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Citations for Qiagen :
401 - 450 of 5664 citations for Primary Human Retinal Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from primary solution with the removal of genomic DNA by the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). The purity and concentration of extrasted totel RNA were confirmed using a NanoDrop ONE spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... primary prostate tumor tissue cores (three 20 µm-thick, unstained FFPE sections per patient) using the Rneasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The human U6 primer used in this experiment was included in the miScript Primer assay kit (Qiagen Inc., Germantown, MD, #218300).
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bioRxiv - Molecular Biology 2022Quote: ... FAF1 (Uniprot identifier Q9UNN5-1) and UBXN7 (Uniprot identifier O94888-1) were amplified from XpressRef Universal Total human RNA (QIAGEN, 338112) by RT-PCR (TaKaRa ...
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bioRxiv - Developmental Biology 2021Quote: RNA was isolated from a 23 week human fetal heart using Trizol-based dissociation followed by the RNEasy Mini Kit (Qiagen #74104). cDNA was created from this RNA using the iScript Reverse Transcription Supermix (Bio-Rad #1708840) ...
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bioRxiv - Systems Biology 2022Quote: DNA was isolated from human buffy coat samples at the Crimson Core facility (Mass General Brigham) using the QIAamp DNA Blood Mini Kit (Qiagen 69504) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Reference plasmids were serially diluted in either PBS buffer or heat-inactivated normal human serum (NHS) and re-extracted using the QIAamp® DNA Blood Mini Kit (Qiagen) per recommended protocol for serum ...
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bioRxiv - Cancer Biology 2020Quote: Flag-tagged NF1-GRD (aminoacids 1131-1534) was amplified by PCR from human cortical tissue (epilepsy patient) and first cloned in the pDRIVE vector (Qiagen #231124). Primers are listed in Supplementary Table 5 ...
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bioRxiv - Immunology 2021Quote: ... and ImmunoCult™ Human Treg Differentiation Supplement (StemCell TechnoRNA was isolated at set time points after activation using the RNeasy Mini (Qiagen). cDNA was generated using the High-Capacity RNA-to-cDNA Kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Exosomal miRNAs were profiled using Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, #YAHS-106Y, Plate Format: 2 × 96-well).
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bioRxiv - Microbiology 2023Quote: RNA was extracted from a SARS-CoV-2 Omicron BA.1 clinical isolate (SARS-CoV-2/human/USA/CA-CDC-4358237-001/2021) (GenBank: OM264909.1) using the QIAamp Viral RNA kit (Qiagen, Hilden, Germany). The complete wild-type Omicron genome was then reverse transcribed via RT-PCR with SuperScript™ IV First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of RNA isolated from human islets or 10 µL of exosomal RNA was reverse transcribed using the miRScript II kit (Qiagen, Germany), following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The association of the differentially expressed proteins with specifically dysregulated regulatory/metabolic networks in OT human samples was analyzed using QIAGEN’s Ingenuity Pathway Analysis (IPA; QIAGEN Redwood City). This software calculates significance values (p-values ...
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... Cells were resuspended in RNAprotect Cell Reagent (Qiagen) and stored at -80°C prior to extracting RNA with RNeasy RNA extraction kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were collected in RNAprotect Cell Reagent (Qiagen), and total RNA was extracted using the RNeasy Plus Micro kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then stained by Coomassie-blue or immunodetected as described before [26] using primary polyclonal antibodies directed against His6 epitope-tag (Penta His, Qiagen, dilution 1:1000), V5 epitope-tag (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... total RNA from magnetically purified human NDC, PSEN1M146L, and PSEN1A246E (replicates, n = 4) hiPSC-derived neurons was prepared using RNeasy Plus Micro Kit (Qiagen Cat. 74034) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, and PSEN1A246E (replicates, n = 3) human iPSC-derived neurons was prepared using miRNeasy Micro Kit (Qiagen Cat. 217084), based on manufacturer’s procedures ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, PSEN1A246E, and PSEN1H163R (replicates, n = 3) human iPSC-derived neurons was prepared using RNeasy Plus Micro Kit (Qiagen, Cat. 74034) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... we extracted whole blood DNA from all individuals included in the RNA sequencing data using Gentra® Puregene® for human whole blood kit (QIAGEN) and MagAttract® HMW DNA kit (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out by using the ‘Human Innate and Adaptive immune Response’ kit (Qiagen, Hilden, Germany) to assess the expression of genes/mRNA ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 1 mL of each human plasma sample using the DNeasy Blood and Tissue kit (Qiagen, 69504, Hilden, Germany) according to the manufacturer protocol and recommendations ...
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bioRxiv - Microbiology 2023Quote: Microbiome DNAs were extracted from the human samples on the same day of collection using the microbiome-specific kit (QIAamp DNA Microbiome Kit; Qiagen, Hilden, Germany). The DNA extraction was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Subsequent targeted sequencing of 160 cancer-related genes was performed using a QIAseq Human Comprehensive Cancer Panel v2 (Qiagen Inc, Valencia, CA) (Table S2) ...
