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Citations for TianGen Biotech :
551 - 600 of 605 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: Total RNA was isolated from cultured cells according to the protocols of manufacturers (Tiangen, Beijing, China). cDNA was subsequently synthesized by using a cDNA reverse transcription kit (Toyobo ...
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bioRxiv - Plant Biology 2020Quote: Total RNA samples extracted from M82 and yft1 pericarp (47 dpa, BR stage) using the RNAprep pure Plant Kit (Tiangen, Beijing, China) were used as a templates in rapid amplification of cDNA ends (RACE ...
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bioRxiv - Plant Biology 2020Quote: ... AAA-CAA) within the PGEX-6P-1 plasmid was performed using the fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Microbiology 2021Quote: ... of strain TC967 was extracted from 4-day-old mycelia following the protocol described in the handbook of the Plant Genomic DNA Kit (Tiangen, Beijing, China). The gDNA was sequenced by Illumina HisSeq 2500 sequencing (Illumina ...
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bioRxiv - Genetics 2022Quote: ... First-strand cDNA synthesis from the total RNA was performed by using the FastKing RT kit (with gDNase) (TianGen Biotech Co, Beijing). The expression analyses were performed using the primer set (F-GGACTTCCCTTGGATCCTGC and R-ACCGACAATCATGTCCGCAT) ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from single third-instar larva of three strains (total 30 larvae of each strain) using the DNA/RNA/Protein Isolation Kit (TianGen, Beijing, China). The first-strand cDNA was synthesized with 1 μg of total RNA using a PrimeScript™ 1st Strand cDNA Synthesis Kit with gDNA Eraser (TaKaRa Biotechnology ...
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bioRxiv - Genetics 2019Quote: ... and the whole genome DNA of the patients was extracted using DNA extraction kit (product of Beijing Tiangen Biochemical Technology Co., Ltd.) and using BloodGen Midi Kit (CWBIO ...
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bioRxiv - Developmental Biology 2020Quote: ... All RT-qPCR assays were performed in an ABI 7500 System with the SuperReal PreMix Plus kit (SYBR Green) (Tiangen, Beijing, China). All RT-qPCR procedures for each gene were performed in three biological replicates ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Synthetic Biology 2021Quote: ... The incubated solution is then subjected to agarose gel electrophoresis and the correct band is extracted using a TIANgeo Midi Purification Kit (TIANGEN, DP209-02) or AxyPrep DNA gel Extraction Kit (AXYGEN ...
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bioRxiv - Plant Biology 2020Quote: RNA was extracted according to the instructions of the RNA Isolation Kit for polyphenol- and polysaccharide-rich plants (Tiangen Biotech, Beijing, China). The reverse transcription primer was Oligo(dT)18 ...
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bioRxiv - Plant Biology 2020Quote: ... MG), 47 dpa(Breaker stage, BR) and 54 dpa(Red Ripe stage, RR) using the RNAprep pure Plant Kit (Tiangen, Beijing, China) based on the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 µg) was used as a template for cDNA synthesis with a QuantScript RT Kit (KR103; Tiangen Biotech, Beijing, China). To determine mRNA expression levels ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products underwent agarose electrophoresis and gel-purification to remove plasmid template and free dNTPs (Universal DNA purification kit, Tiangen, Beijing, China). The amount of PCR products was determined by the OD 260 absorption value ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was extracted from the 2nd pair or 3rd pair of leaf counted from the top following the protocol of TIANquick Midi Purificatioin kit (TIANGEN Biotech, Beijing). The primers were listed in Table S2.
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA has been extracted from putative transformants one-month-old leaves grown on a kanamycin-containing medium using TianGen kits (TianGen BioTech, China). The quality of the extracted genomic DNA (250–350 ng/μl ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from peripheral blood samples collected from all the participants using the blood DNA extraction kit (TianGen Biotech, China). We performed the whole genome sequencing on Illumina NovaSeq 6000 system (150 bp paired-end reads ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from blood samples collected from the wing vein using a DNA extraction mini kit (Tiangen Biotech, Beijing, China). All sampled hens were killed by cervical dislocation immediately after oviposition and the abdominal cavity was opened ...
