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Citations for Qiagen :
3651 - 3700 of 3706 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Genomic DNA was then isolated from 1-5 million isolated PBMCs using the DNeasy Blood and Tissue kit (Qiagen; Cat. No. 69504) following manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNAs containing HIV-1 proviruses were recovered by infecting viruses produced in derivatives of THP-1 or SupT11 cells into SupT11 using DNeasy Blood & Tissue Kits (Qiagen, Cat# 69504). Following DpnI digestion ...
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bioRxiv - Molecular Biology 2023Quote: Total RNAs were prepared from livers of rats subjected to inhalational anesthesia with sevoflurane or desflurane for 6 hours or less than 1 minute using an RNeasy Midi Kit (QIAGEN, Venlo, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Each saliva sample was vortexed and 1) 500-750 μl was pipetted into a Powersoil Bead Tube (MOBIO PowerSoil DNA Isolation Kit, QIAGEN Cat #12888) for DNA extraction within two hours of collection (fresh) ...
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bioRxiv - Genetics 2023Quote: ... We isolated the entire exon 1 of HTT with 115 CAG repeats from an HD patient with UltraRun® LongRange PCR Kit (QIAGEN, 206442) with supplementation of 10% DMSO under the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... were weighed and homogenized in 1-10 µL/mg DMEM with a sterile glass bead at 30 Hz for 4 minutes using a TissueLyser (Qiagen, Germantown, MD) automated homogenizer ...
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bioRxiv - Cancer Biology 2023Quote: ... After incubations cells were pickup in buffer RLT with 1% of β-mercaptoethanol and total RNA purification proceeded following the instructions of RNeasy Plus mini kit (Qiagen, USA). Quality control of total RNA was carried out by spectrophotometric readings of optic density (OD ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA sequencing on the pancreatic xenografts in the orthotopic mouse model using PDAC cells (AsPC-1 ± SERPINB3) was also performed following isolation of RNA with the RNeasy Plus Mini Kit (QIAGEN, Venlo, Netherlands). Sequencing was performed on NovaSeq 6000 SP (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated following the Powersoil DNeasy kit protocol with the addition of a 10 min bead beating step at 50 s-1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 45 cycles of denaturation at 95 °C for 15 s and annealing at 60 °C for 1 min following a melting step in a Rotor-Gene Q machine (Qiagen, Hilden, Germany). GAPDH was used for normalization ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted from Tak-1 thallus according to the method described in (Tsuzuki et al., 2019) or using the DNeasy Plant Mini kit (QIAGEN, Hilden, Germany).
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bioRxiv - Microbiology 2023Quote: ... Tissues were thawed at the time of titration and homogenized for one minute at a frequency of 26 sec-1 in a TissueLyser (Qiagen, Haan, Germany) prior centrifugation for 5 minutes at 16,100xg to pellet debris ...
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bioRxiv - Systems Biology 2024Quote: ... were transfected with one of three different commercial siRNA sequences designed to target the EGFR or an Allstars negative control siRNA sequence (final concentration 1 nM; all Qiagen Ltd., UK) using INTERFERin transfection reagent according to the manufacturer’s instructions (Polyplus Transfection ...
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bioRxiv - Genomics 2024Quote: ... Total RNA was extracted from approximately 1 g of fresh gametophytic tissue using the RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Biochemistry 2024Quote: ... Hippocampal lysates were prepared by the addition of ice-cold PBS (200 µL PBS/10 mg hippocampal wet weight) and homogenized for 1 min in a TissueLyser II instrument (Qiagen, Hilden, Germany). One fourth of the obtained volume of each lysate was used for the analysis of total PLP concentrations as described below ...
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bioRxiv - Molecular Biology 2024Quote: ... Products were resolved on a 1% (w/v) agarose gel and gel purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Eye tissue was suspended in Buffer RLT/eye (1 eye/mouse; 10-18 mice/genotype) and prepared using the RNeasy kit (Qiagen, Germantown, MD). RNA quality was assessed by the 18S/28S rRNA bands in capillary electrophoresis (Bioanalyzer ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products was resolved in 1% agarose gel and the target band was gel purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). The PCR products were subsequently subjected to direct Sanger sequencing using G680V-F to for the adjacent G680V – A684G SNPs and V528L Seq2 for the V528L SNP ...
