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Citations for Qiagen :
1451 - 1498 of 1498 citations for Diazinon Unlabeled 1000 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 1 mL glycerol stocks were made for each library and a 200 mL LB-AMP outgrowth was grown to a concentration of 3 OD600/mL followed by a Qiagen MAXI plasmid prep (Qiagen Cat#12963).
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bioRxiv - Systems Biology 2023Quote: ... for genomic DNA extraction using 1.5 mL of cell culture with a phenol chloroform extraction and phase lock tubes (Qiagen Sciences, Germantown, MD) to enhance phase separation ...
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bioRxiv - Microbiology 2023Quote: Cultured cells were washed once in 10 ml PBS before RNA extraction using the RNeasy Plus Micro kit (Qiagen, Cat. No. 74034). RNA was eluted in 30 µl RNase free H2O and stored at -80◦C until use.
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bioRxiv - Microbiology 2023Quote: ... Single colonies were picked and grown overnight in LB broth with 50 μg/mL Kanamycin before plasmids were isolated using a QIAprep Spin Mini Kit (Qiagen, Germantown, MD), confirmed by restriction enzyme digest ...
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bioRxiv - Genomics 2023Quote: Genomic DNA for the Pacific Bioscience Single Molecule Real-Time (SMRT) sequencing was prepared from 2 mL of fresh blood using the genomic-tip 100/G kit (Qiagen, Hilden, Germany). This was performed with additional RNase (Astral Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was separately extracted from each 10 mL skinning sample using the DNeasy® mericon® Food Kit (Qiagen, Hilden, Germany). Each extraction was performed following the manufacturer’s instructions for standard large-scale extractions with minor modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... 600 µl of lysis buffer (TES (0.1 M TRIS, 10 mM EDTA, 2% sodium dodecyl sulphate; pH 8) and Proteinase K (Qiagen, 20 mg/ml) in a 20:1 ratio ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted directly from the frozen samples with the addition of 2 ml of G2 DNA/RNA Enhancer (Ampliqon, Odense, Denmark) using the RNeasy PowerSoil Total RNA Kit (Qiagen, København, Denmark) with phenol:chloroform:isoamyl alcohol following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... each Sterivex filter membrane was sectioned in half (representing approximately 500 ml of sample) and placed in Qiagen DNeasy Powersoil DNA isolation kit bead-beating tubes (Qiagen; Hilden, Germany) for immediate DNA extraction and the other half for storage at -80 °C ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 1 mL of each human plasma sample using the DNeasy Blood and Tissue kit (Qiagen, 69504, Hilden, Germany) according to the manufacturer protocol and recommendations ...
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bioRxiv - Microbiology 2023Quote: ... a 1 mL aliquot was sterile filtered and kept to test conditioned media for IL-10 induction (as above)) and replaced with 5 mL of RNA-protect (Qiagen, Cat. # 1018390), mixed by pipetting ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we homogenized 2-3 fecal pellets in 1 mL H2O using ceramic beads (NucleoSpin, Macherey–Nagel, Dueren, Germany) and a TissueLyser (Qiagen, Hilden, Germany), mixing the sample for 3 × 30 s at 4,500 rpm with a 10 s cooling break (< 0°C) ...
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bioRxiv - Cancer Biology 2023Quote: ... we adapted the same conditions used in Dip-C40 and lysed each nucleus in 150 nL of lysis buffer containing 20 mM Tris pH8/20 mM NaCl/25 mM DTT/0.15% Triton X-100/1 mM EDTA/5 µg/mL Qiagen Protease (Qiagen, cat. no. 19157). After dispensing ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred into the glovebox and loaded onto a gravity column in with 1 mL nickel-nitrilotriacetic acid resin (Qiagen, Hilden, Germany) that was preequilibrated with wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... The clarified lysate was then applied to a 10 mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...
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bioRxiv - Microbiology 2020Quote: ... Fresh frozen tissue homogenates were prepared by thawing frozen tissue and placing 200 mg (± 50 mg) of minced tissue in a tube containing 1 ml DMEM culture medium and a steel bead (Qiagen, Germantown, MD, USA). Homogenization was performed with the TissueLyser LT (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... about 20 mg of tissue was macerated with sterile blade and digested with 30 μl of Proteinase K (20mg/ml) and 300 μl of ATL buffer (Qiagen Inc., Hilden, Germany) overnight at 56°C ...
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bioRxiv - Genetics 2020Quote: ... we grew one aliquot overnight in 400 mL LB + carbenicillin medium at 30°C and performed three maxi preps and two mega preps (200 mL each) using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965, #12981). For the Upstream library ...
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bioRxiv - Biochemistry 2021Quote: ... 30 mg of sample were aliquoted in Eppendorf tubes and homogenized in 400 mL of methanol for 5 min at 25 Hz with a sample disruptor Qiagen TissueLyser II Laboratory Mixer (Qiagen, Valencia, CA, USA). The homogenized mixture was centrifuged for 10 minutes at 10000g (4 °C) ...
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bioRxiv - Neuroscience 2020Quote: ... HSB was removed and beads were re-suspended in 400 µL supplemented RLT buffer (10 µL β-mercaptoethanol/10 mL RLT Buffer) from the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) and vortexed vigorously ...
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bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... followed by lysis in a 2 mL sure-lock tube containing 5 mm stainless steel homogenization beads using the TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 seconds at 30 hz followed by 1 min of 30 hz while keeping the sample cold ...
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bioRxiv - Microbiology 2021Quote: ... Lung homogenates were prepared by homogenizing 200 mg of tissue in a tube containing 1 mL DMEM and a steel bead with a TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 s at 30 hertz ...
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bioRxiv - Neuroscience 2022Quote: PON2 level was quantified in the mouse whole brain homogenate in 100 mg per 1 ml of QIAzol® lysis reagent (Qiagen, Hilden, Germany). mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc. ...
