Labshake search
Citations for Qiagen :
3851 - 3900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Purity and absence of unmodified viruses were assayed by PCR and Sanger sequencing after DNA extraction (DNeasy kit, Qiagen, Germantown, MD, USA). Viral stocks were produced as specified above and titered by plaque assay.
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bioRxiv - Microbiology 2024Quote: ... using QuantiTect multiplex PCR mix (Qiagen, Germantown, MD, USA), using the following PCR cycling conditions ...
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bioRxiv - Microbiology 2024Quote: ... and eluted into 100 µl AE buffer (Qiagen, Germantown, MD, USA). 10 µl of eluted DNA was used to setup 30 µl real-time Taqman quantitative PCR reactions ...
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bioRxiv - Microbiology 2024Quote: ... total DNA was isolated using either DNeasy Blood & Tissue Kits (Qiagen Inc., VIC, Australia), or DirectPCR™ Lysis Reagent (Direct buffer ...
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bioRxiv - Microbiology 2024Quote: ... Total genomic DNA was extracted with a DNeasy PowerBiofilm Kit (QIAGEN) for a total of 75 samples (Table S1) ...
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bioRxiv - Microbiology 2024Quote: We extracted and purified genomic DNA from tissue samples using a DNeasy Plant Pro Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The DNA for short read sequencing was extracted using the DNeasy Blood and Tissue Kit (QIAGEN), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Real-time qPCR was performed to quantify relative bacterial gene abundance with RT2 SYBR Green Fluor qPCR Mastermix chemistry (Qiagen, Limburg, Netherlands) and the CFX Connect thermocycler (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) was performed under the following conditions ...
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bioRxiv - Genomics 2024Quote: ... Spleen and liver were extracted using QIAamp DNA Micro Kit (Qiagen) suitable for starting material of less than 5 mg ...
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bioRxiv - Genomics 2024Quote: ... The amplicon library pools were isolated based on size by gel electrophoresis using a 2% agarose gel and then purified using QIAEX II Gel Extraction Kit (QIAGEN) and using 30uL of QIAEX II beads for each sample ...
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bioRxiv - Genomics 2024Quote: ... floridanum males using the Blood & Cell Culture DNA Maxi Kit (Qiagen Co. Ltd., Valencia, CA, USA). The genetic system of C ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was loaded onto a 1 ml StrepTactin column (Qiagen) at a flow rate of 0.7 ml/min in the ÄKTA prime-plus liquid chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from 200 µl of swab digestion buffer using QiaAmp 96 DNA Blood Kits (Qiagen, Germantown, MD, USA) and eluted into 100 µl AE buffer (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... gel purification (Qiagen) from a 0.8% agarose gel was carried out ...
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bioRxiv - Genomics 2024Quote: ... coli culture were harvested next morning to extract the plasmid library using the QIAfilter Plasmid Midi Kit (QIAGEN). Finally ...
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bioRxiv - Genomics 2024Quote: ... Tissues were finally homogenised with the TissueRuptor II device (Qiagen) making use of its maximal settings ...
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bioRxiv - Genomics 2024Quote: ... reaction to remove single-stranded DNA and further by column purification (MiniElute Gel Extraction Kit, QIAGEN).
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bioRxiv - Genomics 2024Quote: ... Next the correct sizes of the linearized backbones were isolated using gel electrophoresis and later purified using QIAquick Gel Extraction Kit (QIAGEN). For the library of 7 domains ...
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bioRxiv - Microbiology 2024Quote: RNA was isolated from the samples using the RNeasy Mini kit (Qiagen) and prepared for sequencing as described (17 ...
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bioRxiv - Microbiology 2024Quote: ... Equal measures of all samples were thereafter pooled and purified via PCR column clean-up (Qiagen). About 17.5 pmol of each pooled sample was reconditioned in an additional 50µl reaction with primers P1 and P2 ...
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bioRxiv - Microbiology 2024Quote: ... About 1 A600 unit of culture was harvested and RNA was extracted using RNeasy (Qiagen). cDNA was synthesized after ezDNase treatment using the SuperScript VILO cDNA kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... jejuni isolates using the EZ1 Advanced XL (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... All analyses in CLC-Genomics Workbench (Qiagen, Germany) and the Linux environment were conducted on a local server hosted by the Plant Systems Genetics lab at Seoul National University ...