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bioRxiv - Genetics 2024Quote: ... Frozen tissue samples were homogenised by adding 600 uL RLT containing 1% β-Mercaptoethanol and a 5mm stainless steel bead (Qiagen # 69989) and processing for 40 seconds at 15 Hz with the TissueLyser II ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of the template was used in a 25 μL reaction using the QuantiFast Probe RT-PCR Kit (Qiagen, Inc., Valencia, CA) with 0.4 μM of each primer and 0.2 μM probe ...
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bioRxiv - Molecular Biology 2019Quote: ... Residual inactivation beads were removed by spinning the RNA sample through a QIAshredder column at 1000 g for 1 min (Qiagen-catalogue no. 79654). 2μg of total RNA input was retained for each sample and 30μg was incubated for 1.5 h at RT with 100 μg of Biotin-HPDP (Pierce - catalogue no ...
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bioRxiv - Genetics 2021Quote: Gene Ontology (GO) enrichment and network analysis of RNAseq results were performed using both Ingenuity Pathway Analysis (IPA) software package (Qiagen version 1-16) and manually developed excel pipeline workflows ...
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bioRxiv - Developmental Biology 2021Quote: ... Lower jaws were resuspended in 600 μl of RTL plus buffer supplemented with 1% β-mercaptoethanol (M3148-100ML, MilliporeSigma, Burlington, MA, USA) and Reagent DX (19088, Qiagen, Hilden, Germany). HH31 and HH34 lower jaws were processed in a Bead Mill 24 Homogenizer (15-340-163 ...
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bioRxiv - Developmental Biology 2020Quote: ... Lower jaws were resuspended in 600 μl of RTL plus buffer supplemented with 1% β-mercaptoethanol (M3148-100ML, MilliporeSigma, Burlington, MA, USA) and Reagent DX (19088, Qiagen, Hilden, Germany). HH31 and HH34 lower jaws were processed in a Bead Mill 24 Homogenizer (15-340-163 ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were checked on 1% agarose gels before being purified using a QiaQuick gel extraction kit (Qiagen, Inc., Valencia, California, USA) and directly sequenced in both directions using the amplification primers on an ABI 3730 automated sequencer (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from the supernatant of Vero E6 cells after 1 passage using the QIAamp Viral RNA Mini kit (Qiagen, https://www.qiagen.com/us/) without addition of carrier RNA ...
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bioRxiv - Genetics 2019Quote: ... DNA was isolated from 1 g of unexpanded leaves with CTAB method and purified with Genomic Tip 20/G (Qiagen K. K. Tokyo). The extracted DNA was sheared into 20 kb fragments using g-TUBE (Covaris ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then stained by Coomassie-blue or immunodetected as described before [26] using primary polyclonal antibodies directed against His6 epitope-tag (Penta His, Qiagen, dilution 1:1000), V5 epitope-tag (Bethyl Laboratories ...
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bioRxiv - Genetics 2022Quote: ... The tissue powder was then transferred to a 1.5 mL centrifuge tube where 400 μL of 1% PVP-40 Buffer AP1 solution and 4 μL of RNase A were added (Qiagen DNeasy Plant Kit Qiagen Inc, Hilden, Germany). The tube was initially vortexed to homogenize the solution before incubation at 65 °C for 30 minutes with a short vortex every 5 minutes.
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bioRxiv - Microbiology 2021Quote: ... approximately 1×106 Jurkat cells were washed twice in PBS and total DNA was extracted with the Qiamp® DNA Minikit (Qiagen, Manchester, UK). Quantitative PCR reactions were carried out as previously described using 0.3pmol each primer and 0.15 pmol of the probe in 25 μL volume containing 100-300ng total DNA using an ABI Prism® 7000 Sequence Detection System (SDS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 1 μg of total RNA was used for cDNA conversion using the RT2 first strand kit (Cat# 330404, Qiagen, Valencia, CA, USA). The prepared cDNA was diluted and used to determine the expression of genes of interest using specific primers ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 female and/or 1 male per litter) were homogenised and total RNA was extracted using RNeasy Mini Kits (liver; Qiagen, Chadstone, VIC, Australia) or Trizol reagent (muscle and adipose tissue ...
