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Citations for Qiagen :
51 - 100 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the mixture was incubated with Glutathione Sepharose™ 4B (Cytiva) and Ni-NTA Superflow (Qiagen). The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from each plate using RNeasy mini kit (QIAgen) and QIAshredder following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and final glucose plates using the DNeasy Blood & Tissue Kit (QIAGEN). We then amplified the chimeric acrIIA4 locus from the genomic DNA ...
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bioRxiv - Molecular Biology 2019Quote: ... His6- and GST-tagged proteins were affinity-purified in batch on Ni-NTA agarose (Qiagen) and glutathione Sepharose 4 Fast Flow (GE Healthcare) ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from cells (1-2 wells, 6 well plate) using an RNeasy Plus Mini Kit (Qiagen, Hilden, Germany), including a DNase step to remove residual DNA ...
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Genomic approach for conservation and the sustainable management of endangered species of the AmazonbioRxiv - Genomics 2020Quote: ... using 5.0 μL 2X Qiagen Multiplex PCR Master Mix (Qiagen),S 1.0 μL of Q-Solution (Qiagen), 2.0 μL of H2O ...
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bioRxiv - Immunology 2024Quote: ... 25mg of tissue was dissociated using Bead Ruptor Elite (Omni International) for 45 seconds at 4.85m/s and RNA was isolated using RNeasy Fibrous Tissue Mini Kit 50 (Qiagen) following manufacturer’s indications ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of a 10 min bead beating step at 50 s−1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure-Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... and homogenized at 25/s for 1 min at 4°C using a TissueLyser II (Qiagen, 85300). Samples were incubated at room temperature for 5 min followed by addition of 180 μl chloroform ...
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bioRxiv - Molecular Biology 2019Quote: ... The desired ~3 kb product was separated on a 1x TBE agarose gel and recovered by electroelution and ethanol precipitation (fluorescent DNA substrates) or with the QIAquick gel extraction kit (Qiagen).
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bioRxiv - Systems Biology 2023Quote: ... RNA was extracted using the RNeasy 96-well plate kit (Qiagen, #74181) according to manufacturer’s instructions including the DNase step ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
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bioRxiv - Cell Biology 2019Quote: ... The mixture was washed 20x with 20 ml GST washing buffer on a disposable column (QIAGEN) The GST-fused protein was eluted with 1 ml GST elution buffer and the eluted sample was dialyzed overnight at 4°C in 2 l 1x PBS in a Slide-A-Lyzer Dialysis Cassette G2 10 000 MWCO (Thermo Scientific).
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bioRxiv - Bioengineering 2023Quote: ... total RNA was isolated from Hep3B cell spheroids on the pillar plate and in the ULA plate using a RNeasy Plus kit (Qiagen,74134). One μg of RNA was converted into cDNA using the high-capacity cDNA reverse transcription kit according to the manufacturer instructions (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: Cytokine- or vehicle-stimulated primary CD1 astrocyte cultures in 6-well plates and neuron cultures in 24-well plates were lysed and their total RNA extracted using RNeasy Mini Kits (Qiagen 74104) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... DNA was isolated from thawed APOE cell lines by Bioneer A/S using the Blood & Cell Culture DNA Mini Kit (Qiagen). For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... at 6 m/s for 50 sec and total RNA extracted with the RNeasy Lipid Tissue Mini Kit (#74804; Qiagen). For SCP (D18 ...
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FGF signalling is involved in cumulus migration in the common house spider Parasteatoda tepidariorumbioRxiv - Developmental Biology 2021Quote: CLC Main Workbench 7 (QIAGEN Aarhus A/S) was used to perform a local tBLASTn [25] against the Parasteatoda tepidariorum official AUGUSTUS gene set (https://i5k.nal.usda.gov/Parasteatoda_tepidariorum ...
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bioRxiv - Microbiology 2020Quote: ... version 10.0 (Qiagen Aarhus A/S, Aarhus, Denmark), and a Basic Variant Detection 2.0 analysis was performed ...
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bioRxiv - Cell Biology 2022Quote: ... Condensin I was bound to Glutathione Sepharose 4B beads (Cytiva) in a 5 mL polypropylene column (Qiagen) using GST- tagged mSMC4 and eluted by PreScission Protease (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was incubated under gentle shaking overnight with glutathione matrix (Qiagen; 1ml per 1000ml cell culture) at 4°C ...
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bioRxiv - Genetics 2023Quote: RNA was extracted from 6-well plates using miRNeasy Mini Kits (QIAGEN 217004) with the inclusion of an on-column DNase digestion (QIAGEN 79254) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted directly from the plates using RNeasy Mini Kit (Qiagen, 74104). 2μg of RNA were used for cDNA synthesis using High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 viriants S and point-mutated pseudovirus were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Cat#52906), and served as template for reverse transcription using the TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR reagent (TransGen Biotech ...
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bioRxiv - Plant Biology 2022Quote: Two RNA samples from rosette leaves of after-bolting WT plants and bglu28/30-2 mutant plant grown under different S conditions and their 7-day-post-anthesis siliques were extracted with the RNeasy Plant Mini Kit (QIAGEN, Germany). The residual DNA was removed with DNase treatment (DNaseI ...
