Labshake search
Citations for Qiagen :
4351 - 4400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Plasmid DNA was prepared (Qiagen, 27104) and sequenced from T7 primer by Sanger sequencing.
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bioRxiv - Cell Biology 2024Quote: ... p.H 8.0 (Qiagen 12162) to a total volume of 250µl ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the RNeasy mini kit (Qiagen). Library preparation was carried out by the Edinburgh Clinical Research Facility from 500 ng of each RNA sample using the NEBNext Ultra II Directional RNA library kit with PolyA enrichment module (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... The DNAs were purified using the MinElute PCR Purification Kit (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... cut out from the gel and purified using QIAquick gel extraction kit (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... cut out from the gel with blade and tweezer and purified using QIAquick gel extraction kit (Qiagen). ° PAUSE POINT PCR fragments can be stored at −20 °C up to several months
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bioRxiv - Developmental Biology 2024Quote: ... and QIAcube (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted using the DNeasy blood and tissue kit (69506; Qiagen). Exon 3 of Smad4 was amplified by PCR using the Q5 High Fidelity DNA polymerase (M0491S ...
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bioRxiv - Microbiology 2024Quote: ... The ribosomal pellet was resuspended in 700 μl of Qiazol reagent from the miRNeasy kit (Qiagen; #217004). The mixture was flash-frozen in liquid N2 and stored at −80°C until RNA extraction for Ribo-seq.
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bioRxiv - Microbiology 2024Quote: ... 200 μl of lysis buffer-faecal sample mixture was added with 800 μl of InhibitEX buffer of the QIAamp DNA Stool Mini Kit (Qiagen GmbH, Hilden, Germany). Next ...
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IL-6 SIGNALING EXACERBATES HALLMARKS OF CHRONIC TENDON DISEASE BY STIMULATING REPARATIVE FIBROBLASTSbioRxiv - Bioengineering 2024Quote: ... The frozen samples were pulverized by cryogenic grinding (FreezerMill 6870, SPEXTMSamplePrep) and further processed with the RNeasy micro kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: miRNAs were extracted from serum (miRNeasy Serum/Plasma Advanced kit QIAGEN, Hilden, Germany) with the spike-in control cel-miR-39-3p (QIAGEN ...
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bioRxiv - Systems Biology 2024Quote: ... were transfected with one of three different commercial siRNA sequences designed to target the EGFR or an Allstars negative control siRNA sequence (final concentration 1 nM; all Qiagen Ltd., UK) using INTERFERin transfection reagent according to the manufacturer’s instructions (Polyplus Transfection ...
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bioRxiv - Biochemistry 2024Quote: ... plates were quickly washed 2X with ice cold PBS and then total RNA was extracted following manufacturer’s protocol (RNeasy Plus Minikit (Qiagen, 74134) and RNA concentration and purity was assessed (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from cells using RNeasy Plus Mini Kit (Qiagen #74134) following manufacturer’s instructions and re-suspended in nuclease-free water ...
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bioRxiv - Molecular Biology 2024Quote: ... and analysed using a One Step RT-PCR kit (Qiagen, #210210) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCR products were examined using the QIAxcel® capillary electrophoresis system 100 (Qiagen). Statistical analyses were performed using GraphPad Prism 9.5.0.
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were analysed and quantified using the QIAxcel® capillary electrophoresis system 100 (Qiagen).
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... Cells were transduced at 70% confluency using Effectene transfection kit (QIAGEN) and imaged after 24 – 48 hours ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... HEK293T cells were transfected with Effectene transfection kit (QIAGEN). 24 hours after transfection cells were disscociated with Versene (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... No siRNAs were added to mock samples and 50 pmol of scrambled siRNA (SI03650318; QIAGEN) was used as a negative control ...
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bioRxiv - Molecular Biology 2024Quote: Whole cell RNA was extracted from snap frozen liver (miRNeasy mini kit QIAGEN, Hilden, Germany) and reverse transcribed (QuantiTect Reverse Transcription kit QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... Samples were treated using the MinElute PCR Purification Kit (QIAGEN, Hilden, Nordrhein-Westfalen, Germany). The products were quantified using a Qubit dsDNA BR Assay Kit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: Viral RNA was extracted from 50 ml cell culture medium using QIAamp Viral RNA Mini Kit according to protocol provided by the manufacturer (Qiagen; Cat. no. 52904). RNA concentration and quality were evaluated using NanoDrop 2000 Spectrophotometer (Thermo Fischer Scientific) ...
