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Citations for Qiagen :
101 - 150 of 216 citations for Nickel oxalate dihydrate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Molecular Biology 2022Quote: ... added to 80 μl washed Ni-NTA agarose nickel beads slurry (Qiagen, Cat# 30230), and transferred to Pierce™ Spin Columns – Snap Cap (Thermo Scientific™ ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... S1 RBD was purified from the culture supernatant by nickel–nitrilotriacetic acid agarose (Qiagen), and purity was confirmed to by >95% as judged by coomassie stained SDS-PAGE ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were incubated with Nickel-nitrilotriacetic acid (NTA) sepharose (Qiagen, Hilden, Germany) at 4°C with slight shaking for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... coli Rosetta cells was carried out using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) as described in Barja and Rodríguez-Concepción (2020) ...
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bioRxiv - Molecular Biology 2021Quote: ... we added 70 µl of Ni-NTA (Nickel Nitrilo-triacetic Acid) agarose beads (Qiagen), and incubated the mixture overnight at the orbital rotator at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Nickel affinity chromatography was performed on the diafiltered supernatant with Ni-NTA agarose (Qiagen), eluting fractions with increasing concentrations of imidazole in TIM Buffer 1 ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 ml of culture supernatant was incubated with nickel-nitrilotriacetic acid-agarose beads (QIAGEN). The beads were washed ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was applied to a 5 ml nickel-nitrilotriacetic acid column (Qiagen, Valencia, CA) that had been equilibrated with buffer A at a rate of 1 ml/min ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified from filtered supernatants using nickel nitriloacetic acid (Ni-NTA) agarose (QIAGEN) via gravity flow ...
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bioRxiv - Molecular Biology 2023Quote: ... After purification of AtH2B.9 using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAGEN), the His6-tag portion was removed by TEV protease (30 µg/mg of His6-H2B.9) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The clarified lysate was incubated with nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (Qiagen) for 1 hour with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was loaded onto a nickel-nitriloacetic acid (Ni2+-NTA) column (Qiagen, Hilden, Germany), washed with 500ml buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was incubated with 3 ml nickel- nitrilotriacetic acid Ni-NTA beads (Qiagen) preequilibrated with the same buffer overnight ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were subjected to pull down using nickel-nitrilotriacetic acid (Ni-NTA) beads (Qiagen) overnight at 4 °C on a rotation platform ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was then mixed with 5 mL of nickel-NTA agarose resin (Qiagen) equilibrated with the lysis buffer ...
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bioRxiv - Neuroscience 2024Quote: ... for GST-tagged CMK-1 variants or nickel-nitrilotriacetic acid beads (Ni-NTA, Qiagen) in the presence of 15 mM imidazole for TAX-6-His6 protein binding ...
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bioRxiv - Biochemistry 2020Quote: ... Amylose elution pool was bound to Nickel NTA (NiNTA) affinity beads (Qiagen, Germantown MD, USA), washed with NiWB (500 mM Sodium Chloride ...
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bioRxiv - Synthetic Biology 2020Quote: ... The soluble fraction was passed through 3ml of nickel nitrilotriacetic acid agarose (Ni-NTA) (Qiagen), washed with 20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a nickel affinity column (Ni-NTA superflow columns from Qiagen) that was previously equilibrated with start buffer ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged AC was secreted into the medium and purified by nickel affinity chromatography (Qiagen) with elution in 100 mM citrate pH 4.0.
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bioRxiv - Biochemistry 2022Quote: ... Sso7dmut-sortase cleavage site-IN was purified with nickel affinity chromatography (Ni-NTA Superflow, Qiagen). The hexahistidine tag and Sso7dmut were cleaved from HIV-1 IN by addition of his-tagged sortase and GGGC peptide (GenScript) ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CSF3R protein was enriched by nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen 30210) and further purified by size-exclusion chromatography on a Superdex 75 10/300 GL column (Cytiva 17517401 ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Recombinant Fab proteins were purified from the culture supernatant by nickel-nitrilotriacetic acid agarose (Qiagen). Recombinant mAbs were affinity purified by MabSelect resin (Cytiva ...
