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Citations for Qiagen :
51 - 100 of 216 citations for Nickel oxalate dihydrate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... lysed and incubated with nickel-nitrilotriacetic acid beads (Qiagen, Hilden, Germany) as per the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cleared lysates were incubated with 2-4ml nickel NTA beads (Qiagen) for 20-40 minutes before washing beads with 5-10 column volumes of lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was incubated with nickel affinity resins (Ni-NTA, Qiagen) at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and rEDA was purified using nickel-nitrilotriacetic acid-agarose resin (Qiagen).
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bioRxiv - Biophysics 2024Quote: ... Pre-equilibrated nickel-NTA resin (Qiagen, 50 mg/mL binding capacity) was added to the clarified lysate and the lysate-resin slurry stirred in a beaker with a stir bar for 1 hour at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed with 20 ml nickel affinity resin (Qiagen) and rotated at 4 °C overnight.
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bioRxiv - Molecular Biology 2023Quote: ... using nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (QIAGEN, cat # 30230) and the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... HAs were purified from the supernatant using nickel-NTA agarose (Qiagen) and disposable 10mL polypropylene columns (Thermofisher) ...
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bioRxiv - Cell Biology 2020Quote: ... the complex was purified by nickel affinity chromatography (Ni-NTA resin, Qiagen) and eluted with elution buffer (40mM Tris pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Protein purification was undertaken using nickel-affinity chromatography (Qiagen, Valencia, CA, USA) and endotoxin was removed by two-phase extraction with Triton X-114 22 ...
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bioRxiv - Cell Biology 2020Quote: ... the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose beads (QIAGEN) pre-equilibrated with the washing buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... and proteins were purified by two successive nickel affinity chromatography (Qiagen®) steps ...
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bioRxiv - Molecular Biology 2022Quote: ... the His-SUMO conjugates were enriched using nickel-nitrilotriacetic acid beads (Qiagen). Upon multiple washing steps ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 µL of dry nickel-nitrilotriacetic acid-agarose (Ni-NTA) beads (QIAGEN), were equilibrated with Guanidinium buffer supplemented with 5 mM β-mercaptoethanol and 50 mM Imidazole pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by purification using nickel-nitrilotriacetic acid (Ni-NTA) agarose resin (QIAGEN). The recombinant protein was desalted with PD-10 column (SephadexTM G-25M ...
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bioRxiv - Bioengineering 2021Quote: ... His-tagged proteins were purified using a nickel column purification step (QIAGEN). Following IMAC ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified by Nickel affinity chromatography (Ni2+-NTA-sepharose resin, Qiagen). His6-FUBI-eS30 WT and AA constructs were expressed in E ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was purified using a nickel gravity column (Qiagen, Ni-NTA Agarose) followed by a high-salt wash (1xPBS ...
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bioRxiv - Molecular Biology 2020Quote: ... added to 100 μl washed Ni-NTA agarose nickel beads slurry (Qiagen), and transferred to Snap Cap spin columns (Pierce).
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bioRxiv - Microbiology 2021Quote: ... the supernatant was extracted with a nickel-nitrilotriacetic acid-agarose suspension (Qiagen) or a Pierce GST Spin Purification Kit (Thermo ...
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bioRxiv - Neuroscience 2023Quote: ... His-tagged VndA was purified over a Nickel-NTA agarose resin (Qiagen) according to manufacturer’s recommendation ...
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bioRxiv - Biophysics 2023Quote: ... GlpG in supernatant was purified using nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) affinity chromatography in 50 mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA). Stain free gels (4-12% ...
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bioRxiv - Biochemistry 2023Quote: ... for GST-tagged proteins or nickel– nitriloacetic acid (Ni-NTA) agarose (Qiagen) for His-tagged proteins (2 hr ...
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bioRxiv - Microbiology 2024Quote: ... The protein was purified from the supernatant via nickel affinity chromatography (Qiagen). The protein was further purified by a Hiload 16/60 Superdex 200 column (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μL of dry nickel-nitrilotriacetic acid-agarose (Ni-NTA) beads (QIAGEN), were equilibrated with Guanidinium buffer supplemented with 5 mM β-mercaptoethanol and 50 mM Imidazole pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were purified over nickel-nitrilotriacetic acid (Ni-NTA) Agarose (Qiagen). Therefore ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified supernatant was incubated with nickel-nitrilotriacetic acid agarose resin (QIAGEN) for 3 hours at room temperature ...
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bioRxiv - Genomics 2024Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA), and stain-free gels (4-12% ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was incubated with Ni-NTA agarose nickel-charged resins (Qiagen) that had been equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining lysates were incubated with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was mixed with nickel-nitrilotriacetic acid agarose beads (Qiagen, Hilden, Germany) and loaded on a column ...
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bioRxiv - Biochemistry 2020Quote: ... The target proteins were purified with nickel chelate affinity resins (Ni-NTA, Qiagen). The protein samples were collected and purified by a Superdex 200 (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) and elution with imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Soluble ACE 2 was purified using Nickel -NTA agarose beads (Qiagen Cat.No-1018244), eluted with 200mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... and the soluble fractions were purified using nickel affinity chromatography (Qiagen, Manchester, UK) and eluted with lysis buffer containing 300 mM imidazole ...
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bioRxiv - Cancer Biology 2023Quote: ... all proteins were purified using Ni-NTA (nickel-nitrilotriacetic acid) resin from QIAGEN. After the incubation of Expi293 media supernatant with the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were first purified using nickel-nitrilotriacetic acid (NTA) agarose resin (Qiagen), and the His8-SUMO tag was then removed by TEV protease (10:1 w/w ...
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bioRxiv - Cancer Biology 2022Quote: ... and the supernatants were purified using a nickel-NTA affinity column (Qiagen 30210). Centrifugal filters with molecular 10 kDa molecular weight cutoffs (Millipore UFC801024 ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Nickel sepharose according to the manufacturer’s instructions (QIAGEN). The purified recombinant protein was dialyzed overnight against dialysis buffer (20 mM Tris-HCl ...
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bioRxiv - Bioengineering 2024Quote: ... AbLecs were purified by manual gravity column using nickel-NTA agarose resin (Qiagen). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... Affinity purification was performed using nickel nitriloacetic acid (Ni-NTA) affinity column (Qiagen) attached to an AKTA™ start system (Cytiva) ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatant was loaded on a nickel nitrilotriacetic acid (Ni-NTA) resin (Qiagen GmbH) column ...
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bioRxiv - Cell Biology 2021Quote: ... We purified the 6xHIS fusion protein on nickel-nitrilotriacetic acid agarose (Qiagen, Valencia, CA) according to the manufacturer’s protocols ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: ... and nickel-nitrilotriacetic acid (Ni-NTA) agarose resin was purchased from Qiagen (Hilden, Germany). PCR reagents ...
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bioRxiv - Biophysics 2020Quote: ... The enzyme was purified through a nickel-nitrilotriacetic acid column (Ni-NTA superflow, Qiagen), which was followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Cell Biology 2020Quote: ... were purified by two rounds of nickel affinity chromatography using Ni-NTA agarose (QIAGEN), with overnight cleavage by N-terminally His-tagged TEV protease (expressed in our laboratory ...
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bioRxiv - Cell Biology 2020Quote: ... Soluble material was then were added to NTA nickel resin (400 µL slurry, Qiagen), which was pre-charged (overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM 2-mercaptoethanol) and the proteins eluted from nickel-NTA agarose beads (Qiagen; using buffer containing 25 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant HisCheY or HisCheY* and bound proteins were purified using nickel agarose columns (Qiagen) under native conditions per manufacturers’ instructions.