Labshake search
Citations for Qiagen :
701 - 750 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: ... after separation of DNA strands and release of samples into the Q24 plate (Qiagen) using PyroMark workstation (Qiagen) ...
-
bioRxiv - Cell Biology 2022Quote: ... single cells were sorted into PCR plates containing 5 µl Buffer RLT Plus (Qiagen) with 1% BME and immediately frozen at -80°C for G&T sequencing.
-
bioRxiv - Cancer Biology 2019Quote: ... immune and non-immune single cells into 96-well plates containing TCL buffer (Qiagen) with 1% beta-mercaptoethanol ...
-
bioRxiv - Genomics 2021Quote: ... Colonies were allowed to grow up overnight on Carbenicillin plates and midiprepped (Qiagen, 12945). We collected approximately 1 million colonies ...
-
bioRxiv - Genetics 2019Quote: HEK293Ts were plated in 6 well plates and transfected using Effectene Transfection Reagent (Qiagen) according the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were sorted into 384 well plates each containing 3 μl Vapor-Lock (Qiagen) in all wells and eight different conditions of 0.3 μl lysis buffer consisting of 0.125μM OligodT30VN (5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’ ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were removed from the plate with 250µL of RNAprotect Cell Reagent (Qiagen 76104) and stored at -20°C for at least 24 hours prior to extraction ...
-
bioRxiv - Cell Biology 2023Quote: ... flow sorted into 96 well plates containing 2.5ul of RLT plus lysis buffer (Qiagen), collecting only viable singlets ...
-
bioRxiv - Genomics 2023Quote: ... Colonies were allowed to grow up overnight on Carbenicillin plates and midiprepped (Qiagen, 12945). For HepG2 and K562 pilot libraries ...
-
bioRxiv - Bioengineering 2023Quote: ... hMSCs were seeded in 12-well plates and transfected with MALAT1 antisense probe (Qiagen) at a concentration of 50 nM (final concentration ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10 µM A740003 or both BzATP and A740003 together (A740003 was pre incubated for 1 h before adding BzATP) 24 hours later RNA was extracted using the miRNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: Genomic DNA was extracted from 1 B6 and two Card19lxcn BMDMs derived from three independent mice using a DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: CIRCLE-seq libraries were prepared for each gRNA (IDT) using genomic DNA extracted from NHGRI-1 (DNA Blood & Tissue kit, Qiagen) following the described protocol [59] ...
-
bioRxiv - Microbiology 2020Quote: ... We sampled 1 ml of the clear phase for living planktonic cells and extracted DNA using DNeasy Blood & Tissue Kit (Qiagen). The cultures were also plated on SHIEH-agar plates with dilutions for cfu/ml -estimation and colony-PCR.
-
bioRxiv - Genomics 2020Quote: PCR amplicons verified by 1% agarose gel electrophoresis were purified from gel using Gel extraction kit (Qiagen GmbH, Hilden, Germany) and ligated into a pGEM-T easy cloning vector (Promega ...
-
bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from cavin1a/1b DKO and WT zebrafish embryos (> 100 embryos randomly selected from 1 clutch) using the RNeasy Mini Kit (QIAGEN) and cDNA synthesis was performed using SuperscriptIII reverse transcriptase (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... total RNA was isolated from 1×106 BMMCs that were untreated or treated with IgE receptor crosslinking using the RNeasy mini kit (Qiagen). RNA was treated with DNase I according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... plasmids containing qPCR amplicon sequences were linearized by digestion with SmaI for 1 h and purified using the QIAquick PCR purification kit (Qiagen). Linearized plasmids were quantified using spectrophotometry ...
-
bioRxiv - Molecular Biology 2021Quote: The ER-I-PpoI cells treated with 4-OHT (2.5 μM) for 1 hour were collected for extraction of the total nucleic acids using DNeasy Blood & Tissue Kits (Qiagen) or PureLink(tm ...
-
bioRxiv - Genomics 2019Quote: Total RNAs from the knockdown (shCTCF#1 and shCTCF#2) and control (shLuc) cells were extracted using miRNeasy Micro Kit (QIAGEN). cDNA was synthesized by using oligo (dT)20 and SuperScript III Reverse Transcriptase (Invitrogen ...
-
bioRxiv - Genetics 2019Quote: ... The resulting products were then either treated with DpnI or purified from a 1% agarose gel with a QIAquick Gel Extraction Kit (Qiagen) and transformed into E.coli DH5α competent cells (Invitrogen ...
-
bioRxiv - Microbiology 2019Quote: ... viral DNA was either extracted using modified phenol/chloroform extraction routes (Figure 1, Route A-D) or QIAamp Viral RNA Mini Kit [22] (Qiagen) according manufacturer’s instructions (Figure 1 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Pellet was treated with 20 μg lysostaphin (1 mg/ml) and RNA isolation was performed using the RNeasy Plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: Around 250 mg of each produce sample was cut into 1-3 mm pieces using a scalpel and then processed with the DNeasy Powersoil Pro kit (Qiagen) to isolate 50μL of eluted DNA ...
-
bioRxiv - Microbiology 2019Quote: ... total RNA was extracted from 1∼5 million PBMCs into 30ml of water with RNeasy Mini Kits (Qiagen, Valencia, CA). For unbiased repertoire analysis ...
-
bioRxiv - Immunology 2021Quote: ... The PCR products were subjected to 1 % agarose gel electrophoresis for analysis and purified with QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2021Quote: ... a volume equivalent to 5×108 cells were spun down (assuming 1 OD600=8×108 cells) and Protect Bacteria RNA Mini Kit (Qiagen) was used to extract total RNA as described in (Bhattacharyya et al. ...
