Labshake search
Citations for Qiagen :
1 - 50 of 376 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... we extracted total RNA from CD4+/CD8+/CD3+ T cells (from spleen or bone marrow) of Foxp3-GFP or C57BL/6 mice using RNeasy Micro Kit (Qiagen) and cleaned from excess DNA with DNAse 1 enzyme (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from CTi400 and N/TERT-1 cells using RNeasy Plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: RNA from cultured GCs was isolated using RNeasy Micro Kit (Qiagen #74004) with on-column DNA digestion ...
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bioRxiv - Cell Biology 2021Quote: Isolated Tert+ and Tert-cells (≤ 5 cells) were subjected to synthesize the complementary DNA (cDNA) using REPLI-g WTA Single Cell Kit (QIAGEN) and analyzed for gene expression by qRT-PCR ...
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bioRxiv - Neuroscience 2022Quote: ... GCs were sorted directly into the RLT lysis buffer (QIAGEN Allprep Mini Kit) for total RNA extraction.
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bioRxiv - Microbiology 2021Quote: ... 6 (Qiagen) for additional analysis ...
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bioRxiv - Neuroscience 2023Quote: ... fluorescently labeled GC particles were collected in RLT (Allprep DNA/RNA Micro Kit #80284, Qiagen) supplemented with 10% b-Mercaptoethanol.
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from cultured in vitro or in vivo primed T cells or ex vivo sorted T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: DAFhi and DAFlo GC B cells (CD19+ CD20+ CD38+ IgD−) were resuspended in RLT cell lysis buffer (Qiagen) after flow cytometric sorting ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from 18 ONS cell samples (6 AD patients, 6 MCI and 6 HC) were extracted using Allprep universal kit (Qiagen cat. no. 80224). RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA library Prep Gold Kit (Illumina 20020598 ...
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bioRxiv - Systems Biology 2020Quote: ... 6×10^6 PBMC from each sample were transferred directly to Quiazol regent (QIAGEN), resuspended and immediately aliquoted and stored at −80°C until RNAseq downstream processing ...
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bioRxiv - Molecular Biology 2020Quote: 6 ml Ni-NTA (QIAGEN) was washed with 5 column volumes of wash buffer 1 (150 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Gene ontology analyses for high affinity/SHM class-specific GC B cells were performed with Ingenuity Pathway Analysis (Qiagen) software using avg_logFC values of all genes significantly enriched in at least one class ...
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bioRxiv - Cancer Biology 2019Quote: ... Pathways significantly affected in non-T cell-inflamed or non-T cell-inflamed-enriched mutated genes were identified by IPA® (QIAGEN Inc., Germany).
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bioRxiv - Immunology 2021Quote: RNA was prepared from sorted GC B cells and LNPCs from FNA or enriched BMPCs from bone marrow using the RNeasy Plus Micro kit (Qiagen). Libraries were prepared using the NEBNext Immune Sequencing Kit for Human (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... PMSG- or hCG-regulated genes in wildtype GCs that showed a differential expression in Erβnull GCs were selected as ERβ-regulated genes and subjected to Ingenuity Pathway Analysis (IPA; Qiagen Bioinformatics) to determine their involvement in folliculogenesis.
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bioRxiv - Immunology 2021Quote: Total RNA from sorted GC B-cells of immunized WT and CD22KO mice was extracted using an RNeasy micro kit (Qiagen). Indexed cDNA libraries were generated using a SMART-Seq Stranded kit (Takara Bio ...
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bioRxiv - Cancer Biology 2019Quote: ... T cells were sorted directly into 250μl RNAprotect buffer (Qiagen), spun down for 1 minute at 2000 RPM ...
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bioRxiv - Plant Biology 2020Quote: Total RNA from Arabidopsis seedlings (Col-0, sphk1-2, SPHK1-KD and gcs-2) were extracted using RNeasy® Plant Mini Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Purified CD4+ T cells were homogenized using the Qiashredder (Qiagen, 79654) and RNA was extracted using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... We performed PCR reactions using the Taq Polymerase Phusion® High-Fidelity PCR Master Mix with GC buffer and prepared them according to the manufacturer’s instructions (Qiagen, Hilder, Germany). PCR amplification steps involved an initial denaturing step for 30 sec at 98°C ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of RGS(His)6-tagged 14-3-3 was performed by applying the anti-RGS(His)6 antibody (Qiagen) in combination with a secondary anti-mouse HRP antibody.
