Labshake search
Citations for Qiagen :
1251 - 1300 of 1943 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... 2 µg of purified genomic DNA was then bisulfite converted using the EpiTect Bisulfite Kit (Qiagen). PCR was performed on bisulfite-treated gDNA using HotStarTaq DNA polymerase (Qiagen) ...
-
bioRxiv - Genomics 2023Quote: ... PCR 2 products were purified via gel extraction using the Qiaquick Gel Extraction Kit (Qiagen #28706X4) and prepared for sequencing as described in Illumina’s library denaturation and dilution manual ...
-
bioRxiv - Microbiology 2023Quote: ... each spot was collected into a 1:2 mix of LBS and RNAprotect Bacteria reagent (Qiagen), incubated at RT for 5 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: Each qPCR reaction consisted of 5 μL of 2× QuantiNOVA SYBR Green PCR master mix (Qiagen), 0.5 μL of 10 μM forward and reverse primers each ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells sediment was added to 2 mL Red Blood Cells Lysis Solution (Qiagen, Duesseldorf, Germany) at room temperature for 5 min to remove red blood cells ...
-
bioRxiv - Systems Biology 2023Quote: ... About 1 OD/mL cells were mixed with 2 volumes of RNAprotect Bacteria Reagent (Qiagen, 76506), and the cell pellet was collected by centrifuging at 2500 g for 8 minutes at room temperature ...
-
bioRxiv - Plant Biology 2023Quote: ... Around 2 micrograms of DNA was used for DNA bisulfate conversion by EpiTect Bisulfate kit (QIAGEN). The converted DNA served as a template for amplification ...
-
bioRxiv - Neuroscience 2024Quote: Total RNA from iPSC-derived microglia (Supplementary Table 2) was extracted using RNeasy Plus kit (Qiagen). Library construction and sequencing were performed by Novogene ...
-
bioRxiv - Biochemistry 2023Quote: PCR products were purified from 2% agarose gel and extracted by QIAquick Gel Extraction Kit (Qiagen) following the manufacturer’s protocol.
-
bioRxiv - Microbiology 2024Quote: ... RNA was extracted from the Caco-2 cell lines using a RNeasy Mini Kit (QIAGEN SA) with a DNase I step to eliminate DNA contaminants ...
-
bioRxiv - Evolutionary Biology 2024Quote: Total SARS-CoV-2 RNA was extracted by using the Analytik Jena Kit (QIAGEN, Hilden, Germany), followed by reverse transcription into cDNA with a high capacity cDNA reverse transcription kit (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2024Quote: ... DNA was extracted from 1 or 2 bumblebee legs using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... and dact1/2-/-fish were collected and RNA extractions were performed using RNeasy Mini Kit (Qiagen). cDNA was generated using High Capacity cDNA Reverse Transcription Kit (ThermoFisher) ...
-
bioRxiv - Biophysics 2020Quote: ... functionalized with neutravidin and treated with biotinylated anti-hexahistidine monoclonal antibody (Qiagen) as described (15) ...
-
bioRxiv - Biochemistry 2021Quote: ... Electroblotted membranes were incubated with penta-His antibody (Qiagen, Valencia, CA, USA). The antigen–antibody complex was visualized with alkaline phosphatase-linked to anti-rabbit IgG followed by staining with 5-bromo-4-chloroindol-2-yl phosphate and nitro blue tetrazolium [36].
-
bioRxiv - Microbiology 2021Quote: ... The followed antibodies and dilutions were used: Strep 1:1000 (Qiagen, 34850), PAF1 1:1000 (Bethyl Labs #A300-173A) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tags ...
-
bioRxiv - Biophysics 2020Quote: ... the surface was incubated with 1 nM biotinylated penta-His antibody (Qiagen) in buffer A for 10 min ...
-
bioRxiv - Biochemistry 2021Quote: ... The primary and secondary antibodies used were anti-penta His (Qiagen #34660) and goat anti-mouse IgG (LiCOR #926-68070 ...
-
bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tag ...
-
bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse penta-His antibody (cat. no. 34660, QIAGEN) against His tags ...
-
bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 μL PBS containing 0.1 μg anti-penta·His antibody (Qiagen) and 1 μg streptavidin allophycocyanin (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... and immunoblotted using a mouse monoclonal antibody against Strep-tag (Qiagen, 34850). Alkaline phosphatase conjugated to anti-mouse IgG (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: Mouse antibody genes were amplified using HotstarTaq DNA polymerase (Qiagen Cat # 203209) with the primer sets specific for the IghIOMAiGL and IgkIOMAiGL transgenes ...
-
bioRxiv - Biochemistry 2023Quote: ... a conjugate of mouse monoclonal penta-His antibody and horseradish peroxidase (Qiagen) was used ...
-
bioRxiv - Genomics 2021Quote: Genomic DNA from MCF7 cells was isolated by proteinase K digestion at 65°C for 30 minutes followed by purification using the QIAamp circulating nucleic acid kit (Qiagen, Venlo, The Netherlands). Genomic DNA from frozen tissue sections of colorectal liver metastases was isolated using the NucleoSpin Tissue kit according to the manufacturer’s guidelines ...
-
bioRxiv - Molecular Biology 2020Quote: ... was digested overnight at 56°C in 20 ul proteinase K and 180 ul Buffer ATL from the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Valencia, California, USA). DNA was extracted from the digest with the QIAamp PowerFecal DNA Kit (Qiagen ...
