Labshake search
Citations for Qiagen :
4151 - 4200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... and column-purified (QIAquick PCR purification kit, QIAGEN) for Gibson assembly ...
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bioRxiv - Biophysics 2024Quote: ... The reactions were column-purified (QIAquick PCR purification kit, QIAGEN), and 40 ng DNA were used as template for a PCR2 reaction with the standard i5 and i7 primers to add the remainder of the Illumina adapter sequences and the demultiplexing indices (dual indexing ...
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bioRxiv - Cancer Biology 2024Quote: Total RNAs were extracted with the RNeasy Plus Mini kit (#74136, Qiagen). RNA quality was assessed using the DNF-471 RNA kit on a fragment analyzer (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from cells using the RNeasy Mini Kit (Qiagen, Cat #74104) and tissue using TRIzol (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... using RNeasy Mini kit (QIAGEN #74104), total RNA was extracted from HAECs ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesised using 1µg RNA as template using Quantitect Reverse Transcription Kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and 20 mM imidazole.[50,51] The cells were disrupted via probe sonication (Brandson) and the protein was purified with Ni-NTA affinity chromatography (Qiagen). The nanopores were eluted from the beads with 3× 250 µL fractions of elution buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was isolated from puromycin-resistant hiPSC colonies using the QIAamp DNA Blood Mini kit (Qiagen, #51104). To ensure correct incorporation of the RUNX1 HDR template into the ETV6 locus ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted with the RNeasy Mini kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed using x1 SyBr green (Qiagen), 1:10 dilution of cDNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... Ingenuity pathway analysis (IPA, Qiagen) of REH cells was performed taking into account significantly dysregulated genes (absolute fold change >1.5 and p<0.05) ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was done according to the manufacturer’s instructions (Qiagen, Hilden, Germany).
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted by the RNeasy Mini Kit (QIAGEN, Cat#70106). cDNA synthesis was performed using the Luna script RT Supermix kit (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... FACS-sorted cultures and 1cm2 dorsal skin samples using an RNEasy Micro Kit (Qiagen) for FACS-sorted cultures and a Direct-zol RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Cell Biology 2024Quote: The gDNA was extracted using the DNeasy blood and tissue genomic DNA extraction kit from Qiagen® ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was extracted from hiPSC lines using the DNeasy Blood & Tissue Kits (Qiagen). The DNA quality was assessed using the TapeStation Agilent Genomic DNA ScreenTape Assay (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... and Effectene transfection agent (Qiagen, Germantown, MD) as described previously [48] ...
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bioRxiv - Cancer Biology 2024Quote: ... Final samples of 3 or 30 million cells were collected and genomic DNA was extracted (DNeasy blood and Tissue kit, Qiagen). Next ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was extracted six hours after transformation using QIAGEN RNeasy Maxi Kit (QIAGEN cat. 75162) following manufacturer’s manual ...
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bioRxiv - Bioengineering 2024Quote: ... Real-time qPCR were run on a Rotor-Gene Q thermocycler from QIAGEN using primer pairs (1R69,1R70) ...
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bioRxiv - Biophysics 2024Quote: ... RNA was purified using RNEasy kit (Qiagen) and stored as stocks and dilutions in RNase-free water at −80 °C ...
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bioRxiv - Biophysics 2024Quote: ... the supernatant was first purified buy a Ni-NTA (Qiagen) column since the LRRK2 construct includes cleavable His6-ZZ tags ...
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bioRxiv - Cancer Biology 2024Quote: ... The optional step to remove genomic DNA using RNase-free DNase (Qiagen) was also included for all samples ...
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bioRxiv - Cancer Biology 2024Quote: ... and the resulting powdered tissue was resuspended in RLT-buffer (Qiagen RNeasy Mini Kit) and then further homogenized using Precellys tubes and Precellys Evolution Homogenizer (Bertin Instruments) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was then extracted using the RNeasy Mini kit (Qiagen) including an on-column DNase digest (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... and A498 cells infected with lentivirus expressing shRNA targeting the indicated transcripts was isolated using RNeasy Mini Kit (QIAGEN #74104) and QIAshredder (QIAGEN #79654) ...