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bioRxiv - Genetics 2020Quote: ... coli strains expressing (His)6-Clr4 and (His)6-Swi6 and allowed to bind at 4°C overnight to Ni-NTA resin (Qiagen; binding capacity 5-10mg protein/ml resin) ...
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bioRxiv - Microbiology 2023Quote: ... The SCGs cultured in 6-well dishes and one entire 6-well was harvested and pooled together for RNA isolation (RNeasy, Qiagen) per sample ...
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bioRxiv - Immunology 2020Quote: ... Sorted T cells were lysed in Buffer RLT Plus (Qiagen, Hilden, Germany) with 1% β-mercaptoethanol (Millipore-Sigma ...
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bioRxiv - Physiology 2020Quote: ... and Student’s t tests for significance per the manufacturer’s analytical software (Qiagen), with results plotted as fold change showing 95% confidence limits ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1 μM Locked Nucleic Acid (LNA) oligo-d(T)30 (Qiagen) in primary-probe hybridization buffer composed of 40% formamide (Sigma ...
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bioRxiv - Immunology 2020Quote: ... or CD4+ T cells was extracted using the RNeasy micro kit (Qiagen) followed by cDNA synthesis using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Immunology 2021Quote: ... and an undisclosed peptide pool inducing CD8+ T lymphocyte stimulation (Qiagen, 2017); rmsHBHA which tubes contain recombinant M ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from isolated CD8+ T cells (RNeasy kit, Qiagen) and 300 ng of RNA were used for cDNA synthesis (First-Strand Synthesis kit ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from murine T cells via RNeasy Kit (Qiagen) with DNase digestion (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... and CD95+ CD4 T cells using a RNeasy plus mini kit (QIAgen). Isolated RNA sample quality was assessed using a BioAnalyzer RNA pico assay (Agilent Technologies Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... 6 µl Hi-Perfect transfection reagent (Qiagen, 301707), and 1 µl of the desired (20 µM ...
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bioRxiv - Immunology 2024Quote: ... total mRNA was isolated from mouse T cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Immunology 2024Quote: ... total RNA was extracted from T cells by using RNeasy mini kit (QIAGEN) and reverse-transcribed into cDNA with Superiorscript III reverse transcriptase (Enzynomics ...
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bioRxiv - Immunology 2024Quote: T cells were subjected to RNA extraction utilizing the RNeasy Micro Kit (QIAGEN). RNA from the renal cortex was isolated with the NucleoSpin Kit (Macharey-Nagel ...
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bioRxiv - Immunology 2021Quote: ... total RNA of CD8+ human T cells was extracted by miRNeasy Mini Kit (Qiagen), following the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: Cultured human T cells after treatment were flash frozen in Buffer RLT (Qiagen, 79216) and kept in −80°C until processing ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from human primary T cells was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from sorted CD4+ T cells using the RNeasy Mini kit (Qiagen). A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen) ...
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bioRxiv - Genomics 2022Quote: ... 400 nL of protease mix (6 μg protease (Qiagen, 19155), 6.25x NEBuffer 4 (NEB ...
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bioRxiv - Microbiology 2021Quote: ... 6 and 9 employing the RNeasy plant mini kit (Qiagen). RNA was quantified using Nanodrop (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... For MUS81 interference it was used FlexiTube HsMUS81 6 (Qiagen). SMARCAL1 was depleted using the MISSION esiRNA HUMAN SMARCAL1 (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... 6 million PBMCs were lysed using QIAzol Lysis Reagent (Qiagen). Samples were stored at -80°C until RNA extraction ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µg C19orf43 (purified from E. coli BL21 through Qiagen Ni-TA agarose using standard procedures ...
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bioRxiv - Biochemistry 2023Quote: ... 6 mL of Ni2+-NTA slurry (Qiagen, Venlo, LI, Netherlands) were equilibrated in a gravity flow column with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... UniSpike 6 (included in the QIAGEN miRCURY LNA RT Kit) to verify the efficacy of reverse transcription (RT ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from CD4+ T cells using the DNeasy blood and tissue kit (Qiagen), according to manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... The total RNA of sorted T cells was extracted with an RNeasy Micro kit (Qiagen) and reverse transcribed using reverse transcribed using SuperScript III Reverse Transcriptase (Invitrogen) ...