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Citations for Qiagen :
1251 - 1300 of 1498 citations for Diazinon Unlabeled 1000 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 1mM DTT and 100 µg/ml cycloheximide) and eluted in 350 µl of RLT Plus buffer (RNAeasy Plus Micro kit, Qiagen) supplemented with 2-mercaptoethanol (Bio-Rad).
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bioRxiv - Synthetic Biology 2023Quote: The pARP3 plasmid for methylation analysis was extracted from 200 mL of an overnight culture of Tol 5 transformant cells using the HiSpeed Plasmid Midi kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from five 10-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from ten 4-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Neuroscience 2024Quote: ... Overnight cultures of the positive clones were grown in LB Kanamycin (30 μg/mL) media and plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (Qiagen). Purified plasmid DNA was sequenced using ABI BigDye Terminator v3.1 Sequencing at the Australian with the following primers ...
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bioRxiv - Systems Biology 2024Quote: ... and left brain hemisphere using the phenol-chloroform extraction method with 1 mL of Qiazol Lysis Reagent (Qiagen, Hilden, Germany), as previously described in Ederer et al ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was centrifuged again at 5,000 x g for 20 minutes at 4°C and equilibrated for 1 hour in 3 mL of Ni-NTA resin (Qiagen) that was pre-equilibrated in the extraction buffer ...
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bioRxiv - Neuroscience 2024Quote: ... and 20 U/mL RNasin) and resuspended in 100 µL of RLT buffer from the RNeasy Micro Kit (Qiagen 74004) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Biophysics 2023Quote: ... The soluble extracts were applied to 2 ml columns of nickel-nitrilotriacetic acid agarose (Ni-NTA) (QIAGEN catalogue no. 30210) that had been equilibrated with lysis buffer ...
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bioRxiv - Plant Biology 2024Quote: ... The fresh leaf discs were transferred to a 2 mL tube containing a 5 mm stainless steel bead and 500 µL buffer RLT (QIAGEN). The leaf discs were disrupted using a TissueLyser LT (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... An 80 μL sample of the supernatant was transferred to a new tube and incubated with 5 μL of 5 mg/mL Proteinase K (QIAGEN) for 1 h at 60°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... resuspended in 50 ml of distilled water and the protocol was modified to include 0.1 mm glass beads (Qiagen, Germany) to increase DNA extraction by mechanical lysis ...
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bioRxiv - Microbiology 2024Quote: ... 10 Arabidopsis seedlings from the same MS plate were sampled together in 2 mL microtubes containing two 3 mm-diameter tungsten carbide beads (Qiagen), and flash frozen in liquid nitrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The bits containing the powder was immediately transferred and rinsed in a 2 ml Eppendorf (DNA/RNA and DNase/RNase free tube) containing 1 ml of Tissue Lysis buffer ATL from the QIAamp DNA Micro Kit (Qiagen) and stored at 4°C until the DNA extraction process (i.e ...
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bioRxiv - Genomics 2024Quote: ... The resulting lysate was then made up to 3 mL with QIAzol Lysis Reagent and mixed thoroughly before 1 mL lysate aliquots were processed using RNeasy Lipid Tissue Kit 74804 (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria are harvested by centrifugation (5 minutes, 4°C) and washed with 1 ml cold PBS containing RNA stabilization reagents (RNAprotect, Qiagen) before flash freezing and storage at -80°C ...
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bioRxiv - Biochemistry 2024Quote: ... RNAse A lysis buffer was prepared from IP buffer but is supplemented with 80 μg/mL of RNase A (Qiagen). Cells were gently resuspended and incubated for 30 minutes rocking on a tube rotator at 4°C ...
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bioRxiv - Microbiology 2023Quote: During this time a stock of genomic DNA (gDNA) from Escherichia coli (REL606) from ∼72 mL of overnight culture was made using the “DNeasy Ultraclean Microbial kit” (Qiagen), resulting in a stock of ∼30 mL of gDNA at 20 μg/mL (stored at -20 °C) ...
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bioRxiv - Microbiology 2023Quote: ... samples were transferred from DNA/RNA Shield – Lysis Tube to a 2 ml eppendorf and cells lysed using a TissueLyser II (Qiagen) at maximum speed (5 repetitions of 1 min with 1 min on ice between each) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated for 30 min at 4 °C with 5 mL of Ni2+-beads (Qiagen Ni-NTA Superflow) equilibrated in lysis buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteinase K 20mg/ml was added and incubated at 65 for 1 hour and DNA was purified with PCR purification Kit from Qiagen. Then ...
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bioRxiv - Microbiology 2023Quote: ... Then 4 μl RNase A (100 mg/ml) were added to the tubes followed by 150 μl of buffer AL (lysis buffer, Qiagen). After mixing by vortexing ...
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bioRxiv - Microbiology 2022Quote: ... transferred to 2 ml lysis matrix B tubes (MPBio) and subjected to bead beating for 15 min at 30 Hz (Tissuelyser II, Qiagen) with RNAse A added ...
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bioRxiv - Biochemistry 2023Quote: ... The pellets were resuspended with buffer A and loaded onto a 10 mL column of Ni-NTA agarose (Qiagen, Inc.) equilibrated with buffer A ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentrations of all the collected samples were adjusted to 400 μg mL−1 using the Qubit protein broad range assay kits (Qiagen). The Cyclic AMP XP® kit ELISA plates were prepared according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNAs were purified from 3 mL overnight cultures grew at optimum temperature (30 °C or 37 °C) by Dneasy Blood & Tissue Kits (QIAGEN). Additionally ...
