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Citations for Qiagen :
1 - 50 of 377 citations for 6 β NALOXOL HCL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The expressed recombinant protein was solubilized in a denaturing buffer (6 M HCl-Guanidine, 20 mM Tris-HCl[pH 7.5] and purified by Ni-NTA (30210, QIAGEN) under denaturing conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... solubilized in a denaturing buffer (6 M HCl-Guanidine, 20 mM Tris-HCl pH 7.5) and purified by Ni-NTA (QIAGEN) under denaturing conditions ...
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bioRxiv - Microbiology 2021Quote: ... IL-6 and β-actin cDNA were determined by QuantiFast SYBR Green PCR Master Mix (Qiagen, Hilden, Germany) at the conditions ...
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bioRxiv - Cell Biology 2023Quote: ... RNA and protein were digested in 40 mM Tris-HCl pH 6 and 10 mM EDTA by adding first RNAse A (Qiagen; #19101, 1.5 hours, 37°C), followed by Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... The eluates were mixed with guanidine– HCl to a final concentration of 6 M and incubated with Ni-NTA sepharose (Qiagen, 100 μl of slurry per sample) o/n at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 M Tris/HCl pH 8.5 (JCSG+ suite, Qiagen). Hanging drops of 1 ml protein at 2 mg/ml and 1 ml of precipitant (19% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... 6 (Qiagen) for additional analysis ...
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bioRxiv - Microbiology 2020Quote: ... mouse β-actin (PPM02945B-200, Qiagen), or MHV-A59 N gene using RT2 SYBR Green qPCR Mastermix (330502 ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Buffer RLT + β-mercaptoethanol (Qiagen), snap frozen on dry ice ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer RLT + β-mercaptoethanol (Qiagen), and snap-frozen on dry ice ...
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bioRxiv - Immunology 2020Quote: ... β-actin (pre-designed primers from Qiagen); HPRT(Eun et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... or RLT-Buffer with β-mercaptoethanol (Qiagen). Parasite RNA was isolated with the RNeasy Kit (Qiagen ...
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bioRxiv - Bioengineering 2020Quote: ... 0.001 M of HCl at pH 3 was added and then homogenized (TissueRuptor, Qiagen) on ice ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended in 24μl EB buffer (Tris HCl pH=8.5; Qiagen Cat. No. 19086).
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bioRxiv - Neuroscience 2022Quote: Total RNA from 18 ONS cell samples (6 AD patients, 6 MCI and 6 HC) were extracted using Allprep universal kit (Qiagen cat. no. 80224). RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA library Prep Gold Kit (Illumina 20020598 ...
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bioRxiv - Systems Biology 2020Quote: ... 6×10^6 PBMC from each sample were transferred directly to Quiazol regent (QIAGEN), resuspended and immediately aliquoted and stored at −80°C until RNAseq downstream processing ...
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bioRxiv - Cancer Biology 2023Quote: ... Profiling of TGF-β targets was performed using the RT2 Profiler™ PCR Array Pig TGF-β Signaling Targets kit from Qiagen following manufacturers’ instructions.
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bioRxiv - Molecular Biology 2020Quote: 6 ml Ni-NTA (QIAGEN) was washed with 5 column volumes of wash buffer 1 (150 mM NaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... containing β-mercaptoethanol and the RNeasy Mini kit (Qiagen) followed by in-column DNase I treatment to remove genomic DNA contamination ...
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bioRxiv - Neuroscience 2023Quote: ... Buffer RLT with β-mercaptoethanol (Qiagen RNeasy Micro Kit) was added to the samples immediately after the last wash and tubes were vortexed to release the polysomes from the beads ...
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bioRxiv - Bioengineering 2020Quote: ... falciparum genomic DNA (gDNA) was diluted into elution buffer (10mM Tris-HCl, pH 8.5, QIAGEN) to stocks of 1E5 fg/μL ...
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bioRxiv - Genomics 2021Quote: ... These leftover materials were collected by LCM and placed into 350 μl of RLT lysis buffer (RNeasy, Qiagen, including 1%β-Mercaptoethanol (β-Me)) followed by RNA extraction and examination with the 2100 Bioanalyzer.
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bioRxiv - Immunology 2024Quote: ... TCRα/β were amplified using OneStep RT-PCR kit (Qiagen) according to the manufacturer recommendation ...
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bioRxiv - Microbiology 2021Quote: ... The eluate was supplemented with 30 mM Imidazole-HCl and incubated with Ni-NTA beads (Qiagen), 2 ml of beads for 500 ml culture ...
