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Citations for TianGen Biotech :
1 - 50 of 561 citations for Mouse Direct PCR Kit For Genotyping since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... and the Q-PCR kit was purchased from Tiangen Biotech Co. ...
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bioRxiv - Genetics 2019Quote: ... the PCR amplicons were purified using Gel Extraction kit (TIANGEN). A total of 200 ng of the purified PCR amplicon was denatured and slowly reannealed to facilitate heteroduplex formation ...
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... DNA was purified using a PCR Purification Kit (Tiangen,Beijing,China) and subsequently analyzed by qPCR using primers located on the Acox1 and Cpt1a promoter.
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bioRxiv - Immunology 2020Quote: ... PCR products underwent agarose electrophoresis and gel-purification (Universal DNA purification kit, Tiangen). The amount of PCR products was determined by OD 260 absorption value ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were merged and purified by using TIANquick Midi Purification Kit (TIANGEN). The NGS libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 RBD-specific quantitative reverse transcription-PCR (qRT-PCR) assays were performed by using a FastKing One Step Probe RT-qPCR kit (Tiangen Biotech, China) on a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 RBD-specific quantitative reverse transcription-PCR (qRT-PCR) assays were performed by using a FastKing One Step Probe RT-qPCR kit (Tiangen Biotech, China) on a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from mouse brain lysates (mixture of 3∼4 mouse brains for each sample) using RNAprep pure Tissue Kit (Tiangen, DP431). cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (Vazyme ...
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bioRxiv - Molecular Biology 2019Quote: ... we used a miRcute miRNA first-strand cDNA synthesis kit and a miRcute miRNA qRT-PCR detection kit (SYBR Green) (TIANGEN). The miRNA-specific forward primers used for qRT-PCR are also shown in Table S2.
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bioRxiv - Plant Biology 2021Quote: ... The qRT-PCR was completed with the FastKing RT Kit (with gDNase) (TIANGEN, Beijing) and the QuantStudio™ 7 Flex Real-Time PCR System (Life Technologies Corporation ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR was completed with the FastKing RT Kit (with gDNase) (TIANGEN, Beijing) and the QuantStudio™ 7 Flex Real-Time PCR System (Life Technologies Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were extracted by a gel extraction kit (TIANGEN, Catalog # DP209-03). Data analysis was performed using MAGeCK.
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bioRxiv - Bioengineering 2021Quote: ... The quantitative PCR was conducted using the SuperReal PreMix SYBR Green kit (Tiangen Biotech, China) and the CFX96 Real-Time system (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Then the PCR products were purified using the Agarose Gel Extraction kit (TIANGEN, Beijing, China), and transformed into Escherichia coli DH5α ...
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bioRxiv - Neuroscience 2022Quote: Genomic DNA was extracted from mouse brain using TIANamp Genomic DNA Kit (TIANGEN, DP304) following the recommended protocol ...
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bioRxiv - Microbiology 2019Quote: ... PCR screening employed the PCR master mix (Tiangen) with the following thermocycling conditions ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified and directly recombined by the EasyGeno recombinant clone kits (Cat:VI201, Tiangen). After conventional transformation and monoclonal screening of positive bacteria ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products underwent agarose gel electrophoresis and gel purification (Universal DNA purification kit, Tiangen, Beijing, China). For animal experiments ...
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bioRxiv - Bioengineering 2023Quote: ... the PCR products were purified after agarose electrophoresis (using Universal DNA purification kit, TIANGEN, Beijing, China), and their concentrations were determined by the OD 260 value by NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Mouse DNA was extracted from the liver using an extraction kit (DP341-01, Tiangen, China) as per the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... One step Qrt-PCR reaction was performed using the Tiangen One-step SYBR Green kit (Tiangen, China) in the Applied Biosystems QuantStudio 6 Flex RT-PCR System.
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bioRxiv - Immunology 2022Quote: ... cDNA synthesis was executed with 1 µg of total RNA with BcaBEST RNA PCR Kit (TIANGEN, China) as the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Follow the reverse transcription box instructions for reverse transcription (FastKing One Step RT-PCR Kit, KR123, Tiangen). Real-time PCR (Talent qPCR PreMix (SYBR Green) ...
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bioRxiv - Immunology 2019Quote: ... the products of first round extension PCR were purified by gel extraction kit (TIANGEN Biotech, Beijing, China), of which 5 μL of each sample was used as the template in the second extension PCR ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were gel purified and cloned with the TIAN Quick Midi Purification Kit (Tiangen, Beijing, China) following the manufacturer’s recommendations ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed SuperReal PreMix Plus kit (Tiangen Biotech, China) by ABI 7500 Q-PCR system (Applied Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... The genome extraction from mouse embryos or pups were using the TIANamp Genomic DNA Kit (TIANGEN), which used for genotyping or bisulphite sequencing.