-
bioRxiv - Cell Biology 2021Quote: RNA samples of MS-5 cells cultured alone and in co-culture with SKM-1 cells extracted using Trizol were purified using the Rneasy Plus Micro kit (Qiagen) according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... Endogenous Mecp2 cDNA was amplified for analysis using a forward primer in the 5’ untranslated region and a reverse primer located in the 3’ untranslated region PCR products were fractionated on a 1% agarose gel and purified using the QIAquick gel extraction kit (Qiagen) before being submitted for Sanger sequence analysis ...
-
bioRxiv - Plant Biology 2021Quote: RNA was extracted by two different approaches: 1) for RNASeq: RNA was extracted using a column based kit from QIAGEN GmbH (Plant RNeasy ...
-
bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
-
bioRxiv - Systems Biology 2021Quote: ... 0.5-1 μg of total RNA was reverse transcribed into first-strand cDNA using an RT2 First Strand Kit (QIAGEN). The resultant cDNA was subjected to qPCR using human cytokine-specific primer (Realtimeprimers.com ...
-
bioRxiv - Plant Biology 2021Quote: RNA was extracted from root tips (1-2 mm) of 7-d-old seedlings using the RNEasy plant mini kit (QIAGEN), according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2020Quote: ... Cells were seeded in 6-well plates at a density of 1×106 cells/mL and transfected with 200 ng of plasmid using the Effectene transfection kit (Qiagen). Cells were harvested following 3 days of incubation.
-
bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 1 mL digestate slurry or 0.25 g soil using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) following a modified kit protocol where bead beating for 30s at 4.5 ms−1 in a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...
-
bioRxiv - Molecular Biology 2020Quote: RNA was extracted from 14-day-old wild-type and acinus-2 pinin-1 seedlings using RNeasy mini kit (Qiagen) and treated with TURBO DNA-free Kit (Ambion ...
-
bioRxiv - Genomics 2019Quote: ... AML cell lines (ML2 and Monomac-1) and AML patient (AML1) was isolated by using the column-based Qiagen DNeasy Blood & Tissue DNA extraction kit (Qiagen) following the manufacturer’s instructions and DNA content was measured with a Qubit 2.0 Fluorometer (Thermo Fisher).
-
bioRxiv - Cancer Biology 2022Quote: RNA was purified from Myc-CaPWT or Myc-CaPΔRb cells treated with vehicle or 1μM JQ-1 for 24 hours using the RNEasy kit (Qiagen, Hilden, Germany). For NanoString analysis ...
-
bioRxiv - Genomics 2022Quote: ... Formalin-Fixed Paraffin-Embedded (FFPE) tissue (3 samples) and fresh frozen tissue (1 sample, QiaAmp Blood mini kit, QIAcube, QIAgen). Two samples are reference samples (NA24385 and NA12892 ...
-
bioRxiv - Microbiology 2022Quote: ... were inoculated in 20 mL tryptone soy broth and incubated at 37°C for 24 h before 1 mL was pelleted and DNA extracted using the DNeasy Blood & Tissue kit (Qiagen). Libraries for genome sequencing were prepared using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs ...
-
bioRxiv - Cancer Biology 2022Quote: Spheroid and monolayer samples (technical replicates n=3) of MUC-1 and NCI-H295R cells were processed for RNA extraction using the RNeasy Mini kit (Qiagen), followed by DNA removal (TURBO DNA-free™ Kit ...
-
bioRxiv - Genomics 2022Quote: PCR amplicons verified by 1% agarose gel electrophoresis were purified from gel using Gel extraction kit (Qiagen GmbH, Hilden, Germany) and ligated into a pGEM-T easy cloning vector (Promega ...
-
bioRxiv - Neuroscience 2021Quote: ... cDNA was generated from CSF isolated RNA and a control RNA sample spiked with miR-39 (1 × 108 copies/ul) using the miScript HiSpec kit (Qiagen); dilutions were created from the control cDNA sample spiked with miR-39 (1 ×106-1×103 copies ...
-
bioRxiv - Microbiology 2021Quote: ... (1 μl) for 18 h and the RNA was extracted by using the mirCury cell and plant isolation kit (QIAGEN). A final number of 108 treated cells were lysed using 500 μL of glass beads and the 600 μL of the Lysis buffer provided with the kit ...
-
bioRxiv - Microbiology 2021Quote: ... PCR amplifications were carried out in 50-μL total volumes containing 1 μL of template using a DNA HotStarTaq Master Mix kit (Qiagen). The cycling protocol was as follows ...
-
bioRxiv - Plant Biology 2021Quote: ... and sesame (cv. Kanto-1) using an RNeasy Plant Mini Kit™ according to the manufacturer’s instructions (Qiagen, https://www.qiagen.com). Reverse transcription of RNAs were carried out using PrimeScript II 1st strand cDNA Kit™ (Takara ...
-
bioRxiv - Cell Biology 2021Quote: ... The extracellular RNAs from 1 mL CPB patient plasma samples were isolated using the exoRNeasy Midi kit (Qiagen, Cat# 77044).
-
bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from either DF-1 cultures infected with the WT virus or DF-1P cells using the Qiamp viral RNA mini kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.2 μM gene specific primers pairs (hVDAC1, hVDAC2, hVDAC3, β-actin (Table 1) and 12.5 μl of master mix (QuantiFast SYBR Green PCR kit, Qiagen). Three independent experiments of quantitative real-time were performed in triplicate for each sample ...
-
bioRxiv - Microbiology 2021Quote: ... The digested DNA was separated on a 1% agarose gel and the ∼5 Kb genomic DNA band was harvested with a QIAquick Gel Extraction Kit (Qiagen). The DNA library was prepared according to the manual of the Ligation Sequencing Kit (Nanopore) ...