tip (Qiagen). Later ...