-
bioRxiv - Physiology 2019Quote: ... to remove the cell debris and were stored at −80°C for no longer than 4 weeks until isolation of cf DNA with QIAamp DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany) and measurement of plasma concentrations of cf n-DNA and cf m-DNA with real time quantitative PCR as described previously3 ...
-
bioRxiv - Genetics 2020Quote: ... we grew one aliquot overnight in 400 mL LB + carbenicillin medium at 30°C and performed three maxi preps and two mega preps (200 mL each) using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965, #12981). For the Upstream library ...
-
bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
-
bioRxiv - Microbiology 2021Quote: ... Filters were flash-frozen in liquid nitrogen and stored at (−80°C) until extraction of DNA using the DNeasy Blood and Tissue Kit (cat. 69504 QIAGEN, Germantown, MD, USA) combined with QIAshredder (cat ...
-
bioRxiv - Microbiology 2021Quote: ... Buffer G2 and proteinase K were incubated at 56°C overnight and extracted using the EZ1 DNeasy Blood Tissue Kit (Qiagen GmbH, Hilden, Germany) in line with the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2020Quote: Data to derive different biological clocks was available for different subsamples and all based on a fasting blood draw from participants in the morning between 8:30 and 9:30 after which samples were stored in a −80°C freezer or – for RNA - transferred into PAXgene tubes (Qiagen, Valencia, California, USA) and stored at −20°C ...
-
bioRxiv - Cell Biology 2022Quote: ... The reaction mixture was incubated at 37°C for 24 hours and subsequently underwent RNA clean-up using a column-based purification (Qiagen, product number 74104). The purified RNA was eluted in nuclease-free water (Qiagen ...
-
bioRxiv - Cell Biology 2020Quote: ... Efficient electroporation was confirmed by observation of fluorescence before genomic DNA extraction around 16 hpf (18°C) using QIAamp DNA Micro kit (Qiagen, German Town, MD). Mutagenesis efficacy of individual sgRNAs ...
-
bioRxiv - Neuroscience 2021Quote: ... The mixture was incubated at 37 °C for various times and the DNA was purified using a MinElute PCR purification kit (Qiagen, Germantown, MD, USA). Then ...
-
bioRxiv - Molecular Biology 2022Quote: ... and the DNA pellet was stored at −80°C until purification was performed using a commercial kit (DNeasy Blood and Tissue Kit, catalog #69504; Qiagen; Germantown, MD, USA) according to manufacturer’s instructions with a minor modification to the elution step ...
-
bioRxiv - Microbiology 2022Quote: ... strain SPR117 was grown during 7 days in R2A broth at 20 °C and its genomic DNA (gDNA) was extracted using the Dneasy UltraClean Microbial Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Brain parts were stored in 100 µL of Trizol™ in -80 °C for later RNA extraction using RNAeasy Mini Extraction Kit™ (Qiagen, Germany) according to the manufacturers protocol ...
-
Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... The supernatant was transferred to clean 1.5mL MCF tube and to which 350 μL ethanol at 4’C was added before vortexing for 10 sec and transferring the supernatant to a QIAamp Mini spin column (Qiagen Germantown, MD U.S.A.). The sample was process as recommended in the QIAamp DNA Mini Handbook and eluted with 10 mM Tris-Cl ...
-
bioRxiv - Microbiology 2019Quote: ... from the bacterial cell pellet of input library and output libraries stored at −80°C was extracted using QIAamp DNA Mini Kit (Qiagen, Valencia, CA, USA) following manufacturer’s protocol ...
-
bioRxiv - Genomics 2021Quote: ... individuals were flash frozen in liquid nitrogen and stored in −80°C until DNA extraction using the Qiagen DNeasy Plant kit (Qiagen, Valencia, CA, USA) as described in (Kainer et al ...
-
bioRxiv - Biochemistry 2021Quote: ... Medium was stirred for 1 hour at 4°C in the presence of 5 ml Ni-NTA Agarose beads (Qiagen, Venlo, The Netherlands) washed with 25 mM Hepes-KOH pH 7.5 ...
-
bioRxiv - Microbiology 2022Quote: ... The soluble fraction was separated from debris by centrifugation (9000 g, 4°C) and incubated for 60 minutes with Ni-NTA beads (Qiagen, cat. no. 30230) pre-equilibrated in binding buffer ...
-
bioRxiv - Microbiology 2023Quote: ... approximately 1 x 104 colonies were collected and pooled into LB-amp culture media and then incubated at 37°C for five hours prior to harvesting the bacterial population for plasmid purification by Maxiprep (Qiagen Plasmid Maxi Kit). The presence of a diverse barcode in the plasmid stock was verified by next generation sequencing (see below) ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
-
bioRxiv - Molecular Biology 2024Quote: Total bacterial DNA was extracted from frozen (−80°C) fecal samples from AuPhos- or vehicle treated mice using PowerFecal DNA/RNA Kit (Qiagen, Hilden, NRW, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were kept at −80°C until performing the DNA extraction using DNAeasy Blood & Tissue Kit (QIAGEN; Cat. No. ID: 69504). Qubit Broad Range double-stranded DNA assay (Life Technologies ...
-
bioRxiv - Physiology 2023Quote: Isolated human LGR5+ cells were stored at −80°C until processed for RNA isolation using RNeasy micro kit as per manufacturer instruction (Qiagen, Germantown, MD, USA). RNA processing ...
-
bioRxiv - Biophysics 2024Quote: ... for 30 min at 4 °C before being applied to a 10mL bed volume Nickel nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, cat. no. 30230) column ...