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Citations for Qiagen :
1 - 50 of 1270 citations for 6 Isopropyl 2 methoxy phenol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... phenol-chloroform extraction and resuspended in EB Buffer (Qiagen, Buffer EB - Elution Buffer ...
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bioRxiv - Cancer Biology 2023Quote: 2 × 105 cells were reverse-transfected in 6-well plates with Flexitube siRNA constructs (Qiagen) targeting KEAP1 (KEAP1_5 or KEAP1_8) ...
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bioRxiv - Immunology 2019Quote: ... and phenol chloroform extraction followed by an RNAeasy Kit (Qiagen). cDNA was synthesized using a High Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2019Quote: RNA was extracted from lung samples using phenol (QIAzol, 79306, Qiagen) and choloform extraction and a TissueLyzer (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA was further purified using phenol chloroform/PCR Purification Kit (QIAGEN) and analyzed by PCR on a QuantStudio 7 Flex Real Time PCR System (Applied Biosystem) ...
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bioRxiv - Microbiology 2021Quote: ... 6 (Qiagen) for additional analysis ...
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bioRxiv - Systems Biology 2020Quote: ... DNA was then purified by phenol-chloroform extraction using Maxtract tubes (Qiagen), and ethanol precipitated overnight ...
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bioRxiv - Microbiology 2023Quote: ... frozen liquid samples using the phenol-free RNeasy Plus Mini Kit (Qiagen) according to the instructions of the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... RNAs were isolated from 6-month H19ΔICR/H19ΔICR and H19+/H19+ littermates (2 per genotype) using RNeasy Plus Mini Kit (Qiagen). Samples with RNA Integrity numbers >9 were Ribosomal RNA depleted using RiboZero Gold Kit (Illumina) ...
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bioRxiv - Neuroscience 2021Quote: ... We pooled 2-3 fishes (6 weeks old) and extracted whole RNA using the RNAEasy Micro kit (Qiagen), with blood ...
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bioRxiv - Microbiology 2021Quote: ... The pooled RNA was precipitated by isopropyl alcohol with addition of glycogen followed by additional clarification with RNeasy MinElute Cleanup kit (Qiagen, Germany). NEBNext rRNA Depletion kit (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... Phenol chloroform extraction was then used together with the RNeasy mini kit (QIAGEN) to isolate and purify RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was purified by phenol chloroform extraction and QiaQuick PCR Purification Kit (Qiagen).
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bioRxiv - Genomics 2019Quote: ... DNA was purified by phenol-chloroform-isoamyl extraction followed by MinElute column (Qiagen) clean up ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was then purified by phenol-chloroform extraction using Maxtract Tubes (129046, Qiagen) and pellet resuspended in 36.5 μl TE Buffer.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... we used a phenol-chloroform extraction with Qiagen RNAeasy kits (Qiagen, Hilden, Germany) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated at 6 time points (0, 0.5, 1, 2, 4, and 8 hours) using the RNeasy Mini kit (Qiagen) according to the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) into the LB media and purified by Ni-NTA affinity chromatography (Qiagen, USA). The full length of ASSCP2 protein (ASSCP2-R ...
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bioRxiv - Biochemistry 2021Quote: ... and RNA was purified by phenol-chloroform extraction using MaXtract high density tubes (Qiagen). RNA was treated with Turbo DNase (Thermo Fisher ...
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bioRxiv - Genomics 2022Quote: ... The 3C libraries were purified by phenol-chloroform extraction and PCR purification kits (QIAGEN). The 3C interactions were then detected by qPCR with the use of a Kapa SYBR Fast qPCR kit.
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bioRxiv - Developmental Biology 2020Quote: ... followed by phenol-chloroform extraction and processing with the RNeasy Mini Kit (74104, Qiagen). RNA quality and quantity was determined using a NanoDrop spectrophotometer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: RNA was extracted with the phenol-chloroform method according to the manufacturer’s protocol (Qiagen) with small modifications ...
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bioRxiv - Neuroscience 2023Quote: ... and we immediately mechanically dissociated the cells in phenol and guanidine thiocyanate (QIAzol, QIAGEN) which facilitate lysis of fatty tissues and inhibit RNases ...
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bioRxiv - Genetics 2024Quote: ... and purified by phenol/chloroform extraction using an RNeasy Mini Kit (QIAGEN, Venlo, Netherlands).
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bioRxiv - Molecular Biology 2024Quote: ... MtDNA was isolated by phenol/chloroform extractions or by Puregene Core Kit A (QIAGEN) and radiolabeled replicated DNA was analyzed by Southern/ D-loop Southern blotting (described earlier ...
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bioRxiv - Cancer Biology 2024Quote: ... Phenol-chloroform/chloroform extraction was then performed using MaXtract High Density tubes (Qiagen, 129073). Tubes were pre-spun according to the manual ...