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bioRxiv - Microbiology 2024Quote: We used the QIamp DNA Mini Kit (QIAGEN, Hilden, Nordrhein-Westfalen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... We assembled qPCR reactions with QuantiFast SYBR Green RT-PCR Kit (Qiagen #204156), gene-specific oligonucleotides (Supplementary Table 14 ...
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bioRxiv - Biophysics 2024Quote: ... we used the RNeasy Mini Kit (Qiagen #74104), the QIAshredder (Qiagen #79654 ...
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bioRxiv - Biophysics 2024Quote: ... the QIAshredder (Qiagen #79654) and the DNase I (Qiagen #79254 ...
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bioRxiv - Biochemistry 2024Quote: ... The media was incubated with pre-equilibrated Ni-NTA beads (Qiagen) at room temperature for 1-2 hours (1 ml beads/200 ml media) ...
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bioRxiv - Cancer Biology 2024Quote: ... with the plasmid as per instructions and isolated by QIAGEN HiSpeed Plasmid Midi Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2024Quote: ... and primary cells using Rneasy Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: The DNA/RNA samples were isolated using either the AllPrep DNA/RNA Mini Kit or the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: RNA samples were harvested from cells using the RNeasy kit Mini (Qiagen, Cat#217004, Germantown, MD, USA). The concentration was determined using the NanoDrop ND-1000 Spectrophotometer ...
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bioRxiv - Bioengineering 2024Quote: ... The EndoFree Plasmid Maxi Kit (Qiagen) was used to prepare DNA for transfection.
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were extracted from 1 million cells using the RNeasy Mini Kit (Qiagen, 74136) and treated with TURBO DNA-freeTM Kit (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... and either Mini-prepped (using a Qiagen Mini-prep kit) or Midi-prepped (using a Macharey-Nagel NucleoBond Xtra Midi kit ...
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bioRxiv - Synthetic Biology 2024Quote: ... The supernatant was applied to a Ni-NTA agarose drip column (Qiagen) and eluted with buffer A supplemented with 250 mM imidazole ...
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bioRxiv - Neuroscience 2024Quote: ... These samples were subjected to RNA extraction using the RNeasy Plus Mini kit (Qiagen, 74136). Library preparation (polyA-selected ...
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bioRxiv - Biochemistry 2024Quote: ... purified using the Qiaquick gel extraction kit (Qiagen), and cloned into linearized pUC19 by In-Fusion cloning (Takara Bio) ...
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bioRxiv - Molecular Biology 2024Quote: Whole cell RNA was extracted (miRNeasy mini kit QIAGEN, Hilden, Germany) and reverse transcribed (SuperScript VILO cDNA synthesis kit Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Ingenuity Pathway Analysis (IPA, Qiagen) was employed for canonical pathway and graphical pathway analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... with the spike-in control cel-miR-39-3p (QIAGEN, Hilden, Germany) and reverse transcribed (qScript microRNA cDNA synthesis kit Quanta Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... The DNeasy Blood and Tissue Kit (QIAGEN) was used for the DNA isolation ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was isolated from MYOrganoids by RNeasy micro kit (QIAGEN) accordingly to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Cultures before and after selection were spun down and we lysed the cells and performed a standard plasmid extraction protocol using QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany). Illumina amplicons were generated by PCR of the barcode region ...
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bioRxiv - Bioengineering 2024Quote: ... Mechanical disruption was performed using the TissueLyser II and 8-mm stainless steel beads (both Qiagen) at an amplitude of 30 for 2 min at a time ...
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bioRxiv - Bioengineering 2024Quote: ... using the HotStarTaq Plus Master Mix Kit (Qiagen) as follows ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified by affinity chromatography using Ni-NTA agarose (Qiagen) resin followed by his tag cleavage by TEV protease and gel filtration using Superdex 200 Increase 10/300 GL column (G.E ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted using the Genomic tip 20/G kit (Qiagen) per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... corresponding to ∼5 L per sample (RNeasy PowerSoil Total RNA Kit; Qiagen). The RNA was tested with Bioanalyzer ...