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bioRxiv - Cancer Biology 2024Quote: ... 50ng of RNA was reverse transcribed into cDNA using the miRCURY LNA RT kit (Qiagen). RT-qPCR mix was prepared using the miRCURY LNA SYBR Green PCR kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: DNA from TIL samples was isolated using QIAamp DNA Blood Mini Kit (QIAGEN). DNA from FFPE samples was isolated using RNA/DNA Purification Plus Kit (Norgen Biotek ...
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bioRxiv - Cancer Biology 2024Quote: Blood buffy coat RNA was purified from frozen buffy coat samples using miRNeasy Mini Kit (QIAGEN). Approximately 150 mm3 of frozen blood was cut out of a cryogenic tube using a scalpel and placed in a 2 mL Eppendorf tube ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor DNA was purified from fresh-frozen tumors or formalin-fixed paraffin-embedded tumors using Gentra Puregene Tissue Kit (QIAGEN) or AllPrep DNA/RNA Kit (QIAGEN) ...
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bioRxiv - Cancer Biology 2024Quote: ... or AllPrep DNA/RNA Kit (QIAGEN), respectively.
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bioRxiv - Cancer Biology 2024Quote: ... followed by total RNA isolation using TRIzol™ Reagent (#15596018, Qiagen). cDNA was generated from 1 µg of total RNA via an iScript™ cDNA Synthesis Kit (#1708891 ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR mix was prepared using the miRCURY LNA SYBR Green PCR kit (Qiagen). qPCR was performed using the QuantStudio 7 Flex System ...
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bioRxiv - Cancer Biology 2024Quote: ... (Qiagen, cat no: 74034). RNA concentration was measured using Nanodrop (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted using RNeasy kit (Qiagen, Germantown, MD) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and total RNA was extracted using RNeasy Mini Kit (Qiagen #74104). One µg of RNA was used to generate cDNA with QuantiTect Reverse Transcription kit (Qiagen #205313) ...
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bioRxiv - Cell Biology 2024Quote: ... and then cleaned up using the MiniElute reaction clean up kit (Qiagen). Tagmented DNA was amplified with barcoded primers ...
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bioRxiv - Cancer Biology 2024Quote: ... disrupted and homogenised with TissueRuptor II (Qiagen) before being lysed with a SDS lysis buffer supplemented with protease inhibitor ...
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bioRxiv - Cancer Biology 2024Quote: Using QIAGEN Blood and Cell Culture DNA kits (Qiagen, Germany), total DNA from cell pellets was extracted in accordance with the manufacturer’s instructions with a few minor adjustments to preserve mtDNA ...
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bioRxiv - Cell Biology 2024Quote: ... pathway enrichment analyses were done by knowledge-based Ingenuity Pathway Analysis (IPA; Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was extracted using the Blood & Cell Culture DNA Kit Midi (Qiagen) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was isolated from cells using DNeasy Blood and Tissue Kit protocol (QIAGEN). DNA concentration was determined using NanoDrop 2000 spectrophotometer and diluted to equal concentrations ...
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bioRxiv - Cancer Biology 2024Quote: ... and PRKACA (GS5566) were purchased from Qiagen (Hilden, DEU). Cells were transfected with Lipofectamine RNAiMAX transfection reagent (#13778500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA (1 μg) was reverse transcribed using the Quantitect Reverse Transcription Kit (Qiagen). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted for total RNA sequencing using the Direct-zol RNA Miniprep Kit (ZymoResearch R2050) or the miRneasy Mini Kit (Qiagen 217004) according to manufacturer protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted using the miRNeasy kit with DNase I digestion (Qiagen 217004 and 79254), and cDNA synthesis was performed with iScript™ Reverse Transcription Supermix (Bio-Rad 1708841) ...
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bioRxiv - Cancer Biology 2024Quote: PBMCs were prepared for DNA extraction using the QIAamp DNA Mini and Blood Mini (QIAGEN, 51104). The PBMC sample was mixed with QIAGEN Protease and Buffer AL and then incubated at 56°C to release DNA ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA from cell lines was extracted as per the manufacturer’s instructions (RNeasy Mini Kit, QIAGEN). Total RNA (1 μg ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was purified with a Qiagen MinElute PCR Purification Kit (Qiagen, #28004). Following purification ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA from ear notches was isolated using the DNeasy Blood and Tissue kit (Qiagen) and amplified using PCR with HotStar Taq (Qiagen) ...