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bioRxiv - Genetics 2020Quote: ... approximately 30 seeds per genotype were placed in a 2 mL Eppendorf Safe Lock tube along with one stainless steel bead (Qiagen Cat. No. 69989), frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... HSB was removed and beads were re-suspended in 400 µL supplemented RLT buffer (10 µL β-mercaptoethanol/10 mL RLT Buffer) from the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) and vortexed vigorously ...
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bioRxiv - Biochemistry 2021Quote: ... Medium was stirred for 1 hour at 4°C in the presence of 5 ml Ni-NTA Agarose beads (Qiagen, Venlo, The Netherlands) washed with 25 mM Hepes-KOH pH 7.5 ...
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bioRxiv - Immunology 2023Quote: Whole livers were harvested at indicated timepoints and placed into a 7 ml homogenizer tube (Omni International Cat. No. 19-651) containing 3 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Spleens and single granulomas were harvested at indicated timepoints and placed into a 1 ml homogenizer tube (Fisher Brand Cat ...
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bioRxiv - Genomics 2023Quote: ... The still frozen leaf powder was transferred into a 2.0 ml Eppendorf tube containing 400 µl API Qiagen extraction buffer (Qiagen DNeasy Plant Mini Kit). The mixture was incubated at 40°C for 1.5 hours with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were weighted using an analytical scale and homogenized in 1 ml of deionized water using TissueRuptor II electric homogenizer by Qiagen (Germantown, MA, USA). RO5256390 was extracted using acetonitrile/water 50%/50% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Plant tissue was homogenized in 1.5 ml microtubes with sterile plastic pestles and DNA was extracted using a DNeasy Plant Mini Kit (QIAGEN Sciences Inc., Germantown, MD) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspend the cells in 10 ml of suspension buffer (10 mM EDTA pH8.0, 150 mM NaCl, 1% glycerol, Lysis blue (1×, from QIAGEN Plasmid Plus Midi Kit), RNase A (0.55 mg/ml) ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was extracted from 50 ml cell culture medium using QIAamp Viral RNA Mini Kit according to protocol provided by the manufacturer (Qiagen, Cat. no. 52904). RNA concentration and quality were evaluated using NanoDrop 2000 Spectrophotometer (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2024Quote: Viral RNA was extracted from 50 ml cell culture medium using QIAamp Viral RNA Mini Kit according to protocol provided by the manufacturer (Qiagen; Cat. no. 52904). RNA concentration and quality were evaluated using NanoDrop 2000 Spectrophotometer (Thermo Fischer Scientific) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Lysates were incubated for 1 hr at 37°C after adding 10 µl of RNAse A (20 mg/ml) and proteins were precipitated with 200 µl of Protein Precipitation Solution (Qiagen Gentra Puregen Cell kit). After centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Whole ear tissues were homogenised in sterile 1.5 ml flat-bottom tubes containing 400 µl of PowerBead Solution (Part of DNeasy UltraClean Microbial Kit; Qiagen, Cat #12224-250, Hilden, Germany) with an OMNI Tissue Homogeniser with plastic hard-tissue probes (OMNI International) ...
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bioRxiv - Microbiology 2021Quote: ... 20–30 mg of tissue was taken and placed into a 2 mL tube with 600 µL of RLT buffer with 1% β–mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Hilden, Germany: #69989). Tissues were then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Pathology 2021Quote: ... 20 – 30 mg of tissue were placed into a 2 ml tube with 600 µl of RLT buffer with 1% β-mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Valencia, CA; #69989). Tissues were then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... with 5 µl proteinase K (20 mg/ml) and lysed overnight at 56°C under shaking in Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with proteinase K (20 mg/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysate was cleared by centrifugation (35,000 x g, 1 h) and the supernatant was loaded onto a Ni-affinity chromatography column (Qiagen Ni-NTA Superflow, 20 mL) pre-equilibrated with Ni-NTA buffer ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the remaining 2 mL of the cell suspension using the RNeasy® Protect Bacteria Mini Kit (QIAGEN, Cat. No. 74524) following the manufacturer instructions.
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... placed into a 2 mL tube with 600 μL of RLT buffer with 1% β–mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Hilden, Germany: #69989), then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a 10 mL culture using the QIAGEN® Genomic DNA Buffer Set and Genomic-tips™ 100/G set (midi-prep) (QIAGEN, Hilden, Germany). This procedure adhered to the Sample Preparation and Lysis Protocol for Bacteria as outlined in the QIAGEN® Genomic DNA Handbook ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were centrifuged at 16,000 g for 5 min and supernatant was treated with 4 µL of RNase A (100 mg/mL) (Qiagen Singapore Pte. Ltd, Cat. No. 19101), gently mixed by flicking of tube and incubated at room temperature for 2 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 µl buffer RLT plus with 2-Mercaptoethanol (βME) at 10 µl βME/ml of RLT+ was added before placing the tube into a TissueLyser LT (QIAGEN Germantown, MD, USA; Cat#69980) set at 50 oscillations/second for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 30 mg of frozen tissue was placed into a 2 ml flat bottom centrifuge tube on dry ice with a 5 mm stainless steel bead (QIAGEN Germantown, MD, USA; Cat#69989) at the bottom ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 µL of samples was mixed with Triton X-100 solution and then incubated with 25 µL of Proteinase K (ProK; Qiagen, 19131; 100 µg/mL in PBS) at 37°C for 30 min prior to Tluc measurement.
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bioRxiv - Cell Biology 2024Quote: ... were incubated in a 50 µL reaction with or without triton-x (1% v/v) and with or without proteinase K (Qiagen #19131, final concentration 20 µg/mL) for 15 minutes at room temperature ...