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bioRxiv - Plant Biology 2024Quote: ... mRNA was on-column DNase treated with DNAse Set (Qiagen). Library preparation was performed by Novogene ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then homogenized using Tissuelyser II (Qiagen, Germany). Then 500 µL of chloroform was added and centrifuged at 15,000 rpm for 10 minutes to collect supernatant ...
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bioRxiv - Physiology 2024Quote: Total RNA was extracted from cultured HConjECs using the RNeasy Micro Kit (Qiagen), and mRNA expression was analyzed via reverse transcription polymerase chain reaction (RT-PCR) ...
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bioRxiv - Physiology 2024Quote: ... Pathway analyses were performed using Gene Set Enrichment Analysis (GSEA) v4.1.0 and Ingenuity Pathway Analysis (IPA) (QIAGEN Inc. ...
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bioRxiv - Physiology 2024Quote: Extraction and purification of RNA from the RV were performed with RNeasy Mini Kit (QIAGEN 74104) and detailed in Supplementary data.
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bioRxiv - Physiology 2024Quote: RNA was isolated from hearts using the RNeasy Fibrous Tissue Mini Kit (Qiagen, Germantown MD, USA). RNA concentration was determined by NanoDrop Spectrophotometer (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA extraction from cell pellet was performed by using RNeasy kit (Qiagen, 74104) following manufacturers protocol ...
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bioRxiv - Systems Biology 2024Quote: ... Genomic DNA was eluted with 110 μL buffer AE (Qiagen) preheated to 56°C and then stored at −20°C.
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted using RNeasy Plant Mini kit (Qiagen), and cDNA synthesis and quantitative PCR (qPCR ...
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Tuning the performance of a TphR-based terephthalate biosensor with a design of experiments approachbioRxiv - Synthetic Biology 2024Quote: ... A total of ∼7×104 individual colonies were then pooled and the plasmid library DNA was isolated by midi-prep kit (Qiagen, UK). 10 μg of the resulting library DNA was then transformed into P ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR-amplified templates were purified (QIAquick PCR purification kit, Qiagen #28106) and verified for the presence of a single DNA band of the expected size on a 1% TAE-agarose gel ...
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bioRxiv - Immunology 2024Quote: ... as per the manufacturer’s protocol (Qiagen). cDNA were done using the High-quality RNA to cDNA kit (Thermo Fischer ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted using the RNeasy® Plant Mini Kit (Qiagen) as described(Redkar et al. ...
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bioRxiv - Genetics 2024Quote: ... Pyrosequencing analysis was performed using PyroMark Q24 (QIAGEN GmbH, Hilden, Germany) to evaluate quantitative allelic expression ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA from various embryonic stages was extracted using TRIzol and cleaned up with the RNeasy Micro Kit (Qiagen 74004). cDNA synthesis was performed using the SuperScript III First-Strand Synthesis System (Thermo Fisher Scientific 18080051) ...
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bioRxiv - Biophysics 2024Quote: ... The cleared lysate was then loaded onto Ni-NTA (QIAGEN) equilibrated in 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... on a Rotor-Gene Q (Qiagen). Primers were added and PCR reaction conditions were chosen according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Hippocampal genomic DNA was isolated from adult female cross-foster (CF) and control (non-CF) offspring samples using the DNAeasy kit (Qiagen, Valencia, CA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... or QIASymphony DSP virus/pathogen Midi kit (Qiagen, cat. 937055) on a QIASymphony SP laboratory automation instrument platform according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using the RNeasy Plus Mini Kit (Qiagen, catalog number: 74134), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The QiaAmp viral Mini kit (Qiagen) or the PureLink Viral RNA/DNA Mini kit (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... PCRs included 0.5 units of HotStarTaq (Qiagen; Hilden, Germany; cat# 203205), 0.2µM primers ...
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bioRxiv - Immunology 2024Quote: ... The RNAse-Free DNAse set was used to digest DNA during RNA purification according to the manufacturer’s instructions (Qiagen). The purity of RNA was assessed by calculating the ratio of OD260/OD280 ...
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bioRxiv - Genetics 2024Quote: ... purified using MiniElute PCR Purification kit (Qiagen) and subjected to deep 2 x 150 bp paired-end next generation sequencing (NGS ...
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bioRxiv - Immunology 2024Quote: RNA was extracted using the RNeasy mini kit (Qiagen) as instructed by the manufacturer ...
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bioRxiv - Genetics 2024Quote: ... RNA was isolated using QIAzol reagent (QIAGEN Science, USA) according to the manufacturer’s protocol ...