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Citations for Qiagen :
1251 - 1300 of 1426 citations for Mouse Anti Chikungunya Virus E2 Protein 18H01 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was prepared from mouse and rat embryonic molar tooth RNA (extracted using RNeasy Plus Micro kit, Qiagen, Düsseldorf, Germany). cDNA constructs were inserted in TOPO II PCR-plasmids and using TOPO TA Cloning kit with chemically competent cells according to manufacturer’s protocol (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: Genomic DNA from 24 months old CreER and nmrHAS2 mouse livers was purified using a DNeasy Blood & Tissue Kit (Qiagen). One hundred nanograms of purified genomic DNA were used for the methylation measurement ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA from mouse embryos were isolated using RNeasy plus micro kit with on-column DNase I digestion method (QIAGEN). cDNA libraries were produced by using an Ovation RNAseq amplification kit Ver ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell-free DNA from cell culture supernatants and mouse plasma was manually isolated using the QIAamp MinElute ccfDNA Mini Kit (Qiagen), while cfDNA from patient plasma samples was isolated with QIAsymphony SP using the QIAsymphony DSP Circulating DNA Kit (Qiagen) ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: qRT-PCR was performed on total RNA extracted from adult mouse tissues or organoid cultures using the RNeasy Mini Kit (Qiagen). A DNAse I treatment (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from the human cell lines and mouse tumor samples was extracted using RNeasy Plus Mini Kits (Qiagen, 74134) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse brains with TRIzol or from selected CD11b or CD4-positive cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
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bioRxiv - Cancer Biology 2024Quote: Flash frozen mouse tissue was disrupted using an electric pestle and homogenized using a Qiashredder column (Qiagen, cat. no. 79654). Total RNA was extracted using the miRNeasy mini kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: Flash-frozen mouse tissue was disrupted using an electric pestle and homogenized using a Qiashredder column (Qiagen, cat. no. 79654). RNA was extracted using the miRNeasy mini kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: Total RNA from the developing mouse cerebral cortex and isolated GFP-positive cells were prepared using the RNeasy mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase (Thermo) ...
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bioRxiv - Developmental Biology 2024Quote: ... genomic DNA was extracted from mouse brain tissue and PCR amplifications were carried out with TopTaq Master Mix Kit (QIAGEN). PCR reactions of 25 μl were performed with 2 μl genomic DNA ...
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bioRxiv - Neuroscience 2024Quote: ... or the CA1 region of the mouse brain hippocampus (CA1 region was poured from both hippocampi) using RNeasy Micro kits (Qiagen). RNA purity was confirmed by BioAnalyzer ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from dorsal mouse skin was isolated from 5 control and 5 transgenic GRK2 eKO animals with RNAEasy Mini kit (#74104, Qiagen), following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Parasite genomic DNA was extracted from the mouse blood sampled after feeding the mosquitoes (Barcode input 2) using DNeasy Blood and Tissue Kit (Qiagen). The parasite gDNA was extracted from the infected mosquito midguts 14 days post-infection (Barcode output ...
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bioRxiv - Genetics 2019Quote: ... or anti-tetra His (0.1 μg/ml Qiagen 34670) were used as primary antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-His6 (34650; IF: 1/500) from Qiagen, mAb anti-GAPDH (GTX28245 ...
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bioRxiv - Microbiology 2020Quote: ... The anti-His antibody conjugated to horseradish peroxidase (Qiagen) was used at a dilution of 1:5,000 ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Anti-Penta-His antibody was purchased from Qiagen (Germany).
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bioRxiv - Biophysics 2023Quote: ... The antibodies were anti-RGSHis (Qiagen, Cat. No. 34650) and peroxidase-conjugated anti-mouse antibody (Dako ...
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bioRxiv - Genomics 2020Quote: ... for 4 hours to inhibit reporter transgene expression (t4) prior to isolation of DNA and RNA using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen# 80004) according to the manufacturers protocol ...
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bioRxiv - Genetics 2019Quote: ... protein altering inframe InDels (n=28) and missense variants (n=557) were uploaded to Ingenuity pathway Analysis (Qiagen, Venlo, The Netherlands). Additionally ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cancer samples were snap-frozen in liquid nitrogen and DNA extracted using the AllPrep DNA/RNA/Protein mini kit (QIAGEN, Germany) as previously reported 18 ...
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bioRxiv - Biochemistry 2020Quote: ... Canonical pathways associated with differentially expressed proteins were identified by Ingenuity Pathway Analysis (IPA, Ingenuity Systems, Qiagen, Redwood, CA, USA; www.ingenuity.com). This program is based on an algorithm that uses curated connectivity information from the literature to determine the network of interactions among the differentially expressed proteins and canonical pathways in which they are involved (Calvano et al. ...