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bioRxiv - Microbiology 2019Quote: ... and then amplified using 40 cycles of 95°C for 3 s and 60°C for 30 s using either the Rotor-Gene Q or Rotor-Gene 6000 platform (Qiagen, Hilden, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Cleaved CK1δ ΔC was further purified away from His-GST and His-TEV using Ni-NTA resin (Qiagen) and subsequent size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Systems Biology 2019Quote: ... Plates were prepared using the miRCURY SYBR Green PCR Kit (Qiagen, cat. no. 339346) with custom miRCURY LNA PCR primers (Qiagen cat ...
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bioRxiv - Immunology 2024Quote: ... purified RNA is extracted using the RNeasy 96 well plate isolation kit (Qiagen #74182).
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA from representative isolate(s) for each study participant was extracted using a QIAGEN DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany) and the 16S rRNA gene was amplified using the primers 63f and 1387r.53 Amplicons were purified enzymatically with ExoSAP-It (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: The DNA of all 52 samples was extracted from silica-gel dried leaves using the Qiagen DNeasy Plant Mini Kit following slightly modified manufacturer′s instructions (Qiagen, Valencia, CA). DNA quality and concentration were verified and quantified using NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: A region-specific polymerase chain reaction (PCR) was performed for the S-COMT promoter region using the PyroMark PCR Kit (Qiagen, Hilden, GER) according to manufacturer’s instructions with previously published primers (F ...
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bioRxiv - Microbiology 2021Quote: ... and a DeNovix® dsDNA Broad Range Assay Kit following the manufacturer’s instructions.Total genomic DNA was isolated from a subset of test diets using the DNeasy PowerMax® Soil kit (Qiagen, Hilden, Germany) to assess our ability to identify C ...
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bioRxiv - Cell Biology 2024Quote: ... tissue was snap frozen in liquid nitrogen and pulverized with a microdismembrator S (B. Braun Biotech, Melsungen, Germany) before total RNA isolation by means of the Lipid Tissue Mini Kit (Qiagen, Hilden, Germany). Isolated cells were lysed in RLT buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... along with 8 prickleless parental accession individuals (S. macrocarpon PI 441914) was extracted from young leaf tissue using a DNeasy Plant Pro Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions for high-polysaccharide content plant tissue ...
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bioRxiv - Cell Biology 2023Quote: ... Complexes were pulled down using Glutathione-Sepharose 4B resin (Cytiva; 17-0756-01) or Ni-NTA resin (QIAGEN; 30210) for 2 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The soluble lysates obtained after two rounds of centrifugation at 18,000g and 4°C were incubated with glutathione agarose beads (Pierce) or Ni2+-NTA beads (QIAGEN). Recombinant proteins were eluted from the beads using 10 mM reduced glutathione in a Tris (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... The His and GST tag were cleaved by the Tev protease and separated from AtPAXXL228E using Ni-NTA (Qiagen). AtPAXXL228E was loaded onto a HiTrap Q HP column equilibrated with 20 mM Tris pH7.4 ...
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bioRxiv - Microbiology 2022Quote: ... 100μL of viral supernatant was collected in S-Block (Qiagen) previously loaded with VXL lysis buffer containing proteinase K and RNA carrier ...
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bioRxiv - Physiology 2021Quote: ... by shaking for 50 s at 30 Hz (TissueLyser, Qiagen). 200 μL chloroform was added to homogenize Trizol samples ...
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bioRxiv - Microbiology 2021Quote: ... 100µL of viral supernatant was collected in S-Block (Qiagen) previously loaded with VXL lysis buffer containing proteinase K and RNA carrier ...
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bioRxiv - Microbiology 2020Quote: ... 100µL of viral supernatant was collected in S-Block (Qiagen) previously loaded with VXL lysis buffer containing proteinase K and RNA carrier ...
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bioRxiv - Microbiology 2022Quote: ... using CLC Genomics Grid Worker v11.0.3 (Qiagen Aarhus A/S) with default parameters.
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bioRxiv - Genomics 2019Quote: ... Plates grown in parallel were used for genomic DNA extraction (DNeasy Blood & Tissue kit, Qiagen), and RNA extraction (TRIzol ...
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bioRxiv - Microbiology 2021Quote: ... These cells were incubated overnight in MEM-α media free of FBS and supplemented with 1% P/S followed by collection of Supernatant (conditioned media) and total RNA extraction (Cat# 74134, Qiagen, Germany). The resulting samples were stored at −80°C until analyzed further in downstream applications.
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bioRxiv - Cancer Biology 2019Quote: ... Germany) using a metal ball for 2×2 min at 25 s−1 in 600 μl of RLT buffer (Qiagen, Germany) with 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and homogenized at a frequency of 26/s for 1 minute in a TissueLyser II (Qiagen, Germantown, MD) and centrifuged at 16,500xg for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: The GST fusion proteins were expressed in bacteria (E. coli, BL21) and purified by glutathione-Sepharose (Cat No. 27-4574-01, Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was added to a 500 μl equilibrated Glutathione Sepharose 4 Fast Flow affinity medium (GE) or Ni-NTA agarose (Qiagen). The lysate was incubated with an affinity matrix for 1 hour at 4 ⁰C ...
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bioRxiv - Microbiology 2019Quote: ... Cignal EGR-1 reporter kit (Qiagen) was transfected in HEK293T following manufacturer protocol ...