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Citations for Qiagen :
251 - 300 of 5837 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues dissected from SD and control mice were homogenized in QIAzol Lysis Reagent (Qiagen, Hombrechtikon, Switzerland). RNA was extracted and purified using the QIAGEN miRNeasy mini kit 50 (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA of equal pools of (n = 15) larval brains was extracted using the RNeasy kit (Qiagen). RNA was reverse transcribed to cDNA using the Super Script III First-strand synthesis system (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Purification of total RNA from fatty tissues as brain was performed using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA from brain regions was extracted using an automated nucleic acid extraction platform called QIAcube HT (Qiagen) with a column-based extraction kit ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 25 mg brain samples using the DNeasy PowerSoil Pro Kits (Qiagen, Germany, 47014) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from brain lysates and purified myelin-enriched fractions using the miRNeasy Mini Kit (Qiagen) and the concentration was increased using Co-precipitant pink (Bioline) ...
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bioRxiv - Neuroscience 2024Quote: ... and mRNA from brain microvessels was isolated using the QIAamp Lipid Tissue Mini Kit (Qiagen, Cat# 74804). Total RNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: RNA from human microglia differentiated in vitro and human microglia extracted from human-mouse chimeras was extracted using the RNeasy Plus Micro Kit (Qiagen). The RNA from 3 independent in vitro microglia differentiations per cell line were pooled at equal weight to obtain six samples ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: The human Hh signaling target PCR array (Qiagen) profiled the expression of 84 key genes responsive to Hh signal transduction ...
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bioRxiv - Microbiology 2021Quote: Human colon resections were placed in RNAlater (Qiagen) at the time of resection ...
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bioRxiv - Physiology 2024Quote: ... and Qiagen human Primer Assays (Qiagen, Hilden, Germany) specific for FoxO1 and FoxO3 (the latter only in NHBEs) ...
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bioRxiv - Microbiology 2023Quote: The human Hippo signaling target PCR array (Qiagen) was used to determine expression of 84 key Hippo target genes ...
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bioRxiv - Microbiology 2023Quote: Human Inflammatory Response & Autoimmunity Focus) (Qiagen, YAHS-205Z) were used for each of the different samples ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from cultured from human bronchial epithelial cells / mice right lung tissues and purified using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany), supplemented with the Proteinase K (Qiagen ...
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bioRxiv - Genetics 2019Quote: Total RNA from human cell lines (PTC-05, HepG2 and HEK-293T) was extracted using spin columns with the RNeasy Mini Kit (QIAGEN, GmbH, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of the BCL-2 anti-apoptotic genes in the NPC cell lines were accessed using the custom RT2 Human Apoptosis Profiler PCR array (Qiagen, Hilden, Germany). To delineate the contribution of MCL-1 for cell survival ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA from Amborella generative cells and sperm cells was extracted according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN, Hilden, Germany). In brief ...
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bioRxiv - Microbiology 2023Quote: ... 293A cells were transfected with the plasmid of pCDNA3.1-ACE2 (human)-3×FLAG (MiaoLing Plasmid Platform, China) using polyfect (QIAGEN, West Sussex, UK) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS sorted mouse brain cells stabilized in RNAprotect buffer according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN, Hilden, Germany). In brief ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng of cDNA was used for qPCR with the Human Cellular Senescence and Human Aging RT2 Profiler™ PCR Arrays (Qiagen). Arrays were run on the 7300 Real Time PCR System (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA from the control human TSCs as well as WWTR1-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, 74104) per the manufacturer’s protocol with on column DNase digestion ...
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bioRxiv - Molecular Biology 2021Quote: Hepatic gene expression was investigated using qPCR arrays designed for human (RT2 Profiler PCR arrays – ‘Human Fatty Liver’, Qiagen France SAS). Then ...