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bioRxiv - Plant Biology 2021Quote: ... the cultured bacteria cells were lysed by using a high-pressure cell crusher and the fusion proteins were purified with Ni-NTA resin (Qiagen, www.qiagen.com) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein in the supernatant was purified to 90% purity by a single-step chromatography on Ni++ - metal chelate affinity (Qiagen, UK) columns and eluting at 0.5 M imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Matrix crystallization screens were performed at room temperature using the sitting drop vapor diffusion method by mixing equal volumes of protein sample and reservoir using a TTP LabTech Mosquito robot and commercially-available screens (Hampton Research and Qiagen). Initial hits were optimized and crystals were obtained in 20% PEG 3350 at 20 °C ...
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bioRxiv - Neuroscience 2020Quote: ... For the UCP4 antibody protein samples were extracted from the paraformaldehyde fixed tissue using the Qproteome FFPE Tissue Kit (Qiagen, Germany). The tissue blocks analyzed here were taken from the anterior cingulate and occipital cortex (as described above ...
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bioRxiv - Immunology 2022Quote: Initial matrix crystallization trials against 576 conditions were performed at room temperature using the sitting drop vapor diffusion method by mixing equal volumes of protein sample and reservoir using a TTP LabTech Mosquito robot and commercially-available screens (Hampton Research and Qiagen). Initial hits were optimized and crystals for unliganded BG24S60A were obtained in 0.25 M potassium chloride ...
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bioRxiv - Biophysics 2020Quote: ... Bacterial extracts were prepared as described46 and the recombinant protein was purified using an Ni-NTA Superflow cartridge (Qiagen, Hilden, Germany) and eluted with imidazol47 ...
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bioRxiv - Biochemistry 2019Quote: ... The soluble 6His-tagged proteins were purified from the supernatant by affinity chromatography using Ni2+-NTA agarose resin (Qiagen, Hilden, Germany). After several washing steps ...
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bioRxiv - Synthetic Biology 2020Quote: ... and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN, Germany) equilibrated with purification buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... assay targeting the paramyxovirus L protein gene was adapted using primers developed by Tong et al (36) and the Qiagen AllTaq PCR Core kit (QIAGEN, Australia). Amplicons corresponding to the expected size (584 bp ...
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bioRxiv - Cell Biology 2021Quote: ... Single cell clones were grown for immunoblot analysis and those showing loss of ATF4 protein were selected for sequence analysis involving the isolation of genomic DNA (Qiagen, 69504), PCR amplification using KOD Xtreme™ Hot Start DNA Polymerase (Millipore ...
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bioRxiv - Immunology 2021Quote: ... The plasmid was transfected to 293T cells and the recombinant S protein trimers were purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography (QIAGEN, Germany), followed by size exclusion to further purify the trimers ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from biopsies of normal colonic tissues with the Allprep DNA/RNA/Protein Mini kit (Qiagen, Germantown, MD) and reverse-transcribed into cDNA with the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... bacteria were pelleted at 3000 rcf and purified following the QiaExpress Ni-NTA Fast Start protein purification protocol (Qiagen, Catalog # 30600) under native conditions.
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bioRxiv - Biophysics 2023Quote: ... dCas9-Halo protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9 wash buffer I (50 mM NaxPO4 pH 7.0 and 300 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Cys protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9-Cys wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Cat# 28-9537- 66) was used and Streptavidin tagged proteins were purified using Strep-Tactin® Superflow® Plus cartridge (QIAGEN). Columns were attached to MINIPULS® 3 peristaltic pump (Gilson ...
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bioRxiv - Immunology 2023Quote: ... Functional pathway and network analysis of differentially expressed proteins were performed using Ingenuity Pathway Analysis (IPA) (Qiagen, Redwood City, CA, USA). The pathway and network score were based on the hypergeometric distribution and calculated with the right-tailed Fisher’s exact test.
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Frozen cells were thawed on ice and used for protein purification with nickel-nitrilotriacetic acid (Ni-NTA) Fast Start column as recommended by the manufacturer (Qiagen, Germany). The bound proteins were eluted from the column with elution buffer containing 50 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from soluble fraction under native conditions using Ni2+– NTA affinity chromatography (Ni2+ beads obtained from Qiagen, #30210), followed by a second step of purification involving gel filtration chromatography using Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... After incubation microstructures were washed with PBS and RNA was isolated with Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen # 80004). cDNA was synthesized using the SuperScript VILO cDNA synthesis kit (ThermoFisher #11755250) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested 4 days (96hr) after treatment and RNA extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, USA). Concentrations of RNA samples were analysed using Qubit fluorometric assays (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... The induction and purification of the recombinant protein was carried out under native conditions according to the QIAexpressionist manual (“Qiagen”, Germany). For the purpose of fusion protein purification from bacterial cells 1000 ml LB growth medium was inoculated by 25 ml of overnight E ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...