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bioRxiv - Systems Biology 2022Quote: ... 12 μl of 1 M Tris-HCl (pH 6.5) and 1 μl RNAse A (Qiagen cat # 19101) were added to the sample and incubated for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... then homogenized in RLT plus buffer with β (Qiagen, Hilden, Germany). Total RNA was then isolated from the RLT-homogenized cells using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Physiology 2021Quote: ... and 1% β-mercaptoethanol with a TissueLyser II (Qiagen, Manchester, UK) at maximum speed for 2 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Genomics 2021Quote: ... single cell directly into 2 µl lysis buffer (30 mM Tris-HCl [pH = 8.0], 10 mM NaCl, 0.2 µL Proteinase K [Qiagen, #19133] ...
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bioRxiv - Neuroscience 2020Quote: ... lumbar spinal cord or the glabrous skin of the footpad were harvested and homogenized in 0.1M HCl + 0.5% triton X-100 using glass beads and a TissueLyser II (Qiagen). Lysate was centrifuged at 18,000 xg and 4 °C for 40 – 60 min to pellet cell debris ...
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bioRxiv - Microbiology 2022Quote: ... The purified DNA pellets were resuspended in 60 µL Tris-HCl pH 7.5 with 0.8 mg/mL RNAse A (Qiagen) and incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Immunology 2021Quote: ... then homogenized in RLT plus buffer with β-mercaptoethanol (Qiagen, Hilden, Germany). Total RNA was then isolated from the RLT-homogenized cells using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 700 μL pre-cooled RLT + β-mercaptoethanol buffer (RNeasy Mini Kit, QIAGEN) was added and samples lysed with two 30 s Ribolyser pulses at speed setting 6.5 ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... containing 10μl of 1% v/v β-mercaptoethanol TCL buffer (Qiagen # 1031576) using a BD AriaII cell sorter ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of RGS(His)6-tagged 14-3-3 was performed by applying the anti-RGS(His)6 antibody (Qiagen) in combination with a secondary anti-mouse HRP antibody.
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bioRxiv - Genetics 2020Quote: ... coli strains expressing (His)6-Clr4 and (His)6-Swi6 and allowed to bind at 4°C overnight to Ni-NTA resin (Qiagen; binding capacity 5-10mg protein/ml resin) ...
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bioRxiv - Microbiology 2023Quote: ... The SCGs cultured in 6-well dishes and one entire 6-well was harvested and pooled together for RNA isolation (RNeasy, Qiagen) per sample ...
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bioRxiv - Neuroscience 2024Quote: ... 6 µl Hi-Perfect transfection reagent (Qiagen, 301707), and 1 µl of the desired (20 µM ...
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bioRxiv - Microbiology 2019Quote: ... Samples were neutralized with 0.7 µL of a 7.5 M HCl stock and the remaining cDNA was purified using a nucleotide removal kit (Qiagen). Sequencing reactions were performed according to the method of Sanger ...
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bioRxiv - Microbiology 2021Quote: ... The centrifugation step was repeated and the pellet air-dried before the DNA was dissolved in 10 mM Tris-HCl (pH 8.5, Qiagen) at 65 °C for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... adjusted to pH 8.0 using 1M Tris-HCl pH 8.0 and incubated at room temp for 1 h with Ni-NTA Agarose beads (Qiagen). Beads were washed twice with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was lysed with a lysis buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 20 mM EDTA, 1% SDS) and proteinase K (Qiagen) was added to samples to degrade proteins ...
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bioRxiv - Biochemistry 2023Quote: ... 5-6 mg of frozen tissue was homogenized in 250 μL ice-cold acidified methanol (0.1% HCl) and 250 μL water washed with hexanes in a bullet blender (TissueLyser II, Qiagen) with 5 mm stainless steel beads (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... leaf tissues were collected and homogenized in 400 μL of TPS buffer (100 mM Tris-HCl, 10 mM EDTA, 1 M KCl, pH8.0) using TissueLyser II (Qiagen), followed by incubation for 20 min at 75°C ...
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bioRxiv - Immunology 2023Quote: ... 40mM Tris-HCl ph 6.5 for 2 hour at 45°C prior to purification with QIAquick PCR Purification Kit (Qiagen). For ‘input’ DNA ...
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bioRxiv - Biochemistry 2022Quote: ... The reactions were neutralized with 0.7 µL 25% HCl and nucleotide removal was performed with the QIAquick nucleotide removal kit (QIAGEN). The samples were dried under vacuum for 2 hours at 60°C for subsequent gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were neutralized with 0.7 μl of a 7.5 M HCl stock and the remaining complementary DNA was purified using a nucleotide removal kit (QIAGEN). Sequencing reactions were performed using a commercial kit designed to be used with fluorescent dye-labeled primers (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... 200ul and 600ul Buffer RLT (containing 1% β-mercaptoethanol, QIAGEN RNeasy Mini Kit) was added to nuclear and cytosolic fractions respectively and vortexed vigorously ...
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bioRxiv - Immunology 2021Quote: ... with 10 μL/mL β-mercaptoethanol and mechanically disrupted with a TissueRuptor (Qiagen), before RNA extraction ...