Labshake search
Citations for Qiagen :
4101 - 4150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Tissues were directly lysed in ice-cold RNA isolater (Vazyme, R401) for total RNA isolation using RNeasy Mini Kit (Qiagen, 74004). Each group contains three replicates ...
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bioRxiv - Cell Biology 2024Quote: ... in QIAzol solution (#79306, Qiagen). To avoid genomic DNA contamination ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection was conducted with 5 μg plasmids (act-GAL4, UAS-lbm-GFP, UAS-upd2-mCherry) using Effectene Transfection Reagent (QIAGEN, #301425).
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bioRxiv - Immunology 2024Quote: Samples were double-sorted using a FACSAria or FACS Astrios: 600-1000 cells from each population were collected into 5 μl TCL buffer (Qiagen) containing 1% beta-mercaptoethanol (β-ME ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was amplified with a QuantiTect SYBR Green PCR kit (QIAGEN) and a Rotor-Gene RG-3000 (Corbett Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCRs were performed with a QuantiTect SYBR Green PCR kit (QIAGEN) and a Rotor-Gene RG-3000 (Corbett Research) ...
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bioRxiv - Pathology 2024Quote: ... 4 sections 10 µm thick were placed in a chilled Eppendorf tube and the RNA was extracted using a protocol from Qiagen (Rneasy FFPE Kit 73504), following extraction ...
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bioRxiv - Plant Biology 2024Quote: RNA was isolated with the QIAGEN RNeasy kit and Total RNA was treated with RNase-free DNase I set (QIAGEN). RNAseq libraries were prepared and up to 20 million 150bp paired-end sequences were collected by Illumina-sequencing by Novogene (Uk) ...
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bioRxiv - Plant Biology 2024Quote: ... The tip-end was capped with a 20-μm polypropylene frits disk (Aglient) and then packed with Ni-NTA silica resin (QIAGEN, Hilden, Germany) by centrifugation at 200 ×g for 1 min ...
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bioRxiv - Plant Biology 2024Quote: ... Tissue samples were ground by shaking with glass beads in a TissueLyser II (Qiagen, 2 pulses of 30 s each at 28Hz shaking). Leaf powder was suspended in 1.2 ml of cold nuclear isolation buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... Gene expression was quantified using the QuantiTect SYBR Green RT-PCR Kit (Qiagen, Germany) in Bio-Rad CFX96 Real-time PCR detection system ...
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bioRxiv - Plant Biology 2024Quote: ... qPCR analyses were performed with a QuantiFast SYBR Green PCR kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol using specific primers for petD and psbA as in a previous work (Krupinska et al. ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted using an RNeasy Plant Mini Kit (Qiagen, Tokyo, Japan). For RNA-seq library preparation ...
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bioRxiv - Plant Biology 2024Quote: ... Fragments were gel purified using QIAquick Gel Extraction Kit (QIAGEN, UK) and assembled into the backbone (Kpn1 and BamH1 digested ...
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bioRxiv - Plant Biology 2024Quote: ... DNase treatment was made by adding 40 µL of DNase solution mix (10μl DNase I Stock solution + 30μl Buffer RDD; RNase-Free DNase Set, Qiagen) to each column ...
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bioRxiv - Plant Biology 2024Quote: ... and residual DNA was removed with the RNase-Free DNase Kit (Qiagen). A Qubit 2.0 Fluorometer was used to calculate RNA concentration and 4 mg of total RNA was used to prepare libraries with the TruSeq Stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) and residual DNA was removed with the RNase-Free DNase Kit (Qiagen) ...
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bioRxiv - Pathology 2024Quote: Cultured SAECs were washed with PBS and total RNA from the samples were extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... PCR products were run ona 3% agarose gel to recover of the NGS amplicon libraries and purified by MinElute Gel Extraction Kit (QIAGEN, Germantown, MA). Libraries were sequenced on an Illumina Novaseq 6000 using a 350-cycle reagent kit and paired end read chemistry yielding 175 bp paired end read outputs ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA (gDNA) or RNA from the tissues was isolated using a QIAamp DNA mini kit or RNeasy Plus kit (QIAGEN, Germantown, MA). Sequence encoding the modified capsid insertion was amplified using serotype-specific primers with PCR cycles less than 30 by using Q5 2x Master Mix (NEB ...