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bioRxiv - Neuroscience 2021Quote: Fluorophore TYE665 labeled locked nuclear acid (LNA) (C4G2)6 and (CCG)8 probes (Supplemental table 2) were synthesized by Qiagen. The FISH protocol was adapted from (DeJesus-Hernandez et al ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells in 6-well plates were transfected with 2 μg pIRES2-eGFP or Trim-HA-NUP153 derivatives using Effectene (Qiagen). At 24 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: 3×106 cells from the day 8 of CD34+ HSPC and day 6 of HUDEP-2 erythroid differentiation were used for total RNA using RNeasy Mini Kit (Qiagen). For reverse transcription using Primescript RT reagent kit (Takara Bio Inc.) ...
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bioRxiv - Cell Biology 2019Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates or in wells of a 6-well plate using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from cells (1-2 wells, 6 well plate) using an RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), including a DNase step to remove residual DNA ...
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bioRxiv - Immunology 2023Quote: ... in vitro derived Foxp3+ cells were harvested on day 2 and day 6 into Trizol and total RNA was isolated with miRNeasy Micro Kits (QIAGEN 217084) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... phenol and column purification protocol using the QIAquick 96 PCR purification kit (Qiagen, Hilden, Germany), as described in Text S1 of Kittelmann et al ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted in 150 μl phenol-chloroform and purified on a QIAquick column (Qiagen) and eluted in 50 μl EB buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated using the phenol/guanidine based QIAzol lysis reagent (Qiagen, Valencia, CA), according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from 18 ONS cell samples (6 AD patients, 6 MCI and 6 HC) were extracted using Allprep universal kit (Qiagen cat. no. 80224). RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA library Prep Gold Kit (Illumina 20020598 ...
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bioRxiv - Systems Biology 2020Quote: ... 6×10^6 PBMC from each sample were transferred directly to Quiazol regent (QIAGEN), resuspended and immediately aliquoted and stored at −80°C until RNAseq downstream processing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... One million cells per well were seeded in 6 well plates in 2 mL growth medium the day before transfection and transfected with PolyFect (Qiagen, Düsseldorf, DE) using the manufacturers protocol (changing the medium to growth medium without hygromycin B and puromycin prior to transfection) ...
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bioRxiv - Molecular Biology 2020Quote: 6 ml Ni-NTA (QIAGEN) was washed with 5 column volumes of wash buffer 1 (150 mM NaCl ...
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bioRxiv - Systems Biology 2020Quote: ... DNA was recovered by phenol extraction followed by a Qiaquick purification (PCR purification columns, Qiagen, Germany). Specific sequences in the immunoprecipitates were quantified by real-time PCR using the primers listed below ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from 2OD of cells using acid phenol and purified using RNeasy kit (Qiagen). Reverse transcription was performed on 750ng of RNA using SuperScript III First-Strand Synthesis SuperMix (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... and column purified to remove DNA and trace phenol using a RNeasy Mini Kit (Qiagen, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted using the standard phenol-chloroform procedures (Trizol) and the RNeasy kit (Qiagen). For the RT² Profiler™ PCR Array Human Epithelial to Mesenchymal Transition kit (EMT ...
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bioRxiv - Immunology 2023Quote: ... DNA was isolated from stool samples using phenol/chloroform and the QIAquick PCR purification kit (Qiagen). For 16S rDNA profiling ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA extraction was performed using phenol method and retro-transcribed with QuantiTect Reverse Transcription Kit (Qiagen). Standard curves of WT genomic DNA (20/0,05 ng ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated via chloroform-phenol phase separation followed by processing via the RNeasy isolation kit (Qiagen). Contaminating genomic DNA was digested using the Turbo DNA-free kit (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... rRNAs from pre-40S intermediates were phenol chloroform extracted and further cleaned using RNeasy Mini columns (Qiagen). 1ug of rRNA was used for each reverse transcription reaction using a primer that starts at 1241 of 18S rRNA (sequence listed in Table S4) ...
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bioRxiv - Microbiology 2021Quote: RNA was purified by acid phenol-chloroform extraction and column purified (RNeasy mini kit QIAGEN REF 74104) from HFFs grown in media with or without Pan and/or infected with wild-type RH parasites (grown in regular or Pan-depleted media) ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted using the phenol-chloroform protocol combined with Phase Lock Gel tubes (Qiagen GmbH). Libraries were prepared using a 1D ligation sequencing kit (SQK-LSK-108 ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was prepared via a modified partial phenol-methanol extraction protocol (RNeasy Micro Kit, Qiagen, USA). Purity and quality of RNA were assessed by determining the optical density (OD ...