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Citations for System Biosciences :
1 - 19 of 19 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: To silence miR-424 the GFP tagged lentiviral vectors that express antisense hsa-miR-424 (MZIP424-PA-1) and corresponding scramble control lentiviral vectors (MZIP000-PA-1) were purchased from SBI (System Biosciences). Transfection of 293T packaging cell line was performed using calcium-phosphate protocol and infection of target cells was done as previously described (Platzer et al. ...
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bioRxiv - Neuroscience 2021Quote: ... a commercially available specific primer set was used (System Biosciences). For the detection of some other targets ...
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bioRxiv - Molecular Biology 2020Quote: ... miR-Zip control and miR-Zip-200c plasmids were purchased from System Biosciences.
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase reporter assays were performed by co-transfection of miR-423-5p or miR-Ctrl construct (System Biosciences) with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR ...
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bioRxiv - Molecular Biology 2021Quote: ... a lentiviral Lenti/anti-miR-31 (antimiR-31) was created using commercial plasmids pLenti/anti-miR-31 (System Biosciences, Palo Alto, CA). A Lenti/anti-miR-Cont (antimiR-Cont ...
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bioRxiv - Molecular Biology 2023Quote: ... and miRZip423-5p (anti-miR-423-5p) were purchased from System Biosciences Inc (Palo Alto ...
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bioRxiv - Biochemistry 2023Quote: ... was amplified from human cDNA using PCR primers with XbaI and BamHI overhangs and cloned into XbaI/BamHI digested pCDH-EF1-MCS-Blasticidin (System Biosciences). Standard mutagenesis was used to generate the S251A ...
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bioRxiv - Neuroscience 2022Quote: ... HEK 293T cells cultured in 10-cm Petri dishes at a 50-60% confluence were co-transfected with 10 μg lentiviral plasmid expressing miR-124-1 precursor under the CMV promoter and GFP under the EF1 promoter (SBI System Biosciences) or 10 μg lentiviral plasmid expressing GFP under the CMV promoter and the packaging plasmids pVSV-G (3.5 μg) ...
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bioRxiv - Developmental Biology 2023Quote: ... miRZip anti-miR precursor constructs for miRZip-129-5p and pGreenPuro Scramble Hairpin control constructs were purchased from System Biosciences (Palo Alto, CA), and lentiviruses were generated according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... miRZip anti-miR precursor constructs for miR-322 and miR-503 cluster (miRZIP-322 and miRZip-503) and pGreenPuro Scramble Hairpin control construct were purchased from System Biosciences (Palo Alto, CA), and lentiviruses were generated according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... A forward primer of unknown sequence for XMIR-NT was initially purchased from System Bioscience (Palo Alto, CA, USA), then later custom ordered from IDT after plasmid sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... complementary primers were hybridized and cloned into the BamHI and EcoRI sites of the lentiviral pSIH-H1-copGFP-shRNA vector (System Biosciences) according to the manufacturer’s instructions to generate pSIH-T1 and pSIH-T2 plamsids for constitutive knockdown ...
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bioRxiv - Genetics 2019Quote: PCR amplification was performed according to the PrecisionX Multiplex gRNA Cloning Kit (System Bioscience). BbsI-linearized piggyBac-U6 backbone was mixed with purified PCE product in a vector:insert 2:1 ratio and was directly used for transformation of One Shot MAX Efficiency DH5α-T1 Competent Cells (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... the cyclin D1 was generated by PCR insertion into the XPack CMV constructs (System Biosciences). Hsc70D10N was generated by PCR-based site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) ...
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bioRxiv - Immunology 2024Quote: ... The PCR fragments were cloned into a PiggyBac mammalian expression vector (System Biosciences, PB210PA-1), modified to harbor a Secrecon-AA signal peptide111 for secretion at the N-terminus ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting PCR fragment was inserted by means of the cold fusion reaction (System Biosciences; CA, USA) into the EcoRI-XbaI-digested pCaSpeR-mb247-hs-GCamP3 vector (gift from T ...
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bioRxiv - Developmental Biology 2021Quote: ... The sCMV promoter was amplified from pCS2+ 69 by PCR and cloned into the pCDH-EF1α-MCS-(PGK-GFP-T2A-PURO) bidirectional promoter lentiviral vector (System Biosciences), replacing the PGK promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... pT-mAppleT2Apuro was generated by PCR amplifying the T2A peptide and puromycin resistance gene from PB-CMV-MCS-GreenPuro (System Biosciences, Cat# PB513B-1) and cloning into pT-mApple (Vectorbuilder) ...
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bioRxiv - Cell Biology 2020Quote: ... We PCR amplified the EC-BioID sequence and inserted it into the PiggyBac-GFP vector using NheI site (PB533A, System Biosciences, Mountain View, CA). For C-BioID ...