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Citations for Qiagen :
451 - 500 of 10000+ citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... RNA was extracted from more than 1 × 107 cells using RNeasy Plus Mini Kit (Qiagen, Valencia, CA). Three biological replicates were prepared for each condition ...
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bioRxiv - Neuroscience 2020Quote: ... DNA was isolated from 1-2 mm tail tissue using the DNeasy Blood and Tissue Kit (Qiagen) following the protocol for isolation of DNA from animal tissues and was eluted in 100 µl H2O ...
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bioRxiv - Immunology 2020Quote: ... Isolation of RNA from THP-1 cells was carried out with a RNeasy Plus Mini kit (Qiagen). The High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... In brief we perform bisulfite conversion on 1 µg of genomic DNA using EpiTech kit from Qiagen according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5ug of the plasmid of choice was transfected into HEK293T cells together with 1 ug pVSV-G and 1.5 ug pCMVΔR8.2 using an Effectene transfection kit (Qiagen). Twenty-four hours after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting products were run on 1 % agarose gel then extracted using QIAquick Gel Extraction Kit (Qiagen). Restriction enzymes and Phusion DNA polymerase were purchased from New England BioLabs Inc and used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... cDNA was prepared from 0.5-1 μg total RNA with the QuantiTect reverse transcription kit (205311; Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: The small RNAs from the yeast cells (1 x 107) were isolated with the miRNeasy kit (Qiagen) with modifications ...
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bioRxiv - Immunology 2021Quote: ... Cells were sorted in RTL Lysis Buffer plus 1% β-mercaptoethanol (74134, RNeasy Plus Mini Kit; QIAGEN), or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA from 10 Ag(PMB)1 individuals was extracted using the Blood and Tissue Kit (Qiagen). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... RNA extraction was performed from 1 mL of the bacterial solution using an RNeasy Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of gDNA was extracted from WILD-seq transduced cells (Blood&Cell Culture DNA Kit, Qiagen) and the barcode amplified by PCR using primers containing Illumina-compatible adapters (gDNAWhitelist_Fwd/Rev) ...
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bioRxiv - Cell Biology 2022Quote: ... First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen). RT-qPCR was performed in a LightCycler480 (Roche ...
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bioRxiv - Genomics 2022Quote: ... DNA from I-73-1 and D308 was extracted using a ‘Genomic-tip 100/G’ kit (Qiagen) and long-reads sequenced with PacBio RS II at 100x coverage ...
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bioRxiv - Microbiology 2022Quote: ... Ligation of 1 µl 5′RACE PCR products were performed with the Qiagen PCR cloning kit (Qiagen) according to the manufacters protocol ...
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bioRxiv - Immunology 2022Quote: cDNA was generated from 1 µg RNA with the Omniscript® Reverse Transcription Kit (Qiagen, Hilde, Germany) using oligoDT12-18 primer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2024Quote: ... total DNA from 1×106 cells was extracted using the Blood & Cell Culture DNA Mini Kit (Qiagen). Extracted DNA was diluted to 10 ng/μl and subjected to qPCR analysis ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was extracted from 1-5E5 sorted cells using the DNeasy blood and tissue kit (Qiagen, 69504) and used for barcode amplification and NGS analysis.
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of RNA was used per sample using the QuantiTect Reverse Transcription Kit (Cat# 205311, Qiagen). The quantitative RT-qPCR reactions were carried out using the PowerUp™ SYBR™ Green Master Mix (Cat# A25742 ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 min 30 sec at 30 Hz before RNA extraction using the RNeasy micro kit (Qiagen). RNA was sequenced using the DNBseq platform at the Beijing Genomics Institute (BGI Group ...