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bioRxiv - Plant Biology 2022Quote: ... and total mRNA was extracted using the RNA Prep Pure Plant Plus Kit according to the manufacturer’s instructions (Tiangen Biotech, Beijing, China). A strand-switching method and the cDNA-PCR Sequencing Kit (SQK-PCS109 ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA was isolated from the cecal contents using the magnetic-based soil and stool DNA extraction kit (TIANGEN®, Beijing, China). The concentration of the extracted DNA was determined using the Qubit Fluorometric Quantitation system (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... the DNA fragments were separated by 1.0 % agarose gel electrophoresis (1 × TAE buffer) and purified from the gel with the DNA Gel Extraction Kit (TianGen Biotech., Beijing, China), then they were sub-cloned into pCE2 TA/Blunt-Zero vector (Vazyme ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 RBD-specific quantitative reverse transcription-PCR (qRT-PCR) assays were performed by using a FastKing One Step Probe RT-qPCR kit (Tiangen Biotech, China) on a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 RBD-specific quantitative reverse transcription-PCR (qRT-PCR) assays were performed by using a FastKing One Step Probe RT-qPCR kit (Tiangen Biotech, China) on a CFX96 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: Total RNAs were isolated from all samples with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO. ...
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bioRxiv - Genomics 2024Quote: ... total RNA of EPCs treated with NC or Tfeb-si1 were extracted using the RNAsimple Total RNA Kit (TIANGEN, cat. no. DP419). Library construction was performed using the SHERRY protocol as described previously [86] ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted from ‘Nagafu No.2’ (standard-type) and ‘Yanfu No.6’ (spur-type) leaves using a Super Plant Genomic DNA Kit (TIANGEN, DP360, China). Approximately 300 ng of DNA was treated with bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research ...
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bioRxiv - Zoology 2024Quote: ... The RT-qPCR reactions were carried out on the PikoReal 96 Real-Time PCR System (Thermo) using the SuperReal PreMix Color (SYBR Green) kit (Tiangen, Beijing, China). Three technical replicates were applied for each biological replicate ...
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bioRxiv - Genomics 2019Quote: ... cells were sorted and collected by FACS assay and were then subjected to RNA isolation (TIANGEN, DP420). Then ...
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bioRxiv - Genetics 2022Quote: ... The CDS was cloned into the MCS of the modified pWMB110 vector under the ubiquitin promoter by using the EasyGeno Assembly Cloning kit (TianGen Biotech Co, Beijing). The recombinant plasmid was transformed into Agrobacterium strain C58C1 (Zoman Biotech Co ...
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bioRxiv - Molecular Biology 2019Quote: Total RNAs were isolated from intermuscular fat tissue by RNAprep Pure Tissue Kit following the manufacturer’s instructions (Tiangen Biotech Co., Ltd., Beijing, China). The purity ...
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bioRxiv - Pathology 2021Quote: ... Total RNA was extracted from mycelia of transformants cultured in V8 juice medium by using an RNA-simple Total RNA Kit (Tiangen Biotech, Beijing, China). PsFYVE1 specific primers (S5 Table ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was extracted from the leaves of T3 transgenic plants (Table S8, lines 1954-1 and 1955-3) using a plant genomic DNA Extraction Kit (Tiangen Biotech, Beijing, China). About 20 μg of DNA was successfully digested with 5 U of EcoRV and incubated at 37 °C for 24 h ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from leaves of transgenic lines and wild-type plants treated with pathogens or hormones using the RNAprep pure plant kit (TIANGEN, DP441, Beijing, China). Then ...
