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Citations for Qiagen :
751 - 800 of 6187 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: SARS-CoV-2 RNA copy number was quantified in RNA samples extracted from infected cells using the QuantiTect probe RT-PCR Kit (Qiagen). TWe prepared a standard curve using the ORF9 nucleoprotein coding sequence cloned in pcDNA3 ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then diluted 25 times to serve as template for real-time quantitative PCR (RT-qPCR) using Rotor-Gene 6000 (Corbett Research, QIAgen), as previously described (Forlenza et al. ...
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bioRxiv - Microbiology 2021Quote: ... 10 µl of the cell suspension from each dilution was dispensed into 24 wells of a 96 well plate containing 2 μl One-step RT-PCR enzyme (Qiagen), 10 μl 5x One-step RT-PCR buffer (Qiagen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were done with the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
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bioRxiv - Microbiology 2020Quote: The 16 h clindamycin-induced and uninduced seed cultures were cultivated and incubated in 1 ml of RNA Protect in the same manner as for RT-PCR Total RNA was extracted with an RNeasy RNA isolation kit (Qiagen). The isolated RNA was treated with DNaseI (0,1 U μl-1 ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR assay (Corman et al., 2020; Wolfel et al., 2020) using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... The SVIP-MPv2 assay was developed and validated as a one-step procedure using the QuantiNova Probe RT-PCR Kit (Qiagen) with final concentrations of primers (1.4μM each ...
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bioRxiv - Bioengineering 2021Quote: ... Total RNA (5 μl) was used for cDNA synthesis and qPCR was performed in one step using QuantiTect Probe RT-PCR (Qiagen) on a StepOnePlus System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... Gene expression was evaluated by qPCR using the ONE-step QuantiTect Probe RT-PCR Kit (Qiagen, Cat No./ID: 204445). For each reaction 2 μL of RNA were used ...
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bioRxiv - Developmental Biology 2020Quote: ... and 10ng of RNA per sample was used in each reaction according to instruction in QuantiFast SYBR Green RT-PCR kit (QIAGEN). Samples were subsequently loaded on to a LightCycler 480 Instrument II (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl RNA was used in a one-step real-time RT-PCR E assay33 using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Pathology 2021Quote: ... and gene expression was carried using the RT² Profiler PCR Array for human cellular stress responses (Qiagen, Catalog#:PAHS-019ZA) following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... reverse transcription and amplification of the S gene were performed using the one-step QuantiFast Sybr Green RT-PCR mix (Qiagen) as follows ...
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bioRxiv - Pathology 2021Quote: ... were premixed and added to each well of RT² Profiler™ PCR Array Mouse NFκB Signaling Pathway (Qiagen, PAMM-025Z) for target gene qPCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... 5 μl RNA was used in a one-step real-time RT–PCR E assay using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... Five μl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR was performed as previously described9 with miRCURY LNA RT and SYBR Green PCR miRNA kits and miRCURY LNA probes for miRNAs (Qiagen) and High-capacity cDNA transcription kit and Fast SYBR Green (Qiagen ...
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bioRxiv - Immunology 2021Quote: Cytokines and cellular sensors were measured in neonatal lung homogenates (cDNA prepared as per viral titres) by RT-qPCR using QuantiFast SYBR Green PCR Kit (Qiagen) and QuantiTect Primer Assays (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed with a primer set targeting partial regions of the ORF1b gene in the SARS-CoV-2 virus using the QIAGEN OneStep RT-PCR kit (Qiagen) as previously reported (51) ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
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bioRxiv - Bioengineering 2023Quote: Reverse transcription of isolated miRNAs for quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed with a miScript II RT Kit (Qiagen) for selected miRNA targets ...
