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Citations for Qiagen :
951 - 1000 of 1444 citations for Mouse Anti Hepatitis C Virus NS3 Antibody 1828 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Template DNA was degraded using RQ1 DNAse for 30 min at 4°C and RNA was purified using RNAeasy mini kit (Qiagen). 4-week-old NRG mice were injected intra-hepatically using 10 µg of RNA in a maximum volume of 50 µL of PBS+RNA and one week post injection a terminal bleed was performed ...
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bioRxiv - Molecular Biology 2023Quote: ... CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen). Extracted gDNA was stored at −20°C.
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bioRxiv - Neuroscience 2023Quote: ... The remaining cells were pelleted at 1000 x g for 10 minutes at 4°C and lysed in 350 µL of Buffer RLT (Qiagen) supplemented with 3.5 µL of 2-β mercaptoethanol (#444203 ...
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bioRxiv - Microbiology 2023Quote: ... an unbiased amplification method was employed by amplifying the purified DNA at 30°C for 8 hours using a REPLI-g Single Cell kit (Qiagen). The amplified DNA was purified using AMPure XP magnetic beads (Beckman Coulter ...
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Sweetwater: an underrated crude glycerol for sustainable lipid production in non-conventional yeastsbioRxiv - Systems Biology 2023Quote: ... tubes were vortexed for 15 s and submitted to cell disruption for 15-min at 4°C using a TissueLyser II (Qiagen) at 30 Hz ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was aspirated out and the pellets stored at −80°C until mRNA extraction using the RNEasy Micro Kit (Qiagen). Corresponding cDNA was synthesized from the mRNA samples using the cDNA amplification kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 5 mM DTT) and incubated for 45 minutes at 65°C before addition of 40 μg Proteinase K (Qiagen) and further incubation for 1.5 hours at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Pathology 2023Quote: ... tissue and cells were lysed in RIPA lysis buffer supplemented with orthovanadate and a cocktail of protease inhibitors at 4°C (using a TissueLyser (Qiagen) to homogenize liver tissue ...
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bioRxiv - Genetics 2023Quote: ... Digests were incubated for 2 hours at 37°C then purified using a QiaQuick PCR purification kit (Qiagen Cat#28106). Fragments of 2100 bp were size-selected using a SageELF instrument (Sage Science ...
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bioRxiv - Genomics 2023Quote: ... tubes were incubated at 56°C for 1 hour followed by addition of 2ul of RNAse A (100mg/ml, QIAGEN) to 1ml of saliva sample and incubated at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were centrifuged for 30 min at 38,000 g and 4°C and cleared supernatants loaded onto Ni-NTA columns with 0.5 mL bed volume (1018244 Qiagen, Germany). The columns were washed sequentially with 3 column volumes (CV ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting samples were supplemented with 300 mM NaCl and 10 mM imidazole and incubated overnight at 4 °C on a stirring wheel with 50 µl Ni-NTA agarose resin slurry (QIAGEN) pre-equilibrated with wash buffer I (50 mM Tris-HCl pH 7.8 ...
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Phenogenomic resources immortalized in a panel of wild-derived strains of five species of house micebioRxiv - Evolutionary Biology 2023Quote: Genomic DNA was isolated from frozen (−80 °C) or alcohol-preserved muscle or spleen tissues using DNeasy Blood & Tissue Kits (Qiagen). High-quality DNA aliquots were next-generation sequenced at the Edinburgh Genomics facility using HiSeq X technology ...
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bioRxiv - Biochemistry 2023Quote: ... shaking overnight at 30°C for 16hrs and the plasmid DNA is purified using a QiaPrep 96 column DNA purification kit (Qiagen). This procedure was conducted twice on different days to produce two separate replicates.
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow of transplanted tumor bearing CTR and VEGF-C mice was isolated and gDNA was purified using QIAmp DNA Mini Kit (Qiagen) as described ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37 °C with agitation for six hours after which time 1 mL cell pellets were treated with RNAprotect (Qiagen). Cell pellets were washed in 0.5 mL PBS pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Then samples and inputs were incubated for 4 hr at 37°C with 1.5 µl of 10 mg/ml RNase A (Qiagen, 1007885) and 15 µl 10% sodium dodecyl sulfate and 3.5 µl 20 mg/ml Proteinase K (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We collected three biological replicates of first molars from both species at three postnatal stages (P1, P15, and P21) and immediately preserved them at −80°C in lysis buffer (Buffer RLT; Qiagen) supplemented with 40 μM dithiothreitol ...
