Labshake search
Citations for Qiagen :
1551 - 1600 of 2888 citations for 7 Bromo 2H 1 3 benzodioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... 1 mL of the 2-mL culture was removed and replaced by 1 mL of RNAprotect Cell Reagent (Qiagen). After 5 min of incubation ...
-
bioRxiv - Microbiology 2022Quote: ... 200 RPM for 2 h then approximately 1×109 CFU of bacteria were mixed at a 2:1 (vol:vol) ratio of RNAProtect (Qiagen) and incubated at room temperature for 5 min ...
-
bioRxiv - Physiology 2020Quote: ... insulin-like growth factor 1 receptor (Igf1r) and sirtuin 1 (Sirt1) gene promoters were designed using the Pyromark Assay Deisgn 2.0 software (Qiagen). PCR and sequencing primers are provided in Supplementary Table 1 ...
-
bioRxiv - Plant Biology 2022Quote: RNA was extracted from 10-day-old whole seedlings grown on 1/2X MS media containing 1% sucrose and 0.8% agar (Plant RNeasy kit (Qiagen)) ...
-
bioRxiv - Microbiology 2023Quote: TG was quantified in snap-frozen liver tissue stored at −80 □C until cryo-grinding in liquid nitrogen and 50 ±5 mg tissue added 0.9 mL of a 2:1 chloroform:methanol solution and homogenized 1 min at 50 os/sec using a TissueLyser LT (Qiagen) with beads ...
-
bioRxiv - Systems Biology 2023Quote: ... x mg solid matrix were mixed with five times the μl amount of ACN:water (1:1, v/v) and homogenised with a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After a short centrifugation (2 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... or 1 μl 1:10 diluted oligonucleotides (for RNA from immunopurified ribosomes) from the QIAseq FastSelect –rRNA Yeast Kit (Qiagen) in 10.5 μl total at 75°C ...
-
bioRxiv - Immunology 2021Quote: ... DNA was extracted from circulating PD-1+ and PD-1− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing.
-
bioRxiv - Microbiology 2019Quote: ... A second round of amplification was performed by transferring 5µl of 1:100 diluted Round 1 product into 15µl Fast Cycling PCR Mix (QIAGEN, Hilden, Germany) reactions ...
-
bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from FACS purified Zeb1+ (Lineage−Sca-1+CD49fhi tdTomato+) or Zeb1− basal cells (Lineage−Sca-1+CD49fhitdTomato−) using a RNeasy micro kit (Qiagen). cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) ...
-
bioRxiv - Cell Biology 2019Quote: ... Lentivirus carrying the response elements for type I (ISRE - #CLS-008L-1) or type II (GAS - #CLS-009L-1) upstream of firefly luciferase was purchased from Qiagen. Twenty-four hours post plating ...
-
bioRxiv - Neuroscience 2020Quote: ... at 60°C overnight and 1 μl of a 1:20 dilution of the lysate used in a PCR reaction (HotStarTaq, Qiagen). The sequence flanking the tmt-opsin1b TALEN binding sites was amplified using the forward 5’-GGGACTTTCTTTGCGCTTTA-3’ and the reverse 5’-CAGGTCAGAGCGGATCTCAT-3’ primers ...
-
bioRxiv - Microbiology 2021Quote: ... qPCR reactions contained 4 μl of cDNA (1:50 dilution) and 200 nM of each primer (supplementary table 1) and QuantiTect SYBR Green PCR master mix (Qiagen) or SensiFAST SYBR Hi-ROX kit (Bioline) ...
-
bioRxiv - Bioengineering 2019Quote: ... the cells were washed once with 0.1% PBSA and incubated with 50 μL of a 1:100 dilution of anti-penta-His Alexa 647 antibody (Qiagen) for 10 minutes on ice ...
-
bioRxiv - Immunology 2019Quote: ... or M-MØ (1×106 cells/well) were transfected with 1 μg of the XRE reporter construct using either Superfect (Qiagen) or VIROMER RED (Lipocalix) ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with a 1:1 molar ratio of eGFP-P2A-Cas9 and single stranded oligonucleotide using Effectine (Qiagen) in a six-well dish ...
-
bioRxiv - Biochemistry 2020Quote: ... The restriction digestion efficiency was validated using 1% 1× TBE agarose gel electrophoresis and purified with a QIAquick PCR Purification Kit (Qiagen).
-
bioRxiv - Immunology 2021Quote: ... homogenized in 1 ml PBS by bead beating (3mm steel ball, 25 Hz for 1 minute in a TissueLyser (Qiagen)) ...
-
bioRxiv - Genomics 2021Quote: ... 1 g of ground leaf powder was resuspended in lysis buffer with 1 mg/ml of proteinase K (19133, Qiagen) and 190 μg/ml of RNase A (19101 ...
-
bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from 1×106 MCF7 and 1×106 BT-474 cells (‘bulk’) using the RNeasy Mini Kit (74104, Qiagen) in combination with the QIAshredder (79654 ...
-
bioRxiv - Immunology 2022Quote: ... For bacterial load determination one lobe of the lung was collected in 1 mL PBS with 1 metal bead and smashed using a tissue lyser (Qiagen). 25 μL of lysed lung were put in duplicates on CYE-plates and grown for 3-4 days at 37°C in a non-CO2 incubator.