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bioRxiv - Microbiology 2021Quote: The genomic DNA of the B3 strain was extracted using a Bacterial Genomic DNA Purification Kit (DP302-2, Tiangen Biotech Co., Beijing, China) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted from mid-exponential cultures of strain HP1 (both the control and experimental groups) using the RNAprep Pure Bacteria Kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The residual plasmid frame was then inserted by the synthesized combined fragment (GL-SV40pA-pTK-RF-bGHpA) using the EasyGeno Single Assembly Cloning kit (Tiangen Biotech, Beijing, China) to construct the dual-luciferase reporter plasmid (pGL-RF) ...
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bioRxiv - Biochemistry 2019Quote: The extraction of colonic tissues total RNA was according to the manufacture instructions by using RNAprep pure Tissue Kit (TIANGEN BIOTECH, BeiJing, China). After that ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The PCR products were purified by excision of the band from agarose gel using the Universal DNA Purification Kit (TIANGEN Bio, Beijing, China), and ligated into a pGEM®-T vector (Promega Co. ...
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bioRxiv - Biochemistry 2021Quote: Tea plant tissues or root materials were ground in liquid nitrogen into fine powders for total RNA extraction with an RNA extraction kit (Tiangen Biotech Co., Ltd.) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... was isolated from fresh spermatozoa from the WT mice (n=3) and the Iqch KO mice (n=3) using the RNAsimple Total RNA Kit (Tiangen Biotech, China, 4992858). A Ribo-Zero™ rRNA Removal Kit (MRZPL1224 ...
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bioRxiv - Immunology 2019Quote: Plasmids of the four constructed expression vectors were extracted and transformed into Rosetta (DE3) competent cell (TIANGEN, # CB108), respectively ...
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bioRxiv - Microbiology 2020Quote: ... Δ N1-SpyCatcher-I53-50A1.1PT1 and I53-50B.4PT1 was transformed into Rosetta™ (DE3) competent cells (TIANGEN). After incubation for overnight at 37 °C on TB-agars culture plate supplemented with 50 mg/mL kanamycin and 33 mg/mL chloramphenicol ...
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bioRxiv - Plant Biology 2020Quote: ... We performed site-directed mutagenesis of K206 (K206R, AAG-AGG; K206Q, AAA-CAA) within the PET28a plasmid using the Fast Site-Directed Mutagenesis Kit (Tiangen, China, Catalog No. KM101). PCR was conducted using site-specific primers (Supplemental Table S17) ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was isolated from positive samples by lysis with GS buffer containing proteinase K as described in the TIANamp Blood DNA Kit (TIANGEN Biotech Co. Ltd, Beijing) and stored at −20°C ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was isolated from positive samples by lysis with GS buffer containing proteinase K as described in the TIANamp Blood DNA Kit (TIANGEN Biotech Co. Ltd, Beijing) and stored at -20°C ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA of strains and related Pf prophage mutation strains were isolated using the TIANamp Bacteria DNA kit (Tiangen Biotech Co. Ltd, Beijing, China) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μg of purified RNA was reverse-transcribed in a 20 µL reaction using a FastQuant RT Super Mix Kit (TIANGEN, catalog no. KR106-02). Real-Time PCR was then performed by using Maxima SYBR Green/ROX kit (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from tissues of tea plant as well as Arabidopsis thaliana using an RNAprep Pure Plant Kit (Tiangen Biotech Co., Ltd, China) according to the manufacturer’s protocols ...
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bioRxiv - Zoology 2023Quote: Genomic DNA of the strain E2 was extracted using a TIANamp Bacterial DNA Kit according to the manufacturer’s protocol (Tiangen Biotech Co., LTD, Beijing, China). The PacBio and Illumina Miseq sequencing of the strain E2 genome and bioinformatic analysis were performed by Guangdong Magigene Biotechnology Co. ...
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... cDNA synthesis was conducted using 500 ng of DNase-treated RNA and the Talent qPCR Premix (SYBR Green) kit (TIANGEN Biotech Co., Ltd., Beijing), in accordance with the manufacturer’s introductions ...