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Citations for Qiagen :
351 - 400 of 1923 citations for Human DOK4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... The HiSpeed Plasmid Maxi Kit (Qiagen) was used to generate larger amount of the FLAG-KRAS plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid was transfected using Effectene (Qiagen 301427 ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were isolated by miniprep (Qiagen) and correct spacer sequences were verified by Sanger sequencing using primer ONV92.
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bioRxiv - Microbiology 2023Quote: ... or QIAGEN Plasmid Midi kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... using the Plasmid Maxi Kit (QIAGEN). The pDNA was digested using BsmBI-v2 restriction endonuclease (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... and QIAprep plasmid Miniprep kit (Qiagen) were used for the purification of PCR products and plasmid DNA respectively ...
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bioRxiv - Synthetic Biology 2023Quote: ... a plasmid DNA miniprep (Qiagen, 27106), and Sanger sequencing (Azenta ...
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bioRxiv - Microbiology 2023Quote: pQE expression plasmids (Qiagen, Manchester, UK) containing DNA encoding species D fiber knob proteins were produced by TWIST Biosciences (San Francisco ...
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bioRxiv - Genomics 2023Quote: After plasmid extraction (QIAGEN No.27106) and sequencing validation ...
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bioRxiv - Cancer Biology 2024Quote: ... with the plasmid as per instructions and isolated by QIAGEN HiSpeed Plasmid Midi Kit (QIAGEN, 12643). LucOS-Blast vector was obtained from Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmid DNA was prepared (Qiagen, 27104) and sequenced from T7 primer by Sanger sequencing.
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bioRxiv - Bioengineering 2024Quote: ... The EndoFree Plasmid Maxi Kit (Qiagen) was used to prepare DNA for transfection.
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bioRxiv - Neuroscience 2024Quote: ... and Plasmid Miniprep Kit (27106, Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... All plasmids were purified (Qiagen, #12943) and sequence validated through long-read sequencing (SNPsaurus) ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA for ANXA2 (Human, Qiagen, Hs_ANXA2_8, SI02632385) or siRNA for Anxa2 (Mouse ...
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bioRxiv - Bioengineering 2019Quote: ... The plasmid DNA was extracted using the Qiagen QiaFilter Plasmid Midi and Maxi Kit (Qiagen, Germantown, MD) and linearized by restriction enzyme digestion to increase the efficiency of chromosomal integration into Jurkat cells ...
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bioRxiv - Developmental Biology 2019Quote: ... All the three plasmid-sgRNA combinations plus empty plasmids were transfected to hek293T cells with SuperFect (QIAgen) following the fabricant’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... All the plasmids for AAV vector preparations were made using endotoxin free plasmid maxiprep kit (#12663, Qiagen). AAV 293 cells (#CVCL_6871 ...
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bioRxiv - Microbiology 2022Quote: ... The colonies were harvested and the plasmids were extracted by Midi prep (HiSpeed Plasmid Midi Kit, QIAGEN).
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bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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bioRxiv - Neuroscience 2020Quote: ... Amplification and purification of the different plasmids were performed using the Plasmid Midi kit (Qiagen, Hilden, Germany). Production was carried out by transfecting HEK293-T cells in 10 cm dishes (2×106 cells/dish ...
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bioRxiv - Cell Biology 2019Quote: ... pMD2G and pWPTS-HPSE2 plasmids were purified using an EndoFree® Plasmid Maxi kit (Qiagen, Valencia, CA), and co-transfected (using ratio pWPTS-HPSE2:pCMV-dR8.74:pMD2G = 3:2:1 ...
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bioRxiv - Biophysics 2022Quote: ... coli colonies were collected with scrapers and the library plasmids were purified using Plasmid Maxi Kit (Qiagen). Lentiviral libraries were prepared as described above in complete DMEM and harvested using PEG-it Virus Precipitation Solution (System Biosciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... a control plasmid containing the FKBP12F36V insert was purified from a bacterial clone (QIAGEN Plasmid Maxi kit). Six serial dilutions of the plasmid were generated ...
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bioRxiv - Immunology 2023Quote: ... Plasmids with the cloning of synthesized antibody genes were prepared using the Megaprep plasmid plus kit (Qiagen) for transient transfection ...
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bioRxiv - Biochemistry 2022Quote: ... bacteria were cultured and plasmids were harvested according to the QIAGEN Plasmid Plus Maxi Kit protocol (QIAGEN). For mammalian expression and purification of KWOCAs and other secreted proteins ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was extracted directly from the pooled colonies using a QIAGEN Plasmid Maxi Kit (QIAGEN, #12162).