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bioRxiv - Microbiology 2024Quote: ... the bacterial pellet was either stored at −80°C for further RNA extraction [after incubation in RNAProtect (Qiagen, Hilden, Germany) for 10 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysates were centrifuged at 10000 g for 30 min at 4 °C and the clear supernatant was collected and passed through Ni-NTA agarose column (Qiagen). Contaminating proteins were washed away by passing a 20–120 mM imidazole gradient through the column while the bound toxins were eluted using PBS containing 500 mM imidazole ...
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bioRxiv - Neuroscience 2024Quote: ... were either stored at -80°C or directly processed for total RNA extraction by using the miRNeasy Micro Kit (cat#: 74004; Qiagen) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction mixture was incubated at 37°C for 1 hour followed by purification using QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted from whole blood samples for all 256 samples at the Eijkman Institute for Molecular Biology Jakarta using the Gentra Puregene Blood Core Kit C (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... was performed for 30 min at 37 °C and the resulting fragments are purified using MinElute PCR Purification Kit (Qiagen). qPCR was performed to determine the optimal number of cycles necessary for each library ...
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bioRxiv - Molecular Biology 2021Quote: ... miRCURY LNATM miRNA inhibitor for anti-miR20a-5p and anti-miR149-5p were bought from Qiagen. Total RNAs were extracted 24 hours or 96 hours (for anti-miR test ...
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bioRxiv - Cell Biology 2020Quote: ... anti-RGS-His6 (BSA-free; QIAGEN), anti-calnexin (M178-3 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-miR-221-3p (Qiagen MIN0000278) or non-targeting siRNA (NT ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA from human and mouse kidney samples was harvested using the RNeasy Mini Kit (Qiagen). Total RNA isolation from cultured cells was extracted using Trizol reagent (Ambion ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from cells or mouse skin using the RNAeasy Plus universal kit (Qiagen 73404). RNA was quantified by absorbance at 260 nm ...
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bioRxiv - Genetics 2021Quote: Total RNA was isolated from mouse aortas or cultured cells using an RNeasy mini kit (QIAGEN), per the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: RNA from mouse aortas was extracted after mechanical homogenization of the aorta in lysis buffer (Qiagen) using Triple-pure zirconium beads and a microtube homogenizer (Benchmark Scientific ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from mouse tissues and single cell suspensions using RNeasy Plus kits (Qiagen). cDNAs were generated using SuperScript II (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was prepared from human or mouse B cells using QIAmp DNA Mini Kit (Qiagen), or from paraffin-embedded human IgD or IgA myeloma tissue sections (obtained from the University of Arkansas for Medical Science ...
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bioRxiv - Microbiology 2021Quote: ... DNA of the mouse pool (MP) was isolated using a QIAamp PowerFecal Pro DNA Kit (Qiagen).
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bioRxiv - Microbiology 2019Quote: Bacterial DNA extraction from mouse gut was performed with a QIAamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... mouse vagal ganglia were removed and placed in sterile cell lysis buffer (Qiagen Puregene, Hilden Germany) with 0.5% SDS and 100ug/ml Proteinase K at 55°C overnight ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from mouse or human whole blood using RNA blood mini kit (Qiagen). Isolated RNA was converted to complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from cultured human and mouse cells using an RNeasy Mini Kit (Qiagen) and included an on-column DNase treatment to eliminate contaminating genomic DNA ...
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was extracted from mouse kidneys using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Quantitative real-time-PCR was performed using RT2 Profiler PCR Array for Mouse Mitochondria (330231, Qiagen) with PowerUp SYBR Green (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from mouse carotid artery or HAECs using RNeasy Plus Micro Kit (QIAGEN, 74134) and 200 ng of total RNA was subjected to reverse transcription reactions using iScript™ Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... total RNA was isolated from mouse left ventricles using the Qiagen AllPrep Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial DNA and genomic DNA were isolated from mouse hearts using DNeasy Blood & Tissue Kits (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: RNA was isolated from zebrafish or from mouse tissue using the RNeasy Plus Micro Kit (Qiagen) and was used to prepare cDNA using a commercial kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from whole mouse lung tissue using the RNeasy Spin column kit (Qiagen #75144) then reverse transcribed using the cDNA Reverse Transcription Kit (Applied Biosystems #4308228 ...
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bioRxiv - Neuroscience 2022Quote: ... Genomic DNA was isolated from Mouse Embryonic fibroblast cells (MEF) using QIAmp DNA mini kit (Qiagen), PCR amplified and subsequently cloned in vector pEGFPC1 with a constitutive promoter ...
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bioRxiv - Pathology 2022Quote: ... Total RNA of mouse and human intestinal tissue was isolated using the RNeasy Mini Kit (Qiagen) and total RNA of mouse mesenteric fat was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen) ...
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bioRxiv - Systems Biology 2023Quote: ... RNAs were extracted from isolated mouse SAN with RNeasy Mini Kit (Qiagen, Valencia, CA, United States) and DNAse ...