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bioRxiv - Neuroscience 2020Quote: ... RNA from total spinal cord and brain lysates as well as corpus callosum was isolated using QIAzol (Qiagen) and the RNeasy protocols (Qiagen) ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from dissected cortices of E14.5 embryonic brains by using an RNeasy Micro Kit (Qiagen). A sequencing library was prepared using a TruSeq Stranded Total RNA Kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: Isolation of RNA from homogenized lung and brain tissue was performed with a QIAamp Viral RNA Kit (QIAGEN) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was then extracted from whole zebrafish brains using the QIAGEN RNeasy⍰ Mini Kit (Qiagen, Venlo, Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: We isolated RNA from the right hemisphere brain regions described above using the RNeasy 96 kit (Qiagen, 74181) and a TissueLyser II (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNAs were isolated from the brain and liver samples using a miRNeasy Mini Kit (Qiagen, Hilden, Germany). cDNAs were synthesized from total RNAs using iScript reverse transcription supermix for RT-qPCR (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... lungs and gut and P7 forebrain and mid brain samples by RNAeasy mini kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... brain sections and EVs following the protocol recommendations of the RNeasy Plus Micro Kit (cat. no. 74034, Qiagen) and AllPrep DNA/RNA/Protein Mini Kit (cat ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated from a KI mouse brain hemisphere using the RNeasy Plus Universal Mini Kit (Qiagen, 73404), 2 µg of RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit with random hexamer primers (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Each brain was dissected on dry ice into roughly ten pieces which were placed in QIAzol (Qiagen, 217004) and immediately homogenized using a pellet pestle ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from 1mm diameter tissue punches of approximately 20 μg of frozen brain tissue (Qiagen RNeasy), assessed for suitable mass (RiboGreen) ...
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bioRxiv - Neuroscience 2024Quote: ... whole brains were harvested for RNA extraction using the RNeasy Lipid Tissue Mini Kit (74804, Qiagen, Hilden, Germany). RNA concentration and purity were determined by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA from the control human TSCs as well as GATA2-KD and GATA3-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, Germantown, MD) per the manufacturer’s protocol with on-column deoxyribonuclease digestion ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR array for human cell cycle (Cat#PAHS-020Z) and oncogenes and tumor suppressor genes (Cat#PAHS-502Z) was purchased from Qiagen (Valencia, CA, USA), and was used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... or the RNeasy micro kit (Qiagen, cat# 74004; primary neurons).
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bioRxiv - Cell Biology 2021Quote: Oligonucleotides for siRNA included: human βPix (synthesized by Qiagen), human myosin-18A (synthesized by Qiagen)
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bioRxiv - Immunology 2024Quote: ... RT2 Profiler PCR Array Human Inflammatory Response & Autoimmunity (Qiagen) was used according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: Frozen tissues (0.5-1cm3) from the following brain regions from each case were macrodissected into RNAlater RNA Stabilization Reagent (Qiagen): 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from a bank of frozen brains kept at −80 °C using a RNA isolation kit according to the manufacturer’s instructions (Qiagen). Frozen brain samples were thawed and dissected from 5 different regions ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from fresh-frozen brain tissue (Brodmann area 9) using the RNeasy lipid tissue mini kit (QIAGEN); 20 mg of tissue was homogenised in 1 mL QIAzol lysis reagent using a 1 mL syringe successively with 23G and 25G needles ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from snap frozen brain areas (both hemispheres for hippocampus) using the RNeasy Plus Universal Kit (QIAGEN), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... fly brains were dissected in 1X PBS and genomic DNA was isolated using the QIAamp DNA Micro kit (Qiagen). Genomic DNA was then digested with DpnI and purified using spin column purification (Qiagen PCR-purification kit) ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from right hemisphere brain lysates using a QIAGEN RNeasy kit (Catalogue #74104, QIAGEN, Hilden, Germany) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from FACS-purified astrocytes from a single mouse brain using the QIAshredder and the All-prep DNA/RNA/protein Mini Kit according to the manufacturer’s protocol (Qiagen). Total RNA concentration was measured using a NanoVue PlusTM spectrophotometer (Biochrom) ...
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bioRxiv - Neuroscience 2023Quote: The genomic DNA was isolated from mice brain cortex tissue using the DNeasy blood and tissue kit (Qiagen 69504) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... We extracted total RNA from most adult tissues (minus the brain) using the RNeasy Kit (QIAGEN, Redwood City, CA) and submitted the extracted total RNA to Novogene Ltd ...