Labshake search
Citations for Qiagen :
1201 - 1250 of 1536 citations for Ferrostatin 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: SARS-CoV-2 RNA was harvested from the cell medium at various time points post infection and stored in viral lysis buffer with RNA supplements (50 μl of sample medium in 560 μl of AVL buffer supplemented with 1% carrier RNA) (Qiagen). RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was centrifuged at 10000g for 10 minutes and the supernatant was allowed to bind with 1 ml of Ni-NTA beads (Qiagen) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... A 1 μg of total RNA of each sample was reverse-transcribed to cDNA using RT2 first strand kit (Qiagen) according to manufactures protocol ...
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bioRxiv - Microbiology 2022Quote: ... Tissue sections collected for virology were disrupted in 1 mL of sterile PBS using the TissueLyser LT (Qiagen, Germantown, MD) at 30 Hz for 5 min twice ...
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bioRxiv - Biophysics 2022Quote: ... The resulting mixture was rotated at 4 °C for 60 min and then poured into a 1 mL polypropylene column (Qiagen). The resin was washed three times with 5 mL lysis/wash buffer and eluted in 0.25 mL fractions with Ni-NTA elution buffer (composition same as lysis/wash buffer ...
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bioRxiv - Immunology 2022Quote: ... The cDNA libraries (1 ng) were tagmented with the prepared single-loaded Tn5 transposase and afterwards cleaned using MinElute PCR kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from 1-2*106 sorted naïve (CD25− CD44lowCD62high) or effector (CD25− CD44highCD62low) CD4+ T-cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 1 μg of RNA was used as input for cDNA synthesis reactions by using the Omniscript RT Kit (Qiagen) along with random hexamers ...
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bioRxiv - Molecular Biology 2024Quote: ... The fidelity of the PCR reaction was checked using a 1 % agarose gel and then the reaction cleaned up using a PCR clean up spin column kit (Qiagen). Finally the concentration of the purified PCR product was checked on a NanoDrop spectrophotometer (Thermo-Fisher).
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bioRxiv - Cell Biology 2024Quote: ... The genomic DNA fragment was separated by electrophoresis using a 1% agarose gel and purified using QIAquick Gel Extraction Kit (QIAGEN). The genome sequence of drp-1 was determined by Sanger sequencing using primers listed in Supplementary Table S2.
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bioRxiv - Biochemistry 2024Quote: ... The cell pellet was removed after centrifugation (16000 rpm, 4 °C, 1 h) and supernatant was loaded into a Ni-NTA agarose column (Qiagen). Protein was eluted in an elution buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated RNA was retro-transcribed to cDNA using 1 μg of RNA per reaction with either the QuantiTect Reverse Transcription Kit (205311, QIAgen) or the High-Capacity cDNA Reverse Transcription Kit (4368813 ...
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bioRxiv - Microbiology 2024Quote: ... while samples for RNA-based analyses were mixed at a 1:10 ratio of LifeGuard Soil Preservation (Qiagen, Hilden Germany) before being put on dry ice ...
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bioRxiv - Systems Biology 2024Quote: ... To this 200uL of resuspended pellet we added 1 volume (200uL) of the QX1 buffer from the Qiaex2 DNA extraction kit (Qiagen) [56] ...
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bioRxiv - Microbiology 2024Quote: ... one fecal pellet per procedure was placed in a 2 mL round-bottom tube containing a 0.5 cm diameter stainless steel bead and 500 µL of sterile phosphate-buffered saline (PBS) and agitated at 1/30s using a TissueLyser II (Qiagen) for 60 seconds ...
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bioRxiv - Neuroscience 2024Quote: ... DRG and paw skin RNA was extracted using a RLT buffer:2-mercapto ethanol mixture in a 100:1 ratio/RNeasy (Qiagen) RNA mini kit according to the manufacturer’s instructions and spinal cord and spleen RNA was extracted using TRizol (Invitrogen)/RNeasy RNA mini kit ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The bits containing the powder was immediately transferred and rinsed in a 2 ml Eppendorf (DNA/RNA and DNase/RNase free tube) containing 1 ml of Tissue Lysis buffer ATL from the QIAamp DNA Micro Kit (Qiagen) and stored at 4°C until the DNA extraction process (i.e ...
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bioRxiv - Genetics 2024Quote: ... and a negative control region within a regulatory region of the estrogen receptor substrate 1 (ESR1) without a predicted G4 motif (91) were PCR-amplified with HotStar Taq Polymerase (Qiagen). The PCR fragments were cleaned-up with Promega ReliaPrep DNA concentration kit and eluted in dH2O ...
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bioRxiv - Developmental Biology 2024Quote: ... thresholds were used to prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries using the Ovation RRBS Methyl-Seq System 1-16 (NuGen) and the EpiTect Fast DNA Bisulfite kit (Qiagen). Libraries were sequenced using the NextSeq500 platform (Illumina ...
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted to 5 ng/µl in RNase-free water and 1 µl used for real-time qPCR with Qiagen QuantiNova SYBR Green Master Mix (Qiagen) in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: Total genomic DNA was extracted from 10% suspension of fin clips (<1 mm2) using the DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: ... The resulting lysate was then made up to 3 mL with QIAzol Lysis Reagent and mixed thoroughly before 1 mL lysate aliquots were processed using RNeasy Lipid Tissue Kit 74804 (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were directly lysed in the well using 350 µL of lysing solution (1% 2-mercaptoethanol in Buffer RLT; RNeasy Mini Kit, 74106, Qiagen) by pipetting over the well ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria are harvested by centrifugation (5 minutes, 4°C) and washed with 1 ml cold PBS containing RNA stabilization reagents (RNAprotect, Qiagen) before flash freezing and storage at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... HsMX1 or MmMx1 and infected or not with IAV at MOI 1 were extracted using the miRNeasy Mini kit (Qiagen). RNA quality was analysed using a BioAnalyzer (Agilent ...