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bioRxiv - Plant Biology 2024Quote: We isolated total RNA isolated from plant shoots using the RNeasy Plant Mini kit (Qiagen, Germany) and treated with DNA-Free kit (Thermo Fischer Scientific ...
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bioRxiv - Plant Biology 2024Quote: We treated the metatranscriptome total RNA with the FastSelect 5S/16S/23S reagent (Qiagen, cat# 335921) to deplete the rRNA following the manufacturer protocol ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted using RNeasy (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The EVs were further purified using exoEasy Maxi kit (Qiagen). The resulting samples ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, USA). Pollen tubes were isolated by centrifugation at 3000 rpm ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using the “RNeasy Plant Mini Kit” (Qiagen Sciences, Germantown, MD, USA) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... and M15/pREP4 (Qiagen) strains were used for protein expression ...
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bioRxiv - Molecular Biology 2024Quote: Gcn4 and Med15 supernatants were mixed with 5 ml Ni-NTA beads (Qiagen) and incubated at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... reverse transcription to cDNA (Qiagen quantitect reverse transcription kit- cat# 205311 ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was isolated using a DNA mini kit (Qiagen). Subsequently ...
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bioRxiv - Microbiology 2024Quote: The QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) was used to perform RNA extractions ...
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bioRxiv - Biophysics 2024Quote: ... and the supernatant was incubated with Ni-NTA agarose (QIAGEN) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and cleaned with QIAGEN MinElute PCR purification kit (QIAGEN cat. 28006). The library was inserted into the vector using In-Fusion cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... we employed Ingenuity Pathway Analysis (IPA, QIAGEN)24 ...
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bioRxiv - Cancer Biology 2024Quote: The Rneasy Plus Micro Kit (Qiagen, Valencia, CA) was used for RNA extraction ...
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bioRxiv - Bioengineering 2024Quote: ... and the RNeasy® Micro Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... the RNeasy Mini Kit (Qiagen) was implemented according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cleaned up with a PCR purification kit (#28104, Qiagen) and ligated into the cut LT3GEPIR vector with T4 DNA Ligase at a 3:1 insert ...
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bioRxiv - Cancer Biology 2024Quote: ... WT mouse ovaries and AT29 cells allografts were lysed with TissueLyser (Qiagen) in Frackelton buffer ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then applied to a Ni-NTA agarose gravity column (Qiagen). The column was washed with Lysis Buffer B ...
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bioRxiv - Biophysics 2024Quote: ... membranes were incubated under soft shaking with Penta-His antibody (Penta His HRP Conjugate; Qiagen) for 1 h with 0.5 % (w/v ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was loaded onto a Ni-NTA (Qiagen) column ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated with RNeasy Mini Kit (74104, QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA was purified from the samples using the AllPrep DNA/RNA/miRNA Universal Kit (80224, QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated using the Qiagen RNeasy Mini kit (Qiagen, 74104) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... liver-metastasis and adjacent normal tissue were lysed in RIPA buffer using the Tissuelyser LT (Qiagen). Total protein content was determined using DC protein assay (Bio-rad ...
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bioRxiv - Cell Biology 2024Quote: ... gDNA was collected from the cells transfected with px459 using the DNeasy kit (Qiagen), and PCR amplification followed by T7 endonuclease digestion confirmed that the guides cut efficiently ...
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bioRxiv - Cell Biology 2024Quote: RNA extraction and purification was performed using the RNeasy mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were collected in RLT Plus Buffer (Qiagen RNeasy Plus Micro Kit ...
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bioRxiv - Cell Biology 2024Quote: ... Stable transfection of indicated vectors into S2 cells was performed using Effectene Transfection Reagent (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...