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bioRxiv - Microbiology 2023Quote: A 388 bp region surrounding NS4A position 19 was amplified from total RNA extracted from a subset of brains using OneStep RT-PCR (Qiagen) using the following primers ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 ng of total RNA was used for the retrotranscription (RT) reaction using the miRCURY LNA miRNA PCR system (Qiagen). After dilution of the resulting cDNA ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... sequence: TCAGTCTGATAAGCT) and its corresponding microRNA-21 control (miRCURY LNA™ microRNA-21a-5p control; sequence: TCAGTATTAGCAGCT) were purchased from Qiagen (Hilden, Germany), resuspended in PBS and injected at a dose of 10 mg/kg (s.c. ...
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bioRxiv - Cancer Biology 2021Quote: ... DNase (RNase-Free DNase Set, Qiagen, catalog#:79254) was used to degrade DNA during the extraction as outlined in the RNeasy Mini Handbook ...
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bioRxiv - Genetics 2021Quote: ... with RNase-Free DNase Set (Qiagen no. 79254) according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and the RNase-Free DNase Set (#79254; Qiagen). The isolated heart RNA was converted into cDNA by QuantiTect Reverse Transcription Kit (#205311 ...
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bioRxiv - Immunology 2022Quote: ... treated with DNase (RNase-Free DNase Set, Qiagen), and reverse transcribed to generate cDNA with High-Capacity RNA-to-cDNA™ Kit (Themo-Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... RNase-Free DNase Set (Qiagen, cat no. 79254) was used for further DNase digestion ...
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bioRxiv - Plant Biology 2019Quote: ... and treated with RNase-Free DNase Set (QIAGEN). Reverse transcription was carried out using the SuperScript IV Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Neuroscience 2019Quote: ... or with RNase-Free DNase Set (Qiagen, #79254) (larval OSN nuclei samples) ...
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bioRxiv - Microbiology 2020Quote: ... was treated with RNase-Free DNase Set (Qiagen) according to the manufacturer’s protocol to remove possible traces of genomic DNA.
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bioRxiv - Genetics 2021Quote: ... and treated with RNase-free DNase Set (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Following DNase treatment (RNAseq-Free DNAse Set (Qiagen)) and RNA purification (RNeasy MinElute Kit) ...
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bioRxiv - Plant Biology 2021Quote: ... along with the RNase-free DNase Set (Qiagen). We constructed sequencing libraries with the KAPA Stranded mRNA-seq Kit (KAPA Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... and the DNAse RNase-Free Set (Qiagen, Netherlands) according to manufacturer’s instructions and as described previously (12) ...
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bioRxiv - Plant Biology 2021Quote: ... and treated with RNase-Free DNase Set (QIAGEN). Reverse transcription was carried out using the SuperScript IV Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Genetics 2020Quote: ... in conjunction with RNase-Free DNase Set (Qiagen). cDNA was generated using the High-Capacity RNA-to-cDNA™ Kit (Applied Biosystems).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... combined with the RNase-Free DNase Set (Qiagen). We stored RNA at -70°C ...
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bioRxiv - Cell Biology 2022Quote: ... DNAwas removed (RNase-Free DNase Set; Qiagen, Japan) and RNA recovered by column purification (QIAquick PCR Purification Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... QIAshredder Columns and RNase-Free DNase Set (Qiagen). RNA-Seq library construction and sequencing were performed according to protocols used by the integrated genomics operation (IGO ...
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bioRxiv - Cancer Biology 2022Quote: ... QIAshredder Columns and RNase-Free DNase Set (Qiagen). cDNA synthesis was performed using TaqMan® Reverse Transcription Reagents (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... with DNaseI treatment (RNase free DNase Set, Qiagen). RNA quality was confirmed by RNA Tapestration and quantified with Qbit ...
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bioRxiv - Genetics 2022Quote: ... with the RNase-Free DNase Set (Qiagen, 79254). The quality of the RNA was assessed using the Agilent TapeStation system and quantified by Nanodrop ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was isolated using the miRNeasy Micro Kit from Qiagen and the RNase-free DNase set from Qiagen according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2024Quote: ... and purified using RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... following DNA digestion (RNase-free DNase set, Qiagen) and by using a Qiacube instrument ...
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bioRxiv - Immunology 2023Quote: ... with the RNase-Free DNase Set (Qiagen #79254) to remove any residual DNA ...