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Citations for TianGen Biotech :
1 - 50 of 75 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... 10* PCR reaction buffer 5µl (Tiangen Biotech, Beijing), dTNPs 4µl ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed with a reaction volume of 25 µl containing 2.5 µl buffer 109 (Tiangen, Beijing, China), 200 µM each dNTP ...
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bioRxiv - Microbiology 2019Quote: ... PCR screening employed the PCR master mix (Tiangen) with the following thermocycling conditions ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... The PCR was performed in triplicate using PCR Mix (TianGen, Beijing) with Fluc primers at 94 °C for 5 min ...
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bioRxiv - Genetics 2022Quote: ... PCR reactions were performed by using 2×Taq PCR Master Mix (TIANGEN, China). KASP marker analysis was performed on the StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... A 412 bp TYLCV fragment was PCR amplified using Taq PCR MasterMix (Tiangen, KT201) and primers V61 and C473 as previously described (Atzmon et al. ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA was synthesized for PCR reaction using a PCR master mix (Tiangen, Beijing, China). The specific amplicons were cloned into pMD18T vectors (TaKaRa ...
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bioRxiv - Microbiology 2019Quote: ... PCR mixture contained 12 μL of 2× Taq PCR Mix (TIANGEN Co. Ltd., Beijing, PR China), 1 μL DNA template ...
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bioRxiv - Evolutionary Biology 2023Quote: ... directly pipetted into a 25-μl PCR reaction containing a 2x concentration of PCR MasterMix (Tiangen) and 10 pmol of each PCR primer ...
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bioRxiv - Microbiology 2024Quote: ... The real-time quantitative PCR (q-PCR) (ABI7500, USA) using SYBR 2×RealUniversal PreMix (Tiangen Biotech). qPCR was performed to assess the abundance of the macrolide ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in a 20-μL reaction containing SYBR Green (Tiangen, China) on an MXP3000 cycler (Stratagene ...
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bioRxiv - Molecular Biology 2020Quote: ... quantitative real-time PCR (qRT-PCR) was performed with SYBR green as fluorescence dye (Cat# FP205-02, Tiangen Biotech, China) in a total reaction volume of 20 μL according to the standard manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 ul 2×Taq PCR mix (TIANGEN, China), 0.5 ul each primer (trnL and trnF (Taberlet et al. ...
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bioRxiv - Microbiology 2021Quote: ... 2×Taq PCR Mix was purchased from TIANGEN Biotech (Beijing ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 RBD-specific quantitative reverse transcription-PCR (qRT-PCR) assays were performed by using a FastKing One Step Probe RT-qPCR kit (Tiangen Biotech, China) on a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 RBD-specific quantitative reverse transcription-PCR (qRT-PCR) assays were performed by using a FastKing One Step Probe RT-qPCR kit (Tiangen Biotech, China) on a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... and the Q-PCR kit was purchased from Tiangen Biotech Co. ...
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bioRxiv - Genetics 2019Quote: ... the PCR amplicons were purified using Gel Extraction kit (TIANGEN). A total of 200 ng of the purified PCR amplicon was denatured and slowly reannealed to facilitate heteroduplex formation ...
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bioRxiv - Genomics 2019Quote: ... The PCR products were cloned into pLB vectors (TianGen, China) and sequenced with an ABI 3730XL 96-capillary DNA analyzer (Lifetech ...
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bioRxiv - Genetics 2022Quote: Cells were directly lysed into RLT plus buffer (TIANGEN, DP430) for total RNA extraction according to the manufacturer’s instructions provided in the RNeasy Plus Mini Kit ...
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... DNA was purified using a PCR Purification Kit (Tiangen,Beijing,China) and subsequently analyzed by qPCR using primers located on the Acox1 and Cpt1a promoter.
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bioRxiv - Genetics 2020Quote: ... and 10 μL real-time PCR SYBR Green (TIANGEN, Beijing, China). Amplification conditions were ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR was performed using SYBR Green Pre-Mix (TIANGEN, FP205) on the Roche LightCycler480 instrument ...
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bioRxiv - Immunology 2022Quote: ... Catch buffer: to 1 ml of RNAase-free water (Tiangen Biotech), add 40 µl Rnasin (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... Both digested products were then cleaned with a PCR purification column (TIANGEN), and ligation was then performed using the T4 DNA ligase (NEB) ...
