Labshake search
Citations for Qiagen :
951 - 1000 of 1417 citations for Chikungunya Virus E2 Envelope Protein HEK293 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... Quantitative real-time-PCR was performed using RT2 Profiler PCR Array for Mouse Mitochondria (330231, Qiagen) with PowerUp SYBR Green (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from mouse carotid artery or HAECs using RNeasy Plus Micro Kit (QIAGEN, 74134) and 200 ng of total RNA was subjected to reverse transcription reactions using iScript™ Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... total RNA was isolated from mouse left ventricles using the Qiagen AllPrep Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial DNA and genomic DNA were isolated from mouse hearts using DNeasy Blood & Tissue Kits (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: RNA was isolated from zebrafish or from mouse tissue using the RNeasy Plus Micro Kit (Qiagen) and was used to prepare cDNA using a commercial kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from whole mouse lung tissue using the RNeasy Spin column kit (Qiagen #75144) then reverse transcribed using the cDNA Reverse Transcription Kit (Applied Biosystems #4308228 ...
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bioRxiv - Neuroscience 2022Quote: ... Genomic DNA was isolated from Mouse Embryonic fibroblast cells (MEF) using QIAmp DNA mini kit (Qiagen), PCR amplified and subsequently cloned in vector pEGFPC1 with a constitutive promoter ...
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bioRxiv - Pathology 2022Quote: ... Total RNA of mouse and human intestinal tissue was isolated using the RNeasy Mini Kit (Qiagen) and total RNA of mouse mesenteric fat was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: ... PCR arrays were performed using RT2 Profiler™ PCR Array Mouse Cytokines & Chemokines (PAMM-150Z, Qiagen). Calculations were performed by a comparative method (2−ΔΔCt ...
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bioRxiv - Microbiology 2022Quote: Mouse fecal pellets of approximately 0.1g were homogenized and processed using the DNA PowerSoil kit (Qiagen) with the QIACube automated platform following the manufacturer’s inhibitor removal technology (IRT ...
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bioRxiv - Genetics 2023Quote: ... Mouse Ahrr and Cyp1a1 primers were generated using the PyroMark Assay Design software version 2.0 (Qiagen). Primer sequences can be found in Table S1 ...
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bioRxiv - Pathology 2023Quote: ... Microbial DNA was isolated from mouse fecal samples (QIAamp PowerFecal Pro DNA Kit; Qiagen, Hilden, Germany). At the end of the experiment ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was prepared from mouse embryonic molar tooth RNA (extracted using RNeasy Plus Micro kit, Qiagen) and cDNA constructs were inserted in TOPO II PCR-plasmids using TOPO TA Cloning kit with chemically competent cells according to manufacturer’s protocol (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA was extracted from mouse testis using the RNeasy extraction kit (74104, Qiagen, Hilden Germany) according to the manufacturer’s instruction ...
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bioRxiv - Systems Biology 2023Quote: ... RNAs were extracted from isolated mouse SAN with RNeasy Mini Kit (Qiagen, Valencia, CA, United States) and DNAse ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was isolated from mouse tail snips using DNeasy® Blood & Tissue Kit (Qiagen, Valencia, CA) and genotyped ...
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bioRxiv - Immunology 2023Quote: The RNA extraction process involved lysing mouse colonic IEC or cultured cells using RLT buffer (QIAGEN) and extracting total RNA using the RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Physiology 2024Quote: Total RNA from mouse and human islets was extracted using a RNeasy micro kit (Qiagen # 74004) and reverse transcribed using a SuperScript™ VILO™ Master Mix (Invitrogen # 11755050) ...
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bioRxiv - Cell Biology 2024Quote: DNA and RNA samples were isolated from mouse islets with the Blood/Tissue DNeasy kit (Qiagen) and MicroElute total RNA kit ...
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bioRxiv - Genomics 2024Quote: We extracted genomic DNA from tail of each mouse using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from prefrontal cortex of 8-month-old mouse using RNeasy kit (Qiagen). RNA amount was quantified by ‘Nanodrop’ spectrophotometer and integrity was assessed by TapeStation (Agilent) ...
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bioRxiv - Evolutionary Biology 2021Quote: Protein sequence alignments and tree production were made using the CLC main work bench (ver. 5.5.1, Qiagen) software package ...
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bioRxiv - Physiology 2019Quote: ... RNA was extracted using the AllPrep DNA/RNA/Protein Mini Kit according to manufacturer’s instructions (Qiagen, 80004). RNA quality was determined by Agilent 2200 TapeStation and quantified by Qubit (Life Technologies) ...