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bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 37°C overnight in presence of a selective antibiotic Chloramphenicol (30 µg/ml) and isolated the final plasmid using a QIAprep Spin Miniprep kit (Qiagen) the following day ...
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bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 30°C overnight in presence of a selective antibiotic Ampicillin (100 µg/ml) and isolated the final plasmid via a Midiprep using a Qiafilater plasmid midi kit (Qiagen) the following day.
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bioRxiv - Microbiology 2023Quote: DNA was extracted from enrichment material in technical duplicates (1 ml digestate or 0.25 g soil) using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) with modified bead beating using a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...
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bioRxiv - Microbiology 2023Quote: ... We could not get a PCR product for the R escaper and therefore extracted the total DNA from the escaper and the ancestor λ-vir phage lysate after enrichment for high phage titer (> 107 pfu/ mL) DNA with the DNeasy blood and tissue kit (QIAGEN), starting from the Proteinase-K treatment step to lyse the phages 92 ...
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bioRxiv - Molecular Biology 2023Quote: The libraries were purified with a 30-minute incubation at 37 °C in the presence of 160 μg/mL RNase A (Qiagen), a treatment with 200 μg/mL Proteinase K (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... The homogenate was treated overnight with proteinase K (2 mg/ mL) at 56 °C and DNA was extracted using the MagAttract PowerSoil DNA EP Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: 7-day old in vitro grown plantlets or adult leaves of soil grown 3-4 week-old plants were ground in 2 mL tubes using a Tissue Lyser (Qiagen) twice for 30 sec at 30 Hz before RNA extraction using the RNeasy Plant Mini kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... a ∼20 mg chunk of frozen lung tissue was collected using a scalpel and placed in 1 mL RLT buffer (Qiagen) with 1:100 β-mercaptoethanol in a Precellys silicone bead dissociation tube (Bertin-Corp) ...
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bioRxiv - Biochemistry 2022Quote: Approximately 25 mg of frozen tissues were transferred to 2 mL Eppendorf Protein Lobind tubes containing one 5 mm stainless steel bead (cat# 69989, Qiagen) and 500 µL of lysis buffer consisting of 5% SDS in 50 mM TEAB with protease inhibitors cocktail (cat# A32953 ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL pre-equilibrated Ni-NTA resin (Qiagen) in a 50 mL falcon tube ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease40 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6int and CCR6high subsets of CD4+ T cells were used for isolating RNA without and with prior activation with 20 ng/ml PMA and 1 mM ionomycin for 3 h at 37°C under 5% CO2 using RNeasy Mini Kit (Qiagen) and gene expression levels were determined using Illumina Human WG6 Expression BeadChip Version 3 arrays (Illumina Inc. ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were homogenized in freshly prepared lysis buffer (0.6 ml per 30 mg of brain tissue) containing 1% β-mercaptoethanol using a TissueRuptor homogenizer (Qiagen).
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Physiology 2023Quote: ... were immersed in the Transnetyx microbiome sample collection tube (1.5 mL, prefilled with DNA stabilization liquid and glass beads) and sent to Transnetyx (Cordova, TN) for automated DNA extraction (Qiagen DNeasy 96 PowerSoil Pro QIAcube HT extraction kit) ...
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bioRxiv - Immunology 2023Quote: ... Plasmid DNA from overnight liquid cultures (LB with 100 mg/ mL ampicillin) was isolated with QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Synthetic Biology 2023Quote: ... The organs were weighed and lysed in 1 mL Triton X-100 solution (0.1% in PBS) using a TissueLyser II (QIAGEN, Germany) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: Investigation of the cell cycle related genes in LoVo cell line on treatment with FA FOS I was performed as described above by using the synchronized and 24 h FA FOS I (198 µg/ml) treated cells profiled by using RT2 Prolifer PCR Array- Human Cell cycle from Qiagen. The data was analysed by using Gene globe platform and reactome pathway analysis.
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bioRxiv - Microbiology 2022Quote: ... Bacterial cells were harvested from the surface of the cheese agar by using a sterile razor blade and were then immediately placed into 3 mL of RNAProtect Bacteria Reagent (Qiagen) and frozen at -80C until RNA extraction ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting total cell lysate was clarified by centrifugation (30,000 g for 25 min) and the supernatant fraction was applied to a 4 mL packed Ni-NTA resin (Qiagen) and gently rocked at 4 °C for 1.5 h in 50 mL conical tubes ...
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bioRxiv - Molecular Biology 2023Quote: cDNA was produced from 1 μg of total RNA (final cDNA concentration 5 ng/ml) using QuantiTech Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times with ice-cold PBS and lysed at room temperature with 1 ml/well QIAzol lysis reagent (QIAGEN). Lysates were incubated at room temperature for 5 min and homogenised by pipetting up and down ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were selected in puromycin (1 μg/ml) the next day and after 4 days genomic DNA was extracted using DNeasy Blood and Tissue kit (Qiagen). Genomic DNA was sonicated to an average size of 500 bp using S2 Focused-ultrasonicator (Covaris ...