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bioRxiv - Cell Biology 2023Quote: ... Key genes involved in the regulation and enzymatic pathways of fatty liver were simultaneously assayed with the RT2 Profiler PCR Array Human Fatty Liver (PAHS-157ZC-6) (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions and analyzed with the Data Analysis Center (QIAGEN Hilden ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1 ng of RNA from microdissected human pancreatic islets and from EndoC-βH1 was used to generate cDNA libraries using QiaSeq miRNA library kit (Qiagen, Hilden, Germany) following manufacturer’s instructions (see ESM Methods).
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bioRxiv - Physiology 2024Quote: Total RNA was isolated from 50 mg of frozen human myocardial LV tissue samples using the miRNeasy Mini Kit (Qiagen: Hilden, DE). To remove all DNA ...
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bioRxiv - Neuroscience 2024Quote: The expression of 84 PPAR target genes was assessed using the Human PPAR Targets RT2 Profiler PCR Array (Qiagen; 384-well format) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Red blood cells were lysed by treating the cells with Red Blood Cell lysis buffer (Qiagen) for 5 min ...
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bioRxiv - Immunology 2022Quote: RNA was isolated from Apoe-KO and Apoe/Has3-DKO aortae and primary bone marrow derived monocytes by using the RNeasy® Plus Universal Kit Mini 50 (Qiagen GmbH, Hilden, Germany). RNA concentration and purity (260 nm/ 280nm ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were FACS-sorted for mCherry+ cells directly into RNA-stabilizing cell lysis buffer (Qiagen Buffer RLT). Homogenization was performed on QiaShredder columns ...
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bioRxiv - Immunology 2022Quote: ... DNA was extracted from frozen cell pellets (3×107 cells; Blood & Cell Culture DNA Maxi Kit, Qiagen) per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Genomic DNA from cells in Qiagen Cell Lysis were purified using Qiagen Puregene Cell Kit (Qiagen 158722) according to manufacturer’s instructions and resuspended in 100µl nuclease-free H20 ...
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bioRxiv - Cancer Biology 2024Quote: ... To profile the expression of genes related to p53-mediated signal transduction based on the RT² Profiler™ PCR Array Human p53 Signaling Pathway (#330231, PAHS-027ZC, Qiagen, Hilden, Germany), reverse transcription and qPCR were performed using the RT2 First Strand Kit (#330401 ...
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bioRxiv - Immunology 2020Quote: Cells were resuspended and stored in RNAprotect cell reagent (Qiagen). RNA was extracted with the RNeasy mini kit (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... Single-cell suspensions were depleted of red blood cells (Qiagen) before downstream use.
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (Qiagen) to generate the protein expression strains ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Qiagen). The bacterial cultures were grown in LB medium with 100 μg/ml ampicillin and 50 μg/ml kanamycin at 37°C to an OD600 nm of 0.8 and expression was induced with 1 mM isopropylthio β-D-1-galactopyranoside (IPTG) ...
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bioRxiv - Immunology 2019Quote: ... Red blood cells were removed by treating the cells with 5 ml Red Blood Cell Lysis buffer (Qiagen) for 4 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell fractionation was performed with the Qproteome Cell Compartment kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: Cell pellets were resuspended in 300 μL cell lysis solution (Qiagen) and lysed by incubation at 50°C for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... High titre baculovirus was produced by transfecting bacmid DNA into Sf21 cells at 0.5 × 106 cells/ ml cells using Superfect (Qiagen) in 24 deep-well blocks ...
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bioRxiv - Cell Biology 2021Quote: Isolated Tert+ and Tert-cells (≤ 5 cells) were subjected to synthesize the complementary DNA (cDNA) using REPLI-g WTA Single Cell Kit (QIAGEN) and analyzed for gene expression by qRT-PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... confluent 293T-REx cells (25 cm2) were harvested and nuclei were isolated from 5×106 cells with the Qproteome Cell Compartment Kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: 3 million cells from cell lines or patient samples were pelleted and resuspended in 1 mL of Cell Lysis Solution (Qiagen) mixed with 500 μg of RNase A ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 integration was examined in 293T cells and J-Lat cells by isolating genomic DNA from 1 × 106 cells using the DNeasy Tissue Kit (Qiagen). Alu-LTR sequences were amplified during first round PCR from 100 ng of total genomic DNA using the following primers ...
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bioRxiv - Immunology 2022Quote: ... for cell fractions ≥0.2×106 cells and the RNeasy Micro Kit (Qiagen) for fractions ≤0.2×106 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Cell culture samples were collected with RNAprotect Cell Reagent (Qiagen, Germantown, MD) and the mRNA was extracted using a Qiagen RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... Red blood cells were lysed using Red Blood Cell Lysis buffer (Qiagen) for 1 min ...
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bioRxiv - Genetics 2020Quote: Cell pellets were resuspended in 600 ul Cell Lysis Solution (Qiagen, 158906) supplemented with 3 ul Proteinase K (NEB ...