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bioRxiv - Microbiology 2024Quote: 0,5-2 × 106 cells were collected 8 h or 24 h following mock infection or infection with A/Victoria/3/75 infection at MOI 1 and total RNAs were isolated using the RNeasy kit with on-column DNase treatment (Qiagen). cDNAs were generated using 125 ng RNA (High-Capacity cDNA Reverse Transcription Kit using 250 nM oligodT instead of the provided random primers ...
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bioRxiv - Microbiology 2024Quote: ... corresponding to the three extraction protocols we used: (NM-1) one negative control for the kit DNeasy Blood and Tissue (Qiagen) used for mosquito DNA extraction ...
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bioRxiv - Microbiology 2023Quote: ... At the last passage parasite pellets with ∼1 × 108 parasites were collected for DNA isolation using DNeasy blood and tissue kit (Qiagen).
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bioRxiv - Cell Biology 2023Quote: ... RNA (2 μg per sample) extracted from 1×106 BSCs was performed using the Qiagen RNA RNeasy Kit (Cat#74104, Qiagen) followed by library preparation with the Illumina TruSeq V2 Kit (Cat#20020594 ...
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bioRxiv - Genetics 2023Quote: ... To prepare the protein column we loaded 2 mL (1 mL column volume) of Nickel NTA resin onto the 5 mL Polypropylene columns from Qiagen and the resin buffer was allowed to drain ...
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bioRxiv - Genomics 2023Quote: ... tubes were incubated at 56°C for 1 hour followed by addition of 2ul of RNAse A (100mg/ml, QIAGEN) to 1ml of saliva sample and incubated at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 1× PBS and total DNA was extracted at 6 hpi with DNeasy Blood/Tissue DNA mini kit (Qiagen) and quantitated by a nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2023Quote: ... Cerebrum from the alligator snapping turtle and brainstem from the loggerhead turtle were homogenized in 450 μL 1× phosphate-buffered saline (PBS) by using a TissueLyser LT (Qiagen) at 35 Hz for 2 minutes with a sterile stainless steel 0.5 mm metal bead (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 RNA in plasma or in the primary viral isolates was extracted using the QIAamp Viral RNA Mini Kit (Qiagen). cDNA was synthesized using the SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... villosum) (Table 1) were used in RT-PCR tests conducted in Slovenia using the OneStepTM RT-PCR kit (Qiagen, USA) as previously described (Rivarez et al ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...
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bioRxiv - Microbiology 2023Quote: ... RNA was eluted from the column in RNase/DNase free H2O and any residual DNA contamination was removed with a 1 hour off-column DNase treatment (Qiagen). The RNA was purified following the RNeasy Mini-Prep Kit following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteinase K 20mg/ml was added and incubated at 65 for 1 hour and DNA was purified with PCR purification Kit from Qiagen. Then ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were resolved on 0.5x Tris-acetate-EDTA (TAE) 1 % agarose gels and the corresponding bands were cut out and purified using the QIAquick Gel Extraction kit (Qiagen). Restriction enzymes and T4 DNA ligase were obtained from NEB and Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... TetON-plko constructs were digested using AgeI and EcoRI restriction enzyme and the 8.8 kb fragment was purified from a 1% agarose gel using the Qiagen Gel Extraction kit (Qiagen, 28704). Annealed shRNA oligos and digested TetON-pLKO constructs were mixed together with T4 DNA Ligase (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The digested product was loaded on a 1% agarose gel and purified from the gel with PCR and gel purification kit (# 28506 Qiagen). The insert was ligated into the vector with a ratio of 3:1 overnight at 16°C using T4 Ligase (# 300361 Bioconcept) ...
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bioRxiv - Cell Biology 2023Quote: ... Amplicons were resolved on 1% agarose gel by gel electrophoresis and purified using the QiaQuick Gel Extraction Kit (QIAGEN #28706). Purified PCR product was cloned into the TOPO TA Vector and transformed into OneShot TopTen Chemically Competent E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... previously coated with the anti-B55δ antibody (1 hour incubation at 4°C, followed by extensive washes) and Nickel beads (Qiagen) coated with 400 μg of 6XHIS-S67thio-S109A-XeARPP19 ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was mixed with an equal volume of chloroform/isoamyl alcohol (24:1) and separated with a phase-lock tube (Qiagen) by centrifugation (14000 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... TbFUT1 cKO and TbFUT1 cKO F1-γL262P/WT cells grown under permissive and non-permissive conditions and RNA was extracted from 1 ×107 cells using RNeasy RNA extraction kit (Qiagen). RT-qPCRs were performed using Luna® Universal qPCR Master Mix (NEB ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from enrichment material in technical duplicates (1 ml digestate or 0.25 g soil) using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) with modified bead beating using a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...
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bioRxiv - Microbiology 2023Quote: ... viral nucleic acids were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany), which purifies RNA and DNA ...
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
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bioRxiv - Genomics 2023Quote: ... The samples were rinsed with 4× SSC and hybridized with RNA seqFISH primary probe pools (1-10 nM per probe) and 10 nM polyT LNA oligo (Qiagen) in a 50% hybridization buffer consisting of 50% formamide (Invitrogen AM9342) ...