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bioRxiv - Physiology 2024Quote: ... C3 constructs were purified from the soluble fractions by nickel-based affinity (Ni-NTA, Qiagen). The most concentrated fraction was further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatant was incubated end-over-end with 50 μl of NTA-nickel agarose beads (Qiagen) at 4°C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The clarified supernatant was loaded onto pre-equilibrated nickel-nitrilotriacetic acid (Ni-NTA) (Qiagen, USA) affinity column and flow-through was collected after incubation for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified by incubation with nickel-nitriloacetic acid (Ni-NTA) agarose resin (Qiagen) for 3h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Toxins were then purified from bacterial cell lysates through affinity chromatography on a Nickel-resin (Qiagen), ion exchange ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble lysate fraction was bound in batch to nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The resin was washed extensively with 20 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM PMSF and the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose (QIAGEN) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... lysed and incubated with nickel-nitrilotriacetic acid beads as per the manufacturer instructions (Qiagen, Hilden, Germany). Beads were washed ...
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bioRxiv - Biochemistry 2020Quote: ... Nickel affinity purification of 10His-SUMO1T95R conjugates was carried out with Ni-NTA agarose beads (Qiagen). 25μl beads were prepared per 1ml lysate by washing for three times with guanidine lysis buffer and added to the cell lysate for mixing overnight at 4□ ...
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bioRxiv - Molecular Biology 2020Quote: ... the collected eluate was incubated with pre-washed nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen) for 90 min at 4°C to remove the His-tagged TEV protease ...
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bioRxiv - Plant Biology 2022Quote: ... Purification was performed on nickel-nitrilotriacetic acid (Ni-NTA) agarose using the QIAexpressionist™ kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... coli under a T7 promoter and purified using Ni-NTA nickel affinity beads (Qiagen, Hilden Germany). Purified protein was dissolved in PBS solution and lyophilized ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Biochemistry 2024Quote: ... WT and mutant proteins were initially purified using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAgen). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... Proteins and complexes were purified from the cell supernatants using Nickel-NTA affinity resin (Qiagen: 30210), followed by Superose 6 (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... The solubilized suspension was then applied to a nickel-nitrilotriacetic acid agarose (Ni-NTA agarose, QIAGEN) column ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was supplemented with 10 mM imidazole pH 7.8 and incubated with nickel beads (Qiagen) for 10 – 30 min ...
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bioRxiv - Biophysics 2020Quote: ... which was performed by batch immobilized metal ion affinity (IMAC) using nickel agarose slurry (Ni-NTA, QIAGEN or Ni Sepharose High Performance ...
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Structural Basis for SARS-CoV-2 Envelope Protein in Recognition of Human Cell Junction Protein PALS1bioRxiv - Biochemistry 2021Quote: ... the supernatant was collected for affinity purification by nickel-nitrilotriacetic acid affinity chromatography (Ni-NTA, Superflow, Qiagen). The eluate was concentrated and buffer exchanged for tag removal by incubation with TEV protease overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 6xHis tagged protein were purified by immobilized metal ion chromatography using nickel-NTA super flow resin (Qiagen) as previously described with slight modifications46 ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was purified using either 10 mL nickel-chelating column or Strep-Tactin column (Qiagen). The N-terminal His-SUMO tags were cleaved from the eluted protein by TEV protease overnight and removed by passing through a second nickel column ...
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bioRxiv - Molecular Biology 2021Quote: ... The MBP eluate was then incubated with pre-equilibrated nickel-nitriloacetic acid resin (Ni-NTA agarose, Qiagen) for 60 mins in the presence of 20 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... and supernatants were collected for affinity purification over pre-equilibrated nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) columns ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 and 0.1 μg) were allowed to bind 30 μl of nickel-nitrilotriacetic acid-agarose beads (QIAGEN) for 1 hour after which unbound decoy was removed by washing with PBS ...
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bioRxiv - Microbiology 2023Quote: ... 5 ml of the supernatant were added to 1 ml of Nickel-NTA Agarose (Qiagen, Hilden, Germany) and incubated under continuous shaking at 4 °C for 1 h ...