Labshake search
Citations for Qiagen :
251 - 300 of 326 citations for Mycoplasma Agar 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: BMDM treated in 6-well plates were washed twice with PBS before total RNA purification using the RNeasy kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: Total RNA was purified from single wells of >85% confluent six-well plates using the RNeasy Plus mini kit (Qiagen), and an additional DNase I step was used to remove genomic DNA ...
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bioRxiv - Microbiology 2024Quote: DNA extraction from cultured treponemes propagated in 6-well plates following transformation and expansion was performed using the QIAamp DNA mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were collected from plates on the day noted and RNA was purified using the RNeasy mini prep kit (Qiagen), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... yeast plasmid DNA was extracted from 5x107 cells harvested off of galactose and final glucose plates of replicates 2 and 3 using Qiaprep Spin Miniprep Kit (QIAGEN). We amplified the 200-bp repair template region from the initial E ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA brain tissue punches were homogenized in 96-well plate format on a QIAGEN TissueLyser II (Qiagen, Valencia, CA; #85300) in 600 μL of homogenizing buffer (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The cells growing on the plate were scrapped and library phagemid DNA was prepared with a DNA Maxiprep kit (Qiagen).
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from three biological replicates of plate-grown seedling tissue (100 mg per replicate) using the RNeasy plant mini kit (Qiagen). Isolated RNA was treated with Ambion TURBO™ DNase (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... which was pre-wet with 1 mL cold buffer 1 before it was placed on the receiving plate attached to a QIAvac96 (Qiagen). Pressure was maintained below 100 bar during filtration ...
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bioRxiv - Microbiology 2024Quote: ... 6×105 cells were seeded onto type I collagen-coated 6-well plates and transfected with 200 ng of viral DNA using Attractene Transfection Reagent (Qiagen). Two days later ...
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bioRxiv - Plant Biology 2024Quote: A small piece of frozen leaf from each rice plant was placed in wells of 96-sample plates and dry-ground in the TissueLyser II (Qiagen) using steel balls ...
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bioRxiv - Microbiology 2024Quote: To determine bispecificity of bsAbs his-tagged HCV E2 (3 μg/mL in Tris-buffered saline (TBS)) was immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
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bioRxiv - Immunology 2024Quote: Reverse transcription and PCR I were performed in 384-well plates pre-loaded with 3 µL of Vapor-Lock (Qiagen). Cell lysis buffer and reverse transcriptase mix (0.4 µL/well ...
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bioRxiv - Cell Biology 2024Quote: For optimisation of pericyte growth conditions RNA was collected from pericytes seeded at 1×105 in a 12-well plate using the RNeasy Mini Kit (74104, Qiagen) after 24 h in the appropriate GA medium ...
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bioRxiv - Developmental Biology 2024Quote: ... single cells were transferred to individual wells of a 96-well plate containing 2.5 µl of RLT plus buffer (Qiagen, 1053393), frozen on dry ice and stored at −80°C ...
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bioRxiv - Molecular Biology 2021Quote: Cells were seeded in 6-well and 12-well plates at a density of 1.25×105-5×105 cells per well and transfected with miR-320a mimic (Qiagen, 339173 YM00471432) or antagomir (GenePharma ...
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bioRxiv - Immunology 2021Quote: ... Single cells were sorted into individual wells of a 96-well plate containing 5 μl of lysis buffer (TCL buffer (Qiagen #1031576) with 1% of 2-b-mercaptoethanol) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2.5×106 cells per well were seeded in six-well plates and transfected using Effectene transfection reagent (Qiagen, Cat. No. 301425). The transfection mixtures contained the following plasmids ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2.5×106 cells were seeded per well in 6-well plates and transfected using Effectene transfection reagent (Qiagen, Cat. No. 301425). The transfection mixtures in Figure 5A contained 1 μg ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from cells in a single well of a 6-well plate was isolated using the RNeasy Plus Mini Kit (Qiagen, 74134) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from cells (1-2 wells, 6 well plate) using an RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), including a DNase step to remove residual DNA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was isolated from iPSC-SNs seeded in 6-well plates using a RNeasy Mini Kit (Qiagen, Redwood City, CA) and reverse transcribed into cDNA using a SuperScript VILO cDNA Synthesis kit (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown to 80% confluence in a 6-well plate before RNA was extracted using standard RNA extraction protocol (RNAeasy Mini Kit, Qiagen, #74104). cDNA synthesis was performed by mixing 1μg of RNA with qScript® cDNA Synthesis Kit (Quantabio ...
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bioRxiv - Microbiology 2021Quote: DNA from plate-well pools of the progenitor collection was isolated with a DNeasy 96 Blood &Tissue Kit (Qiagen, Cat. #69582) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For mobility shift assay (Figure 4) ...
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bioRxiv - Cell Biology 2020Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol (with 10 µl Polyfect reagent per 35 mm plate).
