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Citations for Qiagen :
801 - 850 of 2178 citations for 7 Oxa 3 azabicyclo 4.1.0 heptane 1 methyl 3 propyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... the cells were washed once with 0.1% PBSA and incubated with 50 μL of a 1:100 dilution of anti-penta-His Alexa 647 antibody (Qiagen) for 10 minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... or M-MØ (1×106 cells/well) were transfected with 1 μg of the XRE reporter construct using either Superfect (Qiagen) or VIROMER RED (Lipocalix) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with a 1:1 molar ratio of eGFP-P2A-Cas9 and single stranded oligonucleotide using Effectine (Qiagen) in a six-well dish ...
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bioRxiv - Biochemistry 2020Quote: ... The restriction digestion efficiency was validated using 1% 1× TBE agarose gel electrophoresis and purified with a QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... homogenized in 1 ml PBS by bead beating (3mm steel ball, 25 Hz for 1 minute in a TissueLyser (Qiagen)) ...
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bioRxiv - Genomics 2021Quote: ... 1 g of ground leaf powder was resuspended in lysis buffer with 1 mg/ml of proteinase K (19133, Qiagen) and 190 μg/ml of RNase A (19101 ...
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from 1×106 MCF7 and 1×106 BT-474 cells (‘bulk’) using the RNeasy Mini Kit (74104, Qiagen) in combination with the QIAshredder (79654 ...
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bioRxiv - Immunology 2022Quote: ... For bacterial load determination one lobe of the lung was collected in 1 mL PBS with 1 metal bead and smashed using a tissue lyser (Qiagen). 25 μL of lysed lung were put in duplicates on CYE-plates and grown for 3-4 days at 37°C in a non-CO2 incubator.
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bioRxiv - Physiology 2024Quote: ... After chloroform incubation and centrifugation per manufacture instructions RNA-containing phase was diluted with 70% ethanol 1:1 and RNA isolation was performed using RNeasy Mini Kit (Qiagen). For tissues ...
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bioRxiv - Microbiology 2023Quote: ... was added to the supernatant to give a final concentration of 1 mM and the resultant suspension was applied to a 1 ml Ni2+-NTA column (Qiagen). The resin was then washed sequentially with 10 ml of lysis buffer (without Triton X-100) ...
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bioRxiv - Cell Biology 2023Quote: ... The aqueous phase was mixed 1:1 with 70% ethanol and applied to a minikit spin column and washed with RW1 and RPE buffer (Qiagen). cDNA was synthesized using the QuantiTect Rev ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
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bioRxiv - Plant Biology 2023Quote: ... of g1090::XVE>>AXR3-1-mCherry and HS::AXR3-1 were extracted following the protocol (Plant Total RNA Mini Kit, QIAGEN). g1090::XVE>>AXR3-1-mCherry were transferred for 2 h on estradiol-containing medium and then harvested ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 1:1 of 70% ethanol was mixed with the upper phase before transfer on column (RNeasy Mini kit, Qiagen), and RNA was extracted according to manufacturer directions ...
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bioRxiv - Bioengineering 2022Quote: ... the aqueous phase was mixed with ethanol in a 1:1 ratio and was added to RNeasy spin columns (Qiagen) for RNA purification ...
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bioRxiv - Cancer Biology 2023Quote: ... Digested PCR fragments were ligated into the restricted vector at a 1:1 molar ratio using T4 DNA ligase and were purified using the MiniElute PCR purification kit (Qiagen). Purified ligated plasmids were electroporated into MegaX DH10B (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... The significantly differentially expressed genes with a log2 fold change <= −1 or >= 1 were input into Ingenuity Pathway Analysis (Qiagen) to determine pathway enrichment (significance determined by Fischer’s exact method).
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bioRxiv - Molecular Biology 2024Quote: ... fungal DNA was extracted independently from 1 mL of crude conidial suspensions (between 108 and 109 spores mL-1) using the DNeasy 96 Plant Kit (Qiagen) following the manufacturer’s instructions (Gautier et al. ...
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bioRxiv - Immunology 2021Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen) ...
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bioRxiv - Genomics 2020Quote: ... and 0.5 mg.ml-1 of Proteinase K (Qiagen), to reverse formaldehyde crosslinking ...
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bioRxiv - Microbiology 2019Quote: ... 1 ml RLT buffer (Qiagen RNA Isolation Kit) + 10 μl β-mercaptoethanol was added and vortexed and a phenol chloroform extraction followed by an ethanol precipitation was carried out ...