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bioRxiv - Plant Biology 2019Quote: Sitting-drop vapour-diffusion experiments identified suitable crystallisation conditions for native protein from the PACT screen (QIAGEN), with optimisation identifying the best conditions for crystal formation as 6 mg/mL protein combined 1:1 with 8% PEG 1500 ...
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bioRxiv - Microbiology 2019Quote: ... The DNA from the appendix samples was obtained using the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN), before the DNA extraction ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... and His-tagged protein was purified from cell lysate using a Ni-NTA spin column (Qiagen, 31014). The resulting product was run on a 12% Bis-Tris PAGE gel (Thermofisher ...
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bioRxiv - Molecular Biology 2020Quote: Nme2Cas9 protein was expressed in Rosetta (DE3) cells and purified using a Ni2+-NTA agarose column (QIAGEN) as previously described24 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 mM TCEP) the expressed protein was extracted from the supernatant using NiNTA resin (QIAGEN, Germantown, MD). The resulting eluate from the NiNTA resin using lysis buffer supplemented with 250 mM Imidazole pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... 6xHis tagged protein were purified by immobilized metal ion chromatography using nickel-NTA super flow resin (Qiagen) as previously described with slight modifications46 ...
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bioRxiv - Plant Biology 2021Quote: Alignments of histone DNA and protein sequences was performed using CLC Main Workbench software version 8.1 (QIAGEN). Predictions of intrinsic disorder were undertaken by PONDR (58) ...
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bioRxiv - Immunology 2022Quote: ... https://www.beiresources.org/).Protein was purified using gravity flow purification with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen, Germany) and concentrated and buffer exchanged in Amicon centrifugal units (EMD Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... Expressed protein was purified by elution from a nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Germantown, MD) column with 0.5 M imidazole and buffer exchanged into TEV buffer (25 mM NaHPO4 ...
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bioRxiv - Genomics 2021Quote: ... RNA-protein complexes were then incubated with Ni-NTA sepharose (Qiagen, 100 μl of slurry per sample) overnight at 4°C and extensively washed ...
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bioRxiv - Developmental Biology 2020Quote: ... The significantly changed proteins were used as inputs for Ingenuity Pathway Analysis (IPA, Version 03-13, Qiagen). The IPA predicted the altered pathways ...
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bioRxiv - Biochemistry 2021Quote: ... All the protein samples were expressed in BL21(DE3) and purified using Ni-NTA Sepharose column (QIAGEN) and Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The polyhistidine-tagged recombinant proteins were then purified from bacterial lysates with Ni2+-NTA agarose beads (QIAGEN) and washed by three different washing buffers (Washing buffer 1 contained 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA were isolated from equivalent amounts of HDL total protein using the miRNeasy Mini Kit (Qiagen). For sRNA analyses ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... 0.2 µg of AFP1-His proteins was incubated with 20 µl Ni-NTA agarose beads (Qiagen, Germany) in buffer (25 mM Tris-Cl ...
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bioRxiv - Immunology 2020Quote: ... HIS-tagged DR protein monomers were produced by Hi5 insect cells and purified using Ni-NTA (Qiagen) and size exclusion columns (AKTA ...
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bioRxiv - Microbiology 2022Quote: ... and RNA was isolated using the All prep bacterial DNA/RNA/protein kit following the manufacturer’s (Qiagen) protocol ...
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bioRxiv - Microbiology 2022Quote: ... The biomolecular extraction was then performed using the commercial AllPrep DNA/RNA/Protein Mini Kit (Qiagen-80004), conducted on a customised robotic system owned by the lab (Tecan-LU_UNILU_EWS_EXTRACTION_EU-0908-Freedom EVO 200) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen, Germantown, Maryland). Detection of proteins via Western blotting was performed by fluorescence detection using IR-Dye®-labeled fluorescent secondary antibodies and imaged using the Odyssey CLx Imager (LICOR Biosciences ...
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bioRxiv - Plant Biology 2023Quote: ... The His-ZmTPL2N-His recombinant protein was purified using Pierce Ni-NTA resin (QIAGEN, Cat. No. 30210) according to the product protocol ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Immunology 2023Quote: ... Protein exaction from the deparaffinised sample was carried out using the Qiagen Qproteome kit (Qiagen, Hilden, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from all the cell lines using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). The total RNA was then treated with Turbo DNA-free kit (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: Melting curves of proteins were measured using the Rotor-Gene Q real-time PCR cycler (Qiagen, Germany). 25 μl samples with a protein concentration of approximately 0.5 mg/ml in a buffer containing 100 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA for each Klebsiella strain was extracted using the AllPrep Bacterial DNA/RNA/Protein kit (QIAGEN) according to the manufacturer’s instructions ...