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD14- CD16- CD20+CD38+ BG505 SOSIP+YU2-gp140+ B cells were sorted into individual wells of a 96-well plates containing 5 μl of a lysis buffer (Qiagen, 1031576) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Immunology 2020Quote: Initial crystallization hits were obtained via sparse matrix screening in 96-well plates using commercially available crystallographic solutions (Qiagen, Toronto, ON). Optimization of crystallization conditions was performed in 24-well plate format using the hanging drop vapor diffusion method ...
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bioRxiv - Biochemistry 2022Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol (with 10 µl Polyfect reagent per 35 mm plate).
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bioRxiv - Genomics 2022Quote: ... Individual nuclei were sorted on a Sony Sorter SH800 with a 100 μm sorting chip into wells of a 96-well plate containing 5 μl of Buffer TCL (Qiagen #1031576) with 1% β-mercaptoethanol (ThermoFisher #21985023).
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bioRxiv - Cancer Biology 2022Quote: ... Exosomal miRNAs were profiled using Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, #YAHS-106Y, Plate Format: 2 × 96-well).
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from cells grown in six-well plates at 70–80% confluency with an RNA extraction kit (Qiagen #74104). cDNA was synthesized using a sensiFAST cDNA synthesis kit (Meridian Bioscience #BIO-65053) ...
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bioRxiv - Bioengineering 2024Quote: ... These expression cultures were set up in 500 uL volumes in deep-well 96-well plates (square wells used for maximum culture aeration; USA Scientific 1896-2800) sealed with porous tape (Qiagen 19571) and incubated at 37°C and 350 RPM for 20 hr ...
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bioRxiv - Molecular Biology 2022Quote: Nunc MaxiSorp™ 96-well plates were coated with 75 ng/well of penta-His antibody in PBS solution (Qiagen, 34660) and incubated overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Total RNA was extracted from iPSC-SNs seeded in 12-well plates using RNeasy Mini Kit (74106, Qiagen AB, Stockholm, Sweden) with DNase digestion (79256 ...
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bioRxiv - Immunology 2022Quote: ... following the final boost (Week 18 or 23) were sorted into individual wells of a 96-well plate containing 5 μL of lysis buffer (TCL buffer (Qiagen, 1031576) with 1% of 2-β-mercaptoethanol) ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Colonies were transferred to 96-well plates and grown until a sufficient number of cells was reached for genomic DNA (DNeasy Blood & Tissue Kit; Qiagen, #69504) and protein extraction ...
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bioRxiv - Genomics 2023Quote: ... 50 nuclei were sorted into each well of 4 twin.tec™ 96 Well LoBind PCR Plates that had 5 uL of EB buffer (Qiagen, 19086), 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded at 3 × 105 cells.well−1 in 6-well plates and transfected 24 h later with one pmiRGLO derivative construct per well (0.9 µg plasmid) using Effectene (Qiagen cat. #301425). After 3 days the cells were washed with PBS and lysed with Cell Culture Lysis Buffer (Promega cat ...
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bioRxiv - Cancer Biology 2023Quote: ... medium was removed and cells were lysed on the plate by addition of 3 ml of lysis reagent RLT Plus (Lot 169010027, Qiagen, Germany). 1 ml of lysate was transferred to a 2 ml Eppendorf tube ...
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bioRxiv - Plant Biology 2023Quote: ... Sand and rhizosphere samples were prepared by adding 250 mg of the collected substrate to a PowerBead plate and homogenized using a TissueLyser II (Qiagen, USA) at a frequency of 20 Hz for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... MiRs (61 plus 3 controls) were quantified on a custom PCR plate using the miRCURY LNA miR SYBR Green PCR Handbook (Qiagen, Germany). PCR (40 cycles ...
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bioRxiv - Neuroscience 2024Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For coimmunoprecipitation (coIP ...
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bioRxiv - Microbiology 2024Quote: ... the plates were imaged to verify expression of the fluorescent reporter before column-based isolation of RNA (Qiagen, RNeasy Mini Kit) with on-column DNA digestion ...
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bioRxiv - Cell Biology 2024Quote: APOB expression determined by first isolating total RNA from Hep3B cells grown in 6-well plates using the RNeasy Mini Kit (Qiagen, #74104). Real-time qPCR was performed using the TaqManTM RNA-to-CtTM 1-Step Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Single viable tumor cells were selected by positive staining for Calcein AM as well as negative staining for TO-PRO3 and sorted into pre chilled (4 °C) 96 well plates containing TCL buffer (Qiagen, 1031576) + 1% β-mercaptoethanol (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... two coverslips from a 24-well plate of neurons were combined into 350 µL RLT buffer from the RNeasy Micro Plus kit (Qiagen, #74134), supplemented with 1% beta-mercaptoethanol (PanReac AppliChem ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The freshly isolated chondrocytes were plated at the density of 1.2 x 105/cm2 and reverse transfected on the PCR array plate (RT² Profiler™ PCR Array Mouse Extracellular Matrix & Adhesion Molecules, Qiagen). On the next day ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was later harvested from the plates by briefly thawing prior to using extraction by RNeasy Mini kit (Qiagen Cat# 74106) and QIAshredder (Qiagen Cat#79654) ...