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bioRxiv - Cancer Biology 2023Quote: ... and the total RNA of mouse thyroid tissues was isolated with the Tiangen Kit (Tiangen, DP419), then reverse-transcribed to cDNA using the One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified from agarose gels using a TIANgel Purification Kit (TianGen BioTech Co. Ltd, China), followed by nucleotide sequencing performed by Sangon Biotech (Shanghai ...
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bioRxiv - Genetics 2021Quote: ... PCR products from each sample were separately pooled and purified with a Universal DNA Purification Kit (Tiangen, China). In the second-round PCR ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... The PCR was performed in triplicate using PCR Mix (TianGen, Beijing) with Fluc primers at 94 °C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and dH2O in a 25 μl volume using a QuantiTect SYBR Green PCR kit(TIANGEN BioTech Co.LTD, Beijing, China).Transcripts were quantified with β-actin as an internal standard ...
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bioRxiv - Developmental Biology 2019Quote: ... Real-time PCR reactions were carried out with a Real Master Mix SYBR Green Kit (Tiangen, Corp, Beijing, China) using a Stratagene Mx300p (Agilent Technologies Inc ...
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bioRxiv - Genetics 2019Quote: The single-stranded viral DNA was isolated from M13mp18 and its 3kb linear dsDNA was amplified by PCR and purified by DNA purification kit (Tiangen). A 60-nt oligomer from M13 genome ...
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bioRxiv - Genetics 2022Quote: ... PCR reactions were performed by using 2×Taq PCR Master Mix (TIANGEN, China). KASP marker analysis was performed on the StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from mouse testes using an RNA Easy Fast Tissue/Cell Kit (Tiangen Biotech, China, 4992732). Approximately 0.3 mg of total RNA was converted into cDNA with the PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Microbiology 2021Quote: ... A 412 bp TYLCV fragment was PCR amplified using Taq PCR MasterMix (Tiangen, KT201) and primers V61 and C473 as previously described (Atzmon et al. ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription and quantitative real-time PCR assay were performed in a Bio-Rad iQ5 real-time system using a Quant One Step qRT-PCR (SYBR Green I) Kit (Tiangen Biotech). Transcript expression levels of FmASP were normalized to that of the reference gene ACTIN and calculated using the 2-ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA was synthesized for PCR reaction using a PCR master mix (Tiangen, Beijing, China). The specific amplicons were cloned into pMD18T vectors (TaKaRa ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products underwent agarose electrophoresis and gel-purification to remove plasmid template and free dNTPs (Universal DNA purification kit, Tiangen, Beijing, China). The amount of PCR products was determined by the OD 260 absorption value ...
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bioRxiv - Microbiology 2019Quote: ... PCR mixture contained 12 μL of 2× Taq PCR Mix (TIANGEN Co. Ltd., Beijing, PR China), 1 μL DNA template ...
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bioRxiv - Evolutionary Biology 2023Quote: ... directly pipetted into a 25-μl PCR reaction containing a 2x concentration of PCR MasterMix (Tiangen) and 10 pmol of each PCR primer ...
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bioRxiv - Microbiology 2024Quote: ... The real-time quantitative PCR (q-PCR) (ABI7500, USA) using SYBR 2×RealUniversal PreMix (Tiangen Biotech). qPCR was performed to assess the abundance of the macrolide ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The PCR products were purified by excision of the band from agarose gel using the Universal DNA Purification Kit (TIANGEN Bio, Beijing, China), and ligated into a pGEM®-T vector (Promega Co. ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in a 20-μL reaction containing SYBR Green (Tiangen, China) on an MXP3000 cycler (Stratagene ...
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bioRxiv - Molecular Biology 2020Quote: ... quantitative real-time PCR (qRT-PCR) was performed with SYBR green as fluorescence dye (Cat# FP205-02, Tiangen Biotech, China) in a total reaction volume of 20 μL according to the standard manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 ul 2×Taq PCR mix (TIANGEN, China), 0.5 ul each primer (trnL and trnF (Taberlet et al. ...
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bioRxiv - Bioengineering 2020Quote: ... 10* PCR reaction buffer 5µl (Tiangen Biotech, Beijing), dTNPs 4µl ...
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bioRxiv - Microbiology 2021Quote: ... 2×Taq PCR Mix was purchased from TIANGEN Biotech (Beijing ...
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bioRxiv - Genomics 2019Quote: ... The PCR products were cloned into pLB vectors (TianGen, China) and sequenced with an ABI 3730XL 96-capillary DNA analyzer (Lifetech ...