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bioRxiv - Genomics 2020Quote: ... cells were mixed 1:2 with RNAProtect (Qiagen) and harvested via centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... and 1-5mL Qiazol Lysis Reagent (Qiagen, #79306) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... amplification grade (1 U/µg RNA, Qiagen, #79254). Real-time qPCR was performed with a MyiQ™ instrument (BIO-RAD) ...
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bioRxiv - Biochemistry 2022Quote: ... Penta-His Antibody (Qiagen, 34660, IB: 1:1000) and Y188 to APP C-terminus (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... with on-column DNase-1 digestion (79254, Qiagen). Purified RNA had 260/280 ratios ~2 (via Nanodrop 2000c ...
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bioRxiv - Neuroscience 2019Quote: ... 5nmol of Rho-1 siRNA (Qiagen, Germany #SI01401743) was diluted in rnase-free water (provided in kit ...
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bioRxiv - Biochemistry 2021Quote: ... passed over 1 mL Ni-NTA resin (Qiagen), and bound protein eluted with 10 CV of buffer A / 200 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Genetics 2019Quote: ... 1× Type-it Multiplex PCR Master Mix (Qiagen), 0.2 μM of each locus specific reverse primers ...
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bioRxiv - Genetics 2020Quote: ... 1× Multiplex PCR Master Mix (Qiagen, Hilden, Germany), and 0.2 μM of Primer_F3 and Primer_R (Figure S2 and Table S2) ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen):cDNA) ...
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bioRxiv - Genomics 2022Quote: ... 1 mL of buffer PB or PM (Qiagen), containing a high concentration of guanidine hydrochloride and isopropanol was added and mixed by pipetting ...
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bioRxiv - Physiology 2022Quote: ... Samples were homogenized in 1 ml Qiazol (Qiagen) with 5 μl β-mercaptoethanol using a beadmill homogenizer cooled with liquid nitrogen and then phase separated using 1-bromo-3-chloropropane (BCP) ...
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bioRxiv - Biochemistry 2024Quote: ... and anti-Strep (Qiagen, 34850, 1:1000 dilution) antibodies ...
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bioRxiv - Immunology 2023Quote: ... Samples were diluted 4:1 (elution buffer (Qiagen): cDNA ...
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bioRxiv - Biophysics 2023Quote: ... Approximately 1 ml of Ni-NTA agarose (Qiagen) was utilised for purification of the lysate resulting from 1 l culture ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mL of RNA protect reagent (76506, Qiagen) was added to 500 µL of the sample ...
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bioRxiv - Systems Biology 2023Quote: ... 1 mL of ice-cold Qiazol (Qiagen, 79306) reagent was added and transferred into Precellys® lysing kit tubes (Keramik-kit 1.4/2.8 mm ...
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bioRxiv - Biochemistry 2024Quote: ... and 1% Protease Inhibitor using TissueLyser II (Qiagen) and 5 mm stainless steel beads (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... Proteinase K (20 μg·ml-1 final concentration, Qiagen) was firstly added to the relevant samples ...
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bioRxiv - Physiology 2024Quote: ... and QuantiTect primer assays (QIAGEN; Suppl. Table 1). Expression was determined in triplicate from each biological replicate ...
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bioRxiv - Immunology 2024Quote: ... Samples were diluted 9:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... FAF1 (Uniprot identifier Q9UNN5-1) and UBXN7 (Uniprot identifier O94888-1) were amplified from XpressRef Universal Total human RNA (QIAGEN, 338112) by RT-PCR (TaKaRa ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... and were subjected to three cycles of grinding (1 minute, at 30 Hz followed by 1 minute without agitation) in a TissueLyser II apparatus (Qiagen, France). The solutions obtained from crushed nematodes or the contents of G ...
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bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted with 1 volume of 25:24:1 phenol-chloroform-isoamyl alcohol and purified with QIAquick PCR Purification Kit (QIAGEN 28104). Libraries were prepared with the Ovation Ultralow V2 DNA-Seq Library Preparation Kit (NuGEN (Redwood City ...
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bioRxiv - Immunology 2023Quote: ... 120 mg l− 1 sodium pyruvate and 1% penicillin–streptomycin) at 300 Hz for 2 minutes using a TissueLyser II (Qiagen, Germany).
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated for 1 hour with monoclonal α-His antibodies conjugated to the horseradish peroxidase (1:5000 dilution; Qiagen). Blots were visualized with the addition of Lumina Forte Western HRP Substrate (MilliporeSigma ...