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bioRxiv - Genomics 2023Quote: ... the epegRNA lentiviral plasmid libraries were extracted using a Qiagen Plasmid Midi Kit (Qiagen, Cat. No. 12143). Extracted plasmid DNA from the four libraries was pooled to generate a pool with 3,825 epegRNAs and used for lentivirus generation.
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bioRxiv - Developmental Biology 2023Quote: ... Colonies were scraped and plasmid DNA (pDNA) of the sgRNA library was prepared (HiSpeed Plasmid Maxi, Qiagen). Deep-sequencing confirmed that all sgRNAs were well represented in the EpiChromo library ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria were cultured in lysogeny broth overnight and plasmid was harvested with Plasmid Plus Midi Kit (QIAGEN). This intermediate library and pJR98 were digested using BsmBI and the insert from pJR98 was ligated into the intermediate library to insert constant region CR3* for the first sgRNA and the hU6 promoter for the second sgRNA ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were amplified in competent E.coli and purified by using an Endofree plasmid max kit (#12362, Qiagen). Plasmids DNA were sent for Sanger sequencing to confirm the introduced SIRT5 variants (BaseClear ...
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bioRxiv - Neuroscience 2023Quote: ... All the plasmids for AAV vector preparations were made using endotoxin free plasmid maxiprep kit (#12663, Qiagen). PEI mediated co-transfection of pAAV plasmid-containing the transgene ...
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bioRxiv - Genomics 2023Quote: ... The transfected plasmid DNA was extracted from the cells using QIAGEN Plasmid Plus Midi kit (QIAGEN 12943). The sequencing ready plasmid library was generated by PCR with the extracted plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids from selected colonies were sequenced before scaling up their purification with EndoFree Plasmid Maxi kit (Qiagen) and subsequent transfection.
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bioRxiv - Biophysics 2021Quote: ... coli culture were harvested next morning to extract the plasmid library using the QIAfilter Plasmid Midi Kit (QIAGEN).
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transformed into XL1-Blue competent cells and plasmid DNA extracted using QIAprep Spin Miniprep Kit (Qiagen). The BUB1 K821R and BUB1C D946N alleles were generated by single-base substitution using site-directed mutagenesis (Strategene ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids and PCR products were purified with QIAprep plasmid Miniprep kit and QiaQuick PCR purification kit respectively (Qiagen). Plasmid Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were isolated from cell pellets using the EndoFree Plasmid Maxi Kit (0.45 g cells/column; Qiagen, 12362). Sequencing-based barcode quantification (see below Quantifying barcodes from plasmids and rabies virus genomes ...
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bioRxiv - Microbiology 2019Quote: ... Coli Top10 strain was transformed with plasmids followed by their amplification and isolation using Plasmid Midi Kit (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... plasmids were isolated from 200 mL of these cultures using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965). The remaining 200 mL of the overnight cultures were spun down ...
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bioRxiv - Cancer Biology 2022Quote: ... Colonies were harvested using a cell scraper and plasmids were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN). We confirmed homogeneous library representation by next-generation sequencing (Figure S2C).
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bioRxiv - Developmental Biology 2022Quote: ... All plasmids used for in ovo electroporation were purified using the 16 Qiagen Plasmid Plus Midi Kit (Qiagen). The total concentration of DNA ranged for each set of experiments between 0.5–1μg/μl ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were purified from positive clones with a QIAprep Spin Miniprep plasmid kit as instructed (Qiagen; Germantown, MD), and verified by Sanger automated sequencing (ACGT ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA from the Dub-seq library samples was extracted either individually using the Plasmid miniprep kit (Qiagen) or in 96-well format with a QIAprep 96 Turbo miniprep kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: The recombinant plasmid DNA derived from positive clones was isolated by using the QIAGEN plasmid mini kit (QIAGEN) and digested by PmlI (vector PFLD ...
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bioRxiv - Genomics 2022Quote: ... Plasmids were prepared using a Qiagen Plasmid Plus Midi Kit and a QIAvac vacuum manifold (Qiagen, Cat#: 12145).
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bioRxiv - Genomics 2021Quote: ... The bacterial cells were harvested by centrifugation and plasmid DNA was isolated using EndoFree Plasmid Giga kit (Qiagen).
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bioRxiv - Genomics 2021Quote: ... The bacterial cells were harvested by centrifugation and plasmid DNA was isolated using EndoFree Plasmid Giga kit (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... Each plasmid was first propagated in DH5α cells and later isolated utilizing the plasmid isolation kit from Qiagen. SH-SY5Y cells were transiently overexpressed with respective plasmids via standard protocol provided for Lipofectamine3000 mediated transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were grown using ByLss cell transformation procedures and extracted using the EndoFree Plasmid Maxi Kit (Qiagen, 12362). Final nucleic acid concentrations were determined using a NanoDrop (Thermo ...