-
bioRxiv - Physiology 2024Quote: ... After chloroform incubation and centrifugation per manufacture instructions RNA-containing phase was diluted with 70% ethanol 1:1 and RNA isolation was performed using RNeasy Mini Kit (Qiagen). For tissues ...
-
bioRxiv - Cell Biology 2023Quote: ... The aqueous phase was mixed 1:1 with 70% ethanol and applied to a minikit spin column and washed with RW1 and RPE buffer (Qiagen). cDNA was synthesized using the QuantiTect Rev ...
-
bioRxiv - Microbiology 2023Quote: ... A volume of 1:1 of 70% ethanol was mixed with the upper phase before transfer on column (RNeasy Mini kit, Qiagen), and RNA was extracted according to manufacturer directions ...
-
bioRxiv - Plant Biology 2023Quote: ... of g1090::XVE>>AXR3-1-mCherry and HS::AXR3-1 were extracted following the protocol (Plant Total RNA Mini Kit, QIAGEN). g1090::XVE>>AXR3-1-mCherry were transferred for 2 h on estradiol-containing medium and then harvested ...
-
bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
-
bioRxiv - Bioengineering 2022Quote: ... the aqueous phase was mixed with ethanol in a 1:1 ratio and was added to RNeasy spin columns (Qiagen) for RNA purification ...
-
bioRxiv - Cancer Biology 2023Quote: ... Digested PCR fragments were ligated into the restricted vector at a 1:1 molar ratio using T4 DNA ligase and were purified using the MiniElute PCR purification kit (Qiagen). Purified ligated plasmids were electroporated into MegaX DH10B (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... was added to the supernatant to give a final concentration of 1 mM and the resultant suspension was applied to a 1 ml Ni2+-NTA column (Qiagen). The resin was then washed sequentially with 10 ml of lysis buffer (without Triton X-100) ...
-
bioRxiv - Biophysics 2024Quote: ... The significantly differentially expressed genes with a log2 fold change <= −1 or >= 1 were input into Ingenuity Pathway Analysis (Qiagen) to determine pathway enrichment (significance determined by Fischer’s exact method).
-
bioRxiv - Molecular Biology 2024Quote: ... fungal DNA was extracted independently from 1 mL of crude conidial suspensions (between 108 and 109 spores mL-1) using the DNeasy 96 Plant Kit (Qiagen) following the manufacturer’s instructions (Gautier et al. ...
-
bioRxiv - Immunology 2021Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen) ...
-
bioRxiv - Genomics 2020Quote: ... and 0.5 mg.ml-1 of Proteinase K (Qiagen), to reverse formaldehyde crosslinking ...
-
bioRxiv - Microbiology 2019Quote: ... 1 ml RLT buffer (Qiagen RNA Isolation Kit) + 10 μl β-mercaptoethanol was added and vortexed and a phenol chloroform extraction followed by an ethanol precipitation was carried out ...
-
bioRxiv - Genomics 2020Quote: ... cells were mixed 1:2 with RNAProtect (Qiagen) and harvested via centrifugation ...
-
bioRxiv - Microbiology 2021Quote: ... and 1-5mL Qiazol Lysis Reagent (Qiagen, #79306) was added ...
-
bioRxiv - Cancer Biology 2020Quote: ... amplification grade (1 U/µg RNA, Qiagen, #79254). Real-time qPCR was performed with a MyiQ™ instrument (BIO-RAD) ...
-
bioRxiv - Biochemistry 2022Quote: ... Penta-His Antibody (Qiagen, 34660, IB: 1:1000) and Y188 to APP C-terminus (Abcam ...
-
bioRxiv - Molecular Biology 2019Quote: ... with on-column DNase-1 digestion (79254, Qiagen). Purified RNA had 260/280 ratios ~2 (via Nanodrop 2000c ...
-
bioRxiv - Neuroscience 2019Quote: ... 5nmol of Rho-1 siRNA (Qiagen, Germany #SI01401743) was diluted in rnase-free water (provided in kit ...
-
bioRxiv - Biochemistry 2021Quote: ... passed over 1 mL Ni-NTA resin (Qiagen), and bound protein eluted with 10 CV of buffer A / 200 mM imidazole ...
-
bioRxiv - Immunology 2021Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen):cDNA ...
-
bioRxiv - Genetics 2019Quote: ... 1× Type-it Multiplex PCR Master Mix (Qiagen), 0.2 μM of each locus specific reverse primers ...
-
bioRxiv - Genetics 2020Quote: ... 1× Multiplex PCR Master Mix (Qiagen, Hilden, Germany), and 0.2 μM of Primer_F3 and Primer_R (Figure S2 and Table S2) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen):cDNA) ...
-
bioRxiv - Genomics 2022Quote: ... 1 mL of buffer PB or PM (Qiagen), containing a high concentration of guanidine hydrochloride and isopropanol was added and mixed by pipetting ...
-
bioRxiv - Physiology 2022Quote: ... Samples were homogenized in 1 ml Qiazol (Qiagen) with 5 μl β-mercaptoethanol using a beadmill homogenizer cooled with liquid nitrogen and then phase separated using 1-bromo-3-chloropropane (BCP) ...
-
bioRxiv - Biochemistry 2024Quote: ... and anti-Strep (Qiagen, 34850, 1:1000 dilution) antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteinase K (20 μg·ml-1 final concentration, Qiagen) was firstly added to the relevant samples ...