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bioRxiv - Genetics 2022Quote: Genomic DNA (gDNA) was extracted from 1-month-old Arabidopsis thaliana rosette leaves with the DNeasy plant mini kit (Qiagen, cat. no. 69104) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and 1:10 dilution of cDNA was used to quantify genes using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, USA). ADP-Ribosylation Factor 1 (AB050957.1 ...
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bioRxiv - Pathology 2024Quote: A sample of ca 1 mm3 excised from the middle section of left lateral liver lobe was cryo-homogenized using TissueLyser II (QIAGEN, Germantown, MD, USA), resuspended ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time quantitative PCR was performed on 40 pg cDNA using specific primers (Supplementary Table 1) and miRCURY® LNA® miRNA SYBR® Green PCR (Qiagen). Values were normalized to miRNA quantity and expressed as relative expression to control WS using formulae 2−ΔCT.
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were washed once with 1×PBS after which DNA was isolated using the QIAamp DNA Mini Kit (Qiagen, Venlo, The Netherlands) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from GFP-empty vector-transfected and GFP-Δ(1-240) JPh1-transfected C2C12 myoblasts using the RNeasy plus mini kit (Qiagen, Hilden, Germany), based on manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5μl of diluted cDNA (1:3 - nuclease-free water) was used as template in 10μl real-time PCR reactions (Qiagen: ‘SYBR Green PCR kit’) to assay specific transcripts (BioRad ...
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bioRxiv - Physiology 2023Quote: ... Ganglia in 1 mL of TRIzol reagent were homogenized using a TissueLyzer II bead mill (one 5 mm stainless steel bead per tube, 30 s-1 frequency for 3 minutes; Qiagen Inc. Hilden, Germany). The homogenates were incubated with 200 µL chloroform and centrifuged for 15 minutes at 12000 x g to isolate the protein-containing organic phase ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Neuroscience 2023Quote: ... The DNA was separated on a 1% agarose gel and the appropriate band was excised and isolated using a gel extraction kit (Qiagen, Valencia, CA, USA). In-Fusion HD enzyme premix was added to the linearized PCR product and transformed into the Stellar™ Competent Cells (Takara ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We verified the PCR products by 1% agarose electrophoresis with EtBr staining and purified using the QiaQuick PCR Purification Kit (Qiagen, Hilden, Germany, 28104) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... These leftover materials were collected by LCM and placed into 350 μl of RLT lysis buffer (RNeasy, Qiagen, including 1%β-Mercaptoethanol (β-Me)) followed by RNA extraction and examination with the 2100 Bioanalyzer.
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bioRxiv - Plant Biology 2020Quote: ... Frozen cells were disrupted by bead beating at 30 h/z for 1 min before purifying total RNA using an RNeasy® plant mini kit (Qiagen™; ref. 74904) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Samples were subjected to dry pulverization before being homogenized in buffer (350 μL of Qiagen RLT buffer with 1% β-mercaptoethanol; Qiagen, Germantown, MD, USA). The homogenate was transferred to an RNase-free 1.5 mL centrifuge tube ...
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bioRxiv - Microbiology 2019Quote: DENV viral RNA extraction was performed from acute serum samples (Baseline, 1-10, and 15 dpi) using QIAamp Viral RNA mini kit (Qiagen Inc, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... we ran the digest products on 1% Tris-acetate EDTA gels and extracted and purified the correctly sized products (Qiagen QIAQuick Gel Extraction Kit). In parallel ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from MLEC and lung tissue by lysis in RLT buffer containing 1% β-mercaptoethanol for RNA isolation and subsequently purified using the RNeasy Mini Kit (Qiagen, 74106, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantification of the relative miRNAs’ expression level was detected by specific miRCURY LNA miRNA PCR Assays and highly sensitive miRCURY LNA SYBR® Green PCR Kit while adjusting the reverse transcriptase product dilution to 1:30 (QIAGEN, Cat. No. 339345). The calculation of relative miRNA expression was based on the -ΔΔCT method ...