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bioRxiv - Genomics 2019Quote: ... Erythrocytes were cleared by resuspending the cells in ACK lysis buffer (Tiangen) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR was performed in triplicate with FastFire qPCR PreMix (SYBR Green) (Tiangen) on ViPlex Fluor Real-time PCR system (Vivantis ...
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bioRxiv - Immunology 2020Quote: ... PCR products underwent agarose electrophoresis and gel-purification (Universal DNA purification kit, Tiangen). The amount of PCR products was determined by OD 260 absorption value ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative RT-PCR was performed using the SuperReal PreMix Color (Tiangen, Beijing, China) in the CFX96 real-time system (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were merged and purified by using TIANquick Midi Purification Kit (TIANGEN). The NGS libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative RT-PCR was performed using SYBR Green Master Mix (Tiangen, Beijing, China). The RNA expression level of target genes was evaluated by 2−ΔΔCt.
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bioRxiv - Microbiology 2020Quote: ... were detected by qRT-PCR using SuperReal PreMix Plus (SYBR Green) (Tiangen Biotech, China). As an endogenous control ...
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bioRxiv - Plant Biology 2021Quote: ... The qRT-PCR was completed with the FastKing RT Kit (with gDNase) (TIANGEN, Beijing) and the QuantStudio™ 7 Flex Real-Time PCR System (Life Technologies Corporation ...
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bioRxiv - Genomics 2019Quote: ... the total RNAs were reverse-transcribed into cDNA via RT-PCR (TIANGEN, KR103-04), and the targeted locus was PCR amplified with the corresponding primers (listed in the Supplementary Table 4) ...
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bioRxiv - Genomics 2019Quote: ... the total RNAs were reverse-transcribed into cDNA via RT-PCR (TIANGEN, KR103-04), and the expression of IFN-β and IL-6 were measured by quantitative PCR (TAKARA ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR was completed with the FastKing RT Kit (with gDNase) (TIANGEN, Beijing) and the QuantStudio™ 7 Flex Real-Time PCR System (Life Technologies Corporation ...
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bioRxiv - Molecular Biology 2021Quote: ... The quantitative PCR was performed using Quantagene q225 (KUBOTECHNOLOGY) with SuperReal PreMix Plus (TIANGEN) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were extracted by a gel extraction kit (TIANGEN, Catalog # DP209-03). Data analysis was performed using MAGeCK.
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bioRxiv - Cell Biology 2019Quote: ... Red blood cells were lysed using RBC lysis buffer (catalogue NO. RT122-02, TIANGEN). After centrifugation (3,000 rpm ...
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bioRxiv - Genomics 2019Quote: ... The PCR products were purified and cloned into the pGM-T vector (TIANGEN, Beijing, China); at least 10 positive plasmid clones were sequenced and analyzed using NCBI BLAST.
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bioRxiv - Bioengineering 2021Quote: ... The quantitative PCR was conducted using the SuperReal PreMix SYBR Green kit (Tiangen Biotech, China) and the CFX96 Real-Time system (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Then the PCR products were purified using the Agarose Gel Extraction kit (TIANGEN, Beijing, China), and transformed into Escherichia coli DH5α ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified and directly recombined by the EasyGeno recombinant clone kits (Cat:VI201, Tiangen). After conventional transformation and monoclonal screening of positive bacteria ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products underwent agarose gel electrophoresis and gel purification (Universal DNA purification kit, Tiangen, Beijing, China). For animal experiments ...
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bioRxiv - Bioengineering 2023Quote: ... the PCR products were purified after agarose electrophoresis (using Universal DNA purification kit, TIANGEN, Beijing, China), and their concentrations were determined by the OD 260 value by NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The sgRNA-targeted loci of Lmna gene were amplified using Taq PCR MasterMix (KT211, Tiangen, China) and purified by TIANgel Purification Kit (DP219 ...
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bioRxiv - Molecular Biology 2023Quote: ... The sgRNA-targeted loci of Palmd gene were amplified using Taq PCR MasterMix (KT211, Tiangen, China) and purified by TIANgel Purification Kit (DP219 ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative RT-PCR was conducted in a total 20 μl reaction volume with 10μl SYBR premix (TIANGEN). The relative RNA amounts were calculated with the comparative CT (2-DDCT ...
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bioRxiv - Microbiology 2020Quote: ... One step Qrt-PCR reaction was performed using the Tiangen One-step SYBR Green kit (Tiangen, China) in the Applied Biosystems QuantStudio 6 Flex RT-PCR System.
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...