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bioRxiv - Immunology 2023Quote: ... 1×105 cells were removed four days after electroporation and DNA isolated using the DNeasy kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription was performed from 1 µg RNA using the Quantitect Reverse Transcription Kit (QIAGEN, Manchester, UK) and cDNA was diluted to 10 ng/µl equivalent before use in qPCR ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from cell pellets (1 million cells) using Allprep DNA/RNA mini kit (Qiagen). Whole genome libraries were sequenced 150x2 bp on a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was carried out with 1 µg of RNA using the QuantiTect Reverse Transcription Kit (Qiagen) and subsequently diluted 1:25 in dH2O ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA (gDNA) was extracted from 1×106 PBMCs using the QIAamp DNA Blood Mini Kit (Qiagen) per manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA using a QuantiTect™ Reverse Transcription kit (Qiagen) from random hexamer primers ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of 1 μg of RNA were reverse transcribed using a QuantiTect Reverse Transcription kit (Qiagen, 205314), and qPCR was performed with iTaq SYBR Green (Life Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were confirmed with a 1% agarose gel and PCR purified (QIAquick PCR Purification kit, QIAGEN). The dsRNA was synthesized using a T7 reverse transcription reaction kit (MEGAscript ...
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bioRxiv - Genetics 2024Quote: DNA was isolated using 1 ml from whole blood using the QiAamp DNA blood Midi kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The entire HTT exon 1 coding sequence was amplified using UltraRun® LongRange PCR Kit (QIAGEN, 206442) with 10% DMSO using the following PCR conditions ...
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bioRxiv - Microbiology 2019Quote: ... and the 6xHis-SUMO tagged proteins were purified from the soluble protein fraction after centrifugation using an Ni2+-NTA Superflow column (Qiagen, Venlo, Netherlands). Next ...
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bioRxiv - Cancer Biology 2021Quote: The recombinant hexahistidine-tagged TNC-C and EDB WT and mutant proteins (at 60 μg of protein / 40 μl beads in PBS) were immobilized to Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at RT for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... His-tagged proteins were purified on NiNTA beads (Qiagen). Purified proteins were eluted with 500 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were purified with Ni-NTA affinity resin (Qiagen). The aminoacylation assay protocol from Jiongming Lu was then followed (Lu et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Protein were purified using Ni-NTA agrose (Qiagen, 30210) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2021Quote: ... Soluble protein was mixed with Ni-NTA resin (Qiagen) for 1h at 4 degrees on a nutator ...
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bioRxiv - Biochemistry 2020Quote: ... Fusion proteins were purified through consecutive Ni-NTA (Qiagen), amylose (NEB) ...
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bioRxiv - Biophysics 2021Quote: ... purified N protein was incubated with RNAse A (Qiagen) with 1:15 (RNAse A ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were either purified using NI-NTA agarose (Qiagen) or anti C-tag beads ...
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bioRxiv - Plant Biology 2021Quote: ... labeled proteins were incubated with Ni-NTA agarose (Qiagen) on a rotary shaker for 1 h at RT ...
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bioRxiv - Plant Biology 2021Quote: ... recombinant protein and purified using Ni-NTA resin (Qiagen). Polyclonal antibodies were raised in mice as described in 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant protein was purified using Ni-NTA agarose (Qiagen). Rabbit polyclonal antibodies were generated by Covance ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified by Ni-NTA agarose (Qiagen) and eluted with lysis buffer containing 300 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: Protein was purified using Ni-NTA agarose column (Qiagen). A detailed protocol is described in supplementary materials and methods.
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bioRxiv - Neuroscience 2020Quote: ... GAPDH protein was purified by Ni-NTA chromatography (Qiagen) under native conditions ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were purified using Ni-NTA resin (Qiagen, Germany) on a gravity flow column followed by size-exclusion chromatography on a Superdex® 200 Increase 10/300 GL column (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... Protein purification was performed using Ni-NTA resin (Qiagen) equilibrated with the lysis buffer containing 20 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were purified by Nibaffinity (Ni-NTA agarose, Qiagen) then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Genomics 2019Quote: ... We added 200 µl of Protein Precipitation Solution (Qiagen) and centrifuged at 15,000 rpm for 3 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Genes for protein purification were cloned into pQE30 (QIAGEN). Raw 264.7 and U937 cells were cultured in RPIM1640 medium in the presence of 10% FBS ...